Restriction map for
pFN21AE5137
(Circular) (Six-base) MAPSORT of: pFN21AE5137.seq Check: 4220 from: 1 to: 5143
pFN21AE5137 5143 bp
With 182 enzymes: SgfI *
January 30, 2014 17:13 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2953 278
Size: 53 83 186 936 1417 2468
Fragments arranged by size:
2468 1417 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2486 1937
Size: 549 4594
Acc65I G'GTAC_C
Cuts at: 2464 2464
Size: 5143
AclI AA'CG_TT
Cuts at: 1563 3270 3643 1563
Size: 1707 373 3063
Fragments arranged by size:
3063 1707 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2054 3206 4590 4858 1632
Size: 422 1152 1384 268 1917
Fragments arranged by size:
1917 1384 1152 422 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4946
AflIII A'CryG_T
Cuts at: 1203 4042 5019 1203
Size: 2839 977 1327
Fragments arranged by size:
2839 1327 977
AgeI A'CCGG_T
Cuts at: 3974 3974
Size: 5143
AhdI GACnn_n'nnGTC
Cuts at: 3872 3872
Size: 5143
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5143
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5143
AlwNI CAG_nnn'CTG
Cuts at: 2056 2059 2398 2886 4458 2056
Size: 3 339 488 1572 2741
Fragments arranged by size:
2741 1572 488 339 3
ApaLI G'TGCA_C
Cuts at: 3200 4356 3200
Size: 1156 3987
ApoI r'AATT_y
Cuts at: 1493 2452 2678 2830 4939 1493
Size: 959 226 152 2109 1697
Fragments arranged by size:
2109 1697 959 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3697 160
Size: 872 2665 1606
Fragments arranged by size:
2665 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5143
AvaI C'yCGr_G
Cuts at: 1870 1943 2468 1870
Size: 73 525 4545
Fragments arranged by size:
4545 525 73
BamHI G'GATC_C
Cuts at: 2473 2840 2473
Size: 367 4776
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2333 2464 3919 618
Size: 325 217 627 546 131 1455 1842
Fragments arranged by size:
1842 1455 627 546 325 217 131
BanII G_rGCy'C
Cuts at: 1391 2462 1391
Size: 1071 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2441 928
Size: 971 542 3630
Fragments arranged by size:
3630 971 542
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3334 3368 1903
Size: 34 1397 34 3678
Fragments arranged by size:
3678 1397 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3035 4245 3035
Size: 1210 3933
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5143
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3754 136
Size: 107 122 71 631 605 2082 1525
Fragments arranged by size:
2082 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5138 5138
Size: 5143
BlpI GC'TnA_GC
Cuts at: 2560 2560
Size: 5143
Bme1580I G_kGCm'C
Cuts at: 1790 3204 4360 1790
Size: 1414 1156 2573
Fragments arranged by size:
2573 1414 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5143
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2874 3832 442
Size: 416 53 83 399 24 1457 958 1753
Fragments arranged by size:
1753 1457 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5143
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3803 1239
Size: 2564 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3268 4133 4431 3268
Size: 865 298 3980
Fragments arranged by size:
3980 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3806 882
Size: 617 2307 2219
Fragments arranged by size:
2307 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2373 2934 493
Size: 711 1169 561 2702
Fragments arranged by size:
2702 1169 711 561
BsaBI GATnn'nnATC
Cuts at: 5137 5137
Size: 5143
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2950 3332 3967 275
Size: 53 83 186 936 1417 382 635 1451
Fragments arranged by size:
1451 1417 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3576 3974 4248 4395 4806 3576
Size: 398 274 147 411 3913
Fragments arranged by size:
3913 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4918
Fragments arranged by size:
4918 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2398 1186
Size: 1212 3931
BseYI C'CCAG_C
Cuts at: 2188 4346 4842 2188
Size: 2158 496 2489
Fragments arranged by size:
2489 2158 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2011 1163
Size: 573 275 4295
Fragments arranged by size:
4295 573 275
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2593 3354 4382 1540
Size: 275 186 592 761 1028 2301
Fragments arranged by size:
2301 1028 761 592 275 186
BsiHKAI G_wGCw'C
Cuts at: 3204 3289 4360 3204
Size: 85 1071 3987
Fragments arranged by size:
3987 1071 85
BsmI GAATG_Cn'
Cuts at: 2654 2747 2654
Size: 93 5050
BsmBI CGTCTCn'nnnn_
Cuts at: 2233 2233
Size: 5143
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2462 3204 3289 4360 1391
Size: 399 672 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 672 399 85
BspEI T'CCGG_A
Cuts at: 4806 4806
Size: 5143
BspHI T'CATG_A
Cuts at: 2319 2382 3032 4762 2319
Size: 63 650 1730 2700
Fragments arranged by size:
2700 1730 650 63
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2436 2498 844
Size: 1592 62 3489
Fragments arranged by size:
3489 1592 62
BsrBI CCG'CTC
Cuts at: 1394 1823 3030 1394
Size: 429 1207 3507
Fragments arranged by size:
3507 1207 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3638 3812 65
Size: 1183 2390 174 1396
Fragments arranged by size:
2390 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3787 3974 1700
Size: 110 1977 187 2869
Fragments arranged by size:
2869 1977 187 110
BsrGI T'GTAC_A
Cuts at: 96 2349 96
Size: 2253 2890
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5143
BssSI C'ACGA_G
Cuts at: 3203 4215 3203
Size: 1012 4131
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5143
BstBI TT'CG_AA
Cuts at: 3947 3947
Size: 5143
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5143
BstYI r'GATC_y
Cuts at: 1972 2473 2840 3225 3242 4694 4780 4792 5138
Size: 501 367 385 17 1452 86 12 346
Cuts at: 5138 1972
Size: 1977
Fragments arranged by size:
1977 1452 501 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2421 2421
Size: 5143
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3652
Fragments arranged by size:
3652 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4404
Fragments arranged by size:
4404 683 56
BtsI GCAGTG_nn'
Cuts at: 2659 3453 3473 2659
Size: 794 20 4329
Fragments arranged by size:
4329 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5143
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5108
DraI TTT'AAA
Cuts at: 2447 2447
Size: 5143
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4150 809
Size: 507 2834 1802
Fragments arranged by size:
2834 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2590 3479 5108
Size: 54 1425 50 275 18 760 889 1629
Cuts at: 5108 8
Size: 43
Fragments arranged by size:
1629 1425 889 760 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2590 1537
Size: 275 778 4090
Fragments arranged by size:
4090 778 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3073 1353
Size: 1720 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3730 4104 4250 387
Size: 1064 138 2141 374 146 1280
Fragments arranged by size:
2141 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 3206 3803 4590 4858 1239
Size: 393 422 1152 597 787 268 1524
Fragments arranged by size:
1524 1152 787 597 422 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2304 2425 2304
Size: 121 5022
EcoRI G'AATT_C
Cuts at: 2452 2830 2452
Size: 378 4765
FspI TGC'GCA
Cuts at: 3649 3649
Size: 5143
HaeII r_GCGC'y
Cuts at: 1678 4290 1678
Size: 2612 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2276 2308 3784 3816 3858
Size: 32 135 32 704 32 1476 32 42
Cuts at: 3858 3890 1373
Size: 32 2626
Fragments arranged by size:
2626 1476 704 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2487 2733 669
Size: 988 281 549 246 3079
Fragments arranged by size:
3079 988 549 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5143
HpaI GTT'AAC
Cuts at: 2733 2733
Size: 5143
KpnI G_GTAC'C
Cuts at: 2468 2468
Size: 5143
MfeI C'AATT_G
Cuts at: 2256 2742 2256
Size: 486 4657
MluI A'CGCG_T
Cuts at: 5019 5019
Size: 5143
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4257 4441 1305
Size: 2952 184 2007
Fragments arranged by size:
2952 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3321
Fragments arranged by size:
3321 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3101 3460 3619 518
Size: 648 1935 359 159 2042
Fragments arranged by size:
2042 1935 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2056 2188 2559 3236 4384 4629 1606
Size: 450 132 371 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 450 371 245 132
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3652
Fragments arranged by size:
3652 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5143
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5143
NotI GC'GGCC_GC
Cuts at: 2590 2590
Size: 5143
NspI r_CATG'y
Cuts at: 1132 1229 2278 2501 4046 1132
Size: 97 1049 223 1545 2229
Fragments arranged by size:
2229 1545 1049 223 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5143
PciI A'CATG_T
Cuts at: 4042 4042
Size: 5143
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5143
PfoI T'CCnGG_A
Cuts at: 2391 2391
Size: 5143
PmeI GTTT'AAAC
Cuts at: 2447 2447
Size: 5143
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3212 3244 4725 4757 3212
Size: 32 1481 32 3598
Fragments arranged by size:
3598 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2425 2425
Size: 5143
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5143
PsiI TTA'TAA
Cuts at: 2713 2713
Size: 5143
PstI C_TGCA'G
Cuts at: 830 2312 2433 2495 830
Size: 1482 121 62 3478
Fragments arranged by size:
3478 1482 121 62
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5143
PvuII CAG'CTG
Cuts at: 2056 2056
Size: 5143
RsrII CG'GwC_CG
Cuts at: 2208 2208
Size: 5143
SalI G'TCGA_C
Cuts at: 1936 2485 1936
Size: 549 4594
SbfI CC_TGCA'GG
Cuts at: 2312 2495 2312
Size: 183 4960
ScaI AGT'ACT
Cuts at: 3391 5103 3391
Size: 1712 3431
SfcI C'TryA_G
Cuts at: 826 1045 2101 2308 2429 2491 3626 4307 4498
Size: 219 1056 207 121 62 1135 681 191
Cuts at: 4498 826
Size: 1471
Fragments arranged by size:
1471 1135 1056 681 219 207 191 121
62
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5143
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5143
SmaI CCC'GGG
Cuts at: 1872 2470 1872
Size: 598 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3247 4148 4410 820
Size: 197 926 1304 901 262 1553
Fragments arranged by size:
1553 1304 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2373 2934 493
Size: 1880 561 2702
Fragments arranged by size:
2702 1880 561
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5143
SphI G_CATG'C
Cuts at: 1132 2501 1132
Size: 1369 3774
SspI AAT'ATT
Cuts at: 5 52 3067 5
Size: 47 3015 2081
Fragments arranged by size:
3015 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5143
StyI C'CwwG_G
Cuts at: 513 1065 2004 2582 513
Size: 552 939 578 3074
Fragments arranged by size:
3074 939 578 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2150 3170 3187 3340 3525 3970 325
Size: 1255 570 1020 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1020 570 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2349 3389 5101
Size: 274 80 33 51 1443 372 1040 1712
Cuts at: 5101 96
Size: 138
Fragments arranged by size:
1712 1443 1040 372 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5103 5135 5103
Size: 32 5111
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5143
XbaI T'CTAG_A
Cuts at: 2479 2479
Size: 5143
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5143
XmaI C'CCGG_G
Cuts at: 1870 2468 1870
Size: 598 4545
XmnI GAAnn'nnTTC
Cuts at: 3272 3272
Size: 5143
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2951 276
Size: 53 83 186 936 1417 2468
Fragments arranged by size:
2468 1417 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII RsrII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI