Restriction map for
pFN21AE5228
(Circular) (Six-base) MAPSORT of: pFN21AE5228.seq Check: 3455 from: 1 to: 5413
pFN21AE5228 5413 bp
With 182 enzymes: SgfI *
April 26, 2013 18:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2300 2300
Size: 5413
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3223 278
Size: 53 83 186 936 1687 2468
Fragments arranged by size:
2468 1687 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2756 1937
Size: 819 4594
Acc65I G'GTAC_C
Cuts at: 2734 2734
Size: 5413
AclI AA'CG_TT
Cuts at: 1563 3540 3913 1563
Size: 1977 373 3063
Fragments arranged by size:
3063 1977 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3476 4860 5128 1632
Size: 1844 1384 268 1917
Fragments arranged by size:
1917 1844 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5216
AflIII A'CryG_T
Cuts at: 1203 4312 5289 1203
Size: 3109 977 1327
Fragments arranged by size:
3109 1327 977
AgeI A'CCGG_T
Cuts at: 4244 4244
Size: 5413
AhdI GACnn_n'nnGTC
Cuts at: 4142 4142
Size: 5413
AleI CACnn'nnGTG
Cuts at: 1166 2507 1166
Size: 1341 4072
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5413
AlwNI CAG_nnn'CTG
Cuts at: 2272 3156 4728 2272
Size: 884 1572 2957
Fragments arranged by size:
2957 1572 884
ApaLI G'TGCA_C
Cuts at: 3470 4626 3470
Size: 1156 4257
ApoI r'AATT_y
Cuts at: 1493 2722 2948 3100 5209 1493
Size: 1229 226 152 2109 1697
Fragments arranged by size:
2109 1697 1229 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3967 160
Size: 872 2935 1606
Fragments arranged by size:
2935 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5413
AvaI C'yCGr_G
Cuts at: 1870 1943 2533 2738 1870
Size: 73 590 205 4545
Fragments arranged by size:
4545 590 205 73
BamHI G'GATC_C
Cuts at: 2743 3110 2743
Size: 367 5046
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2734 4189 618
Size: 325 217 627 947 1455 1842
Fragments arranged by size:
1842 1455 947 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2732 1391
Size: 1341 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2044 2056 2453 928
Size: 971 145 12 397 3888
Fragments arranged by size:
3888 971 397 145 12
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3604 3638 1903
Size: 34 1667 34 3678
Fragments arranged by size:
3678 1667 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3305 4515 3305
Size: 1210 4203
BclI T'GATC_A
Cuts at: 1548 2646 1548
Size: 1098 4315
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4024 136
Size: 107 122 71 631 605 2352 1525
Fragments arranged by size:
2352 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5408 5408
Size: 5413
BlpI GC'TnA_GC
Cuts at: 2830 2830
Size: 5413
Bme1580I G_kGCm'C
Cuts at: 1790 3474 4630 1790
Size: 1684 1156 2573
Fragments arranged by size:
2573 1684 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5413
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2260 3144 4102
Size: 416 53 83 399 24 843 884 958
Cuts at: 4102 442
Size: 1753
Fragments arranged by size:
1753 958 884 843 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5413
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2097 2165 2464 4073 1239
Size: 858 68 299 1609 2579
Fragments arranged by size:
2579 1609 858 299 68
Bpu10I CC'TnA_GC
Cuts at: 2669 2669
Size: 5413
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2498 3538 4403 4701 2498
Size: 1040 865 298 3210
Fragments arranged by size:
3210 1040 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4076 882
Size: 617 2577 2219
Fragments arranged by size:
2577 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3204 493
Size: 711 2000 2702
Fragments arranged by size:
2702 2000 711
BsaBI GATnn'nnATC
Cuts at: 2651 5407 2651
Size: 2756 2657
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2421 3220 3602 4237
Size: 53 83 186 936 888 799 382 635
Cuts at: 4237 275
Size: 1451
Fragments arranged by size:
1451 936 888 799 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2694 3846 4244 4518 4665 5076 2694
Size: 1152 398 274 147 411 3031
Fragments arranged by size:
3031 1152 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2582 2612 1178
Size: 30 165 30 1179 30 3979
Fragments arranged by size:
3979 1179 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5413
BseYI C'CCAG_C
Cuts at: 2149 2275 4616 5112 2149
Size: 126 2341 496 2450
Fragments arranged by size:
2450 2341 496 126
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2005 1163
Size: 573 269 4571
Fragments arranged by size:
4571 573 269
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2863 3624 4652 1540
Size: 275 186 862 761 1028 2301
Fragments arranged by size:
2301 1028 862 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2514 3474 3559 4630 2514
Size: 960 85 1071 3297
Fragments arranged by size:
3297 1071 960 85
BsmI GAATG_Cn'
Cuts at: 2924 3017 2924
Size: 93 5320
BsmBI CGTCTCn'nnnn_
Cuts at: 2453 2453
Size: 5413
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2514 2732 3474 3559 4630 1391
Size: 399 724 218 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 724 399 218 85
BspEI T'CCGG_A
Cuts at: 2694 5076 2694
Size: 2382 3031
BspHI T'CATG_A
Cuts at: 2217 2649 3302 5032 2217
Size: 432 653 1730 2598
Fragments arranged by size:
2598 1730 653 432
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2049 2300 2768 844
Size: 1205 251 468 3489
Fragments arranged by size:
3489 1205 468 251
BsrBI CCG'CTC
Cuts at: 1394 1823 3300 1394
Size: 429 1477 3507
Fragments arranged by size:
3507 1477 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2622 3908 4082 65
Size: 1183 1374 1286 174 1396
Fragments arranged by size:
1396 1374 1286 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2327 4057 4244 1700
Size: 110 517 1730 187 2869
Fragments arranged by size:
2869 1730 517 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5413
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5413
BssSI C'ACGA_G
Cuts at: 2456 3473 4485 2456
Size: 1017 1012 3384
Fragments arranged by size:
3384 1017 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2028 794
Size: 1234 4179
BstBI TT'CG_AA
Cuts at: 4217 4217
Size: 5413
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5413
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2440 2011
Size: 429 4984
BstYI r'GATC_y
Cuts at: 1972 2743 3110 3495 3512 4964 5050 5062 5408
Size: 771 367 385 17 1452 86 12 346
Cuts at: 5408 1972
Size: 1977
Fragments arranged by size:
1977 1452 771 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2406 513
Size: 552 939 402 3520
Fragments arranged by size:
3520 939 552 402
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2185 487
Size: 683 56 959 3715
Fragments arranged by size:
3715 959 683 56
BtsI GCAGTG_nn'
Cuts at: 2929 3723 3743 2929
Size: 794 20 4599
Fragments arranged by size:
4599 794 20
ClaI AT'CG_AT
Cuts at: 1554 2586 1554
Size: 1032 4381
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5378
DraI TTT'AAA
Cuts at: 2717 2717
Size: 5413
DraIII CAC_nnn'GTG
Cuts at: 2028 2028
Size: 5413
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4420 809
Size: 507 3104 1802
Fragments arranged by size:
3104 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2322 2860 3749
Size: 54 1425 50 275 18 492 538 889
Cuts at: 3749 5378 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 538 492 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2860 1537
Size: 275 1048 4090
Fragments arranged by size:
4090 1048 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2051 2564 2600 3343 1353
Size: 698 513 36 743 3423
Fragments arranged by size:
3423 743 698 513 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2099 4000 4374 4520 387
Size: 1064 138 510 1901 374 146 1280
Fragments arranged by size:
1901 1280 1064 510 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2097 2165 2464 3476 4073 4860 5128
Size: 393 465 68 299 1012 597 787 268
Cuts at: 5128 1239
Size: 1524
Fragments arranged by size:
1524 1012 787 597 465 393 299 268
68
EcoNI CCTnn'n_nnAGG
Cuts at: 2399 2561 2399
Size: 162 5251
EcoO109I rG'GnC_Cy
Cuts at: 2392 2392
Size: 5413
EcoRI G'AATT_C
Cuts at: 2722 3100 2722
Size: 378 5035
FspI TGC'GCA
Cuts at: 3919 3919
Size: 5413
HaeII r_GCGC'y
Cuts at: 1678 2214 4560 1678
Size: 536 2346 2531
Fragments arranged by size:
2531 2346 536
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2070 2102 2582 2614 4054
Size: 32 135 32 498 32 480 32 1440
Cuts at: 4054 4086 4128 4160 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1440 498 480 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2347 2757 3003 669
Size: 988 281 409 410 246 3079
Fragments arranged by size:
3079 988 410 409 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5413
HpaI GTT'AAC
Cuts at: 3003 3003
Size: 5413
KpnI G_GTAC'C
Cuts at: 2738 2738
Size: 5413
MfeI C'AATT_G
Cuts at: 3012 3012
Size: 5413
MluI A'CGCG_T
Cuts at: 5289 5289
Size: 5413
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4527 4711 1305
Size: 3222 184 2007
Fragments arranged by size:
3222 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3591
Fragments arranged by size:
3591 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2204 2507 3371 3730 3889 518
Size: 648 843 195 303 864 359 159 2042
Fragments arranged by size:
2042 864 843 648 359 303 195 159
MspA1I CmG'CkG
Cuts at: 1606 2829 3506 4654 4899 1606
Size: 1223 677 1148 245 2120
Fragments arranged by size:
2120 1223 1148 677 245
NaeI GCC'GGC
Cuts at: 2329 2329
Size: 5413
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3922
Fragments arranged by size:
3922 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5413
NgoMIV G'CCGG_C
Cuts at: 2327 2327
Size: 5413
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5413
NotI GC'GGCC_GC
Cuts at: 2860 2860
Size: 5413
NspI r_CATG'y
Cuts at: 1132 1229 2771 4316 1132
Size: 97 1542 1545 2229
Fragments arranged by size:
2229 1545 1542 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5413
PciI A'CATG_T
Cuts at: 4312 4312
Size: 5413
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5413
PfoI T'CCnGG_A
Cuts at: 2256 2600 2256
Size: 344 5069
PmeI GTTT'AAAC
Cuts at: 2717 2717
Size: 5413
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3482 3514 4995 5027 3482
Size: 32 1481 32 3868
Fragments arranged by size:
3868 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5413
PsiI TTA'TAA
Cuts at: 2983 2983
Size: 5413
PstI C_TGCA'G
Cuts at: 830 2765 830
Size: 1935 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5413
SalI G'TCGA_C
Cuts at: 1936 2755 1936
Size: 819 4594
SapI GCTCTTCn'nnn_
Cuts at: 2051 2051
Size: 5413
SbfI CC_TGCA'GG
Cuts at: 2765 2765
Size: 5413
ScaI AGT'ACT
Cuts at: 3661 5373 3661
Size: 1712 3701
SfcI C'TryA_G
Cuts at: 826 1045 2396 2761 3896 4577 4768 826
Size: 219 1351 365 1135 681 191 1471
Fragments arranged by size:
1471 1351 1135 681 365 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5413
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5413
SmaI CCC'GGG
Cuts at: 1872 2740 1872
Size: 868 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2513 3517 4418 4680 820
Size: 197 926 570 1004 901 262 1553
Fragments arranged by size:
1553 1004 926 901 570 262 197
SnaBI TAC'GTA
Cuts at: 493 3204 493
Size: 2711 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5413
SphI G_CATG'C
Cuts at: 1132 2771 1132
Size: 1639 3774
SspI AAT'ATT
Cuts at: 5 52 3337 5
Size: 47 3285 2081
Fragments arranged by size:
3285 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5413
StyI C'CwwG_G
Cuts at: 513 1065 2004 2364 2433 2852 513
Size: 552 939 360 69 419 3074
Fragments arranged by size:
3074 939 552 419 360 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2081 3440 3457 3610 3795 4240 325
Size: 1255 501 1359 17 153 185 445 1498
Fragments arranged by size:
1498 1359 1255 501 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3659 5371 96
Size: 274 80 33 51 1443 1682 1712 138
Fragments arranged by size:
1712 1682 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2252 2284 5373 5405 2252
Size: 32 3089 32 2260
Fragments arranged by size:
3089 2260 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5413
XbaI T'CTAG_A
Cuts at: 2749 2749
Size: 5413
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5413
XmaI C'CCGG_G
Cuts at: 1870 2738 1870
Size: 868 4545
XmnI GAAnn'nnTTC
Cuts at: 2378 2450 3542 2378
Size: 72 1092 4249
Fragments arranged by size:
4249 1092 72
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3221 276
Size: 53 83 186 936 1687 2468
Fragments arranged by size:
2468 1687 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI