Restriction map for
pFN21AE5108
(Circular) (Six-base) MAPSORT of: pFN21AE5108.seq Check: 2202 from: 1 to: 5119
pFN21AE5108 5119 bp
With 182 enzymes: SgfI *
January 30, 2014 17:12 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2929 278
Size: 53 83 186 936 1393 2468
Fragments arranged by size:
2468 1393 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2462 1937
Size: 525 4594
Acc65I G'GTAC_C
Cuts at: 2440 2440
Size: 5119
AclI AA'CG_TT
Cuts at: 1563 3246 3619 1563
Size: 1683 373 3063
Fragments arranged by size:
3063 1683 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3182 4566 4834 1632
Size: 1550 1384 268 1917
Fragments arranged by size:
1917 1550 1384 268
AfeI AGC'GCT
Cuts at: 2407 2407
Size: 5119
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4922
AflIII A'CryG_T
Cuts at: 1203 4018 4995 1203
Size: 2815 977 1327
Fragments arranged by size:
2815 1327 977
AgeI A'CCGG_T
Cuts at: 3950 3950
Size: 5119
AhdI GACnn_n'nnGTC
Cuts at: 3848 3848
Size: 5119
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5119
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5119
AlwNI CAG_nnn'CTG
Cuts at: 2032 2155 2862 4434 2032
Size: 123 707 1572 2717
Fragments arranged by size:
2717 1572 707 123
ApaLI G'TGCA_C
Cuts at: 2161 3176 4332 2161
Size: 1015 1156 2948
Fragments arranged by size:
2948 1156 1015
ApoI r'AATT_y
Cuts at: 1493 2092 2428 2654 2806 4915 1493
Size: 599 336 226 152 2109 1697
Fragments arranged by size:
2109 1697 599 336 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3673 160
Size: 872 2641 1606
Fragments arranged by size:
2641 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5119
AvaI C'yCGr_G
Cuts at: 1870 1943 2141 2228 2444 1870
Size: 73 198 87 216 4545
Fragments arranged by size:
4545 216 198 87 73
BamHI G'GATC_C
Cuts at: 2449 2816 2449
Size: 367 4752
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2383 2440 3895 618
Size: 325 217 627 596 57 1455 1842
Fragments arranged by size:
1842 1455 627 596 325 217 57
BanII G_rGCy'C
Cuts at: 1391 2237 2246 2438 1391
Size: 846 9 192 4072
Fragments arranged by size:
4072 846 192 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4148
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3310 3344 1903
Size: 34 1373 34 3678
Fragments arranged by size:
3678 1373 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3011 4221 3011
Size: 1210 3909
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5119
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3730 136
Size: 107 122 71 631 605 2058 1525
Fragments arranged by size:
2058 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5114 5114
Size: 5119
BlpI GC'TnA_GC
Cuts at: 2195 2536 2195
Size: 341 4778
Bme1580I G_kGCm'C
Cuts at: 1790 2165 3180 4336 1790
Size: 375 1015 1156 2573
Fragments arranged by size:
2573 1156 1015 375
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5119
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2850 3808 442
Size: 416 53 83 399 24 1433 958 1753
Fragments arranged by size:
1753 1433 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5119
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3779 1239
Size: 2540 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3244 4109 4407 3244
Size: 865 298 3956
Fragments arranged by size:
3956 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3782 882
Size: 617 2283 2219
Fragments arranged by size:
2283 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2910 493
Size: 711 1706 2702
Fragments arranged by size:
2702 1706 711
BsaBI GATnn'nnATC
Cuts at: 5113 5113
Size: 5119
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2926 3308 3943 275
Size: 53 83 186 936 1393 382 635 1451
Fragments arranged by size:
1451 1393 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3552 3950 4224 4371 4782 3552
Size: 398 274 147 411 3889
Fragments arranged by size:
3889 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2333 2363 1178
Size: 30 165 30 930 30 3934
Fragments arranged by size:
3934 930 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5119
BseYI C'CCAG_C
Cuts at: 2247 2408 4322 4818 2247
Size: 161 1914 496 2548
Fragments arranged by size:
2548 1914 496 161
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2333 1163
Size: 573 597 3949
Fragments arranged by size:
3949 597 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2569 3330 4358 1540
Size: 275 186 568 761 1028 2301
Fragments arranged by size:
2301 1028 761 568 275 186
BsiHKAI G_wGCw'C
Cuts at: 2165 2237 3180 3265 4336 2165
Size: 72 943 85 1071 2948
Fragments arranged by size:
2948 1071 943 85 72
BsmI GAATG_Cn'
Cuts at: 2630 2723 2630
Size: 93 5026
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2165 2237 2246 2438 3180 3265 4336
Size: 399 375 72 9 192 742 85 1071
Cuts at: 4336 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 375 192 85 72
9
BspEI T'CCGG_A
Cuts at: 4782 4782
Size: 5119
BspHI T'CATG_A
Cuts at: 3008 4738 3008
Size: 1730 3389
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2337 2474 844
Size: 1493 137 3489
Fragments arranged by size:
3489 1493 137
BsrBI CCG'CTC
Cuts at: 1394 1823 3006 1394
Size: 429 1183 3507
Fragments arranged by size:
3507 1183 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3614 3788 65
Size: 1183 2366 174 1396
Fragments arranged by size:
2366 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3763 3950 1700
Size: 110 1953 187 2869
Fragments arranged by size:
2869 1953 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5119
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5119
BssSI C'ACGA_G
Cuts at: 3179 4191 3179
Size: 1012 4107
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2032 2155 794
Size: 1238 123 3758
Fragments arranged by size:
3758 1238 123
BstBI TT'CG_AA
Cuts at: 3923 3923
Size: 5119
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5119
BstYI r'GATC_y
Cuts at: 1972 2283 2449 2816 3201 3218 4670 4756 4768
Size: 311 166 367 385 17 1452 86 12
Cuts at: 4768 5114 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 311 166 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2395 513
Size: 552 1330 3237
Fragments arranged by size:
3237 1330 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4380
Fragments arranged by size:
4380 683 56
BtsI GCAGTG_nn'
Cuts at: 2635 3429 3449 2635
Size: 794 20 4305
Fragments arranged by size:
4305 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5119
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2038 2073 529
Size: 35 1474 35 3575
Fragments arranged by size:
3575 1474 35 35
DraI TTT'AAA
Cuts at: 2423 2423
Size: 5119
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2215 4126 809
Size: 507 899 1911 1802
Fragments arranged by size:
1911 1802 899 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2566 3455 5084
Size: 54 1425 50 275 18 736 889 1629
Cuts at: 5084 8
Size: 43
Fragments arranged by size:
1629 1425 889 736 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2566 1537
Size: 275 754 4090
Fragments arranged by size:
4090 754 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3049 1353
Size: 1696 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2254 3706 4080 4226 387
Size: 1064 138 665 1452 374 146 1280
Fragments arranged by size:
1452 1280 1064 665 374 146 138
EcoICRI GAG'CTC
Cuts at: 2235 2235
Size: 5119
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3182 3779 4566 4834 1239
Size: 393 1550 597 787 268 1524
Fragments arranged by size:
1550 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2208 2326 2208
Size: 118 5001
EcoRI G'AATT_C
Cuts at: 2092 2428 2806 2092
Size: 336 378 4405
Fragments arranged by size:
4405 378 336
FspI TGC'GCA
Cuts at: 3625 3625
Size: 5119
HaeII r_GCGC'y
Cuts at: 1678 2409 4266 1678
Size: 731 1857 2531
Fragments arranged by size:
2531 1857 731
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3760 3792 3834 3866 1373
Size: 32 135 32 2188 32 42 32 2626
Fragments arranged by size:
2626 2188 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2463 2709 669
Size: 988 281 525 246 3079
Fragments arranged by size:
3079 988 525 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5119
HpaI GTT'AAC
Cuts at: 2709 2709
Size: 5119
KpnI G_GTAC'C
Cuts at: 2444 2444
Size: 5119
MfeI C'AATT_G
Cuts at: 2718 2718
Size: 5119
MluI A'CGCG_T
Cuts at: 4995 4995
Size: 5119
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4233 4417 1305
Size: 2928 184 2007
Fragments arranged by size:
2928 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3297
Fragments arranged by size:
3297 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3077 3436 3595 518
Size: 648 1911 359 159 2042
Fragments arranged by size:
2042 1911 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2180 2317 2535 3212 4360 4605 1606
Size: 574 137 218 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 574 245 218 137
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4567
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5119
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5119
NotI GC'GGCC_GC
Cuts at: 2566 2566
Size: 5119
NspI r_CATG'y
Cuts at: 1132 1229 2477 4022 1132
Size: 97 1248 1545 2229
Fragments arranged by size:
2229 1545 1248 97
PasI CC'CwG_GG
Cuts at: 1348 2125 2296 1348
Size: 777 171 4171
Fragments arranged by size:
4171 777 171
PciI A'CATG_T
Cuts at: 4018 4018
Size: 5119
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5119
PfoI T'CCnGG_A
Cuts at: 2237 2237
Size: 5119
PmeI GTTT'AAAC
Cuts at: 2423 2423
Size: 5119
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3188 3220 4701 4733 3188
Size: 32 1481 32 3574
Fragments arranged by size:
3574 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2208 2326 2208
Size: 118 5001
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5119
PsiI TTA'TAA
Cuts at: 2689 2689
Size: 5119
PstI C_TGCA'G
Cuts at: 830 2471 830
Size: 1641 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5119
PvuII CAG'CTG
Cuts at: 2180 2180
Size: 5119
SacI G_AGCT'C
Cuts at: 2237 2237
Size: 5119
SalI G'TCGA_C
Cuts at: 1936 2461 1936
Size: 525 4594
SbfI CC_TGCA'GG
Cuts at: 2471 2471
Size: 5119
ScaI AGT'ACT
Cuts at: 3367 5079 3367
Size: 1712 3407
SfcI C'TryA_G
Cuts at: 826 1045 2467 3602 4283 4474 826
Size: 219 1422 1135 681 191 1471
Fragments arranged by size:
1471 1422 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5119
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5119
SmaI CCC'GGG
Cuts at: 1872 2143 2446 1872
Size: 271 303 4545
Fragments arranged by size:
4545 303 271
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3223 4124 4386 820
Size: 197 926 1280 901 262 1553
Fragments arranged by size:
1553 1280 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2910 493
Size: 2417 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5119
SphI G_CATG'C
Cuts at: 1132 2477 1132
Size: 1345 3774
SspI AAT'ATT
Cuts at: 5 52 3043 5
Size: 47 2991 2081
Fragments arranged by size:
2991 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5119
StyI C'CwwG_G
Cuts at: 513 1065 2558 513
Size: 552 1493 3074
Fragments arranged by size:
3074 1493 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2066 3146 3163 3316 3501 3946 325
Size: 1255 486 1080 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1080 486 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3365 5077 96
Size: 274 80 33 51 1443 1388 1712 138
Fragments arranged by size:
1712 1443 1388 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5079 5111 5079
Size: 32 5087
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5119
XbaI T'CTAG_A
Cuts at: 2096 2455 2096
Size: 359 4760
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5119
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5119
XmaI C'CCGG_G
Cuts at: 1870 2141 2444 1870
Size: 271 303 4545
Fragments arranged by size:
4545 303 271
XmnI GAAnn'nnTTC
Cuts at: 2351 3248 2351
Size: 897 4222
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2927 276
Size: 53 83 186 936 1393 2468
Fragments arranged by size:
2468 1393 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI