Restriction map for
pFN21AE5135
(Circular) (Six-base) MAPSORT of: pFN21AE5135.seq Check: 2447 from: 1 to: 5029
pFN21AE5135 5029 bp
With 182 enzymes: SgfI *
January 30, 2014 17:13 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2839 278
Size: 53 83 186 936 1303 2468
Fragments arranged by size:
2468 1303 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2372 1937
Size: 435 4594
Acc65I G'GTAC_C
Cuts at: 2350 2350
Size: 5029
AclI AA'CG_TT
Cuts at: 1563 3156 3529 1563
Size: 1593 373 3063
Fragments arranged by size:
3063 1593 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3092 4476 4744 1632
Size: 1460 1384 268 1917
Fragments arranged by size:
1917 1460 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4832
AflIII A'CryG_T
Cuts at: 1203 2267 3928 4905 1203
Size: 1064 1661 977 1327
Fragments arranged by size:
1661 1327 1064 977
AgeI A'CCGG_T
Cuts at: 3860 3860
Size: 5029
AhdI GACnn_n'nnGTC
Cuts at: 2268 3758 2268
Size: 1490 3539
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5029
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5029
AlwNI CAG_nnn'CTG
Cuts at: 2772 4344 2772
Size: 1572 3457
ApaLI G'TGCA_C
Cuts at: 3086 4242 3086
Size: 1156 3873
ApoI r'AATT_y
Cuts at: 1493 2338 2564 2716 4825 1493
Size: 845 226 152 2109 1697
Fragments arranged by size:
2109 1697 845 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3583 160
Size: 872 2551 1606
Fragments arranged by size:
2551 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5029
AvaI C'yCGr_G
Cuts at: 1870 1943 2196 2354 1870
Size: 73 253 158 4545
Fragments arranged by size:
4545 253 158 73
BamHI G'GATC_C
Cuts at: 2097 2359 2726 2097
Size: 262 367 4400
Fragments arranged by size:
4400 367 262
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2350 3805 618
Size: 325 217 627 563 1455 1842
Fragments arranged by size:
1842 1455 627 563 325 217
BanII G_rGCy'C
Cuts at: 1391 2348 1391
Size: 957 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4058
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3220 3254 1903
Size: 34 1283 34 3678
Fragments arranged by size:
3678 1283 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2921 4131 2921
Size: 1210 3819
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5029
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2199 3640 136
Size: 107 122 71 631 605 527 1441 1525
Fragments arranged by size:
1525 1441 631 605 527 122 107 71
BglII A'GATC_T
Cuts at: 5024 5024
Size: 5029
BlpI GC'TnA_GC
Cuts at: 2446 2446
Size: 5029
Bme1580I G_kGCm'C
Cuts at: 1790 3090 4246 1790
Size: 1300 1156 2573
Fragments arranged by size:
2573 1300 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5029
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2760 3718 442
Size: 416 53 83 399 24 1343 958 1753
Fragments arranged by size:
1753 1343 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5029
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2155 2171 3689 1239
Size: 916 16 1518 2579
Fragments arranged by size:
2579 1518 916 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3154 4019 4317 3154
Size: 865 298 3866
Fragments arranged by size:
3866 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2255 3692 882
Size: 617 756 1437 2219
Fragments arranged by size:
2219 1437 756 617
BsaAI yAC'GTr
Cuts at: 493 1204 2820 493
Size: 711 1616 2702
Fragments arranged by size:
2702 1616 711
BsaBI GATnn'nnATC
Cuts at: 5023 5023
Size: 5029
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2836 3218 3853 275
Size: 53 83 186 936 1303 382 635 1451
Fragments arranged by size:
1451 1303 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2300 3462 3860 4134 4281 4692 2300
Size: 1162 398 274 147 411 2637
Fragments arranged by size:
2637 1162 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4804
Fragments arranged by size:
4804 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2012 2015 2018 1186
Size: 826 3 3 4197
Fragments arranged by size:
4197 826 3 3
BseYI C'CCAG_C
Cuts at: 4232 4728 4232
Size: 496 4533
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2203 2342 1163
Size: 573 467 139 3850
Fragments arranged by size:
3850 573 467 139
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2479 3240 4268 1540
Size: 275 186 478 761 1028 2301
Fragments arranged by size:
2301 1028 761 478 275 186
BsiHKAI G_wGCw'C
Cuts at: 2072 3090 3175 4246 2072
Size: 1018 85 1071 2855
Fragments arranged by size:
2855 1071 1018 85
BsmI GAATG_Cn'
Cuts at: 2540 2633 2540
Size: 93 4936
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 2348 3090 3175 4246 1391
Size: 399 282 276 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 282 276 85
BspEI T'CCGG_A
Cuts at: 4692 4692
Size: 5029
BspHI T'CATG_A
Cuts at: 2918 4648 2918
Size: 1730 3299
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2384 844
Size: 1540 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2916 1394
Size: 429 1093 3507
Fragments arranged by size:
3507 1093 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3524 3698 65
Size: 1183 2276 174 1396
Fragments arranged by size:
2276 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2111 2213 3673 3860 1700
Size: 110 301 102 1460 187 2869
Fragments arranged by size:
2869 1460 301 187 110 102
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5029
BssHII G'CGCG_C
Cuts at: 1925 2254 2306 1925
Size: 329 52 4648
Fragments arranged by size:
4648 329 52
BssSI C'ACGA_G
Cuts at: 2242 3089 4101 2242
Size: 847 1012 3170
Fragments arranged by size:
3170 1012 847
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5029
BstBI TT'CG_AA
Cuts at: 3833 3833
Size: 5029
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5029
BstYI r'GATC_y
Cuts at: 1972 2097 2359 2726 3111 3128 4580 4666 4678
Size: 125 262 367 385 17 1452 86 12
Cuts at: 4678 5024 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 262 125 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2073 513
Size: 552 1008 3469
Fragments arranged by size:
3469 1008 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4290
Fragments arranged by size:
4290 683 56
BtsI GCAGTG_nn'
Cuts at: 2272 2545 3339 3359 2272
Size: 273 794 20 3942
Fragments arranged by size:
3942 794 273 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5029
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4994
DraI TTT'AAA
Cuts at: 2333 2333
Size: 5029
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4036 809
Size: 507 2720 1802
Fragments arranged by size:
2720 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2476 3365 4994
Size: 54 1425 50 275 18 646 889 1629
Cuts at: 4994 8
Size: 43
Fragments arranged by size:
1629 1425 889 646 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2476 1537
Size: 275 664 4090
Fragments arranged by size:
4090 664 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2959 1353
Size: 1606 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2221 3616 3990 4136 387
Size: 1064 138 632 1395 374 146 1280
Fragments arranged by size:
1395 1280 1064 632 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2155 2171 3092 3689 4476 4744 1239
Size: 393 523 16 921 597 787 268 1524
Fragments arranged by size:
1524 921 787 597 523 393 268 16
EcoO109I rG'GnC_Cy
Cuts at: 2179 2179
Size: 5029
EcoRI G'AATT_C
Cuts at: 2338 2716 2338
Size: 378 4651
FspI TGC'GCA
Cuts at: 3535 3535
Size: 5029
HaeII r_GCGC'y
Cuts at: 1678 4176 1678
Size: 2498 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2254 2286 3670 3702 3744
Size: 32 135 32 682 32 1384 32 42
Cuts at: 3744 3776 1373
Size: 32 2626
Fragments arranged by size:
2626 1384 682 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2373 2619 669
Size: 988 281 435 246 3079
Fragments arranged by size:
3079 988 435 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5029
HpaI GTT'AAC
Cuts at: 2619 2619
Size: 5029
KpnI G_GTAC'C
Cuts at: 2354 2354
Size: 5029
MfeI C'AATT_G
Cuts at: 2628 2628
Size: 5029
MluI A'CGCG_T
Cuts at: 4905 4905
Size: 5029
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4143 4327 1305
Size: 2838 184 2007
Fragments arranged by size:
2838 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3207
Fragments arranged by size:
3207 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2987 3346 3505 518
Size: 648 1821 359 159 2042
Fragments arranged by size:
2042 1821 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2445 3122 4270 4515 1606
Size: 839 677 1148 245 2120
Fragments arranged by size:
2120 1148 839 677 245
NaeI GCC'GGC
Cuts at: 2113 2113
Size: 5029
NcoI C'CATG_G
Cuts at: 513 1065 2073 513
Size: 552 1008 3469
Fragments arranged by size:
3469 1008 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5029
NgoMIV G'CCGG_C
Cuts at: 2111 2111
Size: 5029
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5029
NotI GC'GGCC_GC
Cuts at: 2476 2476
Size: 5029
NspI r_CATG'y
Cuts at: 1132 1229 2271 2387 3932 1132
Size: 97 1042 116 1545 2229
Fragments arranged by size:
2229 1545 1042 116 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5029
PciI A'CATG_T
Cuts at: 2267 3928 2267
Size: 1661 3368
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5029
PfoI T'CCnGG_A
Cuts at: 2100 2100
Size: 5029
PmeI GTTT'AAAC
Cuts at: 2333 2333
Size: 5029
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3098 3130 4611 4643 3098
Size: 32 1481 32 3484
Fragments arranged by size:
3484 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2179 2179
Size: 5029
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5029
PsiI TTA'TAA
Cuts at: 2599 2599
Size: 5029
PstI C_TGCA'G
Cuts at: 830 2381 830
Size: 1551 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5029
SalI G'TCGA_C
Cuts at: 1936 2371 1936
Size: 435 4594
SbfI CC_TGCA'GG
Cuts at: 2381 2381
Size: 5029
ScaI AGT'ACT
Cuts at: 3277 4989 3277
Size: 1712 3317
SfcI C'TryA_G
Cuts at: 826 1045 2377 3512 4193 4384 826
Size: 219 1332 1135 681 191 1471
Fragments arranged by size:
1471 1332 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5029
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5029
SmaI CCC'GGG
Cuts at: 1872 2356 1872
Size: 484 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3133 4034 4296 820
Size: 197 926 1190 901 262 1553
Fragments arranged by size:
1553 1190 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2820 493
Size: 2327 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5029
SphI G_CATG'C
Cuts at: 1132 2387 1132
Size: 1255 3774
SspI AAT'ATT
Cuts at: 5 52 2124 2953 5
Size: 47 2072 829 2081
Fragments arranged by size:
2081 2072 829 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5029
StyI C'CwwG_G
Cuts at: 513 1065 2073 2468 513
Size: 552 1008 395 3074
Fragments arranged by size:
3074 1008 552 395
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3056 3073 3226 3411 3856 325
Size: 1255 1476 17 153 185 445 1498
Fragments arranged by size:
1498 1476 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3275 4987 96
Size: 274 80 33 51 1443 1298 1712 138
Fragments arranged by size:
1712 1443 1298 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4989 5021 4989
Size: 32 4997
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5029
XbaI T'CTAG_A
Cuts at: 2365 2365
Size: 5029
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2179 2179
Size: 5029
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5029
XmaI C'CCGG_G
Cuts at: 1870 2354 1870
Size: 484 4545
XmnI GAAnn'nnTTC
Cuts at: 3158 3158
Size: 5029
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2837 276
Size: 53 83 186 936 1303 2468
Fragments arranged by size:
2468 1303 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PmlI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI