Restriction map for
pFN21AE5156
(Circular) (Six-base) MAPSORT of: pFN21AE5156.seq Check: 8605 from: 1 to: 5044
pFN21AE5156 5044 bp
With 182 enzymes: SgfI *
January 30, 2014 17:13 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2854 278
Size: 53 83 186 936 1318 2468
Fragments arranged by size:
2468 1318 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2387 1937
Size: 450 4594
Acc65I G'GTAC_C
Cuts at: 2365 2365
Size: 5044
AclI AA'CG_TT
Cuts at: 1563 3171 3544 1563
Size: 1608 373 3063
Fragments arranged by size:
3063 1608 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3107 4491 4759 1632
Size: 1475 1384 268 1917
Fragments arranged by size:
1917 1475 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4847
AflIII A'CryG_T
Cuts at: 1203 3943 4920 1203
Size: 2740 977 1327
Fragments arranged by size:
2740 1327 977
AgeI A'CCGG_T
Cuts at: 3875 3875
Size: 5044
AhdI GACnn_n'nnGTC
Cuts at: 3773 3773
Size: 5044
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5044
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2147 1184
Size: 963 4081
AlwNI CAG_nnn'CTG
Cuts at: 2787 4359 2787
Size: 1572 3472
ApaLI G'TGCA_C
Cuts at: 3101 4257 3101
Size: 1156 3888
ApoI r'AATT_y
Cuts at: 1493 2353 2579 2731 4840 1493
Size: 860 226 152 2109 1697
Fragments arranged by size:
2109 1697 860 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3598 160
Size: 872 2566 1606
Fragments arranged by size:
2566 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5044
AvaI C'yCGr_G
Cuts at: 1870 1943 2369 1870
Size: 73 426 4545
Fragments arranged by size:
4545 426 73
BamHI G'GATC_C
Cuts at: 2241 2374 2741 2241
Size: 133 367 4544
Fragments arranged by size:
4544 367 133
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2056 2365 3820 618
Size: 325 217 627 269 309 1455 1842
Fragments arranged by size:
1842 1455 627 325 309 269 217
BanII G_rGCy'C
Cuts at: 1391 2145 2363 1391
Size: 754 218 4072
Fragments arranged by size:
4072 754 218
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4073
BbvCI CC'TCA_GC
Cuts at: 2217 2217
Size: 5044
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3235 3269 1903
Size: 34 1298 34 3678
Fragments arranged by size:
3678 1298 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2936 4146 2936
Size: 1210 3834
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5044
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3655 136
Size: 107 122 71 631 605 1983 1525
Fragments arranged by size:
1983 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5039 5039
Size: 5044
BlpI GC'TnA_GC
Cuts at: 2461 2461
Size: 5044
Bme1580I G_kGCm'C
Cuts at: 1790 3105 4261 1790
Size: 1315 1156 2573
Fragments arranged by size:
2573 1315 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5044
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2007 2775 3733
Size: 416 53 83 399 24 590 768 958
Cuts at: 3733 442
Size: 1753
Fragments arranged by size:
1753 958 768 590 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5044
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3704 1239
Size: 2465 2579
Bpu10I CC'TnA_GC
Cuts at: 2217 2217
Size: 5044
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3169 4034 4332 3169
Size: 865 298 3881
Fragments arranged by size:
3881 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2080 2161 3707 882
Size: 617 581 81 1546 2219
Fragments arranged by size:
2219 1546 617 581 81
BsaAI yAC'GTr
Cuts at: 493 1204 2835 493
Size: 711 1631 2702
Fragments arranged by size:
2702 1631 711
BsaBI GATnn'nnATC
Cuts at: 5038 5038
Size: 5044
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2851 3233 3868 275
Size: 53 83 186 936 1318 382 635 1451
Fragments arranged by size:
1451 1318 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3477 3875 4149 4296 4707 3477
Size: 398 274 147 411 3814
Fragments arranged by size:
3814 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2033 2063 1178
Size: 30 165 30 630 30 4159
Fragments arranged by size:
4159 630 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2225 1186
Size: 1039 4005
BseYI C'CCAG_C
Cuts at: 2018 4247 4743 2018
Size: 2229 496 2319
Fragments arranged by size:
2319 2229 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2153 1163
Size: 573 417 4054
Fragments arranged by size:
4054 573 417
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2494 3255 4283 1540
Size: 275 186 493 761 1028 2301
Fragments arranged by size:
2301 1028 761 493 275 186
BsiHKAI G_wGCw'C
Cuts at: 2308 3105 3190 4261 2308
Size: 797 85 1071 3091
Fragments arranged by size:
3091 1071 797 85
BsmI GAATG_Cn'
Cuts at: 2555 2648 2555
Size: 93 4951
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2145 2308 2363 3105 3190 4261 1391
Size: 399 355 163 55 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 355 163 85 55
BspEI T'CCGG_A
Cuts at: 4707 4707
Size: 5044
BspHI T'CATG_A
Cuts at: 2118 2933 4663 2118
Size: 815 1730 2499
Fragments arranged by size:
2499 1730 815
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2399 844
Size: 1555 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2931 1394
Size: 429 1108 3507
Fragments arranged by size:
3507 1108 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3539 3713 65
Size: 1183 2291 174 1396
Fragments arranged by size:
2291 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2232 3688 3875 1700
Size: 110 422 1456 187 2869
Fragments arranged by size:
2869 1456 422 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5044
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5044
BssSI C'ACGA_G
Cuts at: 3104 4116 3104
Size: 1012 4032
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5044
BstBI TT'CG_AA
Cuts at: 3848 3848
Size: 5044
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5044
BstXI CCAn_nnnn'nTGG
Cuts at: 2152 2152
Size: 5044
BstYI r'GATC_y
Cuts at: 1972 2241 2374 2741 3126 3143 4595 4681 4693
Size: 269 133 367 385 17 1452 86 12
Cuts at: 4693 5039 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 269 133 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4492
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2277 487
Size: 683 56 1051 3254
Fragments arranged by size:
3254 1051 683 56
BtsI GCAGTG_nn'
Cuts at: 2318 2560 3354 3374 2318
Size: 242 794 20 3988
Fragments arranged by size:
3988 794 242 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5044
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5009
DraI TTT'AAA
Cuts at: 2348 2348
Size: 5044
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4051 809
Size: 507 2735 1802
Fragments arranged by size:
2735 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2491 3380 5009
Size: 54 1425 50 275 18 661 889 1629
Cuts at: 5009 8
Size: 43
Fragments arranged by size:
1629 1425 889 661 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2491 1537
Size: 275 679 4090
Fragments arranged by size:
4090 679 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2974 1353
Size: 1621 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3631 4005 4151 387
Size: 1064 138 2042 374 146 1280
Fragments arranged by size:
2042 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3107 3704 4491 4759 1239
Size: 393 1475 597 787 268 1524
Fragments arranged by size:
1524 1475 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2353 2731 2353
Size: 378 4666
FspI TGC'GCA
Cuts at: 3550 3550
Size: 5044
HaeII r_GCGC'y
Cuts at: 1678 2203 4191 1678
Size: 525 1988 2531
Fragments arranged by size:
2531 1988 525
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2033 2065 3685 3717 3759
Size: 32 135 32 461 32 1620 32 42
Cuts at: 3759 3791 1373
Size: 32 2626
Fragments arranged by size:
2626 1620 461 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2388 2634 669
Size: 988 281 450 246 3079
Fragments arranged by size:
3079 988 450 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5044
HpaI GTT'AAC
Cuts at: 2634 2634
Size: 5044
KpnI G_GTAC'C
Cuts at: 2369 2369
Size: 5044
MfeI C'AATT_G
Cuts at: 2643 2643
Size: 5044
MluI A'CGCG_T
Cuts at: 4920 4920
Size: 5044
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4158 4342 1305
Size: 2853 184 2007
Fragments arranged by size:
2853 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3222
Fragments arranged by size:
3222 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2129 3002 3361 3520 518
Size: 648 963 873 359 159 2042
Fragments arranged by size:
2042 963 873 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2197 2460 3137 4285 4530 1606
Size: 591 263 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 591 263 245
NaeI GCC'GGC
Cuts at: 2234 2234
Size: 5044
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4492
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5044
NgoMIV G'CCGG_C
Cuts at: 2232 2232
Size: 5044
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5044
NotI GC'GGCC_GC
Cuts at: 2491 2491
Size: 5044
NspI r_CATG'y
Cuts at: 1132 1229 2402 3947 1132
Size: 97 1173 1545 2229
Fragments arranged by size:
2229 1545 1173 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5044
PciI A'CATG_T
Cuts at: 3943 3943
Size: 5044
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5044
PmeI GTTT'AAAC
Cuts at: 2348 2348
Size: 5044
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3113 3145 4626 4658 3113
Size: 32 1481 32 3499
Fragments arranged by size:
3499 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5044
PsiI TTA'TAA
Cuts at: 2614 2614
Size: 5044
PstI C_TGCA'G
Cuts at: 830 2254 2396 830
Size: 1424 142 3478
Fragments arranged by size:
3478 1424 142
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5044
SalI G'TCGA_C
Cuts at: 1936 2386 1936
Size: 450 4594
SbfI CC_TGCA'GG
Cuts at: 2396 2396
Size: 5044
ScaI AGT'ACT
Cuts at: 3292 5004 3292
Size: 1712 3332
SfcI C'TryA_G
Cuts at: 826 1045 2250 2392 3527 4208 4399 826
Size: 219 1205 142 1135 681 191 1471
Fragments arranged by size:
1471 1205 1135 681 219 191 142
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5044
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5044
SmaI CCC'GGG
Cuts at: 1872 2371 1872
Size: 499 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3148 4049 4311 820
Size: 197 926 1205 901 262 1553
Fragments arranged by size:
1553 1205 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2835 493
Size: 2342 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5044
SphI G_CATG'C
Cuts at: 1132 2402 1132
Size: 1270 3774
SspI AAT'ATT
Cuts at: 5 52 2968 5
Size: 47 2916 2081
Fragments arranged by size:
2916 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5044
StyI C'CwwG_G
Cuts at: 513 1065 2052 2483 513
Size: 552 987 431 3074
Fragments arranged by size:
3074 987 552 431
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3071 3088 3241 3426 3871 325
Size: 1255 1491 17 153 185 445 1498
Fragments arranged by size:
1498 1491 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3290 5002 96
Size: 274 80 33 51 1443 1313 1712 138
Fragments arranged by size:
1712 1443 1313 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5004 5036 5004
Size: 32 5012
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5044
XbaI T'CTAG_A
Cuts at: 2380 2380
Size: 5044
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5044
XmaI C'CCGG_G
Cuts at: 1870 2369 1870
Size: 499 4545
XmnI GAAnn'nnTTC
Cuts at: 3173 3173
Size: 5044
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2852 276
Size: 53 83 186 936 1318 2468
Fragments arranged by size:
2468 1318 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI