Restriction map for
pFN21AE5234
(Circular) (Six-base) MAPSORT of: pFN21AE5234.seq Check: 9362 from: 1 to: 5338
pFN21AE5234 5338 bp
With 182 enzymes: SgfI *
January 30, 2014 17:15 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3148 278
Size: 53 83 186 936 1612 2468
Fragments arranged by size:
2468 1612 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2681 1937
Size: 744 4594
Acc65I G'GTAC_C
Cuts at: 2659 2659
Size: 5338
AclI AA'CG_TT
Cuts at: 1563 3465 3838 1563
Size: 1902 373 3063
Fragments arranged by size:
3063 1902 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3401 4785 5053 1632
Size: 1769 1384 268 1917
Fragments arranged by size:
1917 1769 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5141
AflIII A'CryG_T
Cuts at: 1203 4237 5214 1203
Size: 3034 977 1327
Fragments arranged by size:
3034 1327 977
AgeI A'CCGG_T
Cuts at: 4169 4169
Size: 5338
AhdI GACnn_n'nnGTC
Cuts at: 4067 4067
Size: 5338
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5338
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5338
AlwNI CAG_nnn'CTG
Cuts at: 2599 2629 3081 4653 2599
Size: 30 452 1572 3284
Fragments arranged by size:
3284 1572 452 30
ApaLI G'TGCA_C
Cuts at: 3395 4551 3395
Size: 1156 4182
ApoI r'AATT_y
Cuts at: 1493 2647 2873 3025 5134 1493
Size: 1154 226 152 2109 1697
Fragments arranged by size:
2109 1697 1154 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3892 160
Size: 872 2860 1606
Fragments arranged by size:
2860 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5338
AvaI C'yCGr_G
Cuts at: 1870 1943 2088 2312 2663 1870
Size: 73 145 224 351 4545
Fragments arranged by size:
4545 351 224 145 73
BamHI G'GATC_C
Cuts at: 2668 3035 2668
Size: 367 4971
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2659 4114 618
Size: 325 217 627 872 1455 1842
Fragments arranged by size:
1842 1455 872 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2657 1391
Size: 1266 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2131 928
Size: 971 232 4135
Fragments arranged by size:
4135 971 232
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3529 3563 1903
Size: 34 1592 34 3678
Fragments arranged by size:
3678 1592 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3230 4440 3230
Size: 1210 4128
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5338
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2262 3949 136
Size: 107 122 71 631 605 590 1687 1525
Fragments arranged by size:
1687 1525 631 605 590 122 107 71
BglII A'GATC_T
Cuts at: 5333 5333
Size: 5338
BlpI GC'TnA_GC
Cuts at: 2426 2755 2426
Size: 329 5009
Bme1580I G_kGCm'C
Cuts at: 1790 2012 3399 4555 1790
Size: 222 1387 1156 2573
Fragments arranged by size:
2573 1387 1156 222
BmgBI CAC'GTC
Cuts at: 1318 2275 1318
Size: 957 4381
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3069 4027 442
Size: 416 53 83 399 24 1652 958 1753
Fragments arranged by size:
1753 1652 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5338
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2120 3998 1239
Size: 881 1878 2579
Fragments arranged by size:
2579 1878 881
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2053 3463 4328 4626 2053
Size: 1410 865 298 2765
Fragments arranged by size:
2765 1410 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2079 2214 4001 882
Size: 617 580 135 1787 2219
Fragments arranged by size:
2219 1787 617 580 135
BsaAI yAC'GTr
Cuts at: 493 1204 3129 493
Size: 711 1925 2702
Fragments arranged by size:
2702 1925 711
BsaBI GATnn'nnATC
Cuts at: 5332 5332
Size: 5338
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3145 3527 4162 275
Size: 53 83 186 936 1612 382 635 1451
Fragments arranged by size:
1612 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3771 4169 4443 4590 5001 3771
Size: 398 274 147 411 4108
Fragments arranged by size:
4108 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5113
Fragments arranged by size:
5113 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2117 2147 1186
Size: 931 30 4377
Fragments arranged by size:
4377 931 30
BseYI C'CCAG_C
Cuts at: 2329 4541 5037 2329
Size: 2212 496 2630
Fragments arranged by size:
2630 2212 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4765
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2788 3549 4577 1540
Size: 275 186 787 761 1028 2301
Fragments arranged by size:
2301 1028 787 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3399 3484 4555 3399
Size: 85 1071 4182
Fragments arranged by size:
4182 1071 85
BsmI GAATG_Cn'
Cuts at: 2849 2942 2849
Size: 93 5245
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2657 3399 3484 4555 1391
Size: 399 222 645 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 645 399 222 85
BspEI T'CCGG_A
Cuts at: 5001 5001
Size: 5338
BspHI T'CATG_A
Cuts at: 3227 4957 3227
Size: 1730 3608
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2693 844
Size: 1849 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2152 3225 1394
Size: 429 329 1073 3507
Fragments arranged by size:
3507 1073 429 329
BsrDI GCAATG_nn'
Cuts at: 65 1248 3833 4007 65
Size: 1183 2585 174 1396
Fragments arranged by size:
2585 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2295 3982 4169 1700
Size: 110 485 1687 187 2869
Fragments arranged by size:
2869 1687 485 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5338
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5338
BssSI C'ACGA_G
Cuts at: 2528 3398 4410 2528
Size: 870 1012 3456
Fragments arranged by size:
3456 1012 870
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5338
BstBI TT'CG_AA
Cuts at: 2083 4142 2083
Size: 2059 3279
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5338
BstYI r'GATC_y
Cuts at: 1972 2445 2668 3035 3420 3437 4889 4975 4987
Size: 473 223 367 385 17 1452 86 12
Cuts at: 4987 5333 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 473 385 367 346 223 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2588 2588
Size: 5338
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4786
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4599
Fragments arranged by size:
4599 683 56
BtsI GCAGTG_nn'
Cuts at: 2854 3648 3668 2854
Size: 794 20 4524
Fragments arranged by size:
4524 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5338
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5303
DraI TTT'AAA
Cuts at: 2642 2642
Size: 5338
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4345 809
Size: 507 3029 1802
Fragments arranged by size:
3029 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2201 2785 3674
Size: 54 1425 50 275 18 371 584 889
Cuts at: 3674 5303 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 584 371 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2785 1537
Size: 275 973 4090
Fragments arranged by size:
4090 973 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3268 1353
Size: 1915 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2470 3925 4299 4445 387
Size: 1064 138 881 1455 374 146 1280
Fragments arranged by size:
1455 1280 1064 881 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2120 3401 3998 4785 5053 1239
Size: 393 488 1281 597 787 268 1524
Fragments arranged by size:
1524 1281 787 597 488 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2531 2531
Size: 5338
EcoO109I rG'GnC_Cy
Cuts at: 2094 2094
Size: 5338
EcoRI G'AATT_C
Cuts at: 2647 3025 2647
Size: 378 4960
FspI TGC'GCA
Cuts at: 3844 3844
Size: 5338
HaeII r_GCGC'y
Cuts at: 1678 2426 4485 1678
Size: 748 2059 2531
Fragments arranged by size:
2531 2059 748
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2430 2462 2553 2585 3979
Size: 32 135 32 858 32 91 32 1394
Cuts at: 3979 4011 4053 4085 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1394 858 135 91 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2682 2928 669
Size: 988 281 744 246 3079
Fragments arranged by size:
3079 988 744 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5338
HpaI GTT'AAC
Cuts at: 2928 2928
Size: 5338
KpnI G_GTAC'C
Cuts at: 2663 2663
Size: 5338
MfeI C'AATT_G
Cuts at: 2937 2937
Size: 5338
MluI A'CGCG_T
Cuts at: 5214 5214
Size: 5338
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2127 4452 4636 1305
Size: 822 2325 184 2007
Fragments arranged by size:
2325 2007 822 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2203 10
Size: 54 1425 343 371 3145
Fragments arranged by size:
3145 1425 371 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3296 3655 3814 518
Size: 648 2130 359 159 2042
Fragments arranged by size:
2130 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2754 3431 4579 4824 1606
Size: 1148 677 1148 245 2120
Fragments arranged by size:
2120 1148 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4786
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5338
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5338
NotI GC'GGCC_GC
Cuts at: 2785 2785
Size: 5338
NspI r_CATG'y
Cuts at: 1132 1229 2696 4241 1132
Size: 97 1467 1545 2229
Fragments arranged by size:
2229 1545 1467 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5338
PciI A'CATG_T
Cuts at: 4237 4237
Size: 5338
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2230 1108
Size: 1122 4216
PfoI T'CCnGG_A
Cuts at: 2057 2057
Size: 5338
PmeI GTTT'AAAC
Cuts at: 2642 2642
Size: 5338
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3407 3439 4920 4952 3407
Size: 32 1481 32 3793
Fragments arranged by size:
3793 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2094 2094
Size: 5338
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5338
PsiI TTA'TAA
Cuts at: 2908 2908
Size: 5338
PspXI vC'TCGA_Gb
Cuts at: 2088 2088
Size: 5338
PstI C_TGCA'G
Cuts at: 830 2690 830
Size: 1860 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5338
SalI G'TCGA_C
Cuts at: 1936 2680 1936
Size: 744 4594
SanDI GG'GwC_CC
Cuts at: 2094 2094
Size: 5338
SbfI CC_TGCA'GG
Cuts at: 2690 2690
Size: 5338
ScaI AGT'ACT
Cuts at: 3586 5298 3586
Size: 1712 3626
SfcI C'TryA_G
Cuts at: 826 1045 2686 3821 4502 4693 826
Size: 219 1641 1135 681 191 1471
Fragments arranged by size:
1641 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5338
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5338
SmaI CCC'GGG
Cuts at: 1872 2665 1872
Size: 793 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2068 2088 3442 4343 4605 820
Size: 197 926 125 20 1354 901 262 1553
Fragments arranged by size:
1553 1354 926 901 262 197 125 20
SnaBI TAC'GTA
Cuts at: 493 3129 493
Size: 2636 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5338
SphI G_CATG'C
Cuts at: 1132 2696 1132
Size: 1564 3774
SspI AAT'ATT
Cuts at: 5 52 3262 5
Size: 47 3210 2081
Fragments arranged by size:
3210 2081 47
StuI AGG'CCT
Cuts at: 1517 2208 2318 1517
Size: 691 110 4537
Fragments arranged by size:
4537 691 110
StyI C'CwwG_G
Cuts at: 513 1065 2777 513
Size: 552 1712 3074
Fragments arranged by size:
3074 1712 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2336 3365 3382 3535 3720 4165 325
Size: 1255 756 1029 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1029 756 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3584 5296 96
Size: 274 80 33 51 1443 1607 1712 138
Fragments arranged by size:
1712 1607 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5298 5330 5298
Size: 32 5306
Tth111I GACn'n_nGTC
Cuts at: 1316 2195 1316
Size: 879 4459
XbaI T'CTAG_A
Cuts at: 2674 2674
Size: 5338
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2491 2491
Size: 5338
XhoI C'TCGA_G
Cuts at: 1943 2088 1943
Size: 145 5193
XmaI C'CCGG_G
Cuts at: 1870 2663 1870
Size: 793 4545
XmnI GAAnn'nnTTC
Cuts at: 3467 3467
Size: 5338
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3146 276
Size: 53 83 186 936 1612 2468
Fragments arranged by size:
2468 1612 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI
PvuII RsrII SacI SacII SapI SexAI SfiI SfoI
SrfI SwaI