Restriction map for
pFN21AE5141
(Circular) (Six-base) MAPSORT of: pFN21AE5141.seq Check: 2805 from: 1 to: 5032
pFN21AE5141 5032 bp
With 182 enzymes: SgfI *
January 30, 2014 17:13 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2842 278
Size: 53 83 186 936 1306 2468
Fragments arranged by size:
2468 1306 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2375 1937
Size: 438 4594
Acc65I G'GTAC_C
Cuts at: 2353 2353
Size: 5032
AclI AA'CG_TT
Cuts at: 1563 3159 3532 1563
Size: 1596 373 3063
Fragments arranged by size:
3063 1596 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2193 3095 4479 4747 1632
Size: 561 902 1384 268 1917
Fragments arranged by size:
1917 1384 902 561 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4835
AflIII A'CryG_T
Cuts at: 1203 3931 4908 1203
Size: 2728 977 1327
Fragments arranged by size:
2728 1327 977
AgeI A'CCGG_T
Cuts at: 3863 3863
Size: 5032
AhdI GACnn_n'nnGTC
Cuts at: 3761 3761
Size: 5032
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5032
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5032
AlwNI CAG_nnn'CTG
Cuts at: 2775 4347 2775
Size: 1572 3460
ApaLI G'TGCA_C
Cuts at: 3089 4245 3089
Size: 1156 3876
ApoI r'AATT_y
Cuts at: 1493 2079 2341 2567 2719 4828 1493
Size: 586 262 226 152 2109 1697
Fragments arranged by size:
2109 1697 586 262 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3586 160
Size: 872 2554 1606
Fragments arranged by size:
2554 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5032
AvaI C'yCGr_G
Cuts at: 1870 1943 2357 1870
Size: 73 414 4545
Fragments arranged by size:
4545 414 73
BamHI G'GATC_C
Cuts at: 2362 2729 2362
Size: 367 4665
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2353 3808 618
Size: 325 217 627 566 1455 1842
Fragments arranged by size:
1842 1455 627 566 325 217
BanII G_rGCy'C
Cuts at: 1391 2351 1391
Size: 960 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4061
BbvCI CC'TCA_GC
Cuts at: 2014 2014
Size: 5032
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3223 3257 1903
Size: 34 1286 34 3678
Fragments arranged by size:
3678 1286 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2924 4134 2924
Size: 1210 3822
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5032
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3643 136
Size: 107 122 71 631 605 337 1634 1525
Fragments arranged by size:
1634 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5027 5027
Size: 5032
BlpI GC'TnA_GC
Cuts at: 2449 2449
Size: 5032
Bme1580I G_kGCm'C
Cuts at: 1790 3093 4249 1790
Size: 1303 1156 2573
Fragments arranged by size:
2573 1303 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5032
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2763 3721 442
Size: 416 53 83 399 24 1346 958 1753
Fragments arranged by size:
1753 1346 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5032
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3692 1239
Size: 2453 2579
Bpu10I CC'TnA_GC
Cuts at: 2014 2014
Size: 5032
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3157 4022 4320 3157
Size: 865 298 3869
Fragments arranged by size:
3869 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3695 882
Size: 617 2196 2219
Fragments arranged by size:
2219 2196 617
BsaAI yAC'GTr
Cuts at: 493 1204 2823 493
Size: 711 1619 2702
Fragments arranged by size:
2702 1619 711
BsaBI GATnn'nnATC
Cuts at: 5026 5026
Size: 5032
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2096 2839 3221 3856
Size: 53 83 186 936 563 743 382 635
Cuts at: 3856 275
Size: 1451
Fragments arranged by size:
1451 936 743 635 563 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3465 3863 4137 4284 4695 3465
Size: 398 274 147 411 3802
Fragments arranged by size:
3802 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2253 2283 1178
Size: 30 165 30 850 30 3927
Fragments arranged by size:
3927 850 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5032
BseYI C'CCAG_C
Cuts at: 4235 4731 4235
Size: 496 4536
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4459
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2482 3243 4271 1540
Size: 275 186 481 761 1028 2301
Fragments arranged by size:
2301 1028 761 481 275 186
BsiHKAI G_wGCw'C
Cuts at: 3093 3178 4249 3093
Size: 85 1071 3876
Fragments arranged by size:
3876 1071 85
BsmI GAATG_Cn'
Cuts at: 2233 2543 2636 2233
Size: 310 93 4629
Fragments arranged by size:
4629 310 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2351 3093 3178 4249 1391
Size: 399 561 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 561 399 85
BspEI T'CCGG_A
Cuts at: 4695 4695
Size: 5032
BspHI T'CATG_A
Cuts at: 2921 4651 2921
Size: 1730 3302
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2387 844
Size: 1543 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2919 1394
Size: 429 1096 3507
Fragments arranged by size:
3507 1096 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3527 3701 65
Size: 1183 2279 174 1396
Fragments arranged by size:
2279 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2059 3676 3863 1700
Size: 110 249 1617 187 2869
Fragments arranged by size:
2869 1617 249 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5032
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5032
BssSI C'ACGA_G
Cuts at: 3092 4104 3092
Size: 1012 4020
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5032
BstBI TT'CG_AA
Cuts at: 3836 3836
Size: 5032
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5032
BstYI r'GATC_y
Cuts at: 1972 2362 2729 3114 3131 4583 4669 4681 5027
Size: 390 367 385 17 1452 86 12 346
Cuts at: 5027 1972
Size: 1977
Fragments arranged by size:
1977 1452 390 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3541
Fragments arranged by size:
3541 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4293
Fragments arranged by size:
4293 683 56
BtsI GCAGTG_nn'
Cuts at: 2548 3342 3362 2548
Size: 794 20 4218
Fragments arranged by size:
4218 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5032
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4997
DraI TTT'AAA
Cuts at: 2336 2336
Size: 5032
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2095 4039 809
Size: 507 779 1944 1802
Fragments arranged by size:
1944 1802 779 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2131 2479 3368
Size: 54 1425 50 275 18 301 348 889
Cuts at: 3368 4997 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 348 301 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2479 1537
Size: 275 667 4090
Fragments arranged by size:
4090 667 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2962 1353
Size: 1609 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3619 3993 4139 387
Size: 1064 138 2030 374 146 1280
Fragments arranged by size:
2030 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2193 3095 3692 4479 4747 1239
Size: 393 561 902 597 787 268 1524
Fragments arranged by size:
1524 902 787 597 561 393 268
EcoRI G'AATT_C
Cuts at: 2079 2341 2719 2079
Size: 262 378 4392
Fragments arranged by size:
4392 378 262
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2100 2237 2100
Size: 137 4895
FspI TGC'GCA
Cuts at: 3538 3538
Size: 5032
HaeII r_GCGC'y
Cuts at: 1678 2039 4179 1678
Size: 361 2140 2531
Fragments arranged by size:
2531 2140 361
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2253 2285 3673 3705 3747
Size: 32 135 32 681 32 1388 32 42
Cuts at: 3747 3779 1373
Size: 32 2626
Fragments arranged by size:
2626 1388 681 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2376 2622 669
Size: 988 281 438 246 3079
Fragments arranged by size:
3079 988 438 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5032
HpaI GTT'AAC
Cuts at: 2622 2622
Size: 5032
KpnI G_GTAC'C
Cuts at: 2357 2357
Size: 5032
MfeI C'AATT_G
Cuts at: 2631 2631
Size: 5032
MluI A'CGCG_T
Cuts at: 4908 4908
Size: 5032
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4146 4330 1305
Size: 2841 184 2007
Fragments arranged by size:
2841 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3210
Fragments arranged by size:
3210 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2990 3349 3508 518
Size: 648 1824 359 159 2042
Fragments arranged by size:
2042 1824 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2107 2448 3125 4273 4518 1606
Size: 501 341 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 501 341 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3541
Fragments arranged by size:
3541 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5032
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5032
NotI GC'GGCC_GC
Cuts at: 2479 2479
Size: 5032
NspI r_CATG'y
Cuts at: 1132 1229 2390 3935 1132
Size: 97 1161 1545 2229
Fragments arranged by size:
2229 1545 1161 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5032
PciI A'CATG_T
Cuts at: 3931 3931
Size: 5032
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5032
PmeI GTTT'AAAC
Cuts at: 2336 2336
Size: 5032
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3101 3133 4614 4646 3101
Size: 32 1481 32 3487
Fragments arranged by size:
3487 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5032
PsiI TTA'TAA
Cuts at: 2602 2602
Size: 5032
PstI C_TGCA'G
Cuts at: 830 2384 830
Size: 1554 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5032
PvuII CAG'CTG
Cuts at: 2107 2107
Size: 5032
SalI G'TCGA_C
Cuts at: 1936 2374 1936
Size: 438 4594
SbfI CC_TGCA'GG
Cuts at: 2384 2384
Size: 5032
ScaI AGT'ACT
Cuts at: 3280 4992 3280
Size: 1712 3320
SfcI C'TryA_G
Cuts at: 826 1045 2380 3515 4196 4387 826
Size: 219 1335 1135 681 191 1471
Fragments arranged by size:
1471 1335 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5032
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5032
SmaI CCC'GGG
Cuts at: 1872 2359 1872
Size: 487 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3136 4037 4299 820
Size: 197 926 1193 901 262 1553
Fragments arranged by size:
1553 1193 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2823 493
Size: 2330 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5032
SphI G_CATG'C
Cuts at: 1132 2390 1132
Size: 1258 3774
SspI AAT'ATT
Cuts at: 5 52 2956 5
Size: 47 2904 2081
Fragments arranged by size:
2904 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5032
StyI C'CwwG_G
Cuts at: 513 1065 2004 2084 2471 513
Size: 552 939 80 387 3074
Fragments arranged by size:
3074 939 552 387 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3059 3076 3229 3414 3859 325
Size: 1255 1479 17 153 185 445 1498
Fragments arranged by size:
1498 1479 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3278 4990 96
Size: 274 80 33 51 1443 1301 1712 138
Fragments arranged by size:
1712 1443 1301 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4992 5024 4992
Size: 32 5000
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5032
XbaI T'CTAG_A
Cuts at: 2368 2368
Size: 5032
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5032
XmaI C'CCGG_G
Cuts at: 1870 2357 1870
Size: 487 4545
XmnI GAAnn'nnTTC
Cuts at: 2220 3161 2220
Size: 941 4091
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2840 276
Size: 53 83 186 936 1306 2468
Fragments arranged by size:
2468 1306 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI