Restriction map for
pFN21AE5205
(Circular) (Six-base) MAPSORT of: pFN21AE5205.seq Check: 2850 from: 1 to: 5425
pFN21AE5205 5425 bp
With 182 enzymes: SgfI *
December 8, 2014 13:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3235 278
Size: 53 83 186 936 1699 2468
Fragments arranged by size:
2468 1699 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2768 1937
Size: 831 4594
Acc65I G'GTAC_C
Cuts at: 2746 2746
Size: 5425
AclI AA'CG_TT
Cuts at: 1563 3552 3925 1563
Size: 1989 373 3063
Fragments arranged by size:
3063 1989 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2550 3488 4872 5140 1632
Size: 918 938 1384 268 1917
Fragments arranged by size:
1917 1384 938 918 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5228
AflIII A'CryG_T
Cuts at: 1203 4324 5301 1203
Size: 3121 977 1327
Fragments arranged by size:
3121 1327 977
AgeI A'CCGG_T
Cuts at: 4256 4256
Size: 5425
AhdI GACnn_n'nnGTC
Cuts at: 4154 4154
Size: 5425
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5425
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5425
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2036 2068 2036
Size: 32 5393
AlwNI CAG_nnn'CTG
Cuts at: 3168 4740 3168
Size: 1572 3853
ApaI G_GGCC'C
Cuts at: 2183 2183
Size: 5425
ApaLI G'TGCA_C
Cuts at: 3482 4638 3482
Size: 1156 4269
ApoI r'AATT_y
Cuts at: 1493 2734 2960 3112 5221 1493
Size: 1241 226 152 2109 1697
Fragments arranged by size:
2109 1697 1241 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3979 160
Size: 872 2947 1606
Fragments arranged by size:
2947 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5425
AvaI C'yCGr_G
Cuts at: 1870 1943 2750 1870
Size: 73 807 4545
Fragments arranged by size:
4545 807 73
BamHI G'GATC_C
Cuts at: 2755 3122 2755
Size: 367 5058
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2746 4201 618
Size: 325 217 627 959 1455 1842
Fragments arranged by size:
1842 1455 959 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2183 2352 2616 2744 1391
Size: 792 169 264 128 4072
Fragments arranged by size:
4072 792 264 169 128
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4454
BbvCI CC'TCA_GC
Cuts at: 2691 2691
Size: 5425
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3616 3650 1903
Size: 34 1679 34 3678
Fragments arranged by size:
3678 1679 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3317 4527 3317
Size: 1210 4215
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5425
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4036 136
Size: 107 122 71 631 605 2364 1525
Fragments arranged by size:
2364 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5420 5420
Size: 5425
BlpI GC'TnA_GC
Cuts at: 2842 2842
Size: 5425
Bme1580I G_kGCm'C
Cuts at: 1790 2183 3486 4642 1790
Size: 393 1303 1156 2573
Fragments arranged by size:
2573 1303 1156 393
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5425
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3156 4114 442
Size: 416 53 83 399 24 1739 958 1753
Fragments arranged by size:
1753 1739 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5425
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2635 2635
Size: 5425
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2510 4085 1239
Size: 1271 1575 2579
Fragments arranged by size:
2579 1575 1271
Bpu10I CC'TnA_GC
Cuts at: 2691 2691
Size: 5425
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3550 4415 4713 3550
Size: 865 298 4262
Fragments arranged by size:
4262 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4088 882
Size: 617 2589 2219
Fragments arranged by size:
2589 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3216 493
Size: 711 2012 2702
Fragments arranged by size:
2702 2012 711
BsaBI GATnn'nnATC
Cuts at: 5419 5419
Size: 5425
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3232 3614 4249 275
Size: 53 83 186 936 1699 382 635 1451
Fragments arranged by size:
1699 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3858 4256 4530 4677 5088 3858
Size: 398 274 147 411 4195
Fragments arranged by size:
4195 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5200
Fragments arranged by size:
5200 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2485 2552 2669 1186
Size: 1299 67 117 3942
Fragments arranged by size:
3942 1299 117 67
BseYI C'CCAG_C
Cuts at: 4628 5124 4628
Size: 496 4929
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4852
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2875 3636 4664 1540
Size: 275 186 874 761 1028 2301
Fragments arranged by size:
2301 1028 874 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3486 3571 4642 3486
Size: 85 1071 4269
Fragments arranged by size:
4269 1071 85
BsmI GAATG_Cn'
Cuts at: 2171 2392 2936 3029 2171
Size: 221 544 93 4567
Fragments arranged by size:
4567 544 221 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2159 2159
Size: 5425
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2183 2352 2616 2744 3486 3571 4642
Size: 399 393 169 264 128 742 85 1071
Cuts at: 4642 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 393 264 169 128
85
BspEI T'CCGG_A
Cuts at: 5088 5088
Size: 5425
BspHI T'CATG_A
Cuts at: 2713 3314 5044 2713
Size: 601 1730 3094
Fragments arranged by size:
3094 1730 601
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2780 844
Size: 1936 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3312 1394
Size: 429 1489 3507
Fragments arranged by size:
3507 1489 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3920 4094 65
Size: 1183 2672 174 1396
Fragments arranged by size:
2672 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2019 2083 4069 4256 1700
Size: 110 209 64 1986 187 2869
Fragments arranged by size:
2869 1986 209 187 110 64
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5425
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5425
BssSI C'ACGA_G
Cuts at: 2162 3485 4497 2162
Size: 1323 1012 3090
Fragments arranged by size:
3090 1323 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5425
BstBI TT'CG_AA
Cuts at: 4229 4229
Size: 5425
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5425
BstYI r'GATC_y
Cuts at: 1972 2755 3122 3507 3524 4976 5062 5074 5420
Size: 783 367 385 17 1452 86 12 346
Cuts at: 5420 1972
Size: 1977
Fragments arranged by size:
1977 1452 783 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2106 2106
Size: 5425
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4873
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4686
Fragments arranged by size:
4686 683 56
BtsI GCAGTG_nn'
Cuts at: 2941 3735 3755 2941
Size: 794 20 4611
Fragments arranged by size:
4611 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5425
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5390
DraI TTT'AAA
Cuts at: 2729 2729
Size: 5425
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4432 809
Size: 507 3116 1802
Fragments arranged by size:
3116 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2081 2623 2872
Size: 54 1425 50 275 18 251 542 249
Cuts at: 2872 3761 5390 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 542 275 251 249 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2872 1537
Size: 275 1060 4090
Fragments arranged by size:
4090 1060 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2198 2328 2462 2646 2658 2664 3355 1353
Size: 845 130 134 184 12 6 691 3423
Fragments arranged by size:
3423 845 691 184 134 130 12 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4012 4386 4532 387
Size: 1064 138 2423 374 146 1280
Fragments arranged by size:
2423 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2510 2550 3488 4085 4872 5140 1239
Size: 393 878 40 938 597 787 268 1524
Fragments arranged by size:
1524 938 878 787 597 393 268 40
EcoO109I rG'GnC_Cy
Cuts at: 2044 2157 2180 2571 2630 2695 2044
Size: 113 23 391 59 65 4774
Fragments arranged by size:
4774 391 113 65 59 23
EcoRI G'AATT_C
Cuts at: 2734 3112 2734
Size: 378 5047
FseI GG_CCGG'CC
Cuts at: 2087 2087
Size: 5425
FspI TGC'GCA
Cuts at: 3931 3931
Size: 5425
HaeII r_GCGC'y
Cuts at: 1678 4572 1678
Size: 2894 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4066 4098 4140 4172 1373
Size: 32 135 32 2494 32 42 32 2626
Fragments arranged by size:
2626 2494 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2769 3015 669
Size: 988 281 831 246 3079
Fragments arranged by size:
3079 988 831 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5425
HpaI GTT'AAC
Cuts at: 3015 3015
Size: 5425
KpnI G_GTAC'C
Cuts at: 2750 2750
Size: 5425
MfeI C'AATT_G
Cuts at: 3024 3024
Size: 5425
MluI A'CGCG_T
Cuts at: 5301 5301
Size: 5425
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2040 4539 4723 1305
Size: 735 2499 184 2007
Fragments arranged by size:
2499 2007 735 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3603
Fragments arranged by size:
3603 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3383 3742 3901 518
Size: 648 2217 359 159 2042
Fragments arranged by size:
2217 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2841 3518 4666 4911 1606
Size: 1235 677 1148 245 2120
Fragments arranged by size:
2120 1235 1148 677 245
NaeI GCC'GGC
Cuts at: 2085 2085
Size: 5425
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4873
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5425
NgoMIV G'CCGG_C
Cuts at: 2083 2083
Size: 5425
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5425
NotI GC'GGCC_GC
Cuts at: 2872 2872
Size: 5425
NruI TCG'CGA
Cuts at: 2011 2011
Size: 5425
NspI r_CATG'y
Cuts at: 1132 1229 2783 4328 1132
Size: 97 1554 1545 2229
Fragments arranged by size:
2229 1554 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5425
PciI A'CATG_T
Cuts at: 4324 4324
Size: 5425
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5425
PmeI GTTT'AAAC
Cuts at: 2729 2729
Size: 5425
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3494 3526 5007 5039 3494
Size: 32 1481 32 3880
Fragments arranged by size:
3880 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2044 2157 2044
Size: 113 5312
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5425
PsiI TTA'TAA
Cuts at: 2995 2995
Size: 5425
PspOMI G'GGCC_C
Cuts at: 2179 2179
Size: 5425
PstI C_TGCA'G
Cuts at: 830 2362 2777 830
Size: 1532 415 3478
Fragments arranged by size:
3478 1532 415
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5425
SalI G'TCGA_C
Cuts at: 1936 2767 1936
Size: 831 4594
SanDI GG'GwC_CC
Cuts at: 2157 2157
Size: 5425
SapI GCTCTTCn'nnn_
Cuts at: 2328 2328
Size: 5425
SbfI CC_TGCA'GG
Cuts at: 2777 2777
Size: 5425
ScaI AGT'ACT
Cuts at: 3673 5385 3673
Size: 1712 3713
SexAI A'CCwGG_T
Cuts at: 2046 2046
Size: 5425
SfcI C'TryA_G
Cuts at: 826 1045 2358 2773 3908 4589 4780 826
Size: 219 1313 415 1135 681 191 1471
Fragments arranged by size:
1471 1313 1135 681 415 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5425
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5425
SmaI CCC'GGG
Cuts at: 1872 2752 1872
Size: 880 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3529 4430 4692 820
Size: 197 926 1586 901 262 1553
Fragments arranged by size:
1586 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3216 493
Size: 2723 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5425
SphI G_CATG'C
Cuts at: 1132 2783 1132
Size: 1651 3774
SspI AAT'ATT
Cuts at: 5 52 3349 5
Size: 47 3297 2081
Fragments arranged by size:
3297 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5425
StyI C'CwwG_G
Cuts at: 513 1065 2211 2439 2511 2556 2679 2864 513
Size: 552 1146 228 72 45 123 185 3074
Fragments arranged by size:
3074 1146 552 228 185 123 72 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3452 3469 3622 3807 4252 325
Size: 1255 1872 17 153 185 445 1498
Fragments arranged by size:
1872 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2031 3671 5383
Size: 274 80 33 51 1443 54 1640 1712
Cuts at: 5383 96
Size: 138
Fragments arranged by size:
1712 1640 1443 274 138 80 54 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5385 5417 5385
Size: 32 5393
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5425
XbaI T'CTAG_A
Cuts at: 2761 2761
Size: 5425
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5425
XmaI C'CCGG_G
Cuts at: 1870 2750 1870
Size: 880 4545
XmnI GAAnn'nnTTC
Cuts at: 2246 3554 2246
Size: 1308 4117
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3233 276
Size: 53 83 186 936 1699 2468
Fragments arranged by size:
2468 1699 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NruI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SalI SanDI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BfrBI BsiWI
BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FspAI
KasI NarI NsiI PacI PfoI PmlI PspXI PsrI
PvuII RsrII SacI SacII SfiI SfoI SrfI SwaI
XcmI