Restriction map for
pFN21AE5216
(Circular) (Six-base) MAPSORT of: pFN21AE5216.seq Check: 4930 from: 1 to: 5302
pFN21AE5216 5302 bp
With 182 enzymes: SgfI *
January 30, 2014 17:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2428 2428
Size: 5302
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3112 278
Size: 53 83 186 936 1576 2468
Fragments arranged by size:
2468 1576 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2028 2645 1937
Size: 91 617 4594
Fragments arranged by size:
4594 617 91
Acc65I G'GTAC_C
Cuts at: 2623 2623
Size: 5302
AclI AA'CG_TT
Cuts at: 1563 3429 3802 1563
Size: 1866 373 3063
Fragments arranged by size:
3063 1866 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2043 3365 4749 5017 1632
Size: 411 1322 1384 268 1917
Fragments arranged by size:
1917 1384 1322 411 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5105
AflIII A'CryG_T
Cuts at: 1203 2159 4201 5178 1203
Size: 956 2042 977 1327
Fragments arranged by size:
2042 1327 977 956
AgeI A'CCGG_T
Cuts at: 4133 4133
Size: 5302
AhdI GACnn_n'nnGTC
Cuts at: 4031 4031
Size: 5302
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5302
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5302
AlwNI CAG_nnn'CTG
Cuts at: 2485 3045 4617 2485
Size: 560 1572 3170
Fragments arranged by size:
3170 1572 560
ApaLI G'TGCA_C
Cuts at: 3359 4515 3359
Size: 1156 4146
ApoI r'AATT_y
Cuts at: 1493 2611 2837 2989 5098 1493
Size: 1118 226 152 2109 1697
Fragments arranged by size:
2109 1697 1118 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3856 160
Size: 872 2824 1606
Fragments arranged by size:
2824 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5302
AvaI C'yCGr_G
Cuts at: 1870 1943 2292 2627 1870
Size: 73 349 335 4545
Fragments arranged by size:
4545 349 335 73
BamHI G'GATC_C
Cuts at: 2632 2999 2632
Size: 367 4935
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2623 4078 618
Size: 325 217 627 836 1455 1842
Fragments arranged by size:
1842 1455 836 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2049 2325 2336 2513 2621 1391
Size: 658 276 11 177 108 4072
Fragments arranged by size:
4072 658 276 177 108 11
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4331
BbvCI CC'TCA_GC
Cuts at: 2311 2311
Size: 5302
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2056 2090 2144 2178 3493 3527 1903
Size: 34 119 34 54 34 1315 34 3678
Fragments arranged by size:
3678 1315 119 54 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3194 4404 3194
Size: 1210 4092
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5302
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3913 136
Size: 107 122 71 631 605 2241 1525
Fragments arranged by size:
2241 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5297 5297
Size: 5302
BlpI GC'TnA_GC
Cuts at: 2719 2719
Size: 5302
Bme1580I G_kGCm'C
Cuts at: 1790 3363 4519 1790
Size: 1573 1156 2573
Fragments arranged by size:
2573 1573 1156
BmgBI CAC'GTC
Cuts at: 1318 2160 2242 1318
Size: 842 82 4378
Fragments arranged by size:
4378 842 82
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2351 2516 3033
Size: 416 53 83 399 24 934 165 517
Cuts at: 3033 3991 442
Size: 958 1753
Fragments arranged by size:
1753 958 934 517 416 399 165 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5302
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2546 3962 1239
Size: 1307 1416 2579
Fragments arranged by size:
2579 1416 1307
Bpu10I CC'TnA_GC
Cuts at: 2311 2311
Size: 5302
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2384 3427 4292 4590 2384
Size: 1043 865 298 3096
Fragments arranged by size:
3096 1043 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3965 882
Size: 617 2466 2219
Fragments arranged by size:
2466 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3093 493
Size: 711 1889 2702
Fragments arranged by size:
2702 1889 711
BsaBI GATnn'nnATC
Cuts at: 5296 5296
Size: 5302
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3109 3491 4126 275
Size: 53 83 186 936 1576 382 635 1451
Fragments arranged by size:
1576 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2368 3735 4133 4407 4554 4965 2368
Size: 1367 398 274 147 411 2705
Fragments arranged by size:
2705 1367 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2010 2021 2040 2051 1178
Size: 30 165 30 607 11 19 11 4429
Fragments arranged by size:
4429 607 165 30 30 19 11 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2174 1186
Size: 988 4314
BseYI C'CCAG_C
Cuts at: 2149 2349 4505 5001 2149
Size: 200 2156 496 2450
Fragments arranged by size:
2450 2156 496 200
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2443 1163
Size: 573 707 4022
Fragments arranged by size:
4022 707 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2752 3513 4541 1540
Size: 275 186 751 761 1028 2301
Fragments arranged by size:
2301 1028 761 751 275 186
BsiHKAI G_wGCw'C
Cuts at: 2301 2325 2376 3363 3448 4519 2301
Size: 24 51 987 85 1071 3084
Fragments arranged by size:
3084 1071 987 85 51 24
BsmI GAATG_Cn'
Cuts at: 2272 2813 2906 2272
Size: 541 93 4668
Fragments arranged by size:
4668 541 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2057 2150 2057
Size: 93 5209
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2301 2325 2336 2376 2513 2621
Size: 399 259 252 24 11 40 137 108
Cuts at: 2621 3363 3448 4519 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 259 252 137 108
85 40 24 11
BspEI T'CCGG_A
Cuts at: 2368 4965 2368
Size: 2597 2705
BspHI T'CATG_A
Cuts at: 3191 4921 3191
Size: 1730 3572
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2350 2428 2657 844
Size: 1506 78 229 3489
Fragments arranged by size:
3489 1506 229 78
BsrBI CCG'CTC
Cuts at: 1394 1823 2092 2261 3189 1394
Size: 429 269 169 928 3507
Fragments arranged by size:
3507 928 429 269 169
BsrDI GCAATG_nn'
Cuts at: 65 1248 3797 3971 65
Size: 1183 2549 174 1396
Fragments arranged by size:
2549 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3946 4133 1700
Size: 110 2136 187 2869
Fragments arranged by size:
2869 2136 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5302
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5302
BssSI C'ACGA_G
Cuts at: 3362 4374 3362
Size: 1012 4290
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5302
BstBI TT'CG_AA
Cuts at: 4106 4106
Size: 5302
BstEII G'GTnAC_C
Cuts at: 1184 2138 1184
Size: 954 4348
BstXI CCAn_nnnn'nTGG
Cuts at: 2546 2546
Size: 5302
BstYI r'GATC_y
Cuts at: 1972 2632 2999 3384 3401 4853 4939 4951 5297
Size: 660 367 385 17 1452 86 12 346
Cuts at: 5297 1972
Size: 1977
Fragments arranged by size:
1977 1452 660 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3811
Fragments arranged by size:
3811 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4563
Fragments arranged by size:
4563 683 56
BtsI GCAGTG_nn'
Cuts at: 2818 3612 3632 2818
Size: 794 20 4488
Fragments arranged by size:
4488 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5302
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5267
DraI TTT'AAA
Cuts at: 2606 2606
Size: 5302
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4309 809
Size: 507 2993 1802
Fragments arranged by size:
2993 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2749 3638 5267
Size: 54 1425 50 275 18 919 889 1629
Cuts at: 5267 8
Size: 43
Fragments arranged by size:
1629 1425 919 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2749 1537
Size: 275 937 4090
Fragments arranged by size:
4090 937 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3232 1353
Size: 1879 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2075 3889 4263 4409 387
Size: 1064 138 486 1814 374 146 1280
Fragments arranged by size:
1814 1280 1064 486 374 146 138
EcoICRI GAG'CTC
Cuts at: 2323 2323
Size: 5302
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2043 2546 3365 3962 4749 5017 1239
Size: 393 411 503 819 597 787 268 1524
Fragments arranged by size:
1524 819 787 597 503 411 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2572 2572
Size: 5302
EcoRI G'AATT_C
Cuts at: 2611 2989 2611
Size: 378 4924
FspI TGC'GCA
Cuts at: 2579 3808 2579
Size: 1229 4073
HaeII r_GCGC'y
Cuts at: 1678 4449 1678
Size: 2771 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2010 2021 2042 2053 2090
Size: 32 135 32 438 11 21 11 37
Cuts at: 2090 2122 3943 3975 4017 4049 1373
Size: 32 1821 32 42 32 2626
Fragments arranged by size:
2626 1821 438 135 42 37 32 32
32 32 32 21 11 11
HincII GTy'rAC
Cuts at: 669 1657 1938 2029 2646 2892 669
Size: 988 281 91 617 246 3079
Fragments arranged by size:
3079 988 617 281 246 91
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5302
HpaI GTT'AAC
Cuts at: 2892 2892
Size: 5302
KpnI G_GTAC'C
Cuts at: 2627 2627
Size: 5302
MfeI C'AATT_G
Cuts at: 2901 2901
Size: 5302
MluI A'CGCG_T
Cuts at: 5178 5178
Size: 5302
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4416 4600 1305
Size: 3111 184 2007
Fragments arranged by size:
3111 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3480
Fragments arranged by size:
3480 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3260 3619 3778 518
Size: 648 2094 359 159 2042
Fragments arranged by size:
2094 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2200 2305 2542 2718 3395 4543 4788 1606
Size: 594 105 237 176 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 594 245 237 176 105
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3811
Fragments arranged by size:
3811 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5302
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5302
NotI GC'GGCC_GC
Cuts at: 2749 2749
Size: 5302
NspI r_CATG'y
Cuts at: 1132 1229 2149 2565 2660 4205 1132
Size: 97 920 416 95 1545 2229
Fragments arranged by size:
2229 1545 920 416 97 95
PasI CC'CwG_GG
Cuts at: 1348 2329 2410 1348
Size: 981 81 4240
Fragments arranged by size:
4240 981 81
PciI A'CATG_T
Cuts at: 4201 4201
Size: 5302
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5302
PmeI GTTT'AAAC
Cuts at: 2606 2606
Size: 5302
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3371 3403 4884 4916 3371
Size: 32 1481 32 3757
Fragments arranged by size:
3757 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5302
PsiI TTA'TAA
Cuts at: 2872 2872
Size: 5302
PstI C_TGCA'G
Cuts at: 830 2079 2392 2589 2654 830
Size: 1249 313 197 65 3478
Fragments arranged by size:
3478 1249 313 197 65
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5302
PvuII CAG'CTG
Cuts at: 2305 2542 2305
Size: 237 5065
SacI G_AGCT'C
Cuts at: 2325 2325
Size: 5302
SalI G'TCGA_C
Cuts at: 1936 2027 2644 1936
Size: 91 617 4594
Fragments arranged by size:
4594 617 91
SbfI CC_TGCA'GG
Cuts at: 2654 2654
Size: 5302
ScaI AGT'ACT
Cuts at: 3550 5262 3550
Size: 1712 3590
SfcI C'TryA_G
Cuts at: 826 1045 2075 2388 2585 2594 2650 3785 4466
Size: 219 1030 313 197 9 56 1135 681
Cuts at: 4466 4657 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1030 681 313 219 197 191
56 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5302
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5302
SmaI CCC'GGG
Cuts at: 1872 2629 1872
Size: 757 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2399 3406 4307 4569 820
Size: 197 926 456 1007 901 262 1553
Fragments arranged by size:
1553 1007 926 901 456 262 197
SnaBI TAC'GTA
Cuts at: 493 3093 493
Size: 2600 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5302
SphI G_CATG'C
Cuts at: 1132 2149 2660 1132
Size: 1017 511 3774
Fragments arranged by size:
3774 1017 511
SspI AAT'ATT
Cuts at: 5 52 3226 5
Size: 47 3174 2081
Fragments arranged by size:
3174 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5302
StyI C'CwwG_G
Cuts at: 513 1065 2004 2741 513
Size: 552 939 737 3074
Fragments arranged by size:
3074 939 737 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3329 3346 3499 3684 4129 325
Size: 1255 1749 17 153 185 445 1498
Fragments arranged by size:
1749 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3548 5260 96
Size: 274 80 33 51 1443 1571 1712 138
Fragments arranged by size:
1712 1571 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5262 5294 5262
Size: 32 5270
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5302
XbaI T'CTAG_A
Cuts at: 2638 2638
Size: 5302
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5302
XmaI C'CCGG_G
Cuts at: 1870 2627 1870
Size: 757 4545
XmnI GAAnn'nnTTC
Cuts at: 3431 3431
Size: 5302
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3110 276
Size: 53 83 186 936 1576 2468
Fragments arranged by size:
2468 1576 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI