Restriction map for
pFN21AE5149
(Circular) (Six-base) MAPSORT of: pFN21AE5149.seq Check: 9322 from: 1 to: 4981
pFN21AE5149 4981 bp
With 182 enzymes: SgfI *
December 8, 2014 13:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2791 278
Size: 53 83 186 936 1255 2468
Fragments arranged by size:
2468 1255 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2324 1937
Size: 387 4594
Acc65I G'GTAC_C
Cuts at: 2302 2302
Size: 4981
AclI AA'CG_TT
Cuts at: 1563 3108 3481 1563
Size: 1545 373 3063
Fragments arranged by size:
3063 1545 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2282 3044 4428 4696 1632
Size: 650 762 1384 268 1917
Fragments arranged by size:
1917 1384 762 650 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4784
AflIII A'CryG_T
Cuts at: 1203 3880 4857 1203
Size: 2677 977 1327
Fragments arranged by size:
2677 1327 977
AgeI A'CCGG_T
Cuts at: 3812 3812
Size: 4981
AhdI GACnn_n'nnGTC
Cuts at: 3710 3710
Size: 4981
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4981
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4981
AlwNI CAG_nnn'CTG
Cuts at: 2198 2724 4296 2198
Size: 526 1572 2883
Fragments arranged by size:
2883 1572 526
ApaLI G'TGCA_C
Cuts at: 3038 4194 3038
Size: 1156 3825
ApoI r'AATT_y
Cuts at: 1493 2290 2516 2668 4777 1493
Size: 797 226 152 2109 1697
Fragments arranged by size:
2109 1697 797 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3535 160
Size: 872 2503 1606
Fragments arranged by size:
2503 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4981
AvaI C'yCGr_G
Cuts at: 1870 1943 2306 1870
Size: 73 363 4545
Fragments arranged by size:
4545 363 73
BamHI G'GATC_C
Cuts at: 2311 2678 2311
Size: 367 4614
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2204 2302 3757 618
Size: 325 217 627 417 98 1455 1842
Fragments arranged by size:
1842 1455 627 417 325 217 98
BanII G_rGCy'C
Cuts at: 1391 2300 1391
Size: 909 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2195 2263 928
Size: 971 296 68 3646
Fragments arranged by size:
3646 971 296 68
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3172 3206 1903
Size: 34 1235 34 3678
Fragments arranged by size:
3678 1235 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2873 4083 2873
Size: 1210 3771
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4981
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3592 136
Size: 107 122 71 631 605 1920 1525
Fragments arranged by size:
1920 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4976 4976
Size: 4981
BlpI GC'TnA_GC
Cuts at: 2398 2398
Size: 4981
Bme1580I G_kGCm'C
Cuts at: 1790 3042 4198 1790
Size: 1252 1156 2573
Fragments arranged by size:
2573 1252 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4981
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2712 3670 442
Size: 416 53 83 399 24 1295 958 1753
Fragments arranged by size:
1753 1295 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4981
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3641 1239
Size: 2402 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3106 3971 4269 3106
Size: 865 298 3818
Fragments arranged by size:
3818 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3644 882
Size: 617 2145 2219
Fragments arranged by size:
2219 2145 617
BsaAI yAC'GTr
Cuts at: 493 1204 2772 493
Size: 711 1568 2702
Fragments arranged by size:
2702 1568 711
BsaBI GATnn'nnATC
Cuts at: 4975 4975
Size: 4981
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2788 3170 3805 275
Size: 53 83 186 936 1255 382 635 1451
Fragments arranged by size:
1451 1255 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2070 3414 3812 4086 4233 4644 2070
Size: 1344 398 274 147 411 2407
Fragments arranged by size:
2407 1344 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4756
Fragments arranged by size:
4756 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4981
BseYI C'CCAG_C
Cuts at: 4184 4680 4184
Size: 496 4485
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4408
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2431 3192 4220 1540
Size: 275 186 430 761 1028 2301
Fragments arranged by size:
2301 1028 761 430 275 186
BsiHKAI G_wGCw'C
Cuts at: 3042 3127 4198 3042
Size: 85 1071 3825
Fragments arranged by size:
3825 1071 85
BsmI GAATG_Cn'
Cuts at: 2492 2585 2492
Size: 93 4888
BsmBI CGTCTCn'nnnn_
Cuts at: 2143 2143
Size: 4981
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2300 3042 3127 4198 1391
Size: 399 510 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 510 399 85
BspEI T'CCGG_A
Cuts at: 2070 4644 2070
Size: 2574 2407
BspHI T'CATG_A
Cuts at: 2870 4600 2870
Size: 1730 3251
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2336 844
Size: 1492 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2868 1394
Size: 429 1045 3507
Fragments arranged by size:
3507 1045 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3476 3650 65
Size: 1183 2228 174 1396
Fragments arranged by size:
2228 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3625 3812 1700
Size: 110 1815 187 2869
Fragments arranged by size:
2869 1815 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4981
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4981
BssSI C'ACGA_G
Cuts at: 3041 4053 3041
Size: 1012 3969
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4981
BstBI TT'CG_AA
Cuts at: 3785 3785
Size: 4981
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4981
BstYI r'GATC_y
Cuts at: 1972 2163 2311 2678 3063 3080 4532 4618 4630
Size: 191 148 367 385 17 1452 86 12
Cuts at: 4630 4976 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 191 148 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3490
Fragments arranged by size:
3490 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4242
Fragments arranged by size:
4242 683 56
BtsI GCAGTG_nn'
Cuts at: 2497 3291 3311 2497
Size: 794 20 4167
Fragments arranged by size:
4167 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4981
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4946
DraI TTT'AAA
Cuts at: 2285 2285
Size: 4981
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3988 809
Size: 507 2672 1802
Fragments arranged by size:
2672 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2428 3317
Size: 54 1425 50 275 18 177 421 889
Cuts at: 3317 4946 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 421 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2428 1537
Size: 275 616 4090
Fragments arranged by size:
4090 616 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2141 2911 1353
Size: 788 770 3423
Fragments arranged by size:
3423 788 770
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2236 3568 3942 4088 387
Size: 1064 138 647 1332 374 146 1280
Fragments arranged by size:
1332 1280 1064 647 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2282 3044 3641 4428 4696 1239
Size: 393 650 762 597 787 268 1524
Fragments arranged by size:
1524 787 762 650 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2049 2061 2049
Size: 12 4969
EcoRI G'AATT_C
Cuts at: 2290 2668 2290
Size: 378 4603
FspI TGC'GCA
Cuts at: 3487 3487
Size: 4981
HaeII r_GCGC'y
Cuts at: 1678 4128 1678
Size: 2450 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3622 3654 3696 3728 1373
Size: 32 135 32 2050 32 42 32 2626
Fragments arranged by size:
2626 2050 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2325 2571 669
Size: 988 281 387 246 3079
Fragments arranged by size:
3079 988 387 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4981
HpaI GTT'AAC
Cuts at: 2571 2571
Size: 4981
KpnI G_GTAC'C
Cuts at: 2306 2306
Size: 4981
MfeI C'AATT_G
Cuts at: 2580 2580
Size: 4981
MluI A'CGCG_T
Cuts at: 4857 4857
Size: 4981
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4095 4279 1305
Size: 2790 184 2007
Fragments arranged by size:
2790 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 2982
Fragments arranged by size:
2982 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2939 3298 3457 518
Size: 648 1773 359 159 2042
Fragments arranged by size:
2042 1773 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2231 2397 3074 4222 4467 1606
Size: 625 166 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 625 245 166
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3490
Fragments arranged by size:
3490 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4981
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4981
NotI GC'GGCC_GC
Cuts at: 2428 2428
Size: 4981
NspI r_CATG'y
Cuts at: 1132 1229 2339 3884 1132
Size: 97 1110 1545 2229
Fragments arranged by size:
2229 1545 1110 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4981
PciI A'CATG_T
Cuts at: 3880 3880
Size: 4981
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4981
PmeI GTTT'AAAC
Cuts at: 2285 2285
Size: 4981
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3050 3082 4563 4595 3050
Size: 32 1481 32 3436
Fragments arranged by size:
3436 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2049 2049
Size: 4981
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4981
PsiI TTA'TAA
Cuts at: 2551 2551
Size: 4981
PstI C_TGCA'G
Cuts at: 830 2333 830
Size: 1503 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4981
SalI G'TCGA_C
Cuts at: 1936 2323 1936
Size: 387 4594
SanDI GG'GwC_CC
Cuts at: 2049 2049
Size: 4981
SbfI CC_TGCA'GG
Cuts at: 2333 2333
Size: 4981
ScaI AGT'ACT
Cuts at: 3229 4941 3229
Size: 1712 3269
SexAI A'CCwGG_T
Cuts at: 2277 2277
Size: 4981
SfcI C'TryA_G
Cuts at: 826 1045 2329 3464 4145 4336 826
Size: 219 1284 1135 681 191 1471
Fragments arranged by size:
1471 1284 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4981
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4981
SmaI CCC'GGG
Cuts at: 1872 2308 1872
Size: 436 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3085 3986 4248 820
Size: 197 926 1142 901 262 1553
Fragments arranged by size:
1553 1142 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2772 493
Size: 2279 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4981
SphI G_CATG'C
Cuts at: 1132 2339 1132
Size: 1207 3774
SspI AAT'ATT
Cuts at: 5 52 2905 5
Size: 47 2853 2081
Fragments arranged by size:
2853 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4981
StyI C'CwwG_G
Cuts at: 513 1065 2004 2420 513
Size: 552 939 416 3074
Fragments arranged by size:
3074 939 552 416
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3008 3025 3178 3363 3808 325
Size: 1255 1428 17 153 185 445 1498
Fragments arranged by size:
1498 1428 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2112 3227 4939
Size: 274 80 33 51 1443 135 1115 1712
Cuts at: 4939 96
Size: 138
Fragments arranged by size:
1712 1443 1115 274 138 135 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2162 2194 4941 4973 2162
Size: 32 2747 32 2170
Fragments arranged by size:
2747 2170 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4981
XbaI T'CTAG_A
Cuts at: 2317 2317
Size: 4981
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4981
XmaI C'CCGG_G
Cuts at: 1870 2306 1870
Size: 436 4545
XmnI GAAnn'nnTTC
Cuts at: 3110 3110
Size: 4981
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2789 276
Size: 53 83 186 936 1255 2468
Fragments arranged by size:
2468 1255 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI
SfiI SfoI SrfI SwaI XcmI