Restriction map for
pFN21AE5115
(Circular) (Six-base) MAPSORT of: pFN21AE5115.seq Check: 4781 from: 1 to: 5023
pFN21AE5115 5023 bp
With 182 enzymes: SgfI *
January 30, 2014 17:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2078 2078
Size: 5023
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2833 278
Size: 53 83 186 936 1297 2468
Fragments arranged by size:
2468 1297 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2366 1937
Size: 429 4594
Acc65I G'GTAC_C
Cuts at: 2344 2344
Size: 5023
AclI AA'CG_TT
Cuts at: 1563 3150 3523 1563
Size: 1587 373 3063
Fragments arranged by size:
3063 1587 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3086 4470 4738 1632
Size: 1454 1384 268 1917
Fragments arranged by size:
1917 1454 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4826
AflIII A'CryG_T
Cuts at: 1203 3922 4899 1203
Size: 2719 977 1327
Fragments arranged by size:
2719 1327 977
AgeI A'CCGG_T
Cuts at: 3854 3854
Size: 5023
AhdI GACnn_n'nnGTC
Cuts at: 3752 3752
Size: 5023
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5023
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5023
AlwNI CAG_nnn'CTG
Cuts at: 2766 4338 2766
Size: 1572 3451
ApaI G_GGCC'C
Cuts at: 2092 2092
Size: 5023
ApaLI G'TGCA_C
Cuts at: 3080 4236 3080
Size: 1156 3867
ApoI r'AATT_y
Cuts at: 1493 2135 2332 2558 2710 4819 1493
Size: 642 197 226 152 2109 1697
Fragments arranged by size:
2109 1697 642 226 197 152
AseI AT'TA_AT
Cuts at: 160 1032 3577 160
Size: 872 2545 1606
Fragments arranged by size:
2545 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5023
AvaI C'yCGr_G
Cuts at: 1870 1943 2217 2348 1870
Size: 73 274 131 4545
Fragments arranged by size:
4545 274 131 73
BamHI G'GATC_C
Cuts at: 2353 2720 2353
Size: 367 4656
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2212 2344 3799 618
Size: 325 217 627 425 132 1455 1842
Fragments arranged by size:
1842 1455 627 425 325 217 132
BanII G_rGCy'C
Cuts at: 1391 2092 2317 2342 1391
Size: 701 225 25 4072
Fragments arranged by size:
4072 701 225 25
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4052
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3214 3248 1903
Size: 34 1277 34 3678
Fragments arranged by size:
3678 1277 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2915 4125 2915
Size: 1210 3813
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5023
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3634 136
Size: 107 122 71 631 605 1962 1525
Fragments arranged by size:
1962 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5018 5018
Size: 5023
BlpI GC'TnA_GC
Cuts at: 2440 2440
Size: 5023
Bme1580I G_kGCm'C
Cuts at: 1790 2092 3084 4240 1790
Size: 302 992 1156 2573
Fragments arranged by size:
2573 1156 992 302
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5023
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2754 3712 442
Size: 416 53 83 399 24 1337 958 1753
Fragments arranged by size:
1753 1337 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5023
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3683 1239
Size: 2444 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1995 3148 4013 4311 1995
Size: 1153 865 298 2707
Fragments arranged by size:
2707 1153 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3686 882
Size: 617 2187 2219
Fragments arranged by size:
2219 2187 617
BsaAI yAC'GTr
Cuts at: 493 1204 2814 493
Size: 711 1610 2702
Fragments arranged by size:
2702 1610 711
BsaBI GATnn'nnATC
Cuts at: 5017 5017
Size: 5023
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2830 3212 3847 275
Size: 53 83 186 936 1297 382 635 1451
Fragments arranged by size:
1451 1297 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3456 3854 4128 4275 4686 3456
Size: 398 274 147 411 3793
Fragments arranged by size:
3793 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4798
Fragments arranged by size:
4798 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5023
BseYI C'CCAG_C
Cuts at: 4226 4722 4226
Size: 496 4527
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4450
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2473 3234 4262 1540
Size: 275 186 472 761 1028 2301
Fragments arranged by size:
2301 1028 761 472 275 186
BsiHKAI G_wGCw'C
Cuts at: 2317 3084 3169 4240 2317
Size: 767 85 1071 3100
Fragments arranged by size:
3100 1071 767 85
BsmI GAATG_Cn'
Cuts at: 2534 2627 2534
Size: 93 4930
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2092 2317 2342 3084 3169 4240 1391
Size: 399 302 225 25 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 302 225 85 25
BspEI T'CCGG_A
Cuts at: 4686 4686
Size: 5023
BspHI T'CATG_A
Cuts at: 2912 4642 2912
Size: 1730 3293
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2078 2311 2378 844
Size: 1234 233 67 3489
Fragments arranged by size:
3489 1234 233 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2910 1394
Size: 429 1087 3507
Fragments arranged by size:
3507 1087 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3518 3692 65
Size: 1183 2270 174 1396
Fragments arranged by size:
2270 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3667 3854 1700
Size: 110 1857 187 2869
Fragments arranged by size:
2869 1857 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5023
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5023
BssSI C'ACGA_G
Cuts at: 3083 4095 3083
Size: 1012 4011
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5023
BstBI TT'CG_AA
Cuts at: 3827 3827
Size: 5023
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5023
BstYI r'GATC_y
Cuts at: 1972 2097 2353 2720 3105 3122 4574 4660 4672
Size: 125 256 367 385 17 1452 86 12
Cuts at: 4672 5018 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 256 125 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3532
Fragments arranged by size:
3532 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4284
Fragments arranged by size:
4284 683 56
BtsI GCAGTG_nn'
Cuts at: 2260 2539 3333 3353 2260
Size: 279 794 20 3930
Fragments arranged by size:
3930 794 279 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5023
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4988
DraI TTT'AAA
Cuts at: 2327 2327
Size: 5023
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4030 809
Size: 507 2714 1802
Fragments arranged by size:
2714 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2470 3359 4988
Size: 54 1425 50 275 18 640 889 1629
Cuts at: 4988 8
Size: 43
Fragments arranged by size:
1629 1425 889 640 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2470 1537
Size: 275 658 4090
Fragments arranged by size:
4090 658 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2953 1353
Size: 1600 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2262 3610 3984 4130 387
Size: 1064 138 673 1348 374 146 1280
Fragments arranged by size:
1348 1280 1064 673 374 146 138
EcoICRI GAG'CTC
Cuts at: 2315 2315
Size: 5023
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3086 3683 4470 4738 1239
Size: 393 1454 597 787 268 1524
Fragments arranged by size:
1524 1454 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2088 2088
Size: 5023
EcoRI G'AATT_C
Cuts at: 2332 2710 2332
Size: 378 4645
EcoRV GAT'ATC
Cuts at: 2146 2146
Size: 5023
FspI TGC'GCA
Cuts at: 3529 3529
Size: 5023
HaeII r_GCGC'y
Cuts at: 1678 4170 1678
Size: 2492 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2089 2121 2249 2281 2299
Size: 32 135 32 517 32 128 32 18
Cuts at: 2299 2331 3664 3696 3738 3770 1373
Size: 32 1333 32 42 32 2626
Fragments arranged by size:
2626 1333 517 135 128 42 32 32
32 32 32 32 32 18
HincII GTy'rAC
Cuts at: 669 1657 1938 2367 2613 669
Size: 988 281 429 246 3079
Fragments arranged by size:
3079 988 429 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5023
HpaI GTT'AAC
Cuts at: 2613 2613
Size: 5023
KpnI G_GTAC'C
Cuts at: 2348 2348
Size: 5023
MfeI C'AATT_G
Cuts at: 2108 2622 2108
Size: 514 4509
MluI A'CGCG_T
Cuts at: 4899 4899
Size: 5023
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4137 4321 1305
Size: 2832 184 2007
Fragments arranged by size:
2832 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3201
Fragments arranged by size:
3201 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2981 3340 3499 518
Size: 648 1815 359 159 2042
Fragments arranged by size:
2042 1815 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2439 3116 4264 4509 1606
Size: 833 677 1148 245 2120
Fragments arranged by size:
2120 1148 833 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3532
Fragments arranged by size:
3532 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5023
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5023
NotI GC'GGCC_GC
Cuts at: 2470 2470
Size: 5023
NspI r_CATG'y
Cuts at: 1132 1229 2381 3926 1132
Size: 97 1152 1545 2229
Fragments arranged by size:
2229 1545 1152 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5023
PciI A'CATG_T
Cuts at: 3922 3922
Size: 5023
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5023
PmeI GTTT'AAAC
Cuts at: 2327 2327
Size: 5023
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2200 2232 3092 3124 4605 4637 2200
Size: 32 860 32 1481 32 2586
Fragments arranged by size:
2586 1481 860 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5023
PsiI TTA'TAA
Cuts at: 2593 2593
Size: 5023
PspOMI G'GGCC_C
Cuts at: 2088 2088
Size: 5023
PspXI vC'TCGA_Gb
Cuts at: 2217 2217
Size: 5023
PstI C_TGCA'G
Cuts at: 830 2084 2322 2375 830
Size: 1254 238 53 3478
Fragments arranged by size:
3478 1254 238 53
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5023
SacI G_AGCT'C
Cuts at: 2317 2317
Size: 5023
SalI G'TCGA_C
Cuts at: 1936 2365 1936
Size: 429 4594
SbfI CC_TGCA'GG
Cuts at: 2375 2375
Size: 5023
ScaI AGT'ACT
Cuts at: 3271 4983 3271
Size: 1712 3311
SfcI C'TryA_G
Cuts at: 826 1045 2080 2318 2371 3506 4187 4378 826
Size: 219 1035 238 53 1135 681 191 1471
Fragments arranged by size:
1471 1135 1035 681 238 219 191 53
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5023
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5023
SmaI CCC'GGG
Cuts at: 1872 2350 1872
Size: 478 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2010 2217 3127 4028 4290 820
Size: 197 926 67 207 910 901 262 1553
Fragments arranged by size:
1553 926 910 901 262 207 197 67
SnaBI TAC'GTA
Cuts at: 493 2814 493
Size: 2321 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5023
SphI G_CATG'C
Cuts at: 1132 2381 1132
Size: 1249 3774
SspI AAT'ATT
Cuts at: 5 52 2947 5
Size: 47 2895 2081
Fragments arranged by size:
2895 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5023
StyI C'CwwG_G
Cuts at: 513 1065 2004 2462 513
Size: 552 939 458 3074
Fragments arranged by size:
3074 939 552 458
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3050 3067 3220 3405 3850 325
Size: 1255 1470 17 153 185 445 1498
Fragments arranged by size:
1498 1470 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3269 4981 96
Size: 274 80 33 51 1443 1292 1712 138
Fragments arranged by size:
1712 1443 1292 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4983 5015 4983
Size: 32 4991
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5023
XbaI T'CTAG_A
Cuts at: 2359 2359
Size: 5023
XhoI C'TCGA_G
Cuts at: 1943 2217 1943
Size: 274 4749
XmaI C'CCGG_G
Cuts at: 1870 2348 1870
Size: 478 4545
XmnI GAAnn'nnTTC
Cuts at: 3152 3152
Size: 5023
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2831 276
Size: 53 83 186 936 1297 2468
Fragments arranged by size:
2468 1297 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI
PstI PvuI SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PsrI PvuII
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI