Restriction map for
pFN21AE5236
(Circular) (Six-base) MAPSORT of: pFN21AE5236.seq Check: 435 from: 1 to: 5458
pFN21AE5236 5458 bp
With 182 enzymes: SgfI *
January 30, 2014 17:15 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3268 278
Size: 53 83 186 936 1732 2468
Fragments arranged by size:
2468 1732 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2801 1937
Size: 864 4594
Acc65I G'GTAC_C
Cuts at: 2779 2779
Size: 5458
AclI AA'CG_TT
Cuts at: 1563 3585 3958 1563
Size: 2022 373 3063
Fragments arranged by size:
3063 2022 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2256 3521 4905 5173 1632
Size: 624 1265 1384 268 1917
Fragments arranged by size:
1917 1384 1265 624 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5261
AflIII A'CryG_T
Cuts at: 1203 2355 2752 4357 5334 1203
Size: 1152 397 1605 977 1327
Fragments arranged by size:
1605 1327 1152 977 397
AgeI A'CCGG_T
Cuts at: 4289 4289
Size: 5458
AhdI GACnn_n'nnGTC
Cuts at: 4187 4187
Size: 5458
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5458
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5458
AlwNI CAG_nnn'CTG
Cuts at: 3201 4773 3201
Size: 1572 3886
ApaLI G'TGCA_C
Cuts at: 3515 4671 3515
Size: 1156 4302
ApoI r'AATT_y
Cuts at: 1493 2206 2507 2767 2993 3145 5254 1493
Size: 713 301 260 226 152 2109 1697
Fragments arranged by size:
2109 1697 713 301 260 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4012 160
Size: 872 2980 1606
Fragments arranged by size:
2980 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5458
AvaI C'yCGr_G
Cuts at: 1870 1943 2783 1870
Size: 73 840 4545
Fragments arranged by size:
4545 840 73
BamHI G'GATC_C
Cuts at: 2788 3155 2788
Size: 367 5091
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2047 2170 2779 4234 618
Size: 325 217 627 260 123 609 1455 1842
Fragments arranged by size:
1842 1455 627 609 325 260 217 123
BanII G_rGCy'C
Cuts at: 1391 2699 2777 1391
Size: 1308 78 4072
Fragments arranged by size:
4072 1308 78
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4487
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3649 3683 1903
Size: 34 1712 34 3678
Fragments arranged by size:
3678 1712 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3350 4560 3350
Size: 1210 4248
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5458
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4069 136
Size: 107 122 71 631 605 2397 1525
Fragments arranged by size:
2397 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5453 5453
Size: 5458
BlpI GC'TnA_GC
Cuts at: 2875 2875
Size: 5458
Bme1580I G_kGCm'C
Cuts at: 1790 2052 2071 2173 3519 4675 1790
Size: 262 19 102 1346 1156 2573
Fragments arranged by size:
2573 1346 1156 262 102 19
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5458
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2102 2737 3189
Size: 416 53 83 399 24 685 635 452
Cuts at: 3189 4147 442
Size: 958 1753
Fragments arranged by size:
1753 958 685 635 452 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5458
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4118 1239
Size: 2879 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3583 4448 4746 3583
Size: 865 298 4295
Fragments arranged by size:
4295 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4121 882
Size: 617 2622 2219
Fragments arranged by size:
2622 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2455 3249 493
Size: 711 1251 794 2702
Fragments arranged by size:
2702 1251 794 711
BsaBI GATnn'nnATC
Cuts at: 5452 5452
Size: 5458
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3265 3647 4282 275
Size: 53 83 186 936 1732 382 635 1451
Fragments arranged by size:
1732 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3891 4289 4563 4710 5121 3891
Size: 398 274 147 411 4228
Fragments arranged by size:
4228 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5233
Fragments arranged by size:
5233 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2063 1186
Size: 877 4581
BseYI C'CCAG_C
Cuts at: 2674 4661 5157 2674
Size: 1987 496 2975
Fragments arranged by size:
2975 1987 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4885
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2908 3669 4697 1540
Size: 275 186 907 761 1028 2301
Fragments arranged by size:
2301 1028 907 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3519 3604 4675 3519
Size: 85 1071 4302
Fragments arranged by size:
4302 1071 85
BsmI GAATG_Cn'
Cuts at: 2969 3062 2969
Size: 93 5365
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2071 2173 2699 2777 3519 3604
Size: 399 262 19 102 526 78 742 85
Cuts at: 3604 4675 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 526 399 262 102 85
78 19
BspEI T'CCGG_A
Cuts at: 5121 5121
Size: 5458
BspHI T'CATG_A
Cuts at: 3347 5077 3347
Size: 1730 3728
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2301 2813 844
Size: 1457 512 3489
Fragments arranged by size:
3489 1457 512
BsrBI CCG'CTC
Cuts at: 1394 1823 2038 2337 3345 1394
Size: 429 215 299 1008 3507
Fragments arranged by size:
3507 1008 429 299 215
BsrDI GCAATG_nn'
Cuts at: 65 1248 3953 4127 65
Size: 1183 2705 174 1396
Fragments arranged by size:
2705 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4102 4289 1700
Size: 110 2292 187 2869
Fragments arranged by size:
2869 2292 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5458
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5458
BssSI C'ACGA_G
Cuts at: 3518 4530 3518
Size: 1012 4446
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5458
BstBI TT'CG_AA
Cuts at: 4262 4262
Size: 5458
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5458
BstXI CCAn_nnnn'nTGG
Cuts at: 2674 2755 2674
Size: 81 5377
BstYI r'GATC_y
Cuts at: 1972 2788 3155 3540 3557 5009 5095 5107 5453
Size: 816 367 385 17 1452 86 12 346
Cuts at: 5453 1972
Size: 1977
Fragments arranged by size:
1977 1452 816 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2578 513
Size: 552 1513 3393
Fragments arranged by size:
3393 1513 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2402 487
Size: 683 56 1176 3543
Fragments arranged by size:
3543 1176 683 56
BtsI GCAGTG_nn'
Cuts at: 2099 2661 2974 3768 3788 2099
Size: 562 313 794 20 3769
Fragments arranged by size:
3769 794 562 313 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5458
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5423
DraI TTT'AAA
Cuts at: 2762 2762
Size: 5458
DraIII CAC_nnn'GTG
Cuts at: 2455 2455
Size: 5458
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4465 809
Size: 507 3149 1802
Fragments arranged by size:
3149 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2473 2905 3794
Size: 54 1425 50 275 18 643 432 889
Cuts at: 3794 5423 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 643 432 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2905 1537
Size: 275 1093 4090
Fragments arranged by size:
4090 1093 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2188 3388 1353
Size: 835 1200 3423
Fragments arranged by size:
3423 1200 835
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4045 4419 4565 387
Size: 1064 138 2456 374 146 1280
Fragments arranged by size:
2456 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2256 3521 4118 4905 5173 1239
Size: 393 624 1265 597 787 268 1524
Fragments arranged by size:
1524 1265 787 624 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2534 2534
Size: 5458
EcoO109I rG'GnC_Cy
Cuts at: 2225 2225
Size: 5458
EcoRI G'AATT_C
Cuts at: 2767 3145 2767
Size: 378 5080
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2546 2572 2546
Size: 26 5432
FspI TGC'GCA
Cuts at: 3964 3964
Size: 5458
HaeII r_GCGC'y
Cuts at: 1678 4605 1678
Size: 2927 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4099 4131 4173 4205 1373
Size: 32 135 32 2527 32 42 32 2626
Fragments arranged by size:
2626 2527 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2802 3048 669
Size: 988 281 864 246 3079
Fragments arranged by size:
3079 988 864 281 246
HindIII A'AGCT_T
Cuts at: 748 2150 748
Size: 1402 4056
HpaI GTT'AAC
Cuts at: 3048 3048
Size: 5458
KpnI G_GTAC'C
Cuts at: 2783 2783
Size: 5458
MfeI C'AATT_G
Cuts at: 3057 3057
Size: 5458
MluI A'CGCG_T
Cuts at: 5334 5334
Size: 5458
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4572 4756 1305
Size: 3267 184 2007
Fragments arranged by size:
3267 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3636
Fragments arranged by size:
3636 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3416 3775 3934 518
Size: 648 2250 359 159 2042
Fragments arranged by size:
2250 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2580 2874 3551 4699 4944 1606
Size: 974 294 677 1148 245 2120
Fragments arranged by size:
2120 1148 974 677 294 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4906
NdeI CA'TA_TG
Cuts at: 387 2366 2701 387
Size: 1979 335 3144
Fragments arranged by size:
3144 1979 335
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5458
NotI GC'GGCC_GC
Cuts at: 2905 2905
Size: 5458
NspI r_CATG'y
Cuts at: 1132 1229 2359 2674 2746 2816 4361 1132
Size: 97 1130 315 72 70 1545 2229
Fragments arranged by size:
2229 1545 1130 315 97 72 70
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5458
PciI A'CATG_T
Cuts at: 2355 4357 2355
Size: 2002 3456
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5458
PmeI GTTT'AAAC
Cuts at: 2762 2762
Size: 5458
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3527 3559 5040 5072 3527
Size: 32 1481 32 3913
Fragments arranged by size:
3913 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2225 2225
Size: 5458
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5458
PsiI TTA'TAA
Cuts at: 3028 3028
Size: 5458
PstI C_TGCA'G
Cuts at: 830 2810 830
Size: 1980 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5458
SacII CC_GC'GG
Cuts at: 2581 2581
Size: 5458
SalI G'TCGA_C
Cuts at: 1936 2800 1936
Size: 864 4594
SbfI CC_TGCA'GG
Cuts at: 2810 2810
Size: 5458
ScaI AGT'ACT
Cuts at: 2691 3706 5418 2691
Size: 1015 1712 2731
Fragments arranged by size:
2731 1712 1015
SexAI A'CCwGG_T
Cuts at: 2535 2535
Size: 5458
SfcI C'TryA_G
Cuts at: 826 1045 2806 3941 4622 4813 826
Size: 219 1761 1135 681 191 1471
Fragments arranged by size:
1761 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5458
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5458
SmaI CCC'GGG
Cuts at: 1872 2785 1872
Size: 913 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3562 4463 4725 820
Size: 197 926 1619 901 262 1553
Fragments arranged by size:
1619 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3249 493
Size: 2756 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5458
SphI G_CATG'C
Cuts at: 1132 2816 1132
Size: 1684 3774
SspI AAT'ATT
Cuts at: 5 52 3382 5
Size: 47 3330 2081
Fragments arranged by size:
3330 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5458
StyI C'CwwG_G
Cuts at: 513 1065 2897 513
Size: 552 1832 3074
Fragments arranged by size:
3074 1832 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2270 2483 3485 3502 3655 3840 4285
Size: 1255 690 213 1002 17 153 185 445
Cuts at: 4285 325
Size: 1498
Fragments arranged by size:
1498 1255 1002 690 445 213 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2689 3704 5416
Size: 274 80 33 51 1443 712 1015 1712
Cuts at: 5416 96
Size: 138
Fragments arranged by size:
1712 1443 1015 712 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5418 5450 5418
Size: 32 5426
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5458
XbaI T'CTAG_A
Cuts at: 2587 2794 2587
Size: 207 5251
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5458
XmaI C'CCGG_G
Cuts at: 1870 2783 1870
Size: 913 4545
XmnI GAAnn'nnTTC
Cuts at: 3587 3587
Size: 5458
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3266 276
Size: 53 83 186 936 1732 2468
Fragments arranged by size:
2468 1732 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SacII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SanDI SapI SfiI SfoI SrfI
SwaI XcmI