Restriction map for
pFN21AE5183
(Circular) (Six-base) MAPSORT of: pFN21AE5183.seq Check: 1502 from: 1 to: 5050
pFN21AE5183 5050 bp
With 182 enzymes: SgfI *
January 30, 2014 17:14 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2166 2860 278
Size: 53 83 186 936 630 694 2468
Fragments arranged by size:
2468 936 694 630 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2393 1937
Size: 456 4594
Acc65I G'GTAC_C
Cuts at: 2371 2371
Size: 5050
AclI AA'CG_TT
Cuts at: 1563 3177 3550 1563
Size: 1614 373 3063
Fragments arranged by size:
3063 1614 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2331 3113 4497 4765 1632
Size: 699 782 1384 268 1917
Fragments arranged by size:
1917 1384 782 699 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4853
AflIII A'CryG_T
Cuts at: 1203 3949 4926 1203
Size: 2746 977 1327
Fragments arranged by size:
2746 1327 977
AgeI A'CCGG_T
Cuts at: 3881 3881
Size: 5050
AhdI GACnn_n'nnGTC
Cuts at: 3779 3779
Size: 5050
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5050
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5050
AlwNI CAG_nnn'CTG
Cuts at: 2240 2249 2793 4365 2240
Size: 9 544 1572 2925
Fragments arranged by size:
2925 1572 544 9
ApaLI G'TGCA_C
Cuts at: 3107 4263 3107
Size: 1156 3894
ApoI r'AATT_y
Cuts at: 1493 2359 2585 2737 4846 1493
Size: 866 226 152 2109 1697
Fragments arranged by size:
2109 1697 866 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3604 160
Size: 872 2572 1606
Fragments arranged by size:
2572 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5050
AvaI C'yCGr_G
Cuts at: 1870 1943 2375 1870
Size: 73 432 4545
Fragments arranged by size:
4545 432 73
AvrII C'CTAG_G
Cuts at: 2038 2038
Size: 5050
BamHI G'GATC_C
Cuts at: 2380 2747 2380
Size: 367 4683
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2371 3826 618
Size: 325 217 627 584 1455 1842
Fragments arranged by size:
1842 1455 627 584 325 217
BanII G_rGCy'C
Cuts at: 1391 2369 1391
Size: 978 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2166 928
Size: 971 267 3812
Fragments arranged by size:
3812 971 267
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3241 3275 1903
Size: 34 50 34 1220 34 3678
Fragments arranged by size:
3678 1220 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2942 4152 2942
Size: 1210 3840
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5050
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3661 136
Size: 107 122 71 631 605 1989 1525
Fragments arranged by size:
1989 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2079 5045 2079
Size: 2966 2084
BlpI GC'TnA_GC
Cuts at: 2467 2467
Size: 5050
Bme1580I G_kGCm'C
Cuts at: 1790 3111 4267 1790
Size: 1321 1156 2573
Fragments arranged by size:
2573 1321 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5050
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2781 3739 442
Size: 416 53 83 399 24 1364 958 1753
Fragments arranged by size:
1753 1364 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5050
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3710 1239
Size: 2471 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3175 4040 4338 3175
Size: 865 298 3887
Fragments arranged by size:
3887 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3713 882
Size: 617 2214 2219
Fragments arranged by size:
2219 2214 617
BsaAI yAC'GTr
Cuts at: 493 1204 2841 493
Size: 711 1637 2702
Fragments arranged by size:
2702 1637 711
BsaBI GATnn'nnATC
Cuts at: 5044 5044
Size: 5050
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2163 2857 3239 3874
Size: 53 83 186 936 630 694 382 635
Cuts at: 3874 275
Size: 1451
Fragments arranged by size:
1451 936 694 635 630 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3483 3881 4155 4302 4713 3483
Size: 398 274 147 411 3820
Fragments arranged by size:
3820 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2003 2033 1178
Size: 30 165 30 600 30 4195
Fragments arranged by size:
4195 600 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2041 2294 2318 1186
Size: 855 253 24 3918
Fragments arranged by size:
3918 855 253 24
BseYI C'CCAG_C
Cuts at: 2057 4253 4749 2057
Size: 2196 496 2358
Fragments arranged by size:
2358 2196 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4477
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2116 2500 3261 4289 1540
Size: 275 186 115 384 761 1028 2301
Fragments arranged by size:
2301 1028 761 384 275 186 115
BsiHKAI G_wGCw'C
Cuts at: 3111 3196 4267 3111
Size: 85 1071 3894
Fragments arranged by size:
3894 1071 85
BsmI GAATG_Cn'
Cuts at: 2561 2654 2561
Size: 93 4957
BsmBI CGTCTCn'nnnn_
Cuts at: 2227 2227
Size: 5050
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2369 3111 3196 4267 1391
Size: 399 579 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 579 399 85
BspEI T'CCGG_A
Cuts at: 4713 4713
Size: 5050
BspHI T'CATG_A
Cuts at: 2939 4669 2939
Size: 1730 3320
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2034 2209 2405 844
Size: 1190 175 196 3489
Fragments arranged by size:
3489 1190 196 175
BsrBI CCG'CTC
Cuts at: 1394 1823 2937 1394
Size: 429 1114 3507
Fragments arranged by size:
3507 1114 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2166 3545 3719 65
Size: 1183 918 1379 174 1396
Fragments arranged by size:
1396 1379 1183 918 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2115 3694 3881 1700
Size: 110 305 1579 187 2869
Fragments arranged by size:
2869 1579 305 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5050
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5050
BssSI C'ACGA_G
Cuts at: 3110 4122 3110
Size: 1012 4038
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2249 794
Size: 1455 3595
BstBI TT'CG_AA
Cuts at: 3854 3854
Size: 5050
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5050
BstXI CCAn_nnnn'nTGG
Cuts at: 2180 2180
Size: 5050
BstYI r'GATC_y
Cuts at: 1972 2079 2380 2747 3132 3149 4601 4687 4699
Size: 107 301 367 385 17 1452 86 12
Cuts at: 4699 5045 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 301 107 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4498
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4311
Fragments arranged by size:
4311 683 56
BtsI GCAGTG_nn'
Cuts at: 2566 3360 3380 2566
Size: 794 20 4236
Fragments arranged by size:
4236 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5050
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5015
DraI TTT'AAA
Cuts at: 2354 2354
Size: 5050
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4057 809
Size: 507 2741 1802
Fragments arranged by size:
2741 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2497 3386 5015
Size: 54 1425 50 275 18 667 889 1629
Cuts at: 5015 8
Size: 43
Fragments arranged by size:
1629 1425 889 667 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2497 1537
Size: 275 685 4090
Fragments arranged by size:
4090 685 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2307 2980 1353
Size: 954 673 3423
Fragments arranged by size:
3423 954 673
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2120 3637 4011 4157 387
Size: 1064 138 531 1517 374 146 1280
Fragments arranged by size:
1517 1280 1064 531 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2331 3113 3710 4497 4765 1239
Size: 393 699 782 597 787 268 1524
Fragments arranged by size:
1524 787 782 699 597 393 268
EcoRI G'AATT_C
Cuts at: 2359 2737 2359
Size: 378 4672
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2098 2098
Size: 5050
FspI TGC'GCA
Cuts at: 3556 3556
Size: 5050
HaeII r_GCGC'y
Cuts at: 1678 2064 4197 1678
Size: 386 2133 2531
Fragments arranged by size:
2531 2133 386
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3691 3723 3765 3797 1373
Size: 32 135 32 2119 32 42 32 2626
Fragments arranged by size:
2626 2119 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2394 2640 669
Size: 988 281 456 246 3079
Fragments arranged by size:
3079 988 456 281 246
HindIII A'AGCT_T
Cuts at: 748 2186 748
Size: 1438 3612
HpaI GTT'AAC
Cuts at: 2640 2640
Size: 5050
KpnI G_GTAC'C
Cuts at: 2375 2375
Size: 5050
MfeI C'AATT_G
Cuts at: 2649 2649
Size: 5050
MluI A'CGCG_T
Cuts at: 4926 4926
Size: 5050
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4164 4348 1305
Size: 2859 184 2007
Fragments arranged by size:
2859 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3228
Fragments arranged by size:
3228 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3008 3367 3526 518
Size: 648 1842 359 159 2042
Fragments arranged by size:
2042 1842 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2466 3143 4291 4536 1606
Size: 860 677 1148 245 2120
Fragments arranged by size:
2120 1148 860 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4498
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5050
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5050
NotI GC'GGCC_GC
Cuts at: 2497 2497
Size: 5050
NspI r_CATG'y
Cuts at: 1132 1229 2408 3953 1132
Size: 97 1179 1545 2229
Fragments arranged by size:
2229 1545 1179 97
PasI CC'CwG_GG
Cuts at: 1348 2173 1348
Size: 825 4225
PciI A'CATG_T
Cuts at: 3949 3949
Size: 5050
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5050
PmeI GTTT'AAAC
Cuts at: 2354 2354
Size: 5050
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3119 3151 4632 4664 3119
Size: 32 1481 32 3505
Fragments arranged by size:
3505 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5050
PsiI TTA'TAA
Cuts at: 2620 2620
Size: 5050
PstI C_TGCA'G
Cuts at: 830 2245 2275 2402 830
Size: 1415 30 127 3478
Fragments arranged by size:
3478 1415 127 30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5050
SalI G'TCGA_C
Cuts at: 1936 2392 1936
Size: 456 4594
SbfI CC_TGCA'GG
Cuts at: 2402 2402
Size: 5050
ScaI AGT'ACT
Cuts at: 3298 5010 3298
Size: 1712 3338
SfcI C'TryA_G
Cuts at: 826 1045 2214 2241 2271 2398 3533 4214 4405
Size: 219 1169 27 30 127 1135 681 191
Cuts at: 4405 826
Size: 1471
Fragments arranged by size:
1471 1169 1135 681 219 191 127 30
27
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5050
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5050
SmaI CCC'GGG
Cuts at: 1872 2377 1872
Size: 505 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3154 4055 4317 820
Size: 197 926 1211 901 262 1553
Fragments arranged by size:
1553 1211 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2841 493
Size: 2348 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5050
SphI G_CATG'C
Cuts at: 1132 2408 1132
Size: 1276 3774
SspI AAT'ATT
Cuts at: 5 52 2974 5
Size: 47 2922 2081
Fragments arranged by size:
2922 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5050
StyI C'CwwG_G
Cuts at: 513 1065 2038 2064 2489 513
Size: 552 973 26 425 3074
Fragments arranged by size:
3074 973 552 425 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3077 3094 3247 3432 3877 325
Size: 1255 1497 17 153 185 445 1498
Fragments arranged by size:
1498 1497 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3296 5008 96
Size: 274 80 33 51 1443 1319 1712 138
Fragments arranged by size:
1712 1443 1319 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5010 5042 5010
Size: 32 5018
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5050
XbaI T'CTAG_A
Cuts at: 2386 2386
Size: 5050
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5050
XmaI C'CCGG_G
Cuts at: 1870 2375 1870
Size: 505 4545
XmnI GAAnn'nnTTC
Cuts at: 3179 3179
Size: 5050
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2164 2858 276
Size: 53 83 186 936 630 694 2468
Fragments arranged by size:
2468 936 694 630 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI