Restriction map for
pFN21AE5258
(Circular) (Six-base) MAPSORT of: pFN21AE5258.seq Check: 7351 from: 1 to: 5473
pFN21AE5258 5473 bp
With 182 enzymes: SgfI *
December 8, 2014 13:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2688 3283 278
Size: 53 83 186 936 1152 595 2468
Fragments arranged by size:
2468 1152 936 595 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2816 1937
Size: 879 4594
Acc65I G'GTAC_C
Cuts at: 2794 2794
Size: 5473
AclI AA'CG_TT
Cuts at: 1563 2772 3600 3973 1563
Size: 1209 828 373 3063
Fragments arranged by size:
3063 1209 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3536 4920 5188 1632
Size: 1904 1384 268 1917
Fragments arranged by size:
1917 1904 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5276
AflIII A'CryG_T
Cuts at: 1203 2390 4372 5349 1203
Size: 1187 1982 977 1327
Fragments arranged by size:
1982 1327 1187 977
AgeI A'CCGG_T
Cuts at: 4304 4304
Size: 5473
AhdI GACnn_n'nnGTC
Cuts at: 2642 4202 2642
Size: 1560 3913
AleI CACnn'nnGTG
Cuts at: 1166 2579 1166
Size: 1413 4060
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5473
AlwNI CAG_nnn'CTG
Cuts at: 3216 4788 3216
Size: 1572 3901
ApaLI G'TGCA_C
Cuts at: 2088 3530 4686 2088
Size: 1442 1156 2875
Fragments arranged by size:
2875 1442 1156
ApoI r'AATT_y
Cuts at: 1493 2782 3008 3160 5269 1493
Size: 1289 226 152 2109 1697
Fragments arranged by size:
2109 1697 1289 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4027 160
Size: 872 2995 1606
Fragments arranged by size:
2995 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5473
AvaI C'yCGr_G
Cuts at: 1870 1943 2274 2404 2798 1870
Size: 73 331 130 394 4545
Fragments arranged by size:
4545 394 331 130 73
BamHI G'GATC_C
Cuts at: 2803 3170 2803
Size: 367 5106
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2562 2794 4249 618
Size: 325 217 627 775 232 1455 1842
Fragments arranged by size:
1842 1455 775 627 325 232 217
BanII G_rGCy'C
Cuts at: 1391 2474 2792 1391
Size: 1083 318 4072
Fragments arranged by size:
4072 1083 318
BbeI G_GCGC'C
Cuts at: 2566 2566
Size: 5473
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4502
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3664 3698 1903
Size: 34 1727 34 3678
Fragments arranged by size:
3678 1727 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3365 4575 3365
Size: 1210 4263
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5473
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4084 136
Size: 107 122 71 631 605 2412 1525
Fragments arranged by size:
2412 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5468 5468
Size: 5473
BlpI GC'TnA_GC
Cuts at: 2890 2890
Size: 5473
Bme1580I G_kGCm'C
Cuts at: 1790 2092 3534 4690 1790
Size: 302 1442 1156 2573
Fragments arranged by size:
2573 1442 1156 302
BmgBI CAC'GTC
Cuts at: 1318 2271 1318
Size: 953 4520
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2179 3204 4162
Size: 416 53 83 399 24 762 1025 958
Cuts at: 4162 442
Size: 1753
Fragments arranged by size:
1753 1025 958 762 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5473
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2644 2644
Size: 5473
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4133 1239
Size: 2894 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3598 4463 4761 3598
Size: 865 298 4310
Fragments arranged by size:
4310 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4136 882
Size: 617 2637 2219
Fragments arranged by size:
2637 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3264 493
Size: 711 2060 2702
Fragments arranged by size:
2702 2060 711
BsaBI GATnn'nnATC
Cuts at: 5467 5467
Size: 5473
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2563 2685 3280 3662
Size: 53 83 186 936 1030 122 595 382
Cuts at: 3662 4297 275
Size: 635 1451
Fragments arranged by size:
1451 1030 936 635 595 382 186 122
83 53
BsaWI w'CCGG_w
Cuts at: 3906 4304 4578 4725 5136 3906
Size: 398 274 147 411 4243
Fragments arranged by size:
4243 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5248
Fragments arranged by size:
5248 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2006 1186
Size: 820 4653
BseYI C'CCAG_C
Cuts at: 2100 2430 2465 4676 5172 2100
Size: 330 35 2211 496 2401
Fragments arranged by size:
2401 2211 496 330 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2607 1163
Size: 573 871 4029
Fragments arranged by size:
4029 871 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2923 3684 4712 1540
Size: 275 186 922 761 1028 2301
Fragments arranged by size:
2301 1028 922 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2092 2229 3534 3619 4690 2092
Size: 137 1305 85 1071 2875
Fragments arranged by size:
2875 1305 1071 137 85
BsmI GAATG_Cn'
Cuts at: 2984 3077 2984
Size: 93 5380
BsmBI CGTCTCn'nnnn_
Cuts at: 2277 2277
Size: 5473
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2092 2229 2474 2792 3534 3619 4690
Size: 399 302 137 245 318 742 85 1071
Cuts at: 4690 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 318 302 245 137
85
BspEI T'CCGG_A
Cuts at: 5136 5136
Size: 5473
BspHI T'CATG_A
Cuts at: 2205 3362 5092 2205
Size: 1157 1730 2586
Fragments arranged by size:
2586 1730 1157
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2241 2828 844
Size: 1397 587 3489
Fragments arranged by size:
3489 1397 587
BsrBI CCG'CTC
Cuts at: 1394 1823 3360 1394
Size: 429 1537 3507
Fragments arranged by size:
3507 1537 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3968 4142 65
Size: 1183 2720 174 1396
Fragments arranged by size:
2720 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4117 4304 1700
Size: 110 2307 187 2869
Fragments arranged by size:
2869 2307 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5473
BssHII G'CGCG_C
Cuts at: 1925 2303 1925
Size: 378 5095
BssSI C'ACGA_G
Cuts at: 3533 4545 3533
Size: 1012 4461
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2233 794
Size: 1439 4034
BstBI TT'CG_AA
Cuts at: 4277 4277
Size: 5473
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5473
BstYI r'GATC_y
Cuts at: 1972 2803 3170 3555 3572 5024 5110 5122 5468
Size: 831 367 385 17 1452 86 12 346
Cuts at: 5468 1972
Size: 1977
Fragments arranged by size:
1977 1452 831 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2105 2376 2577 513
Size: 552 1040 271 201 3409
Fragments arranged by size:
3409 1040 552 271 201
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2259 487
Size: 683 56 1033 3701
Fragments arranged by size:
3701 1033 683 56
BtsI GCAGTG_nn'
Cuts at: 2188 2989 3783 3803 2188
Size: 801 794 20 3858
Fragments arranged by size:
3858 801 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5473
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5438
DraI TTT'AAA
Cuts at: 2777 2777
Size: 5473
DraIII CAC_nnn'GTG
Cuts at: 2173 2173
Size: 5473
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4480 809
Size: 507 3164 1802
Fragments arranged by size:
3164 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2920 3809 5438
Size: 54 1425 50 275 18 1090 889 1629
Cuts at: 5438 8
Size: 43
Fragments arranged by size:
1629 1425 1090 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2920 1537
Size: 275 1108 4090
Fragments arranged by size:
4090 1108 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2759 3403 1353
Size: 1406 644 3423
Fragments arranged by size:
3423 1406 644
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4060 4434 4580 387
Size: 1064 138 2471 374 146 1280
Fragments arranged by size:
2471 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3536 4133 4920 5188 1239
Size: 393 1904 597 787 268 1524
Fragments arranged by size:
1904 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2434 2636 2434
Size: 202 5271
EcoRI G'AATT_C
Cuts at: 2782 3160 2782
Size: 378 5095
FspI TGC'GCA
Cuts at: 2237 3979 2237
Size: 1742 3731
HaeII r_GCGC'y
Cuts at: 1678 2151 2566 4620 1678
Size: 473 415 2054 2531
Fragments arranged by size:
2531 2054 473 415
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2257 2289 2628 2660 4114
Size: 32 135 32 685 32 339 32 1454
Cuts at: 4114 4146 4188 4220 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1454 685 339 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2817 3063 669
Size: 988 281 879 246 3079
Fragments arranged by size:
3079 988 879 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5473
HpaI GTT'AAC
Cuts at: 3063 3063
Size: 5473
KasI G'GCGC_C
Cuts at: 2562 2562
Size: 5473
KpnI G_GTAC'C
Cuts at: 2798 2798
Size: 5473
MfeI C'AATT_G
Cuts at: 3072 3072
Size: 5473
MluI A'CGCG_T
Cuts at: 2390 5349 2390
Size: 2959 2514
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4587 4771 1305
Size: 3282 184 2007
Fragments arranged by size:
3282 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3651
Fragments arranged by size:
3651 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2210 2579 3431 3790 3949 518
Size: 648 1044 369 852 359 159 2042
Fragments arranged by size:
2042 1044 852 648 369 359 159
MspA1I CmG'CkG
Cuts at: 1606 2378 2465 2889 3566 4714 4959 1606
Size: 772 87 424 677 1148 245 2120
Fragments arranged by size:
2120 1148 772 677 424 245 87
NarI GG'CG_CC
Cuts at: 2563 2563
Size: 5473
NcoI C'CATG_G
Cuts at: 513 1065 2105 2577 513
Size: 552 1040 472 3409
Fragments arranged by size:
3409 1040 552 472
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5473
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5473
NotI GC'GGCC_GC
Cuts at: 2920 2920
Size: 5473
NspI r_CATG'y
Cuts at: 1132 1229 2831 4376 1132
Size: 97 1602 1545 2229
Fragments arranged by size:
2229 1602 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2558 1348
Size: 1210 4263
PciI A'CATG_T
Cuts at: 4372 4372
Size: 5473
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2740 1108
Size: 1632 3841
PmeI GTTT'AAAC
Cuts at: 2777 2777
Size: 5473
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3542 3574 5055 5087 3542
Size: 32 1481 32 3928
Fragments arranged by size:
3928 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2636 2636
Size: 5473
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5473
PsiI TTA'TAA
Cuts at: 3043 3043
Size: 5473
PstI C_TGCA'G
Cuts at: 830 2664 2825 830
Size: 1834 161 3478
Fragments arranged by size:
3478 1834 161
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5473
SacII CC_GC'GG
Cuts at: 2379 2379
Size: 5473
SalI G'TCGA_C
Cuts at: 1936 2815 1936
Size: 879 4594
SanDI GG'GwC_CC
Cuts at: 2636 2636
Size: 5473
SbfI CC_TGCA'GG
Cuts at: 2664 2825 2664
Size: 161 5312
ScaI AGT'ACT
Cuts at: 3721 5433 3721
Size: 1712 3761
SfcI C'TryA_G
Cuts at: 826 1045 2660 2821 3956 4637 4828 826
Size: 219 1615 161 1135 681 191 1471
Fragments arranged by size:
1615 1471 1135 681 219 191 161
SfoI GGC'GCC
Cuts at: 2564 2564
Size: 5473
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5473
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5473
SmaI CCC'GGG
Cuts at: 1872 2406 2800 1872
Size: 534 394 4545
Fragments arranged by size:
4545 534 394
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3577 4478 4740 820
Size: 197 926 1634 901 262 1553
Fragments arranged by size:
1634 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3264 493
Size: 2771 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5473
SphI G_CATG'C
Cuts at: 1132 2831 1132
Size: 1699 3774
SspI AAT'ATT
Cuts at: 5 52 3397 5
Size: 47 3345 2081
Fragments arranged by size:
3345 2081 47
StuI AGG'CCT
Cuts at: 1517 2414 1517
Size: 897 4576
StyI C'CwwG_G
Cuts at: 513 1065 2105 2577 2912 513
Size: 552 1040 472 335 3074
Fragments arranged by size:
3074 1040 552 472 335
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2161 3500 3517 3670 3855 4300 325
Size: 1255 581 1339 17 153 185 445 1498
Fragments arranged by size:
1498 1339 1255 581 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3719 5431 96
Size: 274 80 33 51 1443 1742 1712 138
Fragments arranged by size:
1742 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5433 5465 5433
Size: 32 5441
Tth111I GACn'n_nGTC
Cuts at: 1316 2269 1316
Size: 953 4520
XbaI T'CTAG_A
Cuts at: 2809 2809
Size: 5473
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2453 2453
Size: 5473
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5473
XmaI C'CCGG_G
Cuts at: 1870 2404 2798 1870
Size: 534 394 4545
Fragments arranged by size:
4545 534 394
XmnI GAAnn'nnTTC
Cuts at: 3602 3602
Size: 5473
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2686 3281 276
Size: 53 83 186 936 1152 595 2468
Fragments arranged by size:
2468 1152 936 595 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SacII SalI SanDI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SapI SexAI SfiI SrfI SwaI