Restriction map for
pFN21AE5126
(Circular) (Six-base) MAPSORT of: pFN21AE5126.seq Check: 1597 from: 1 to: 5128
pFN21AE5126 5128 bp
With 182 enzymes: SgfI *
December 8, 2014 13:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2000 2000
Size: 5128
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2938 278
Size: 53 83 186 936 1402 2468
Fragments arranged by size:
2468 1402 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2471 1937
Size: 534 4594
Acc65I G'GTAC_C
Cuts at: 2449 2449
Size: 5128
AclI AA'CG_TT
Cuts at: 1563 3255 3628 1563
Size: 1692 373 3063
Fragments arranged by size:
3063 1692 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2120 3191 4575 4843 1632
Size: 488 1071 1384 268 1917
Fragments arranged by size:
1917 1384 1071 488 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4931
AflIII A'CryG_T
Cuts at: 1203 4027 5004 1203
Size: 2824 977 1327
Fragments arranged by size:
2824 1327 977
AgeI A'CCGG_T
Cuts at: 3959 3959
Size: 5128
AhdI GACnn_n'nnGTC
Cuts at: 3857 3857
Size: 5128
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5128
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5128
AlwNI CAG_nnn'CTG
Cuts at: 2871 4443 2871
Size: 1572 3556
ApaLI G'TGCA_C
Cuts at: 3185 4341 3185
Size: 1156 3972
ApoI r'AATT_y
Cuts at: 1493 2043 2071 2194 2437 2663 2815 4924 1493
Size: 550 28 123 243 226 152 2109 1697
Fragments arranged by size:
2109 1697 550 243 226 152 123 28
AseI AT'TA_AT
Cuts at: 160 1032 2022 3682 160
Size: 872 990 1660 1606
Fragments arranged by size:
1660 1606 990 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5128
AvaI C'yCGr_G
Cuts at: 1870 1943 2453 1870
Size: 73 510 4545
Fragments arranged by size:
4545 510 73
BamHI G'GATC_C
Cuts at: 2146 2458 2825 2146
Size: 312 367 4449
Fragments arranged by size:
4449 367 312
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2449 3904 618
Size: 325 217 627 662 1455 1842
Fragments arranged by size:
1842 1455 662 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2447 1391
Size: 1056 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2034 2108 928
Size: 971 135 74 3948
Fragments arranged by size:
3948 971 135 74
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3319 3353 1903
Size: 34 1382 34 3678
Fragments arranged by size:
3678 1382 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3020 4230 3020
Size: 1210 3918
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5128
BfrBI ATG'CAT
Cuts at: 2393 2393
Size: 5128
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3739 136
Size: 107 122 71 631 605 2067 1525
Fragments arranged by size:
2067 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5123 5123
Size: 5128
BlpI GC'TnA_GC
Cuts at: 2545 2545
Size: 5128
Bme1580I G_kGCm'C
Cuts at: 1790 3189 4345 1790
Size: 1399 1156 2573
Fragments arranged by size:
2573 1399 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5128
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2859 3817 442
Size: 416 53 83 399 24 1442 958 1753
Fragments arranged by size:
1753 1442 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5128
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3788 1239
Size: 2549 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3253 4118 4416 3253
Size: 865 298 3965
Fragments arranged by size:
3965 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3791 882
Size: 617 2292 2219
Fragments arranged by size:
2292 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2919 493
Size: 711 1715 2702
Fragments arranged by size:
2702 1715 711
BsaBI GATnn'nnATC
Cuts at: 5122 5122
Size: 5128
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2935 3317 3952 275
Size: 53 83 186 936 1402 382 635 1451
Fragments arranged by size:
1451 1402 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3561 3959 4233 4380 4791 3561
Size: 398 274 147 411 3898
Fragments arranged by size:
3898 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4903
Fragments arranged by size:
4903 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5128
BseYI C'CCAG_C
Cuts at: 4331 4827 4331
Size: 496 4632
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2361 1163
Size: 573 625 3930
Fragments arranged by size:
3930 625 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2578 3339 4367 1540
Size: 275 186 577 761 1028 2301
Fragments arranged by size:
2301 1028 761 577 275 186
BsiHKAI G_wGCw'C
Cuts at: 3189 3274 4345 3189
Size: 85 1071 3972
Fragments arranged by size:
3972 1071 85
BsmI GAATG_Cn'
Cuts at: 2639 2732 2639
Size: 93 5035
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2447 3189 3274 4345 1391
Size: 399 657 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 657 399 85
BspEI T'CCGG_A
Cuts at: 4791 4791
Size: 5128
BspHI T'CATG_A
Cuts at: 3017 4747 3017
Size: 1730 3398
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2000 2483 844
Size: 1156 483 3489
Fragments arranged by size:
3489 1156 483
BsrBI CCG'CTC
Cuts at: 1394 1823 3015 1394
Size: 429 1192 3507
Fragments arranged by size:
3507 1192 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3623 3797 65
Size: 1183 2375 174 1396
Fragments arranged by size:
2375 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3772 3959 1700
Size: 110 1962 187 2869
Fragments arranged by size:
2869 1962 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5128
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5128
BssSI C'ACGA_G
Cuts at: 2048 3188 4200 2048
Size: 1140 1012 2976
Fragments arranged by size:
2976 1140 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5128
BstBI TT'CG_AA
Cuts at: 3932 3932
Size: 5128
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5128
BstYI r'GATC_y
Cuts at: 1972 2146 2458 2825 3210 3227 4679 4765 4777
Size: 174 312 367 385 17 1452 86 12
Cuts at: 4777 5123 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 312 174 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2238 2238
Size: 5128
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3637
Fragments arranged by size:
3637 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4389
Fragments arranged by size:
4389 683 56
BtsI GCAGTG_nn'
Cuts at: 2644 3438 3458 2644
Size: 794 20 4314
Fragments arranged by size:
4314 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5128
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5093
DraI TTT'AAA
Cuts at: 2432 2432
Size: 5128
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2110 4135 809
Size: 507 794 2025 1802
Fragments arranged by size:
2025 1802 794 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2575 3464 5093
Size: 54 1425 50 275 18 745 889 1629
Cuts at: 5093 8
Size: 43
Fragments arranged by size:
1629 1425 889 745 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2575 1537
Size: 275 763 4090
Fragments arranged by size:
4090 763 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3058 1353
Size: 1705 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3715 4089 4235 387
Size: 1064 138 2126 374 146 1280
Fragments arranged by size:
2126 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2120 3191 3788 4575 4843 1239
Size: 393 488 1071 597 787 268 1524
Fragments arranged by size:
1524 1071 787 597 488 393 268
EcoRI G'AATT_C
Cuts at: 2071 2437 2815 2071
Size: 366 378 4384
Fragments arranged by size:
4384 378 366
FspI TGC'GCA
Cuts at: 3634 3634
Size: 5128
HaeII r_GCGC'y
Cuts at: 1678 4275 1678
Size: 2597 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2042 2074 3769 3801 3843
Size: 32 135 32 470 32 1695 32 42
Cuts at: 3843 3875 1373
Size: 32 2626
Fragments arranged by size:
2626 1695 470 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2472 2718 669
Size: 988 281 534 246 3079
Fragments arranged by size:
3079 988 534 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5128
HpaI GTT'AAC
Cuts at: 2718 2718
Size: 5128
KpnI G_GTAC'C
Cuts at: 2453 2453
Size: 5128
MfeI C'AATT_G
Cuts at: 2727 2727
Size: 5128
MluI A'CGCG_T
Cuts at: 5004 5004
Size: 5128
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2124 4242 4426 1305
Size: 819 2118 184 2007
Fragments arranged by size:
2118 2007 819 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3306
Fragments arranged by size:
3306 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3086 3445 3604 518
Size: 648 1920 359 159 2042
Fragments arranged by size:
2042 1920 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2544 3221 4369 4614 1606
Size: 938 677 1148 245 2120
Fragments arranged by size:
2120 1148 938 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3637
Fragments arranged by size:
3637 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5128
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5128
NotI GC'GGCC_GC
Cuts at: 2575 2575
Size: 5128
NsiI A_TGCA'T
Cuts at: 2395 2395
Size: 5128
NspI r_CATG'y
Cuts at: 1132 1229 2486 4031 1132
Size: 97 1257 1545 2229
Fragments arranged by size:
2229 1545 1257 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5128
PciI A'CATG_T
Cuts at: 4027 4027
Size: 5128
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5128
PmeI GTTT'AAAC
Cuts at: 2432 2432
Size: 5128
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3197 3229 4710 4742 3197
Size: 32 1481 32 3583
Fragments arranged by size:
3583 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5128
PsiI TTA'TAA
Cuts at: 2698 2698
Size: 5128
PstI C_TGCA'G
Cuts at: 830 2480 830
Size: 1650 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5128
SalI G'TCGA_C
Cuts at: 1936 2470 1936
Size: 534 4594
SbfI CC_TGCA'GG
Cuts at: 2480 2480
Size: 5128
ScaI AGT'ACT
Cuts at: 3376 5088 3376
Size: 1712 3416
SfcI C'TryA_G
Cuts at: 826 1045 2476 3611 4292 4483 826
Size: 219 1431 1135 681 191 1471
Fragments arranged by size:
1471 1431 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5128
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5128
SmaI CCC'GGG
Cuts at: 1872 2455 1872
Size: 583 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3232 4133 4395 820
Size: 197 926 1289 901 262 1553
Fragments arranged by size:
1553 1289 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2919 493
Size: 2426 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5128
SphI G_CATG'C
Cuts at: 1132 2486 1132
Size: 1354 3774
SspI AAT'ATT
Cuts at: 5 52 3052 5
Size: 47 3000 2081
Fragments arranged by size:
3000 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5128
StyI C'CwwG_G
Cuts at: 513 1065 2004 2224 2567 513
Size: 552 939 220 343 3074
Fragments arranged by size:
3074 939 552 343 220
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3155 3172 3325 3510 3955 325
Size: 1255 1575 17 153 185 445 1498
Fragments arranged by size:
1575 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3374 5086 96
Size: 274 80 33 51 1443 1397 1712 138
Fragments arranged by size:
1712 1443 1397 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5088 5120 5088
Size: 32 5096
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5128
XbaI T'CTAG_A
Cuts at: 2120 2464 2120
Size: 344 4784
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2083 2083
Size: 5128
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5128
XmaI C'CCGG_G
Cuts at: 1870 2453 1870
Size: 583 4545
XmnI GAAnn'nnTTC
Cuts at: 3257 3257
Size: 5128
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2936 276
Size: 53 83 186 936 1402 2468
Fragments arranged by size:
2468 1402 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI