Restriction map for
pFN21AE5249
(Circular) (Six-base) MAPSORT of: pFN21AE5249.seq Check: 5530 from: 1 to: 5251
pFN21AE5249 5251 bp
With 182 enzymes: SgfI *
January 30, 2014 17:15 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3061 278
Size: 53 83 186 936 1525 2468
Fragments arranged by size:
2468 1525 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2594 1937
Size: 657 4594
Acc65I G'GTAC_C
Cuts at: 2572 2572
Size: 5251
AclI AA'CG_TT
Cuts at: 1563 3378 3751 1563
Size: 1815 373 3063
Fragments arranged by size:
3063 1815 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2467 3314 4698 4966 1632
Size: 835 847 1384 268 1917
Fragments arranged by size:
1917 1384 847 835 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5054
AflIII A'CryG_T
Cuts at: 1203 4150 5127 1203
Size: 2947 977 1327
Fragments arranged by size:
2947 1327 977
AgeI A'CCGG_T
Cuts at: 4082 4082
Size: 5251
AhdI GACnn_n'nnGTC
Cuts at: 2043 3980 2043
Size: 1937 3314
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5251
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5251
AlwNI CAG_nnn'CTG
Cuts at: 2994 4566 2994
Size: 1572 3679
ApaLI G'TGCA_C
Cuts at: 3308 4464 3308
Size: 1156 4095
ApoI r'AATT_y
Cuts at: 1493 2560 2786 2938 5047 1493
Size: 1067 226 152 2109 1697
Fragments arranged by size:
2109 1697 1067 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3805 160
Size: 872 2773 1606
Fragments arranged by size:
2773 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5251
AvaI C'yCGr_G
Cuts at: 1870 1943 2114 2576 1870
Size: 73 171 462 4545
Fragments arranged by size:
4545 462 171 73
BamHI G'GATC_C
Cuts at: 2581 2948 2581
Size: 367 4884
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2057 2402 2572 4027 618
Size: 325 217 627 270 345 170 1455 1842
Fragments arranged by size:
1842 1455 627 345 325 270 217 170
BanII G_rGCy'C
Cuts at: 1391 2570 1391
Size: 1179 4072
BbeI G_GCGC'C
Cuts at: 2061 2061
Size: 5251
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2218 928
Size: 971 319 3961
Fragments arranged by size:
3961 971 319
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3442 3476 1903
Size: 34 1505 34 3678
Fragments arranged by size:
3678 1505 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2202 3143 4353 2202
Size: 941 1210 3100
Fragments arranged by size:
3100 1210 941
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5251
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3862 136
Size: 107 122 71 631 605 2190 1525
Fragments arranged by size:
2190 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5246 5246
Size: 5251
BlpI GC'TnA_GC
Cuts at: 2668 2668
Size: 5251
Bme1580I G_kGCm'C
Cuts at: 1790 2405 3312 4468 1790
Size: 615 907 1156 2573
Fragments arranged by size:
2573 1156 907 615
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5251
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2982 3940 442
Size: 416 53 83 399 24 1565 958 1753
Fragments arranged by size:
1753 1565 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5251
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3911 1239
Size: 2672 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3376 4241 4539 3376
Size: 865 298 4088
Fragments arranged by size:
4088 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3914 882
Size: 617 2415 2219
Fragments arranged by size:
2415 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3042 493
Size: 711 1838 2702
Fragments arranged by size:
2702 1838 711
BsaBI GATnn'nnATC
Cuts at: 5245 5245
Size: 5251
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2058 3058 3440 4075
Size: 53 83 186 936 525 1000 382 635
Cuts at: 4075 275
Size: 1451
Fragments arranged by size:
1451 1000 936 635 525 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3684 4082 4356 4503 4914 3684
Size: 398 274 147 411 4021
Fragments arranged by size:
4021 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5026
Fragments arranged by size:
5026 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2517 1186
Size: 1331 3920
BseYI C'CCAG_C
Cuts at: 2143 2205 4454 4950 2143
Size: 62 2249 496 2444
Fragments arranged by size:
2444 2249 496 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2261 1163
Size: 573 525 4153
Fragments arranged by size:
4153 573 525
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2701 3462 4490 1540
Size: 275 186 700 761 1028 2301
Fragments arranged by size:
2301 1028 761 700 275 186
BsiHKAI G_wGCw'C
Cuts at: 3312 3397 4468 3312
Size: 85 1071 4095
Fragments arranged by size:
4095 1071 85
BsmI GAATG_Cn'
Cuts at: 2762 2855 2762
Size: 93 5158
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2405 2570 3312 3397 4468 1391
Size: 399 615 165 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 615 399 165 85
BspEI T'CCGG_A
Cuts at: 4914 4914
Size: 5251
BspHI T'CATG_A
Cuts at: 3140 4870 3140
Size: 1730 3521
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2606 844
Size: 1762 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3138 1394
Size: 429 1315 3507
Fragments arranged by size:
3507 1315 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2521 3746 3920 65
Size: 1183 1273 1225 174 1396
Fragments arranged by size:
1396 1273 1225 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3895 4082 1700
Size: 110 2085 187 2869
Fragments arranged by size:
2869 2085 187 110
BsrGI T'GTAC_A
Cuts at: 96 2289 96
Size: 2193 3058
BssHII G'CGCG_C
Cuts at: 1925 2422 1925
Size: 497 4754
BssSI C'ACGA_G
Cuts at: 3311 4323 3311
Size: 1012 4239
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2140 794
Size: 1346 3905
BstBI TT'CG_AA
Cuts at: 4055 4055
Size: 5251
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5251
BstXI CCAn_nnnn'nTGG
Cuts at: 2533 2533
Size: 5251
BstYI r'GATC_y
Cuts at: 1972 2581 2948 3333 3350 4802 4888 4900 5246
Size: 609 367 385 17 1452 86 12 346
Cuts at: 5246 1972
Size: 1977
Fragments arranged by size:
1977 1452 609 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2396 2396
Size: 5251
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3760
Fragments arranged by size:
3760 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2347 487
Size: 683 56 1121 3391
Fragments arranged by size:
3391 1121 683 56
BtsI GCAGTG_nn'
Cuts at: 2767 3561 3581 2767
Size: 794 20 4437
Fragments arranged by size:
4437 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5251
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5216
DraI TTT'AAA
Cuts at: 2555 2555
Size: 5251
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4258 809
Size: 507 2942 1802
Fragments arranged by size:
2942 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2303 2520
Size: 54 1425 50 275 18 177 296 217
Cuts at: 2520 2698 3587 5216 8
Size: 178 889 1629 43
Fragments arranged by size:
1629 1425 889 296 275 217 178 177
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2698 1537
Size: 275 886 4090
Fragments arranged by size:
4090 886 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2258 3181 1353
Size: 905 923 3423
Fragments arranged by size:
3423 923 905
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3838 4212 4358 387
Size: 1064 138 2249 374 146 1280
Fragments arranged by size:
2249 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2467 3314 3911 4698 4966 1239
Size: 393 835 847 597 787 268 1524
Fragments arranged by size:
1524 847 835 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2560 2938 2560
Size: 378 4873
FspI TGC'GCA
Cuts at: 2052 3757 2052
Size: 1705 3546
HaeII r_GCGC'y
Cuts at: 1678 2061 2081 2123 2165 2337 4398 1678
Size: 383 20 42 42 172 2061 2531
Fragments arranged by size:
2531 2061 383 172 42 42 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2003 2029 2035 2061 2375
Size: 32 135 32 431 26 6 26 314
Cuts at: 2375 2407 3892 3924 3966 3998 1373
Size: 32 1485 32 42 32 2626
Fragments arranged by size:
2626 1485 431 314 135 42 32 32
32 32 32 26 26 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2383 2595 2841 669
Size: 988 281 445 212 246 3079
Fragments arranged by size:
3079 988 445 281 246 212
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5251
HpaI GTT'AAC
Cuts at: 2841 2841
Size: 5251
KasI G'GCGC_C
Cuts at: 2057 2057
Size: 5251
KpnI G_GTAC'C
Cuts at: 2576 2576
Size: 5251
MfeI C'AATT_G
Cuts at: 2850 2850
Size: 5251
MluI A'CGCG_T
Cuts at: 5127 5127
Size: 5251
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4365 4549 1305
Size: 3060 184 2007
Fragments arranged by size:
3060 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2305 10
Size: 54 1425 343 473 2956
Fragments arranged by size:
2956 1425 473 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3209 3568 3727 518
Size: 648 2043 359 159 2042
Fragments arranged by size:
2043 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2137 2275 2667 3344 4492 4737 1606
Size: 531 138 392 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 531 392 245 138
NarI GG'CG_CC
Cuts at: 2058 2058
Size: 5251
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3760
Fragments arranged by size:
3760 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5251
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5251
NotI GC'GGCC_GC
Cuts at: 2698 2698
Size: 5251
NspI r_CATG'y
Cuts at: 1132 1229 2609 4154 1132
Size: 97 1380 1545 2229
Fragments arranged by size:
2229 1545 1380 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5251
PciI A'CATG_T
Cuts at: 4150 4150
Size: 5251
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5251
PmeI GTTT'AAAC
Cuts at: 2555 2555
Size: 5251
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3320 3352 4833 4865 3320
Size: 32 1481 32 3706
Fragments arranged by size:
3706 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2246 1613
Size: 633 4618
PsiI TTA'TAA
Cuts at: 2415 2821 2415
Size: 406 4845
PstI C_TGCA'G
Cuts at: 830 2136 2603 830
Size: 1306 467 3478
Fragments arranged by size:
3478 1306 467
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5251
PvuII CAG'CTG
Cuts at: 2137 2275 2137
Size: 138 5113
SalI G'TCGA_C
Cuts at: 1936 2593 1936
Size: 657 4594
SbfI CC_TGCA'GG
Cuts at: 2603 2603
Size: 5251
ScaI AGT'ACT
Cuts at: 3499 5211 3499
Size: 1712 3539
SfcI C'TryA_G
Cuts at: 826 1045 2132 2599 3734 4415 4606 826
Size: 219 1087 467 1135 681 191 1471
Fragments arranged by size:
1471 1135 1087 681 467 219 191
SfoI GGC'GCC
Cuts at: 2059 2059
Size: 5251
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5251
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5251
SmaI CCC'GGG
Cuts at: 1872 2578 1872
Size: 706 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3355 4256 4518 820
Size: 197 926 1412 901 262 1553
Fragments arranged by size:
1553 1412 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3042 493
Size: 2549 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5251
SphI G_CATG'C
Cuts at: 1132 2609 1132
Size: 1477 3774
SspI AAT'ATT
Cuts at: 5 52 3175 5
Size: 47 3123 2081
Fragments arranged by size:
3123 2081 47
StuI AGG'CCT
Cuts at: 1517 2495 1517
Size: 978 4273
StyI C'CwwG_G
Cuts at: 513 1065 2004 2690 513
Size: 552 939 686 3074
Fragments arranged by size:
3074 939 686 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2197 3278 3295 3448 3633 4078 325
Size: 1255 617 1081 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1081 617 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2289 3497 5209
Size: 274 80 33 51 1443 312 1208 1712
Cuts at: 5209 96
Size: 138
Fragments arranged by size:
1712 1443 1208 312 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5211 5243 5211
Size: 32 5219
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5251
XbaI T'CTAG_A
Cuts at: 2587 2587
Size: 5251
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2530 2530
Size: 5251
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5251
XmaI C'CCGG_G
Cuts at: 1870 2576 1870
Size: 706 4545
XmnI GAAnn'nnTTC
Cuts at: 3380 3380
Size: 5251
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3059 276
Size: 53 83 186 936 1525 2468
Fragments arranged by size:
2468 1525 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SrfI SwaI