Restriction map for
pFN21AE5083
(Circular) (Six-base) MAPSORT of: pFN21AE5083.seq Check: 4207 from: 1 to: 7636
pFN21AE5083 7636 bp
With 182 enzymes: SgfI *
December 8, 2014 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3053 3053
Size: 7636
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3958 5446 278
Size: 53 83 186 936 2422 1488 2468
Fragments arranged by size:
2468 2422 1488 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3576 4979 1937
Size: 1639 1403 4594
Fragments arranged by size:
4594 1639 1403
Acc65I G'GTAC_C
Cuts at: 4957 4957
Size: 7636
AclI AA'CG_TT
Cuts at: 1563 5763 6136 1563
Size: 4200 373 3063
Fragments arranged by size:
4200 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2540 2759 4313 4406 4854 5699 7083 7351
Size: 908 219 1554 93 448 845 1384 268
Cuts at: 7351 1632
Size: 1917
Fragments arranged by size:
1917 1554 1384 908 845 448 268 219
93
AfeI AGC'GCT
Cuts at: 2153 2153
Size: 7636
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7439
AflIII A'CryG_T
Cuts at: 1203 6535 7512 1203
Size: 5332 977 1327
Fragments arranged by size:
5332 1327 977
AgeI A'CCGG_T
Cuts at: 2345 6467 2345
Size: 4122 3514
AhdI GACnn_n'nnGTC
Cuts at: 4612 6365 4612
Size: 1753 5883
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7636
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7636
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3384 3416 4664 4696 3384
Size: 32 1248 32 6324
Fragments arranged by size:
6324 1248 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3068 3616 3625 4331 4643 4801 5379 6951 3068
Size: 548 9 706 312 158 578 1572 3753
Fragments arranged by size:
3753 1572 706 578 548 312 158 9
ApaI G_GGCC'C
Cuts at: 4566 4566
Size: 7636
ApaLI G'TGCA_C
Cuts at: 4260 5693 6849 4260
Size: 1433 1156 5047
Fragments arranged by size:
5047 1433 1156
ApoI r'AATT_y
Cuts at: 1493 2401 3263 3425 4945 5171 5323 7432 1493
Size: 908 862 162 1520 226 152 2109 1697
Fragments arranged by size:
2109 1697 1520 908 862 226 162 152
AseI AT'TA_AT
Cuts at: 160 1032 6190 160
Size: 872 5158 1606
Fragments arranged by size:
5158 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7636
AvaI C'yCGr_G
Cuts at: 1870 1943 2389 2436 2976 3101 3696 4961 1870
Size: 73 446 47 540 125 595 1265 4545
Fragments arranged by size:
4545 1265 595 540 446 125 73 47
AvrII C'CTAG_G
Cuts at: 3920 3920
Size: 7636
BamHI G'GATC_C
Cuts at: 4966 5333 4966
Size: 367 7269
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2766 2847 3440 3673 3763
Size: 325 217 627 979 81 593 233 90
Cuts at: 3763 4957 6412 618
Size: 1194 1455 1842
Fragments arranged by size:
1842 1455 1194 979 627 593 325 233
217 90 81
BanII G_rGCy'C
Cuts at: 1391 2234 4566 4955 1391
Size: 843 2332 389 4072
Fragments arranged by size:
4072 2332 843 389
BbeI G_GCGC'C
Cuts at: 2770 2770
Size: 7636
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4076 4248 4442 928
Size: 971 2177 172 194 4122
Fragments arranged by size:
4122 2177 971 194 172
BbvCI CC'TCA_GC
Cuts at: 2479 4627 2479
Size: 2148 5488
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5827 5861 1903
Size: 34 3890 34 3678
Fragments arranged by size:
3890 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2900 5528 6738 2900
Size: 2628 1210 3798
Fragments arranged by size:
3798 2628 1210
BclI T'GATC_A
Cuts at: 1548 3109 4713 4854 1548
Size: 1561 1604 141 4330
Fragments arranged by size:
4330 1604 1561 141
BfrBI ATG'CAT
Cuts at: 4414 4414
Size: 7636
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4334 6247 136
Size: 107 122 71 631 605 2662 1913 1525
Fragments arranged by size:
2662 1913 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3895 7631 3895
Size: 3736 3900
BlpI GC'TnA_GC
Cuts at: 5053 5053
Size: 7636
Bme1580I G_kGCm'C
Cuts at: 1790 3676 4264 4566 5697 6853 1790
Size: 1886 588 302 1131 1156 2573
Fragments arranged by size:
2573 1886 1156 1131 588 302
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7636
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3773 5367 6325
Size: 416 53 83 399 24 2356 1594 958
Cuts at: 6325 442
Size: 1753
Fragments arranged by size:
2356 1753 1594 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7636
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2135 3246 3711 2135
Size: 1111 465 6060
Fragments arranged by size:
6060 1111 465
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2344 2877 3005 3078 4491 6296 1239
Size: 1105 533 128 73 1413 1805 2579
Fragments arranged by size:
2579 1805 1413 1105 533 128 73
Bpu10I CC'TnA_GC
Cuts at: 2479 4627 2479
Size: 2148 5488
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3059 4475 5761 6626 6924 3059
Size: 1416 1286 865 298 3771
Fragments arranged by size:
3771 1416 1286 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2973 3906 3975 4149 6299 882
Size: 617 1474 933 69 174 2150 2219
Fragments arranged by size:
2219 2150 1474 933 617 174 69
BsaAI yAC'GTr
Cuts at: 493 1204 5427 493
Size: 711 4223 2702
Fragments arranged by size:
4223 2702 711
BsaBI GATnn'nnATC
Cuts at: 2799 3894 7630 2799
Size: 1095 3736 2805
Fragments arranged by size:
3736 2805 1095
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2767 3565 3955 5443
Size: 53 83 186 936 1234 798 390 1488
Cuts at: 5443 5825 6460 275
Size: 382 635 1451
Fragments arranged by size:
1488 1451 1234 936 798 635 390 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2345 3123 4197 6069 6467 6741 6888 7299 2345
Size: 778 1074 1872 398 274 147 411 2682
Fragments arranged by size:
2682 1872 1074 778 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2788 2818 4192 4222 4464
Size: 30 165 30 1385 30 1374 30 242
Cuts at: 4464 4494 1178
Size: 30 4320
Fragments arranged by size:
4320 1385 1374 242 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2467 3956 4609 1186
Size: 1281 1489 653 4213
Fragments arranged by size:
4213 1489 1281 653
BseYI C'CCAG_C
Cuts at: 2541 3902 4485 4791 6839 7335 2541
Size: 1361 583 306 2048 496 2842
Fragments arranged by size:
2842 2048 1361 583 496 306
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7063
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5086 5847 6875 1540
Size: 275 186 3085 761 1028 2301
Fragments arranged by size:
3085 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2396 2724 3697 4264 5697 5782 6853 2396
Size: 328 973 567 1433 85 1071 3179
Fragments arranged by size:
3179 1433 1071 973 567 328 85
BsmI GAATG_Cn'
Cuts at: 5147 5240 5147
Size: 93 7543
BsmBI CGTCTCn'nnnn_
Cuts at: 2106 4511 2106
Size: 2405 5231
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2234 2396 2724 3676 3697 4264 4566
Size: 399 444 162 328 952 21 567 302
Cuts at: 4566 4955 5697 5782 6853 1391
Size: 389 742 85 1071 2174
Fragments arranged by size:
2174 1071 952 742 567 444 399 389
328 302 162 85 21
BspEI T'CCGG_A
Cuts at: 7299 7299
Size: 7636
BspHI T'CATG_A
Cuts at: 2578 4851 5525 7255 2578
Size: 2273 674 1730 2959
Fragments arranged by size:
2959 2273 1730 674
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3053 4991 844
Size: 2209 1938 3489
Fragments arranged by size:
3489 2209 1938
BsrBI CCG'CTC
Cuts at: 1394 1823 2212 2650 2778 4248 5523 1394
Size: 429 389 438 128 1470 1275 3507
Fragments arranged by size:
3507 1470 1275 438 429 389 128
BsrDI GCAATG_nn'
Cuts at: 65 1248 2251 2969 3309 6131 6305 65
Size: 1183 1003 718 340 2822 174 1396
Fragments arranged by size:
2822 1396 1183 1003 718 340 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2345 2730 2763 2844 2919 4611 6280
Size: 110 535 385 33 81 75 1692 1669
Cuts at: 6280 6467 1700
Size: 187 2869
Fragments arranged by size:
2869 1692 1669 535 385 187 110 81
75 33
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7636
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7636
BssSI C'ACGA_G
Cuts at: 3420 5696 6708 3420
Size: 2276 1012 4348
Fragments arranged by size:
4348 2276 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3068 3515 3616 3625 4375 4420 794
Size: 2274 447 101 9 750 45 4010
Fragments arranged by size:
4010 2274 750 447 101 45 9
BstBI TT'CG_AA
Cuts at: 6440 6440
Size: 7636
BstEII G'GTnAC_C
Cuts at: 1184 2840 4427 1184
Size: 1656 1587 4393
Fragments arranged by size:
4393 1656 1587
BstXI CCAn_nnnn'nTGG
Cuts at: 4585 4822 4585
Size: 237 7399
BstYI r'GATC_y
Cuts at: 1972 3895 4966 5333 5718 5735 7187 7273 7285
Size: 1923 1071 367 385 17 1452 86 12
Cuts at: 7285 7631 1972
Size: 346 1977
Fragments arranged by size:
1977 1923 1452 1071 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3939 3939
Size: 7636
BtgI C'CryG_G
Cuts at: 513 1065 2033 2107 2179 2203 4864 513
Size: 552 968 74 72 24 2661 3285
Fragments arranged by size:
3285 2661 968 552 74 72 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6897
Fragments arranged by size:
6897 683 56
BtsI GCAGTG_nn'
Cuts at: 4000 4275 4641 5152 5946 5966 4000
Size: 275 366 511 794 20 5670
Fragments arranged by size:
5670 794 511 366 275 20
ClaI AT'CG_AT
Cuts at: 1554 3433 4072 1554
Size: 1879 639 5118
Fragments arranged by size:
5118 1879 639
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7601
DraI TTT'AAA
Cuts at: 4689 4940 4689
Size: 251 7385
DraIII CAC_nnn'GTG
Cuts at: 3551 4781 3551
Size: 1230 6406
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6643 809
Size: 507 5327 1802
Fragments arranged by size:
5327 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2675 2694 2941
Size: 54 1425 50 275 18 845 19 247
Cuts at: 2941 3235 4824 5083 5972 7601 8
Size: 294 1589 259 889 1629 43
Fragments arranged by size:
1629 1589 1425 889 845 294 275 259
247 54 50 43 19 18
EagI C'GGCC_G
Cuts at: 1537 1812 5083 1537
Size: 275 3271 4090
Fragments arranged by size:
4090 3271 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3137 4130 4627 4901 5566 1353
Size: 1784 993 497 274 665 3423
Fragments arranged by size:
3423 1784 993 665 497 274
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2053 2305 2952 6223 6597 6743
Size: 1064 138 464 252 647 3271 374 146
Cuts at: 6743 387
Size: 1280
Fragments arranged by size:
3271 1280 1064 647 464 374 252 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2344 2540 2759 2877 3005 3078 4313
Size: 393 712 196 219 118 128 73 1235
Cuts at: 4313 4406 4491 4854 5699 6296 7083 7351 1239
Size: 93 85 363 845 597 787 268 1524
Fragments arranged by size:
1524 1235 845 787 712 597 393 363
268 219 196 128 118 93 85 73
EcoO109I rG'GnC_Cy
Cuts at: 2023 2538 2826 3392 3739 4098 4562 4759 2023
Size: 515 288 566 347 359 464 197 4900
Fragments arranged by size:
4900 566 515 464 359 347 288 197
EcoRI G'AATT_C
Cuts at: 4945 5323 4945
Size: 378 7258
EcoRV GAT'ATC
Cuts at: 3217 3217
Size: 7636
FspI TGC'GCA
Cuts at: 6142 6142
Size: 7636
HaeII r_GCGC'y
Cuts at: 1678 2155 2770 2898 4925 6783 1678
Size: 477 615 128 2027 1858 2531
Fragments arranged by size:
2531 2027 1858 615 477 128
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2522 2554 3851 3883 4192
Size: 32 135 32 950 32 1297 32 309
Cuts at: 4192 4224 4598 4630 6277 6309 6351 6383 1373
Size: 32 374 32 1647 32 42 32 2626
Fragments arranged by size:
2626 1647 1297 950 374 309 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4980 5226 669
Size: 988 281 3042 246 3079
Fragments arranged by size:
3079 3042 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7636
HpaI GTT'AAC
Cuts at: 5226 5226
Size: 7636
KasI G'GCGC_C
Cuts at: 2766 2766
Size: 7636
KpnI G_GTAC'C
Cuts at: 4961 4961
Size: 7636
MfeI C'AATT_G
Cuts at: 5235 5235
Size: 7636
MluI A'CGCG_T
Cuts at: 7512 7512
Size: 7636
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2146 4431 4509 6750 6934 1305
Size: 841 2285 78 2241 184 2007
Fragments arranged by size:
2285 2241 2007 841 184 78
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4826 10
Size: 54 1425 343 2994 2820
Fragments arranged by size:
2994 2820 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5594 5953 6112 518
Size: 648 4428 359 159 2042
Fragments arranged by size:
4428 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2035 2068 2109 2205 2259 2640 4178 4631
Size: 429 33 41 96 54 381 1538 453
Cuts at: 4631 5052 5729 6877 7122 1606
Size: 421 677 1148 245 2120
Fragments arranged by size:
2120 1538 1148 677 453 429 421 381
245 96 54 41 33
NaeI GCC'GGC
Cuts at: 2765 2765
Size: 7636
NarI GG'CG_CC
Cuts at: 2767 2767
Size: 7636
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7084
NdeI CA'TA_TG
Cuts at: 387 3531 387
Size: 3144 4492
NgoMIV G'CCGG_C
Cuts at: 2763 2763
Size: 7636
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7636
NotI GC'GGCC_GC
Cuts at: 5083 5083
Size: 7636
NsiI A_TGCA'T
Cuts at: 4416 4416
Size: 7636
NspI r_CATG'y
Cuts at: 1132 1229 2705 3518 4378 4414 4994 6539 1132
Size: 97 1476 813 860 36 580 1545 2229
Fragments arranged by size:
2229 1545 1476 860 813 580 97 36
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7636
PciI A'CATG_T
Cuts at: 6535 6535
Size: 7636
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3551 1108
Size: 2443 5193
PfoI T'CCnGG_A
Cuts at: 4800 4800
Size: 7636
PmeI GTTT'AAAC
Cuts at: 4940 4940
Size: 7636
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5705 5737 7218 7250 5705
Size: 32 1481 32 6091
Fragments arranged by size:
6091 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2023 2538 3392 3739 4759 2023
Size: 515 854 347 1020 4900
Fragments arranged by size:
4900 1020 854 515 347
PshAI GACnn'nnGTC
Cuts at: 1613 4023 1613
Size: 2410 5226
PsiI TTA'TAA
Cuts at: 3320 5206 3320
Size: 1886 5750
PspOMI G'GGCC_C
Cuts at: 4562 4562
Size: 7636
PstI C_TGCA'G
Cuts at: 830 2928 3621 3808 4988 830
Size: 2098 693 187 1180 3478
Fragments arranged by size:
3478 2098 1180 693 187
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7636
PvuII CAG'CTG
Cuts at: 4178 4631 4178
Size: 453 7183
SacII CC_GC'GG
Cuts at: 2036 2110 2206 2036
Size: 74 96 7466
Fragments arranged by size:
7466 96 74
SalI G'TCGA_C
Cuts at: 1936 4978 1936
Size: 3042 4594
SanDI GG'GwC_CC
Cuts at: 2023 2538 2023
Size: 515 7121
SapI GCTCTTCn'nnn_
Cuts at: 3137 4130 3137
Size: 993 6643
SbfI CC_TGCA'GG
Cuts at: 4988 4988
Size: 7636
ScaI AGT'ACT
Cuts at: 3247 5884 7596 3247
Size: 2637 1712 3287
Fragments arranged by size:
3287 2637 1712
SexAI A'CCwGG_T
Cuts at: 4845 4845
Size: 7636
SfcI C'TryA_G
Cuts at: 826 1045 2832 2924 3617 3804 4984 6119 6800
Size: 219 1787 92 693 187 1180 1135 681
Cuts at: 6800 6991 826
Size: 191 1471
Fragments arranged by size:
1787 1471 1180 1135 693 681 219 191
187 92
SfoI GGC'GCC
Cuts at: 2768 2768
Size: 7636
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7636
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7636
SmaI CCC'GGG
Cuts at: 1872 2438 4963 1872
Size: 566 2525 4545
Fragments arranged by size:
4545 2525 566
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2976 3038 4490 5740 6641 6903
Size: 197 926 1033 62 1452 1250 901 262
Cuts at: 6903 820
Size: 1553
Fragments arranged by size:
1553 1452 1250 1033 926 901 262 197
62
SnaBI TAC'GTA
Cuts at: 493 5427 493
Size: 4934 2702
SpeI A'CTAG_T
Cuts at: 152 3242 152
Size: 3090 4546
SphI G_CATG'C
Cuts at: 1132 2705 3518 4378 4994 1132
Size: 1573 813 860 616 3774
Fragments arranged by size:
3774 1573 860 813 616
SspI AAT'ATT
Cuts at: 5 52 5560 5
Size: 47 5508 2081
Fragments arranged by size:
5508 2081 47
StuI AGG'CCT
Cuts at: 1517 3226 3387 1517
Size: 1709 161 5766
Fragments arranged by size:
5766 1709 161
StyI C'CwwG_G
Cuts at: 513 1065 3044 3395 3870 3920 4815 5075 513
Size: 552 1979 351 475 50 895 260 3074
Fragments arranged by size:
3074 1979 895 552 475 351 260 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2282 3367 3376 3751 5663 5680 5833
Size: 1255 702 1085 9 375 1912 17 153
Cuts at: 5833 6018 6463 325
Size: 185 445 1498
Fragments arranged by size:
1912 1498 1255 1085 702 445 375 185
153 17 9
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3245 5882 7594
Size: 274 80 33 51 1443 1268 2637 1712
Cuts at: 7594 96
Size: 138
Fragments arranged by size:
2637 1712 1443 1268 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3765 3797 4464 4496 7596 7628 3765
Size: 32 667 32 3100 32 3773
Fragments arranged by size:
3773 3100 667 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7636
XbaI T'CTAG_A
Cuts at: 4972 4972
Size: 7636
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2713 4313 4357 2713
Size: 1600 44 5992
Fragments arranged by size:
5992 1600 44
XhoI C'TCGA_G
Cuts at: 1943 2976 1943
Size: 1033 6603
XmaI C'CCGG_G
Cuts at: 1870 2436 4961 1870
Size: 566 2525 4545
Fragments arranged by size:
4545 2525 566
XmnI GAAnn'nnTTC
Cuts at: 5765 5765
Size: 7636
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3956 5444 276
Size: 53 83 186 936 2422 1488 2468
Fragments arranged by size:
2468 2422 1488 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI BaeI BsiWI BstZ17I EcoICRI EcoNI FalI FseI
FspAI NruI PacI PmlI PspXI PsrI RsrII SacI
SfiI SrfI SwaI