Restriction map for
pFN21AE5127
(Circular) (Six-base) MAPSORT of: pFN21AE5127.seq Check: 5511 from: 1 to: 5059
pFN21AE5127 5059 bp
With 182 enzymes: SgfI *
January 30, 2014 17:12 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2869 278
Size: 53 83 186 936 1333 2468
Fragments arranged by size:
2468 1333 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2402 1937
Size: 465 4594
Acc65I G'GTAC_C
Cuts at: 2380 2380
Size: 5059
AclI AA'CG_TT
Cuts at: 1563 3186 3559 1563
Size: 1623 373 3063
Fragments arranged by size:
3063 1623 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3122 4506 4774 1632
Size: 1490 1384 268 1917
Fragments arranged by size:
1917 1490 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4862
AflIII A'CryG_T
Cuts at: 1203 3958 4935 1203
Size: 2755 977 1327
Fragments arranged by size:
2755 1327 977
AgeI A'CCGG_T
Cuts at: 3890 3890
Size: 5059
AhdI GACnn_n'nnGTC
Cuts at: 3788 3788
Size: 5059
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5059
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5059
AlwNI CAG_nnn'CTG
Cuts at: 2802 4374 2802
Size: 1572 3487
ApaLI G'TGCA_C
Cuts at: 3116 4272 3116
Size: 1156 3903
ApoI r'AATT_y
Cuts at: 1493 2029 2368 2594 2746 4855 1493
Size: 536 339 226 152 2109 1697
Fragments arranged by size:
2109 1697 536 339 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3613 160
Size: 872 2581 1606
Fragments arranged by size:
2581 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5059
AvaI C'yCGr_G
Cuts at: 1870 1943 2384 1870
Size: 73 441 4545
Fragments arranged by size:
4545 441 73
BamHI G'GATC_C
Cuts at: 2389 2756 2389
Size: 367 4692
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2210 2380 3835 618
Size: 325 217 627 423 170 1455 1842
Fragments arranged by size:
1842 1455 627 423 325 217 170
BanII G_rGCy'C
Cuts at: 1391 2172 2378 1391
Size: 781 206 4072
Fragments arranged by size:
4072 781 206
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4088
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3250 3284 1903
Size: 34 50 34 1229 34 3678
Fragments arranged by size:
3678 1229 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2283 2951 4161 2283
Size: 668 1210 3181
Fragments arranged by size:
3181 1210 668
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5059
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2056 3670 136
Size: 107 122 71 631 605 384 1614 1525
Fragments arranged by size:
1614 1525 631 605 384 122 107 71
BglII A'GATC_T
Cuts at: 5054 5054
Size: 5059
BlpI GC'TnA_GC
Cuts at: 2153 2476 2153
Size: 323 4736
Bme1580I G_kGCm'C
Cuts at: 1790 2052 2213 3120 4276 1790
Size: 262 161 907 1156 2573
Fragments arranged by size:
2573 1156 907 262 161
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5059
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2790 3748 442
Size: 416 53 83 399 24 1373 958 1753
Fragments arranged by size:
1753 1373 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2273 1056
Size: 1217 3842
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2036 2081 3719 1239
Size: 797 45 1638 2579
Fragments arranged by size:
2579 1638 797 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3184 4049 4347 3184
Size: 865 298 3896
Fragments arranged by size:
3896 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3722 882
Size: 617 2223 2219
Fragments arranged by size:
2223 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2850 493
Size: 711 1646 2702
Fragments arranged by size:
2702 1646 711
BsaBI GATnn'nnATC
Cuts at: 2294 5053 2294
Size: 2759 2300
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2866 3248 3883 275
Size: 53 83 186 936 1333 382 635 1451
Fragments arranged by size:
1451 1333 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2286 3492 3890 4164 4311 4722 2286
Size: 1206 398 274 147 411 2623
Fragments arranged by size:
2623 1206 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4834
Fragments arranged by size:
4834 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2182 1186
Size: 996 4063
BseYI C'CCAG_C
Cuts at: 4262 4758 4262
Size: 496 4563
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4486
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2135 2509 3270 4298 1540
Size: 275 186 134 374 761 1028 2301
Fragments arranged by size:
2301 1028 761 374 275 186 134
BsiHKAI G_wGCw'C
Cuts at: 3120 3205 4276 3120
Size: 85 1071 3903
Fragments arranged by size:
3903 1071 85
BsmI GAATG_Cn'
Cuts at: 2570 2663 2570
Size: 93 4966
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2172 2213 2378 3120 3205 4276
Size: 399 262 120 41 165 742 85 1071
Cuts at: 4276 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 262 165 120 85
41
BspEI T'CCGG_A
Cuts at: 4722 4722
Size: 5059
BspHI T'CATG_A
Cuts at: 2346 2948 4678 2346
Size: 602 1730 2727
Fragments arranged by size:
2727 1730 602
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2113 2414 844
Size: 1269 301 3489
Fragments arranged by size:
3489 1269 301
BsrBI CCG'CTC
Cuts at: 1394 1823 2157 2946 1394
Size: 429 334 789 3507
Fragments arranged by size:
3507 789 429 334
BsrDI GCAATG_nn'
Cuts at: 65 1248 3554 3728 65
Size: 1183 2306 174 1396
Fragments arranged by size:
2306 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3703 3890 1700
Size: 110 1893 187 2869
Fragments arranged by size:
2869 1893 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5059
BssHII G'CGCG_C
Cuts at: 1925 2175 1925
Size: 250 4809
BssSI C'ACGA_G
Cuts at: 3119 4131 3119
Size: 1012 4047
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5059
BstBI TT'CG_AA
Cuts at: 3863 3863
Size: 5059
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5059
BstYI r'GATC_y
Cuts at: 1972 2389 2756 3141 3158 4610 4696 4708 5054
Size: 417 367 385 17 1452 86 12 346
Cuts at: 5054 1972
Size: 1977
Fragments arranged by size:
1977 1452 417 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2052 2145 513
Size: 552 987 93 3427
Fragments arranged by size:
3427 987 552 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4320
Fragments arranged by size:
4320 683 56
BtsI GCAGTG_nn'
Cuts at: 2575 3369 3389 2575
Size: 794 20 4245
Fragments arranged by size:
4245 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5059
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5024
DraI TTT'AAA
Cuts at: 2363 2363
Size: 5059
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4066 809
Size: 507 2750 1802
Fragments arranged by size:
2750 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2132 2142 2506
Size: 54 1425 50 275 18 302 10 364
Cuts at: 2506 3395 5024 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 364 302 275 54 50
43 18 10
EagI C'GGCC_G
Cuts at: 1537 1812 2132 2506 1537
Size: 275 320 374 4090
Fragments arranged by size:
4090 374 320 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2989 1353
Size: 1636 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3646 4020 4166 387
Size: 1064 138 2057 374 146 1280
Fragments arranged by size:
2057 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2036 2081 3122 3719 4506 4774 1239
Size: 393 404 45 1041 597 787 268 1524
Fragments arranged by size:
1524 1041 787 597 404 393 268 45
EcoNI CCTnn'n_nnAGG
Cuts at: 2279 2279
Size: 5059
EcoRI G'AATT_C
Cuts at: 2368 2746 2368
Size: 378 4681
FspI TGC'GCA
Cuts at: 3565 3565
Size: 5059
HaeII r_GCGC'y
Cuts at: 1678 2153 2241 4206 1678
Size: 475 88 1965 2531
Fragments arranged by size:
2531 1965 475 88
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3700 3732 3774 3806 1373
Size: 32 135 32 2128 32 42 32 2626
Fragments arranged by size:
2626 2128 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2403 2649 669
Size: 988 281 465 246 3079
Fragments arranged by size:
3079 988 465 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5059
HpaI GTT'AAC
Cuts at: 2649 2649
Size: 5059
KpnI G_GTAC'C
Cuts at: 2384 2384
Size: 5059
MfeI C'AATT_G
Cuts at: 2658 2658
Size: 5059
MluI A'CGCG_T
Cuts at: 4935 4935
Size: 5059
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4173 4357 1305
Size: 2868 184 2007
Fragments arranged by size:
2868 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2144 10
Size: 54 1425 343 312 2925
Fragments arranged by size:
2925 1425 343 312 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3017 3376 3535 518
Size: 648 1851 359 159 2042
Fragments arranged by size:
2042 1851 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2054 2084 2182 2475 3152 4300 4545 1606
Size: 448 30 98 293 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 448 293 245 98 30
NcoI C'CATG_G
Cuts at: 513 1065 2145 513
Size: 552 1080 3427
Fragments arranged by size:
3427 1080 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5059
NheI G'CTAG_C
Cuts at: 1052 2269 1052
Size: 1217 3842
NotI GC'GGCC_GC
Cuts at: 2506 2506
Size: 5059
NspI r_CATG'y
Cuts at: 1132 1229 2417 3962 1132
Size: 97 1188 1545 2229
Fragments arranged by size:
2229 1545 1188 97
PasI CC'CwG_GG
Cuts at: 1348 2275 1348
Size: 927 4132
PciI A'CATG_T
Cuts at: 3958 3958
Size: 5059
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5059
PfoI T'CCnGG_A
Cuts at: 2013 2013
Size: 5059
PmeI GTTT'AAAC
Cuts at: 2363 2363
Size: 5059
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3128 3160 4641 4673 3128
Size: 32 1481 32 3514
Fragments arranged by size:
3514 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5059
PsiI TTA'TAA
Cuts at: 2629 2629
Size: 5059
PstI C_TGCA'G
Cuts at: 830 2411 830
Size: 1581 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5059
PvuII CAG'CTG
Cuts at: 2182 2182
Size: 5059
RsrII CG'GwC_CG
Cuts at: 2110 2110
Size: 5059
SacII CC_GC'GG
Cuts at: 2055 2055
Size: 5059
SalI G'TCGA_C
Cuts at: 1936 2401 1936
Size: 465 4594
SbfI CC_TGCA'GG
Cuts at: 2411 2411
Size: 5059
ScaI AGT'ACT
Cuts at: 3307 5019 3307
Size: 1712 3347
SfcI C'TryA_G
Cuts at: 826 1045 2407 3542 4223 4414 826
Size: 219 1362 1135 681 191 1471
Fragments arranged by size:
1471 1362 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5059
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5059
SmaI CCC'GGG
Cuts at: 1872 2386 1872
Size: 514 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3163 4064 4326 820
Size: 197 926 1220 901 262 1553
Fragments arranged by size:
1553 1220 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2850 493
Size: 2357 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5059
SphI G_CATG'C
Cuts at: 1132 2417 1132
Size: 1285 3774
SspI AAT'ATT
Cuts at: 5 52 2983 5
Size: 47 2931 2081
Fragments arranged by size:
2931 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5059
StyI C'CwwG_G
Cuts at: 513 1065 2145 2262 2310 2498 513
Size: 552 1080 117 48 188 3074
Fragments arranged by size:
3074 1080 552 188 117 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3086 3103 3256 3441 3886 325
Size: 1255 1506 17 153 185 445 1498
Fragments arranged by size:
1506 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3305 5017 96
Size: 274 80 33 51 1443 1328 1712 138
Fragments arranged by size:
1712 1443 1328 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5019 5051 5019
Size: 32 5027
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5059
XbaI T'CTAG_A
Cuts at: 2395 2395
Size: 5059
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2203 2011
Size: 192 4867
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5059
XmaI C'CCGG_G
Cuts at: 1870 2384 1870
Size: 514 4545
XmnI GAAnn'nnTTC
Cuts at: 3188 3188
Size: 5059
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2867 276
Size: 53 83 186 936 1333 2468
Fragments arranged by size:
2468 1333 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII RsrII SacII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PsrI SacI SanDI SapI SexAI
SfiI SfoI SrfI SwaI