Restriction map for
pFN21AE5082
(Circular) (Six-base) MAPSORT of: pFN21AE5082.seq Check: 1683 from: 1 to: 6754
pFN21AE5082 6754 bp
With 182 enzymes: SgfI *
December 8, 2014 13:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4564 278
Size: 53 83 186 936 3028 2468
Fragments arranged by size:
3028 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4097 1937
Size: 2160 4594
Acc65I G'GTAC_C
Cuts at: 4075 4075
Size: 6754
AclI AA'CG_TT
Cuts at: 1563 4881 5254 1563
Size: 3318 373 3063
Fragments arranged by size:
3318 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2681 4817 6201 6469 1632
Size: 1049 2136 1384 268 1917
Fragments arranged by size:
2136 1917 1384 1049 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6557
AflIII A'CryG_T
Cuts at: 1203 5653 6630 1203
Size: 4450 977 1327
Fragments arranged by size:
4450 1327 977
AgeI A'CCGG_T
Cuts at: 5585 5585
Size: 6754
AhdI GACnn_n'nnGTC
Cuts at: 5483 5483
Size: 6754
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6754
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6754
AlwNI CAG_nnn'CTG
Cuts at: 3470 4497 6069 3470
Size: 1027 1572 4155
Fragments arranged by size:
4155 1572 1027
ApaI G_GGCC'C
Cuts at: 3767 3767
Size: 6754
ApaLI G'TGCA_C
Cuts at: 4811 5967 4811
Size: 1156 5598
ApoI r'AATT_y
Cuts at: 1493 2987 3005 3435 3652 3782 4063 4289 4441
Size: 1494 18 430 217 130 281 226 152
Cuts at: 4441 6550 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1494 430 281 226 217 152
130 18
AseI AT'TA_AT
Cuts at: 160 1032 5308 160
Size: 872 4276 1606
Fragments arranged by size:
4276 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6754
AvaI C'yCGr_G
Cuts at: 1870 1943 2943 4079 1870
Size: 73 1000 1136 4545
Fragments arranged by size:
4545 1136 1000 73
BamHI G'GATC_C
Cuts at: 4084 4451 4084
Size: 367 6387
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4075 5530 618
Size: 325 217 627 2288 1455 1842
Fragments arranged by size:
2288 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2405 3511 3767 4073 1391
Size: 1014 1106 256 306 4072
Fragments arranged by size:
4072 1106 1014 306 256
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2060 2919 3615 928
Size: 971 161 859 696 4067
Fragments arranged by size:
4067 971 859 696 161
BbvCI CC'TCA_GC
Cuts at: 2508 2508
Size: 6754
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4945 4979 1903
Size: 34 3008 34 3678
Fragments arranged by size:
3678 3008 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4646 5856 4646
Size: 1210 5544
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6754
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2654 5365 136
Size: 107 122 71 631 605 982 2711 1525
Fragments arranged by size:
2711 1525 982 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2304 6749 2304
Size: 4445 2309
BlpI GC'TnA_GC
Cuts at: 2483 3021 4171 2483
Size: 538 1150 5066
Fragments arranged by size:
5066 1150 538
Bme1580I G_kGCm'C
Cuts at: 1790 3767 4815 5971 1790
Size: 1977 1048 1156 2573
Fragments arranged by size:
2573 1977 1156 1048
BmgBI CAC'GTC
Cuts at: 1318 3502 1318
Size: 2184 4570
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4021 4485 5443
Size: 416 53 83 399 24 2604 464 958
Cuts at: 5443 442
Size: 1753
Fragments arranged by size:
2604 1753 958 464 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6754
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2408 3068 3267 3402 5414 1239
Size: 1169 660 199 135 2012 2579
Fragments arranged by size:
2579 2012 1169 660 199 135
Bpu10I CC'TnA_GC
Cuts at: 2508 2508
Size: 6754
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4879 5744 6042 4879
Size: 865 298 5591
Fragments arranged by size:
5591 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5417 882
Size: 617 3918 2219
Fragments arranged by size:
3918 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4545 493
Size: 711 3341 2702
Fragments arranged by size:
3341 2702 711
BsaBI GATnn'nnATC
Cuts at: 6748 6748
Size: 6754
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4561 4943 5578 275
Size: 53 83 186 936 3028 382 635 1451
Fragments arranged by size:
3028 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3924 5187 5585 5859 6006 6417 3924
Size: 1263 398 274 147 411 4261
Fragments arranged by size:
4261 1263 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6529
Fragments arranged by size:
6529 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2369 2480 3405 3869 1186
Size: 1183 111 925 464 4071
Fragments arranged by size:
4071 1183 925 464 111
BseYI C'CCAG_C
Cuts at: 2649 2964 3270 3400 3664 3695 5957 6453 2649
Size: 315 306 130 264 31 2262 496 2950
Fragments arranged by size:
2950 2262 496 315 306 264 130 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3713 1163
Size: 573 1977 4204
Fragments arranged by size:
4204 1977 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4204 4965 5993 1540
Size: 275 186 2203 761 1028 2301
Fragments arranged by size:
2301 2203 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3511 4815 4900 5971 3511
Size: 1304 85 1071 4294
Fragments arranged by size:
4294 1304 1071 85
BsmI GAATG_Cn'
Cuts at: 2236 4265 4358 2236
Size: 2029 93 4632
Fragments arranged by size:
4632 2029 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2968 2968
Size: 6754
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2405 3511 3767 4073 4815 4900 5971
Size: 399 615 1106 256 306 742 85 1071
Cuts at: 5971 1391
Size: 2174
Fragments arranged by size:
2174 1106 1071 742 615 399 306 256
85
BspEI T'CCGG_A
Cuts at: 6417 6417
Size: 6754
BspHI T'CATG_A
Cuts at: 2589 4643 6373 2589
Size: 2054 1730 2970
Fragments arranged by size:
2970 2054 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4109 844
Size: 3265 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4641 1394
Size: 429 2818 3507
Fragments arranged by size:
3507 2818 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2686 2721 2952 3120 5249 5423 65
Size: 1183 1438 35 231 168 2129 174 1396
Fragments arranged by size:
2129 1438 1396 1183 231 174 168 35
BsrFI r'CCGG_y
Cuts at: 1700 1810 5398 5585 1700
Size: 110 3588 187 2869
Fragments arranged by size:
3588 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6754
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6754
BssSI C'ACGA_G
Cuts at: 3975 4814 5826 3975
Size: 839 1012 4903
Fragments arranged by size:
4903 1012 839
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6754
BstBI TT'CG_AA
Cuts at: 3649 5558 3649
Size: 1909 4845
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6754
BstXI CCAn_nnnn'nTGG
Cuts at: 2692 2692
Size: 6754
BstYI r'GATC_y
Cuts at: 1972 2304 4084 4451 4836 4853 6305 6391 6403
Size: 332 1780 367 385 17 1452 86 12
Cuts at: 6403 6749 1972
Size: 346 1977
Fragments arranged by size:
1977 1780 1452 385 367 346 332 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2042 2715 513
Size: 552 939 38 673 4552
Fragments arranged by size:
4552 939 673 552 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6015
Fragments arranged by size:
6015 683 56
BtsI GCAGTG_nn'
Cuts at: 2257 4270 5064 5084 2257
Size: 2013 794 20 3927
Fragments arranged by size:
3927 2013 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6754
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6719
DraI TTT'AAA
Cuts at: 2986 4058 2986
Size: 1072 5682
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2945 5761 809
Size: 507 1629 2816 1802
Fragments arranged by size:
2816 1802 1629 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4201 5090 6719
Size: 54 1425 50 275 18 2371 889 1629
Cuts at: 6719 8
Size: 43
Fragments arranged by size:
2371 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4201 1537
Size: 275 2389 4090
Fragments arranged by size:
4090 2389 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2057 2361 2864 3534 4684 1353
Size: 704 304 503 670 1150 3423
Fragments arranged by size:
3423 1150 704 670 503 304
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5341 5715 5861 387
Size: 1064 138 3752 374 146 1280
Fragments arranged by size:
3752 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3509 3509
Size: 6754
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2408 2681 3068 3267 3402 4817 5414
Size: 393 776 273 387 199 135 1415 597
Cuts at: 5414 6201 6469 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1415 787 776 597 393 387 273
268 199 135
EcoO109I rG'GnC_Cy
Cuts at: 2068 3763 3764 4015 2068
Size: 1695 1 251 4807
Fragments arranged by size:
4807 1695 251 1
EcoRI G'AATT_C
Cuts at: 4063 4441 4063
Size: 378 6376
FspI TGC'GCA
Cuts at: 5260 5260
Size: 6754
HaeII r_GCGC'y
Cuts at: 1678 2971 5901 1678
Size: 1293 2930 2531
Fragments arranged by size:
2930 2531 1293
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2906 2938 3025 3057 3400
Size: 32 135 32 1334 32 87 32 343
Cuts at: 3400 3432 3842 3874 5395 5427 5469 5501 1373
Size: 32 410 32 1521 32 42 32 2626
Fragments arranged by size:
2626 1521 1334 410 343 135 87 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3289 3742 4098 4344 669
Size: 988 281 1351 453 356 246 3079
Fragments arranged by size:
3079 1351 988 453 356 281 246
HindIII A'AGCT_T
Cuts at: 748 3599 748
Size: 2851 3903
HpaI GTT'AAC
Cuts at: 4344 4344
Size: 6754
KpnI G_GTAC'C
Cuts at: 4079 4079
Size: 6754
MfeI C'AATT_G
Cuts at: 4353 4353
Size: 6754
MluI A'CGCG_T
Cuts at: 6630 6630
Size: 6754
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5868 6052 1305
Size: 4563 184 2007
Fragments arranged by size:
4563 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4932
Fragments arranged by size:
4932 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2588 3735 4712 5071 5230 518
Size: 648 1422 1147 977 359 159 2042
Fragments arranged by size:
2042 1422 1147 977 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2482 2624 2653 4170 4847 5995 6240 1606
Size: 876 142 29 1517 677 1148 245 2120
Fragments arranged by size:
2120 1517 1148 876 677 245 142 29
NcoI C'CATG_G
Cuts at: 513 1065 2004 2042 2715 513
Size: 552 939 38 673 4552
Fragments arranged by size:
4552 939 673 552 38
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6754
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6754
NotI GC'GGCC_GC
Cuts at: 4201 4201
Size: 6754
NspI r_CATG'y
Cuts at: 1132 1229 2413 2839 4112 5657 1132
Size: 97 1184 426 1273 1545 2229
Fragments arranged by size:
2229 1545 1273 1184 426 97
PasI CC'CwG_GG
Cuts at: 1348 2384 1348
Size: 1036 5718
PciI A'CATG_T
Cuts at: 5653 5653
Size: 6754
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2716 1108
Size: 1608 5146
PfoI T'CCnGG_A
Cuts at: 2775 2775
Size: 6754
PmeI GTTT'AAAC
Cuts at: 4058 4058
Size: 6754
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3929 3961 4823 4855 6336 6368 3929
Size: 32 862 32 1481 32 4315
Fragments arranged by size:
4315 1481 862 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2068 4015 2068
Size: 1947 4807
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6754
PsiI TTA'TAA
Cuts at: 4324 4324
Size: 6754
PspOMI G'GGCC_C
Cuts at: 3763 3763
Size: 6754
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3630 3662 3630
Size: 32 6722
PstI C_TGCA'G
Cuts at: 830 4106 830
Size: 3276 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6754
PvuII CAG'CTG
Cuts at: 2482 2624 2653 2482
Size: 142 29 6583
Fragments arranged by size:
6583 142 29
SacI G_AGCT'C
Cuts at: 3511 3511
Size: 6754
SalI G'TCGA_C
Cuts at: 1936 4096 1936
Size: 2160 4594
SapI GCTCTTCn'nnn_
Cuts at: 2361 2361
Size: 6754
SbfI CC_TGCA'GG
Cuts at: 4106 4106
Size: 6754
ScaI AGT'ACT
Cuts at: 5002 6714 5002
Size: 1712 5042
SfcI C'TryA_G
Cuts at: 826 1045 2796 3056 3960 4102 5237 5918 6109
Size: 219 1751 260 904 142 1135 681 191
Cuts at: 6109 826
Size: 1471
Fragments arranged by size:
1751 1471 1135 904 681 260 219 191
142
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6754
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6754
SmaI CCC'GGG
Cuts at: 1872 4081 1872
Size: 2209 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4858 5759 6021 820
Size: 197 926 2915 901 262 1553
Fragments arranged by size:
2915 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4545 493
Size: 4052 2702
SpeI A'CTAG_T
Cuts at: 152 3898 152
Size: 3746 3008
SphI G_CATG'C
Cuts at: 1132 2413 2839 4112 1132
Size: 1281 426 1273 3774
Fragments arranged by size:
3774 1281 1273 426
SspI AAT'ATT
Cuts at: 5 52 2857 4678 5
Size: 47 2805 1821 2081
Fragments arranged by size:
2805 2081 1821 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6754
StyI C'CwwG_G
Cuts at: 513 1065 2004 2042 2715 3768 4193 513
Size: 552 939 38 673 1053 425 3074
Fragments arranged by size:
3074 1053 939 673 552 425 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4781 4798 4951 5136 5581 325
Size: 1255 3201 17 153 185 445 1498
Fragments arranged by size:
3201 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3895 5000 6712
Size: 274 80 33 51 1443 1918 1105 1712
Cuts at: 6712 96
Size: 138
Fragments arranged by size:
1918 1712 1443 1105 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2757 2789 6714 6746 2757
Size: 32 3925 32 2765
Fragments arranged by size:
3925 2765 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6754
XbaI T'CTAG_A
Cuts at: 4090 4090
Size: 6754
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6754
XmaI C'CCGG_G
Cuts at: 1870 4079 1870
Size: 2209 4545
XmnI GAAnn'nnTTC
Cuts at: 2544 3471 4883 2544
Size: 927 1412 4415
Fragments arranged by size:
4415 1412 927
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4562 276
Size: 53 83 186 936 3028 2468
Fragments arranged by size:
3028 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI