Restriction map for
pFN21AE5079
(Circular) (Six-base) MAPSORT of: pFN21AE5079.seq Check: 3211 from: 1 to: 5782
pFN21AE5079 5782 bp
With 182 enzymes: SgfI *
December 8, 2014 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2732 2732
Size: 5782
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3592 278
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2831 3125 1937
Size: 894 294 4594
Fragments arranged by size:
4594 894 294
Acc65I G'GTAC_C
Cuts at: 3103 3103
Size: 5782
AclI AA'CG_TT
Cuts at: 1563 3909 4282 1563
Size: 2346 373 3063
Fragments arranged by size:
3063 2346 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2211 2355 3845 5229 5497 1632
Size: 579 144 1490 1384 268 1917
Fragments arranged by size:
1917 1490 1384 579 268 144
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5585
AflIII A'CryG_T
Cuts at: 1203 4681 5658 1203
Size: 3478 977 1327
Fragments arranged by size:
3478 1327 977
AgeI A'CCGG_T
Cuts at: 4613 4613
Size: 5782
AhdI GACnn_n'nnGTC
Cuts at: 4511 4511
Size: 5782
AleI CACnn'nnGTG
Cuts at: 1166 2744 1166
Size: 1578 4204
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2939 1184
Size: 1755 4027
AlwNI CAG_nnn'CTG
Cuts at: 2333 3525 5097 2333
Size: 1192 1572 3018
Fragments arranged by size:
3018 1572 1192
ApaLI G'TGCA_C
Cuts at: 3839 4995 3839
Size: 1156 4626
ApoI r'AATT_y
Cuts at: 1493 2854 3091 3317 3469 5578 1493
Size: 1361 237 226 152 2109 1697
Fragments arranged by size:
2109 1697 1361 237 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4336 160
Size: 872 3304 1606
Fragments arranged by size:
3304 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
AvaI C'yCGr_G
Cuts at: 1870 1943 2698 3107 1870
Size: 73 755 409 4545
Fragments arranged by size:
4545 755 409 73
BamHI G'GATC_C
Cuts at: 3112 3479 3112
Size: 367 5415
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3064 3103 4558 618
Size: 325 217 627 1277 39 1455 1842
Fragments arranged by size:
1842 1455 1277 627 325 217 39
BanII G_rGCy'C
Cuts at: 1391 2080 2447 2991 3101 1391
Size: 689 367 544 110 4072
Fragments arranged by size:
4072 689 544 367 110
BbeI G_GCGC'C
Cuts at: 3068 3068
Size: 5782
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2299 2638 928
Size: 971 400 339 4072
Fragments arranged by size:
4072 971 400 339
BbvCI CC'TCA_GC
Cuts at: 2928 2928
Size: 5782
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3973 4007 1903
Size: 34 2036 34 3678
Fragments arranged by size:
3678 2036 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3674 4884 3674
Size: 1210 4572
BclI T'GATC_A
Cuts at: 1548 2148 1548
Size: 600 5182
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4393 136
Size: 107 122 71 631 605 2721 1525
Fragments arranged by size:
2721 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5777 5777
Size: 5782
BlpI GC'TnA_GC
Cuts at: 3005 3199 3005
Size: 194 5588
Bme1580I G_kGCm'C
Cuts at: 1790 2241 3843 4999 1790
Size: 451 1602 1156 2573
Fragments arranged by size:
2573 1602 1156 451
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5782
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2465 3513 4471
Size: 416 53 83 399 24 1048 1048 958
Cuts at: 4471 442
Size: 1753
Fragments arranged by size:
1753 1048 1048 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5782
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2270 2566 4442 1239
Size: 1031 296 1876 2579
Fragments arranged by size:
2579 1876 1031 296
Bpu10I CC'TnA_GC
Cuts at: 2301 2681 2764 2928 2301
Size: 380 83 164 5155
Fragments arranged by size:
5155 380 164 83
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3907 4772 5070 3907
Size: 865 298 4619
Fragments arranged by size:
4619 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2953 4445 882
Size: 617 1454 1492 2219
Fragments arranged by size:
2219 1492 1454 617
BsaAI yAC'GTr
Cuts at: 493 1204 3573 493
Size: 711 2369 2702
Fragments arranged by size:
2702 2369 711
BsaBI GATnn'nnATC
Cuts at: 5776 5776
Size: 5782
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3065 3589 3971 4606
Size: 53 83 186 936 1532 524 382 635
Cuts at: 4606 275
Size: 1451
Fragments arranged by size:
1532 1451 936 635 524 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3013 4215 4613 4887 5034 5445 3013
Size: 1202 398 274 147 411 3350
Fragments arranged by size:
3350 1202 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5557
Fragments arranged by size:
5557 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 2546 1186
Size: 847 513 4422
Fragments arranged by size:
4422 847 513
BseYI C'CCAG_C
Cuts at: 2626 4985 5481 2626
Size: 2359 496 2927
Fragments arranged by size:
2927 2359 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2846 2918 1163
Size: 573 1110 72 4027
Fragments arranged by size:
4027 1110 573 72
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3232 3993 5021 1540
Size: 275 186 1231 761 1028 2301
Fragments arranged by size:
2301 1231 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3843 3928 4999 3843
Size: 85 1071 4626
Fragments arranged by size:
4626 1071 85
BsmI GAATG_Cn'
Cuts at: 3009 3293 3386 3009
Size: 284 93 5405
Fragments arranged by size:
5405 284 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2080 2241 2447 2991 3101 3843 3928
Size: 399 290 161 206 544 110 742 85
Cuts at: 3928 4999 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 544 399 290 206 161
110 85
BspEI T'CCGG_A
Cuts at: 5445 5445
Size: 5782
BspHI T'CATG_A
Cuts at: 3671 5401 3671
Size: 1730 4052
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2732 3137 844
Size: 1888 405 3489
Fragments arranged by size:
3489 1888 405
BsrBI CCG'CTC
Cuts at: 1394 1823 3669 1394
Size: 429 1846 3507
Fragments arranged by size:
3507 1846 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4277 4451 65
Size: 1183 3029 174 1396
Fragments arranged by size:
3029 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4426 4613 1700
Size: 110 2616 187 2869
Fragments arranged by size:
2869 2616 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5782
BssHII G'CGCG_C
Cuts at: 1925 3058 1925
Size: 1133 4649
BssSI C'ACGA_G
Cuts at: 2144 3842 4854 2144
Size: 1698 1012 3072
Fragments arranged by size:
3072 1698 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5782
BstBI TT'CG_AA
Cuts at: 4586 4586
Size: 5782
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5782
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2248 2011
Size: 237 5545
BstYI r'GATC_y
Cuts at: 1972 3112 3479 3864 3881 5333 5419 5431 5777
Size: 1140 367 385 17 1452 86 12 346
Cuts at: 5777 1972
Size: 1977
Fragments arranged by size:
1977 1452 1140 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2832 2832
Size: 5782
BtgI C'CryG_G
Cuts at: 513 1065 2004 3069 513
Size: 552 939 1065 3226
Fragments arranged by size:
3226 1065 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2578 487
Size: 683 56 1352 3691
Fragments arranged by size:
3691 1352 683 56
BtsI GCAGTG_nn'
Cuts at: 2322 2739 2751 3298 4092 4112 2322
Size: 417 12 547 794 20 3992
Fragments arranged by size:
3992 794 547 417 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5782
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5747
DraI TTT'AAA
Cuts at: 3086 3086
Size: 5782
DraIII CAC_nnn'GTG
Cuts at: 2437 2437
Size: 5782
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4789 809
Size: 507 3473 1802
Fragments arranged by size:
3473 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3229 4118 5747
Size: 54 1425 50 275 18 1399 889 1629
Cuts at: 5747 8
Size: 43
Fragments arranged by size:
1629 1425 1399 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3229 1537
Size: 275 1417 4090
Fragments arranged by size:
4090 1417 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2230 2951 3712 1353
Size: 877 721 761 3423
Fragments arranged by size:
3423 877 761 721
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2765 4369 4743 4889 387
Size: 1064 138 1176 1604 374 146 1280
Fragments arranged by size:
1604 1280 1176 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2211 2270 2355 2566 3845 4442 5229
Size: 393 579 59 85 211 1279 597 787
Cuts at: 5229 5497 1239
Size: 268 1524
Fragments arranged by size:
1524 1279 787 597 579 393 268 211
85 59
EcoNI CCTnn'n_nnAGG
Cuts at: 2300 2300
Size: 5782
EcoO109I rG'GnC_Cy
Cuts at: 3036 3036
Size: 5782
EcoRI G'AATT_C
Cuts at: 2854 3091 3469 2854
Size: 237 378 5167
Fragments arranged by size:
5167 378 237
EcoRV GAT'ATC
Cuts at: 2578 2578
Size: 5782
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2360 2360
Size: 5782
FspI TGC'GCA
Cuts at: 3043 4288 3043
Size: 1245 4537
HaeII r_GCGC'y
Cuts at: 1678 3068 4929 1678
Size: 1390 1861 2531
Fragments arranged by size:
2531 1861 1390
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2534 2566 4423 4455 4497
Size: 32 135 32 962 32 1857 32 42
Cuts at: 4497 4529 1373
Size: 32 2626
Fragments arranged by size:
2626 1857 962 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3126 3372 669
Size: 988 281 1188 246 3079
Fragments arranged by size:
3079 1188 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5782
HpaI GTT'AAC
Cuts at: 3372 3372
Size: 5782
KasI G'GCGC_C
Cuts at: 3064 3064
Size: 5782
KpnI G_GTAC'C
Cuts at: 3107 3107
Size: 5782
MfeI C'AATT_G
Cuts at: 3381 3381
Size: 5782
MluI A'CGCG_T
Cuts at: 5658 5658
Size: 5782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4896 5080 1305
Size: 3591 184 2007
Fragments arranged by size:
3591 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3960
Fragments arranged by size:
3960 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2744 2846 3740 4099 4258 518
Size: 648 1578 102 894 359 159 2042
Fragments arranged by size:
2042 1578 894 648 359 159 102
MspA1I CmG'CkG
Cuts at: 1606 2422 2932 3198 3875 5023 5268 1606
Size: 816 510 266 677 1148 245 2120
Fragments arranged by size:
2120 1148 816 677 510 266 245
NarI GG'CG_CC
Cuts at: 3065 3065
Size: 5782
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4291
Fragments arranged by size:
4291 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5782
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5782
NotI GC'GGCC_GC
Cuts at: 3229 3229
Size: 5782
NspI r_CATG'y
Cuts at: 1132 1229 3048 3140 4685 1132
Size: 97 1819 92 1545 2229
Fragments arranged by size:
2229 1819 1545 97 92
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5782
PciI A'CATG_T
Cuts at: 4681 4681
Size: 5782
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5782
PfoI T'CCnGG_A
Cuts at: 2907 2907
Size: 5782
PmeI GTTT'AAAC
Cuts at: 3086 3086
Size: 5782
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3851 3883 5364 5396 3851
Size: 32 1481 32 4237
Fragments arranged by size:
4237 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2916 1613
Size: 1303 4479
PsiI TTA'TAA
Cuts at: 3352 3352
Size: 5782
PstI C_TGCA'G
Cuts at: 830 2329 2746 3134 830
Size: 1499 417 388 3478
Fragments arranged by size:
3478 1499 417 388
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5782
PvuII CAG'CTG
Cuts at: 2422 2932 2422
Size: 510 5272
SalI G'TCGA_C
Cuts at: 1936 3124 1936
Size: 1188 4594
SbfI CC_TGCA'GG
Cuts at: 3134 3134
Size: 5782
ScaI AGT'ACT
Cuts at: 4030 5742 4030
Size: 1712 4070
SfcI C'TryA_G
Cuts at: 826 1045 2325 2533 2742 2835 2866 3130 4265
Size: 219 1280 208 209 93 31 264 1135
Cuts at: 4265 4946 5137 826
Size: 681 191 1471
Fragments arranged by size:
1471 1280 1135 681 264 219 209 208
191 93 31
SfoI GGC'GCC
Cuts at: 3066 3066
Size: 5782
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5782
SmaI CCC'GGG
Cuts at: 1872 3109 1872
Size: 1237 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3886 4787 5049 820
Size: 197 926 1943 901 262 1553
Fragments arranged by size:
1943 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3573 493
Size: 3080 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5782
SphI G_CATG'C
Cuts at: 1132 3048 3140 1132
Size: 1916 92 3774
Fragments arranged by size:
3774 1916 92
SspI AAT'ATT
Cuts at: 5 52 3706 5
Size: 47 3654 2081
Fragments arranged by size:
3654 2081 47
StuI AGG'CCT
Cuts at: 1517 2714 1517
Size: 1197 4585
StyI C'CwwG_G
Cuts at: 513 1065 2004 2010 2047 3031 3221 513
Size: 552 939 6 37 984 190 3074
Fragments arranged by size:
3074 984 939 552 190 37 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3809 3826 3979 4164 4609 325
Size: 1255 2229 17 153 185 445 1498
Fragments arranged by size:
2229 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4028 5740 96
Size: 274 80 33 51 1443 2051 1712 138
Fragments arranged by size:
2051 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5742 5774 5742
Size: 32 5750
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5782
XbaI T'CTAG_A
Cuts at: 3118 3118
Size: 5782
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5782
XmaI C'CCGG_G
Cuts at: 1870 3107 1870
Size: 1237 4545
XmnI GAAnn'nnTTC
Cuts at: 3911 3911
Size: 5782
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3590 276
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI Bsu36I EcoICRI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI XcmI