Restriction map for
pFN21AE5225
(Circular) (Six-base) MAPSORT of: pFN21AE5225.seq Check: 8585 from: 1 to: 5455
pFN21AE5225 5455 bp
With 182 enzymes: SgfI *
January 30, 2014 17:15 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3265 278
Size: 53 83 186 936 1729 2468
Fragments arranged by size:
2468 1729 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2798 1937
Size: 861 4594
Acc65I G'GTAC_C
Cuts at: 2776 2776
Size: 5455
AclI AA'CG_TT
Cuts at: 1563 3582 3955 1563
Size: 2019 373 3063
Fragments arranged by size:
3063 2019 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3518 4902 5170 1632
Size: 1886 1384 268 1917
Fragments arranged by size:
1917 1886 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5258
AflIII A'CryG_T
Cuts at: 1203 2412 2634 4354 5331 1203
Size: 1209 222 1720 977 1327
Fragments arranged by size:
1720 1327 1209 977 222
AgeI A'CCGG_T
Cuts at: 4286 4286
Size: 5455
AhdI GACnn_n'nnGTC
Cuts at: 4184 4184
Size: 5455
AleI CACnn'nnGTG
Cuts at: 1166 2635 1166
Size: 1469 3986
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5455
AlwNI CAG_nnn'CTG
Cuts at: 3198 4770 3198
Size: 1572 3883
ApaI G_GGCC'C
Cuts at: 2018 2100 2018
Size: 82 5373
ApaLI G'TGCA_C
Cuts at: 3512 4668 3512
Size: 1156 4299
ApoI r'AATT_y
Cuts at: 1493 2764 2990 3142 5251 1493
Size: 1271 226 152 2109 1697
Fragments arranged by size:
2109 1697 1271 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4009 160
Size: 872 2977 1606
Fragments arranged by size:
2977 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5455
AvaI C'yCGr_G
Cuts at: 1870 1943 2072 2093 2129 2724 2780 1870
Size: 73 129 21 36 595 56 4545
Fragments arranged by size:
4545 595 129 73 56 36 21
BamHI G'GATC_C
Cuts at: 2080 2713 2785 3152 2080
Size: 633 72 367 4383
Fragments arranged by size:
4383 633 367 72
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2026 2048 2776 4231 618
Size: 325 217 627 239 22 728 1455 1842
Fragments arranged by size:
1842 1455 728 627 325 239 217 22
BanII G_rGCy'C
Cuts at: 1391 2018 2100 2246 2774 1391
Size: 627 82 146 528 4072
Fragments arranged by size:
4072 627 528 146 82
BbeI G_GCGC'C
Cuts at: 2052 2052
Size: 5455
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2542 928
Size: 971 643 3841
Fragments arranged by size:
3841 971 643
BbvCI CC'TCA_GC
Cuts at: 2498 2498
Size: 5455
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3646 3680 1903
Size: 34 1709 34 3678
Fragments arranged by size:
3678 1709 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3347 4557 3347
Size: 1210 4245
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5455
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2104 2125 4066
Size: 107 122 71 631 605 432 21 1941
Cuts at: 4066 136
Size: 1525
Fragments arranged by size:
1941 1525 631 605 432 122 107 71
21
BglII A'GATC_T
Cuts at: 5450 5450
Size: 5455
BlpI GC'TnA_GC
Cuts at: 2872 2872
Size: 5455
Bme1580I G_kGCm'C
Cuts at: 1790 2018 2029 2100 3516 4672 1790
Size: 228 11 71 1416 1156 2573
Fragments arranged by size:
2573 1416 1156 228 71 11
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5455
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3186 4144 442
Size: 416 53 83 399 24 1769 958 1753
Fragments arranged by size:
1769 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5455
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2359 2359
Size: 5455
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2311 2625 4115 1239
Size: 1072 314 1490 2579
Fragments arranged by size:
2579 1490 1072 314
Bpu10I CC'TnA_GC
Cuts at: 2240 2498 2240
Size: 258 5197
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2264 3580 4445 4743 2264
Size: 1316 865 298 2976
Fragments arranged by size:
2976 1316 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2330 4118 882
Size: 617 831 1788 2219
Fragments arranged by size:
2219 1788 831 617
BsaAI yAC'GTr
Cuts at: 493 1204 3246 493
Size: 711 2042 2702
Fragments arranged by size:
2702 2042 711
BsaBI GATnn'nnATC
Cuts at: 2376 5449 2376
Size: 3073 2382
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2049 2285 3262 3644
Size: 53 83 186 936 516 236 977 382
Cuts at: 3644 4279 275
Size: 635 1451
Fragments arranged by size:
1451 977 936 635 516 382 236 186
83 53
BsaWI w'CCGG_w
Cuts at: 3888 4286 4560 4707 5118 3888
Size: 398 274 147 411 4225
Fragments arranged by size:
4225 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2598 2628 1178
Size: 30 165 30 1195 30 4005
Fragments arranged by size:
4005 1195 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2201 1186
Size: 1015 4440
BseYI C'CCAG_C
Cuts at: 2099 2248 2262 4658 5154 2099
Size: 149 14 2396 496 2400
Fragments arranged by size:
2400 2396 496 149 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2077 1163
Size: 573 341 4541
Fragments arranged by size:
4541 573 341
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2905 3666 4694 1540
Size: 275 186 904 761 1028 2301
Fragments arranged by size:
2301 1028 904 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2246 3516 3601 4672 2246
Size: 1270 85 1071 3029
Fragments arranged by size:
3029 1270 1071 85
BsmI GAATG_Cn'
Cuts at: 2966 3059 2966
Size: 93 5362
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2029 2100 2246 2774 3516 3601
Size: 399 228 11 71 146 528 742 85
Cuts at: 3601 4672 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 528 399 228 146 85
71 11
BspEI T'CCGG_A
Cuts at: 5118 5118
Size: 5455
BspHI T'CATG_A
Cuts at: 2380 3344 5074 2380
Size: 964 1730 2761
Fragments arranged by size:
2761 1730 964
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2810 844
Size: 1966 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2077 2173 3342 1394
Size: 429 254 96 1169 3507
Fragments arranged by size:
3507 1169 429 254 96
BsrDI GCAATG_nn'
Cuts at: 65 1248 2068 3950 4124 65
Size: 1183 820 1882 174 1396
Fragments arranged by size:
1882 1396 1183 820 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2029 2107 4099 4286 1700
Size: 110 219 78 1992 187 2869
Fragments arranged by size:
2869 1992 219 187 110 78
BsrGI T'GTAC_A
Cuts at: 96 2656 96
Size: 2560 2895
BssHII G'CGCG_C
Cuts at: 1925 2142 1925
Size: 217 5238
BssSI C'ACGA_G
Cuts at: 3515 4527 3515
Size: 1012 4443
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2275 794
Size: 1481 3974
BstBI TT'CG_AA
Cuts at: 4259 4259
Size: 5455
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5455
BstYI r'GATC_y
Cuts at: 1972 2080 2713 2785 3152 3537 3554 5006 5092
Size: 108 633 72 367 385 17 1452 86
Cuts at: 5092 5104 5450 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 633 385 367 346 108 86
72 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2042 2087 2123 2151 513
Size: 552 939 38 45 36 28 3817
Fragments arranged by size:
3817 939 552 45 38 36 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4716
Fragments arranged by size:
4716 683 56
BtsI GCAGTG_nn'
Cuts at: 2971 3765 3785 2971
Size: 794 20 4641
Fragments arranged by size:
4641 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5455
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5420
DraI TTT'AAA
Cuts at: 2759 2759
Size: 5455
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4462 809
Size: 507 3146 1802
Fragments arranged by size:
3146 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2304 2902 3791
Size: 54 1425 50 275 18 474 598 889
Cuts at: 3791 5420 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 598 474 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2902 1537
Size: 275 1090 4090
Fragments arranged by size:
4090 1090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2724 3385 1353
Size: 1371 661 3423
Fragments arranged by size:
3423 1371 661
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2048 2073 2126 4042 4416 4562
Size: 1064 138 459 25 53 1916 374 146
Cuts at: 4562 387
Size: 1280
Fragments arranged by size:
1916 1280 1064 459 374 146 138 53
25
EcoICRI GAG'CTC
Cuts at: 2244 2244
Size: 5455
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2311 2625 3518 4115 4902 5170 1239
Size: 393 679 314 893 597 787 268 1524
Fragments arranged by size:
1524 893 787 679 597 393 314 268
EcoO109I rG'GnC_Cy
Cuts at: 2014 2015 2014
Size: 1 5454
EcoRI G'AATT_C
Cuts at: 2764 3142 2764
Size: 378 5077
FspI TGC'GCA
Cuts at: 3961 3961
Size: 5455
HaeII r_GCGC'y
Cuts at: 1678 2052 2557 4602 1678
Size: 374 505 2045 2531
Fragments arranged by size:
2531 2045 505 374
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4096 4128 4170 4202 1373
Size: 32 135 32 2524 32 42 32 2626
Fragments arranged by size:
2626 2524 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2799 3045 669
Size: 988 281 861 246 3079
Fragments arranged by size:
3079 988 861 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5455
HpaI GTT'AAC
Cuts at: 3045 3045
Size: 5455
KasI G'GCGC_C
Cuts at: 2048 2048
Size: 5455
KpnI G_GTAC'C
Cuts at: 2780 2780
Size: 5455
MfeI C'AATT_G
Cuts at: 3054 3054
Size: 5455
MluI A'CGCG_T
Cuts at: 5331 5331
Size: 5455
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2431 4569 4753 1305
Size: 1126 2138 184 2007
Fragments arranged by size:
2138 2007 1126 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3633
Fragments arranged by size:
3633 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2411 2635 2639 3413 3772 3931 518
Size: 648 1245 224 4 774 359 159 2042
Fragments arranged by size:
2042 1245 774 648 359 224 159 4
MspA1I CmG'CkG
Cuts at: 1606 2044 2089 2125 2153 2266 2871 3548 4696
Size: 438 45 36 28 113 605 677 1148
Cuts at: 4696 4941 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 605 438 245 113 45
36 28
NaeI GCC'GGC
Cuts at: 2109 2109
Size: 5455
NarI GG'CG_CC
Cuts at: 2049 2049
Size: 5455
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3964
Fragments arranged by size:
3964 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5455
NgoMIV G'CCGG_C
Cuts at: 2107 2107
Size: 5455
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5455
NotI GC'GGCC_GC
Cuts at: 2902 2902
Size: 5455
NspI r_CATG'y
Cuts at: 1132 1229 2416 2638 2813 4358 1132
Size: 97 1187 222 175 1545 2229
Fragments arranged by size:
2229 1545 1187 222 175 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5455
PciI A'CATG_T
Cuts at: 2412 2634 4354 2412
Size: 222 1720 3513
Fragments arranged by size:
3513 1720 222
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2450 1108
Size: 1342 4113
PfoI T'CCnGG_A
Cuts at: 2706 2706
Size: 5455
PmeI GTTT'AAAC
Cuts at: 2759 2759
Size: 5455
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3524 3556 5037 5069 3524
Size: 32 1481 32 3910
Fragments arranged by size:
3910 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5455
PsiI TTA'TAA
Cuts at: 3025 3025
Size: 5455
PspOMI G'GGCC_C
Cuts at: 2014 2096 2014
Size: 82 5373
PstI C_TGCA'G
Cuts at: 830 2807 830
Size: 1977 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5455
SacI G_AGCT'C
Cuts at: 2246 2246
Size: 5455
SacII CC_GC'GG
Cuts at: 2045 2090 2126 2154 2045
Size: 45 36 28 5346
Fragments arranged by size:
5346 45 36 28
SalI G'TCGA_C
Cuts at: 1936 2797 1936
Size: 861 4594
SbfI CC_TGCA'GG
Cuts at: 2807 2807
Size: 5455
ScaI AGT'ACT
Cuts at: 3703 5415 3703
Size: 1712 3743
SfcI C'TryA_G
Cuts at: 826 1045 2527 2574 2803 3938 4619 4810 826
Size: 219 1482 47 229 1135 681 191 1471
Fragments arranged by size:
1482 1471 1135 681 229 219 191 47
SfiI GGCCn_nnn'nGGCC
Cuts at: 2104 2104
Size: 5455
SfoI GGC'GCC
Cuts at: 2050 2050
Size: 5455
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5455
SgrAI Cr'CCGG_yG
Cuts at: 1700 2029 1700
Size: 329 5126
SmaI CCC'GGG
Cuts at: 1872 2095 2782 1872
Size: 223 687 4545
Fragments arranged by size:
4545 687 223
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2279 3559 4460 4722 820
Size: 197 926 336 1280 901 262 1553
Fragments arranged by size:
1553 1280 926 901 336 262 197
SnaBI TAC'GTA
Cuts at: 493 3246 493
Size: 2753 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5455
SphI G_CATG'C
Cuts at: 1132 2813 1132
Size: 1681 3774
SrfI GCCC'GGGC
Cuts at: 2095 2095
Size: 5455
SspI AAT'ATT
Cuts at: 5 52 3379 5
Size: 47 3327 2081
Fragments arranged by size:
3327 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5455
StyI C'CwwG_G
Cuts at: 513 1065 2004 2322 2491 2894 513
Size: 552 939 318 169 403 3074
Fragments arranged by size:
3074 939 552 403 318 169
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2687 3482 3499 3652 3837 4282 325
Size: 1255 1107 795 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1107 795 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2656 3701 5413
Size: 274 80 33 51 1443 679 1045 1712
Cuts at: 5413 96
Size: 138
Fragments arranged by size:
1712 1443 1045 679 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5415 5447 5415
Size: 32 5423
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5455
XbaI T'CTAG_A
Cuts at: 2791 2791
Size: 5455
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2692 2692
Size: 5455
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5455
XmaI C'CCGG_G
Cuts at: 1870 2093 2780 1870
Size: 223 687 4545
Fragments arranged by size:
4545 687 223
XmnI GAAnn'nnTTC
Cuts at: 3584 3584
Size: 5455
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3263 276
Size: 53 83 186 936 1729 2468
Fragments arranged by size:
2468 1729 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SacI SacII SalI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI
PsrI PvuII RsrII SanDI SapI SexAI SwaI