Restriction map for
pFN21AE5246
(Circular) (Six-base) MAPSORT of: pFN21AE5246.seq Check: 299 from: 1 to: 5434
pFN21AE5246 5434 bp
With 182 enzymes: SgfI *
January 30, 2014 17:15 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3244 278
Size: 53 83 186 936 1708 2468
Fragments arranged by size:
2468 1708 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2777 1937
Size: 840 4594
Acc65I G'GTAC_C
Cuts at: 2755 2755
Size: 5434
AclI AA'CG_TT
Cuts at: 1563 3561 3934 1563
Size: 1998 373 3063
Fragments arranged by size:
3063 1998 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2453 3497 4881 5149 1632
Size: 821 1044 1384 268 1917
Fragments arranged by size:
1917 1384 1044 821 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5237
AflIII A'CryG_T
Cuts at: 1203 4333 5310 1203
Size: 3130 977 1327
Fragments arranged by size:
3130 1327 977
AgeI A'CCGG_T
Cuts at: 4265 4265
Size: 5434
AhdI GACnn_n'nnGTC
Cuts at: 4163 4163
Size: 5434
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5434
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5434
AlwNI CAG_nnn'CTG
Cuts at: 3177 4749 3177
Size: 1572 3862
ApaLI G'TGCA_C
Cuts at: 3491 4647 3491
Size: 1156 4278
ApoI r'AATT_y
Cuts at: 1493 2453 2743 2969 3121 5230 1493
Size: 960 290 226 152 2109 1697
Fragments arranged by size:
2109 1697 960 290 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3988 160
Size: 872 2956 1606
Fragments arranged by size:
2956 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5434
AvaI C'yCGr_G
Cuts at: 1870 1943 2759 1870
Size: 73 816 4545
Fragments arranged by size:
4545 816 73
BamHI G'GATC_C
Cuts at: 2764 3131 2764
Size: 367 5067
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2755 4210 618
Size: 325 217 627 968 1455 1842
Fragments arranged by size:
1842 1455 968 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2338 2753 1391
Size: 947 415 4072
Fragments arranged by size:
4072 947 415
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4463
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3625 3659 1903
Size: 34 1688 34 3678
Fragments arranged by size:
3678 1688 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2565 3326 4536 2565
Size: 761 1210 3463
Fragments arranged by size:
3463 1210 761
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5434
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2053 4045 136
Size: 107 122 71 631 605 381 1992 1525
Fragments arranged by size:
1992 1525 631 605 381 122 107 71
BglII A'GATC_T
Cuts at: 5429 5429
Size: 5434
BlpI GC'TnA_GC
Cuts at: 2851 2851
Size: 5434
Bme1580I G_kGCm'C
Cuts at: 1790 3495 4651 1790
Size: 1705 1156 2573
Fragments arranged by size:
2573 1705 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5434
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3165 4123 442
Size: 416 53 83 399 24 1748 958 1753
Fragments arranged by size:
1753 1748 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5434
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2249 4094 1239
Size: 1010 1845 2579
Fragments arranged by size:
2579 1845 1010
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2114 2498 3559 4424 4722 2114
Size: 384 1061 865 298 2826
Fragments arranged by size:
2826 1061 865 384 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2225 4097 882
Size: 617 726 1872 2219
Fragments arranged by size:
2219 1872 726 617
BsaAI yAC'GTr
Cuts at: 493 1204 3225 493
Size: 711 2021 2702
Fragments arranged by size:
2702 2021 711
BsaBI GATnn'nnATC
Cuts at: 2078 2292 5428 2078
Size: 214 3136 2084
Fragments arranged by size:
3136 2084 214
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3241 3623 4258 275
Size: 53 83 186 936 1708 382 635 1451
Fragments arranged by size:
1708 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3867 4265 4539 4686 5097 3867
Size: 398 274 147 411 4204
Fragments arranged by size:
4204 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5209
Fragments arranged by size:
5209 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2525 1186
Size: 1339 4095
BseYI C'CCAG_C
Cuts at: 4637 5133 4637
Size: 496 4938
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4861
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2884 3645 4673 1540
Size: 275 186 883 761 1028 2301
Fragments arranged by size:
2301 1028 883 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2338 2650 3495 3580 4651 2338
Size: 312 845 85 1071 3121
Fragments arranged by size:
3121 1071 845 312 85
BsmI GAATG_Cn'
Cuts at: 2426 2945 3038 2426
Size: 519 93 4822
Fragments arranged by size:
4822 519 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2338 2650 2753 3495 3580 4651 1391
Size: 399 548 312 103 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 548 399 312 103 85
BspEI T'CCGG_A
Cuts at: 5097 5097
Size: 5434
BspHI T'CATG_A
Cuts at: 3323 5053 3323
Size: 1730 3704
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2789 844
Size: 1945 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2554 3321 1394
Size: 429 731 767 3507
Fragments arranged by size:
3507 767 731 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3929 4103 65
Size: 1183 2681 174 1396
Fragments arranged by size:
2681 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4078 4265 1700
Size: 110 2268 187 2869
Fragments arranged by size:
2869 2268 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5434
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5434
BssSI C'ACGA_G
Cuts at: 3494 4506 3494
Size: 1012 4422
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5434
BstBI TT'CG_AA
Cuts at: 4238 4238
Size: 5434
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5434
BstXI CCAn_nnnn'nTGG
Cuts at: 2029 2029
Size: 5434
BstYI r'GATC_y
Cuts at: 1972 2764 3131 3516 3533 4985 5071 5083 5429
Size: 792 367 385 17 1452 86 12 346
Cuts at: 5429 1972
Size: 1977
Fragments arranged by size:
1977 1452 792 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4882
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4695
Fragments arranged by size:
4695 683 56
BtsI GCAGTG_nn'
Cuts at: 2950 3744 3764 2950
Size: 794 20 4620
Fragments arranged by size:
4620 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5434
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2482 2517 529
Size: 35 1918 35 3446
Fragments arranged by size:
3446 1918 35 35
DraI TTT'AAA
Cuts at: 2738 2738
Size: 5434
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2639 4441 809
Size: 507 1323 1802 1802
Fragments arranged by size:
1802 1802 1323 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2212 2881 3770
Size: 54 1425 50 275 18 382 669 889
Cuts at: 3770 5399 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 669 382 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2881 1537
Size: 275 1069 4090
Fragments arranged by size:
4090 1069 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2463 2655 3364 1353
Size: 1110 192 709 3423
Fragments arranged by size:
3423 1110 709 192
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4021 4395 4541 387
Size: 1064 138 2432 374 146 1280
Fragments arranged by size:
2432 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2336 2336
Size: 5434
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2249 2453 3497 4094 4881 5149 1239
Size: 393 617 204 1044 597 787 268 1524
Fragments arranged by size:
1524 1044 787 617 597 393 268 204
EcoNI CCTnn'n_nnAGG
Cuts at: 2447 2447
Size: 5434
EcoO109I rG'GnC_Cy
Cuts at: 2025 2115 2371 2632 2025
Size: 90 256 261 4827
Fragments arranged by size:
4827 261 256 90
EcoRI G'AATT_C
Cuts at: 2453 2743 3121 2453
Size: 290 378 4766
Fragments arranged by size:
4766 378 290
EcoRV GAT'ATC
Cuts at: 2616 2616
Size: 5434
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2492 2492
Size: 5434
FspI TGC'GCA
Cuts at: 3940 3940
Size: 5434
HaeII r_GCGC'y
Cuts at: 1678 4581 1678
Size: 2903 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4075 4107 4149 4181 1373
Size: 32 135 32 2503 32 42 32 2626
Fragments arranged by size:
2626 2503 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2161 2246 2778 3024 669
Size: 988 281 223 85 532 246 3079
Fragments arranged by size:
3079 988 532 281 246 223 85
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5434
HpaI GTT'AAC
Cuts at: 3024 3024
Size: 5434
KpnI G_GTAC'C
Cuts at: 2759 2759
Size: 5434
MfeI C'AATT_G
Cuts at: 3033 3033
Size: 5434
MluI A'CGCG_T
Cuts at: 5310 5310
Size: 5434
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4548 4732 1305
Size: 3243 184 2007
Fragments arranged by size:
3243 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2214 10
Size: 54 1425 343 382 3230
Fragments arranged by size:
3230 1425 382 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3392 3751 3910 518
Size: 648 2226 359 159 2042
Fragments arranged by size:
2226 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2850 3527 4675 4920 1606
Size: 1244 677 1148 245 2120
Fragments arranged by size:
2120 1244 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4882
NdeI CA'TA_TG
Cuts at: 387 2317 387
Size: 1930 3504
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5434
NotI GC'GGCC_GC
Cuts at: 2881 2881
Size: 5434
NspI r_CATG'y
Cuts at: 1132 1229 2792 4337 1132
Size: 97 1563 1545 2229
Fragments arranged by size:
2229 1563 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2029 2051 1348
Size: 681 22 4731
Fragments arranged by size:
4731 681 22
PciI A'CATG_T
Cuts at: 4333 4333
Size: 5434
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2628 1108
Size: 1520 3914
PfoI T'CCnGG_A
Cuts at: 2061 2226 2296 2061
Size: 165 70 5199
Fragments arranged by size:
5199 165 70
PmeI GTTT'AAAC
Cuts at: 2738 2738
Size: 5434
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3503 3535 5016 5048 3503
Size: 32 1481 32 3889
Fragments arranged by size:
3889 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2025 2115 2371 2632 2025
Size: 90 256 261 4827
Fragments arranged by size:
4827 261 256 90
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5434
PsiI TTA'TAA
Cuts at: 3004 3004
Size: 5434
PstI C_TGCA'G
Cuts at: 830 2786 830
Size: 1956 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5434
SacI G_AGCT'C
Cuts at: 2338 2338
Size: 5434
SalI G'TCGA_C
Cuts at: 1936 2776 1936
Size: 840 4594
SanDI GG'GwC_CC
Cuts at: 2632 2632
Size: 5434
SapI GCTCTTCn'nnn_
Cuts at: 2655 2655
Size: 5434
SbfI CC_TGCA'GG
Cuts at: 2786 2786
Size: 5434
ScaI AGT'ACT
Cuts at: 3682 5394 3682
Size: 1712 3722
SfcI C'TryA_G
Cuts at: 826 1045 2083 2782 3917 4598 4789 826
Size: 219 1038 699 1135 681 191 1471
Fragments arranged by size:
1471 1135 1038 699 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5434
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5434
SmaI CCC'GGG
Cuts at: 1872 2761 1872
Size: 889 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2129 2477 3538 4439 4701 820
Size: 197 926 186 348 1061 901 262 1553
Fragments arranged by size:
1553 1061 926 901 348 262 197 186
SnaBI TAC'GTA
Cuts at: 493 3225 493
Size: 2732 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5434
SphI G_CATG'C
Cuts at: 1132 2792 1132
Size: 1660 3774
SspI AAT'ATT
Cuts at: 5 52 3358 5
Size: 47 3306 2081
Fragments arranged by size:
3306 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5434
StyI C'CwwG_G
Cuts at: 513 1065 2385 2873 513
Size: 552 1320 488 3074
Fragments arranged by size:
3074 1320 552 488
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3461 3478 3631 3816 4261 325
Size: 1255 1881 17 153 185 445 1498
Fragments arranged by size:
1881 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3680 5392 96
Size: 274 80 33 51 1443 1703 1712 138
Fragments arranged by size:
1712 1703 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5394 5426 5394
Size: 32 5402
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5434
XbaI T'CTAG_A
Cuts at: 2770 2770
Size: 5434
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2392 2392
Size: 5434
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5434
XmaI C'CCGG_G
Cuts at: 1870 2759 1870
Size: 889 4545
XmnI GAAnn'nnTTC
Cuts at: 2472 3563 2472
Size: 1091 4343
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3242 276
Size: 53 83 186 936 1708 2468
Fragments arranged by size:
2468 1708 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SacI SalI SanDI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII
SacII SexAI SfiI SfoI SrfI SwaI