Restriction map for
pFN21AE5214
(Circular) (Six-base) MAPSORT of: pFN21AE5214.seq Check: 1227 from: 1 to: 5350
pFN21AE5214 5350 bp
With 182 enzymes: SgfI *
January 30, 2014 17:15 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3160 278
Size: 53 83 186 936 1624 2468
Fragments arranged by size:
2468 1624 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2693 1937
Size: 756 4594
Acc65I G'GTAC_C
Cuts at: 2671 2671
Size: 5350
AclI AA'CG_TT
Cuts at: 1563 3477 3850 1563
Size: 1914 373 3063
Fragments arranged by size:
3063 1914 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2023 2294 2612 3413 4797 5065 1632
Size: 391 271 318 801 1384 268 1917
Fragments arranged by size:
1917 1384 801 391 318 271 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5153
AflIII A'CryG_T
Cuts at: 1203 4249 5226 1203
Size: 3046 977 1327
Fragments arranged by size:
3046 1327 977
AgeI A'CCGG_T
Cuts at: 4181 4181
Size: 5350
AhdI GACnn_n'nnGTC
Cuts at: 4079 4079
Size: 5350
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5350
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2031 1184
Size: 847 4503
AlwNI CAG_nnn'CTG
Cuts at: 2108 2575 3093 4665 2108
Size: 467 518 1572 2793
Fragments arranged by size:
2793 1572 518 467
ApaLI G'TGCA_C
Cuts at: 3407 4563 3407
Size: 1156 4194
ApoI r'AATT_y
Cuts at: 1493 2659 2885 3037 5146 1493
Size: 1166 226 152 2109 1697
Fragments arranged by size:
2109 1697 1166 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3904 160
Size: 872 2872 1606
Fragments arranged by size:
2872 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5350
AvaI C'yCGr_G
Cuts at: 1870 1943 2675 1870
Size: 73 732 4545
Fragments arranged by size:
4545 732 73
BamHI G'GATC_C
Cuts at: 2680 3047 2680
Size: 367 4983
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2074 2492 2642 2671 4126
Size: 325 217 627 287 418 150 29 1455
Cuts at: 4126 618
Size: 1842
Fragments arranged by size:
1842 1455 627 418 325 287 217 150
29
BanII G_rGCy'C
Cuts at: 1391 2055 2457 2669 1391
Size: 664 402 212 4072
Fragments arranged by size:
4072 664 402 212
BbeI G_GCGC'C
Cuts at: 2646 2646
Size: 5350
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2600 928
Size: 971 701 3678
Fragments arranged by size:
3678 971 701
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3541 3575 1903
Size: 34 50 34 1520 34 3678
Fragments arranged by size:
3678 1520 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3242 4452 3242
Size: 1210 4140
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5350
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2415 3961 136
Size: 107 122 71 631 605 743 1546 1525
Fragments arranged by size:
1546 1525 743 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5345 5345
Size: 5350
BlpI GC'TnA_GC
Cuts at: 2576 2767 2576
Size: 191 5159
Bme1580I G_kGCm'C
Cuts at: 1790 3411 4567 1790
Size: 1621 1156 2573
Fragments arranged by size:
2573 1621 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5350
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3081 4039 442
Size: 416 53 83 399 24 1664 958 1753
Fragments arranged by size:
1753 1664 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5350
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2485 2485
Size: 5350
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2231 2300 4010 1239
Size: 992 69 1710 2579
Fragments arranged by size:
2579 1710 992 69
Bpu10I CC'TnA_GC
Cuts at: 2460 2460
Size: 5350
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3475 4340 4638 3475
Size: 865 298 4187
Fragments arranged by size:
4187 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4013 882
Size: 617 2514 2219
Fragments arranged by size:
2514 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3141 493
Size: 711 1937 2702
Fragments arranged by size:
2702 1937 711
BsaBI GATnn'nnATC
Cuts at: 5344 5344
Size: 5350
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2139 2643 3157 3539
Size: 53 83 186 936 606 504 514 382
Cuts at: 3539 4174 275
Size: 635 1451
Fragments arranged by size:
1451 936 635 606 514 504 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3783 4181 4455 4602 5013 3783
Size: 398 274 147 411 4120
Fragments arranged by size:
4120 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5125
Fragments arranged by size:
5125 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2249 2569 2615 2618 1186
Size: 1063 320 46 3 3918
Fragments arranged by size:
3918 1063 320 46 3
BseYI C'CCAG_C
Cuts at: 2318 4553 5049 2318
Size: 2235 496 2619
Fragments arranged by size:
2619 2235 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2137 2375 1163
Size: 573 401 238 4138
Fragments arranged by size:
4138 573 401 238
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2800 3561 4589 1540
Size: 275 186 799 761 1028 2301
Fragments arranged by size:
2301 1028 799 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2582 3411 3496 4567 2582
Size: 829 85 1071 3365
Fragments arranged by size:
3365 1071 829 85
BsmI GAATG_Cn'
Cuts at: 2861 2954 2861
Size: 93 5257
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 2457 2582 2669 3411 3496 4567
Size: 399 265 402 125 87 742 85 1071
Cuts at: 4567 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 402 399 265 125 87
85
BspEI T'CCGG_A
Cuts at: 5013 5013
Size: 5350
BspHI T'CATG_A
Cuts at: 2391 3239 4969 2391
Size: 848 1730 2772
Fragments arranged by size:
2772 1730 848
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2116 2428 2705 844
Size: 1272 312 277 3489
Fragments arranged by size:
3489 1272 312 277
BsrBI CCG'CTC
Cuts at: 1394 1823 2284 2464 3237 1394
Size: 429 461 180 773 3507
Fragments arranged by size:
3507 773 461 429 180
BsrDI GCAATG_nn'
Cuts at: 65 1248 3845 4019 65
Size: 1183 2597 174 1396
Fragments arranged by size:
2597 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3994 4181 1700
Size: 110 2184 187 2869
Fragments arranged by size:
2869 2184 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5350
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5350
BssSI C'ACGA_G
Cuts at: 3410 4422 3410
Size: 1012 4338
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2226 794
Size: 1432 3918
BstBI TT'CG_AA
Cuts at: 4154 4154
Size: 5350
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5350
BstYI r'GATC_y
Cuts at: 1972 2427 2680 3047 3432 3449 4901 4987 4999
Size: 455 253 367 385 17 1452 86 12
Cuts at: 4999 5345 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 455 385 367 346 253 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2413 2419 2472 513
Size: 552 1348 6 53 3391
Fragments arranged by size:
3391 1348 552 53 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4611
Fragments arranged by size:
4611 683 56
BtsI GCAGTG_nn'
Cuts at: 2866 3660 3680 2866
Size: 794 20 4536
Fragments arranged by size:
4536 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5350
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5315
DraI TTT'AAA
Cuts at: 2654 2654
Size: 5350
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4357 809
Size: 507 3041 1802
Fragments arranged by size:
3041 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2416 2478 2797
Size: 54 1425 50 275 18 586 62 319
Cuts at: 2797 3686 5315 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 586 319 275 62 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2797 1537
Size: 275 985 4090
Fragments arranged by size:
4090 985 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2445 3280 1353
Size: 1092 835 3423
Fragments arranged by size:
3423 1092 835
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3937 4311 4457 387
Size: 1064 138 2348 374 146 1280
Fragments arranged by size:
2348 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2023 2231 2294 2300 2612 3413 4010
Size: 393 391 208 63 6 312 801 597
Cuts at: 4010 4797 5065 1239
Size: 787 268 1524
Fragments arranged by size:
1524 801 787 597 393 391 312 268
208 63 6
EcoO109I rG'GnC_Cy
Cuts at: 2440 2440
Size: 5350
EcoRI G'AATT_C
Cuts at: 2659 3037 2659
Size: 378 4972
FspI TGC'GCA
Cuts at: 3856 3856
Size: 5350
HaeII r_GCGC'y
Cuts at: 1678 2646 4497 1678
Size: 968 1851 2531
Fragments arranged by size:
2531 1851 968
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3991 4023 4065 4097 1373
Size: 32 135 32 2419 32 42 32 2626
Fragments arranged by size:
2626 2419 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2694 2940 669
Size: 988 281 756 246 3079
Fragments arranged by size:
3079 988 756 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5350
HpaI GTT'AAC
Cuts at: 2940 2940
Size: 5350
KasI G'GCGC_C
Cuts at: 2642 2642
Size: 5350
KpnI G_GTAC'C
Cuts at: 2675 2675
Size: 5350
MfeI C'AATT_G
Cuts at: 2949 2949
Size: 5350
MluI A'CGCG_T
Cuts at: 5226 5226
Size: 5350
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4464 4648 1305
Size: 3159 184 2007
Fragments arranged by size:
3159 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2418 10
Size: 54 1425 343 586 2942
Fragments arranged by size:
2942 1425 586 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2013 2528 3308 3667 3826 518
Size: 648 847 515 780 359 159 2042
Fragments arranged by size:
2042 847 780 648 515 359 159
MspA1I CmG'CkG
Cuts at: 1606 2766 3443 4591 4836 1606
Size: 1160 677 1148 245 2120
Fragments arranged by size:
2120 1160 1148 677 245
NarI GG'CG_CC
Cuts at: 2643 2643
Size: 5350
NcoI C'CATG_G
Cuts at: 513 1065 2419 513
Size: 552 1354 3444
Fragments arranged by size:
3444 1354 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5350
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5350
NotI GC'GGCC_GC
Cuts at: 2797 2797
Size: 5350
NspI r_CATG'y
Cuts at: 1132 1229 2708 4253 1132
Size: 97 1479 1545 2229
Fragments arranged by size:
2229 1545 1479 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5350
PciI A'CATG_T
Cuts at: 4249 4249
Size: 5350
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5350
PmeI GTTT'AAAC
Cuts at: 2654 2654
Size: 5350
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2225 2257 3419 3451 4932 4964 2225
Size: 32 1162 32 1481 32 2611
Fragments arranged by size:
2611 1481 1162 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2440 2440
Size: 5350
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5350
PsiI TTA'TAA
Cuts at: 2920 2920
Size: 5350
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2192 2224 2192
Size: 32 5318
PstI C_TGCA'G
Cuts at: 830 2439 2702 830
Size: 1609 263 3478
Fragments arranged by size:
3478 1609 263
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5350
SalI G'TCGA_C
Cuts at: 1936 2692 1936
Size: 756 4594
SapI GCTCTTCn'nnn_
Cuts at: 2445 2445
Size: 5350
SbfI CC_TGCA'GG
Cuts at: 2702 2702
Size: 5350
ScaI AGT'ACT
Cuts at: 3598 5310 3598
Size: 1712 3638
SfcI C'TryA_G
Cuts at: 826 1045 2435 2698 3833 4514 4705 826
Size: 219 1390 263 1135 681 191 1471
Fragments arranged by size:
1471 1390 1135 681 263 219 191
SfoI GGC'GCC
Cuts at: 2644 2644
Size: 5350
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5350
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5350
SmaI CCC'GGG
Cuts at: 1872 2677 1872
Size: 805 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3454 4355 4617 820
Size: 197 926 1511 901 262 1553
Fragments arranged by size:
1553 1511 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3141 493
Size: 2648 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5350
SphI G_CATG'C
Cuts at: 1132 2708 1132
Size: 1576 3774
SspI AAT'ATT
Cuts at: 5 52 3274 5
Size: 47 3222 2081
Fragments arranged by size:
3222 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5350
StyI C'CwwG_G
Cuts at: 513 1065 2419 2646 2789 513
Size: 552 1354 227 143 3074
Fragments arranged by size:
3074 1354 552 227 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2480 2612 3377 3394 3547 3732 4177
Size: 1255 900 132 765 17 153 185 445
Cuts at: 4177 325
Size: 1498
Fragments arranged by size:
1498 1255 900 765 445 185 153 132
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3596 5308 96
Size: 274 80 33 51 1443 1619 1712 138
Fragments arranged by size:
1712 1619 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5310 5342 5310
Size: 32 5318
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5350
XbaI T'CTAG_A
Cuts at: 2686 2686
Size: 5350
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2417 2417
Size: 5350
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5350
XmaI C'CCGG_G
Cuts at: 1870 2675 1870
Size: 805 4545
XmnI GAAnn'nnTTC
Cuts at: 3479 3479
Size: 5350
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3158 276
Size: 53 83 186 936 1624 2468
Fragments arranged by size:
2468 1624 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PvuII RsrII
SacI SacII SanDI SexAI SfiI SrfI SwaI