Restriction map for
pFN21AE5059
(Circular) (Six-base) MAPSORT of: pFN21AE5059.seq Check: 6654 from: 1 to: 5626
pFN21AE5059 5626 bp
With 182 enzymes: SgfI *
December 8, 2014 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2625 2625
Size: 5626
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3436 278
Size: 53 83 186 936 1900 2468
Fragments arranged by size:
2468 1900 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2969 1937
Size: 1032 4594
Acc65I G'GTAC_C
Cuts at: 2947 2947
Size: 5626
AclI AA'CG_TT
Cuts at: 1563 3753 4126 1563
Size: 2190 373 3063
Fragments arranged by size:
3063 2190 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3689 5073 5341 1632
Size: 2057 1384 268 1917
Fragments arranged by size:
2057 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5429
AflIII A'CryG_T
Cuts at: 1203 2469 2589 4525 5502 1203
Size: 1266 120 1936 977 1327
Fragments arranged by size:
1936 1327 1266 977 120
AgeI A'CCGG_T
Cuts at: 4457 4457
Size: 5626
AhdI GACnn_n'nnGTC
Cuts at: 4355 4355
Size: 5626
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5626
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2078 1184
Size: 894 4732
AlwNI CAG_nnn'CTG
Cuts at: 3369 4941 3369
Size: 1572 4054
ApaLI G'TGCA_C
Cuts at: 3683 4839 3683
Size: 1156 4470
ApoI r'AATT_y
Cuts at: 1493 2935 3161 3313 5422 1493
Size: 1442 226 152 2109 1697
Fragments arranged by size:
2109 1697 1442 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4180 160
Size: 872 3148 1606
Fragments arranged by size:
3148 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5626
AvaI C'yCGr_G
Cuts at: 1870 1943 2115 2126 2161 2398 2951 1870
Size: 73 172 11 35 237 553 4545
Fragments arranged by size:
4545 553 237 172 73 35 11
BamHI G'GATC_C
Cuts at: 2956 3323 2956
Size: 367 5259
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2062 2111 2233 2414 2460
Size: 325 217 627 275 49 122 181 46
Cuts at: 2460 2947 4402 618
Size: 487 1455 1842
Fragments arranged by size:
1842 1455 627 487 325 275 217 181
122 49 46
BanII G_rGCy'C
Cuts at: 1391 2217 2399 2945 1391
Size: 826 182 546 4072
Fragments arranged by size:
4072 826 546 182
BbeI G_GCGC'C
Cuts at: 2115 2237 2418 2115
Size: 122 181 5323
Fragments arranged by size:
5323 181 122
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4655
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2731 2765 3817 3851 1903
Size: 34 50 34 710 34 1052 34 3678
Fragments arranged by size:
3678 1052 710 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3518 4728 3518
Size: 1210 4416
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5626
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2885 2918 4237
Size: 107 122 71 631 605 1213 33 1319
Cuts at: 4237 136
Size: 1525
Fragments arranged by size:
1525 1319 1213 631 605 122 107 71
33
BglII A'GATC_T
Cuts at: 5621 5621
Size: 5626
BlpI GC'TnA_GC
Cuts at: 3043 3043
Size: 5626
Bme1580I G_kGCm'C
Cuts at: 1790 2463 3687 4843 1790
Size: 673 1224 1156 2573
Fragments arranged by size:
2573 1224 1156 673
BmgBI CAC'GTC
Cuts at: 1318 2502 2574 1318
Size: 1184 72 4370
Fragments arranged by size:
4370 1184 72
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3357 4315 442
Size: 416 53 83 399 24 1940 958 1753
Fragments arranged by size:
1940 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5626
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2216 4286 1239
Size: 977 2070 2579
Fragments arranged by size:
2579 2070 977
Bpu10I CC'TnA_GC
Cuts at: 2513 2513
Size: 5626
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2825 3751 4616 4914 2825
Size: 926 865 298 3537
Fragments arranged by size:
3537 926 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4289 882
Size: 617 2790 2219
Fragments arranged by size:
2790 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2101 2590 3417 493
Size: 711 897 489 827 2702
Fragments arranged by size:
2702 897 827 711 489
BsaBI GATnn'nnATC
Cuts at: 5620 5620
Size: 5626
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2112 2234 2331 2415
Size: 53 83 186 936 579 122 97 84
Cuts at: 2415 2649 3433 3815 4450 275
Size: 234 784 382 635 1451
Fragments arranged by size:
1451 936 784 635 579 382 234 186
122 97 84 83 53
BsaWI w'CCGG_w
Cuts at: 4059 4457 4731 4878 5289 4059
Size: 398 274 147 411 4396
Fragments arranged by size:
4396 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2306 2336 1178
Size: 30 165 30 903 30 4468
Fragments arranged by size:
4468 903 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2255 1186
Size: 1069 4557
BseYI C'CCAG_C
Cuts at: 4829 5325 4829
Size: 496 5130
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2074 1163
Size: 573 338 4715
Fragments arranged by size:
4715 573 338
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2211 2758 2889 3076 3837 4865
Size: 275 186 210 547 131 187 761 1028
Cuts at: 4865 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 547 275 210 187 186
131
BsiHKAI G_wGCw'C
Cuts at: 3687 3772 4843 3687
Size: 85 1071 4470
Fragments arranged by size:
4470 1071 85
BsmI GAATG_Cn'
Cuts at: 3137 3230 3137
Size: 93 5533
BsmBI CGTCTCn'nnnn_
Cuts at: 2397 2397
Size: 5626
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2217 2399 2463 2945 3687 3772 4843
Size: 399 427 182 64 482 742 85 1071
Cuts at: 4843 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 482 427 399 182 85
64
BspEI T'CCGG_A
Cuts at: 5289 5289
Size: 5626
BspHI T'CATG_A
Cuts at: 3515 5245 3515
Size: 1730 3896
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2625 2640 2775 2981 844
Size: 1781 15 135 206 3489
Fragments arranged by size:
3489 1781 206 135 15
BsrBI CCG'CTC
Cuts at: 1394 1823 2760 3513 1394
Size: 429 937 753 3507
Fragments arranged by size:
3507 937 753 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4121 4295 65
Size: 1183 2873 174 1396
Fragments arranged by size:
2873 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2065 2227 2463 2708 2912 4270 4457
Size: 110 255 162 236 245 204 1358 187
Cuts at: 4457 1700
Size: 2869
Fragments arranged by size:
2869 1358 255 245 236 204 187 162
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5626
BssHII G'CGCG_C
Cuts at: 1925 2282 2425 2434 2558 2730 2770 2772 1925
Size: 357 143 9 124 172 40 2 4779
Fragments arranged by size:
4779 357 172 143 124 40 9 2
BssSI C'ACGA_G
Cuts at: 3686 4698 3686
Size: 1012 4614
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2155 2632 794
Size: 1361 477 3788
Fragments arranged by size:
3788 1361 477
BstBI TT'CG_AA
Cuts at: 4430 4430
Size: 5626
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5626
BstYI r'GATC_y
Cuts at: 1972 2956 3323 3708 3725 5177 5263 5275 5621
Size: 984 367 385 17 1452 86 12 346
Cuts at: 5621 1972
Size: 1977
Fragments arranged by size:
1977 1452 984 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2325 513
Size: 552 1260 3814
Fragments arranged by size:
3814 1260 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4887
Fragments arranged by size:
4887 683 56
BtsI GCAGTG_nn'
Cuts at: 2313 2734 2860 3142 3936 3956 2313
Size: 421 126 282 794 20 3983
Fragments arranged by size:
3983 794 421 282 126 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5626
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5591
DraI TTT'AAA
Cuts at: 2930 2930
Size: 5626
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4633 809
Size: 507 3317 1802
Fragments arranged by size:
3317 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2208 2886 2919
Size: 54 1425 50 275 18 378 678 33
Cuts at: 2919 3073 3962 5591 8
Size: 154 889 1629 43
Fragments arranged by size:
1629 1425 889 678 378 275 154 54
50 43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 2208 2886 3073 1537
Size: 275 396 678 187 4090
Fragments arranged by size:
4090 678 396 275 187
EarI CTCTTCn'nnn_
Cuts at: 1353 3556 1353
Size: 2203 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4213 4587 4733 387
Size: 1064 138 2624 374 146 1280
Fragments arranged by size:
2624 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2216 3689 4286 5073 5341 1239
Size: 393 584 1473 597 787 268 1524
Fragments arranged by size:
1524 1473 787 597 584 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2120 2120
Size: 5626
EcoRI G'AATT_C
Cuts at: 2935 3313 2935
Size: 378 5248
FspI TGC'GCA
Cuts at: 4132 4132
Size: 5626
HaeII r_GCGC'y
Cuts at: 1678 2115 2237 2356 2418 4773 1678
Size: 437 122 119 62 2355 2531
Fragments arranged by size:
2531 2355 437 122 119 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2306 2338 2339 2371 4267
Size: 32 135 32 734 32 1 32 1896
Cuts at: 4267 4299 4341 4373 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1896 734 135 42 32 32 32
32 32 32 1
HincII GTy'rAC
Cuts at: 669 1657 1938 2970 3216 669
Size: 988 281 1032 246 3079
Fragments arranged by size:
3079 1032 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5626
HpaI GTT'AAC
Cuts at: 3216 3216
Size: 5626
KasI G'GCGC_C
Cuts at: 2111 2233 2414 2111
Size: 122 181 5323
Fragments arranged by size:
5323 181 122
KpnI G_GTAC'C
Cuts at: 2951 2951
Size: 5626
MfeI C'AATT_G
Cuts at: 3225 3225
Size: 5626
MluI A'CGCG_T
Cuts at: 2469 5502 2469
Size: 3033 2593
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2787 4740 4924 1305
Size: 1482 1953 184 2007
Fragments arranged by size:
2007 1953 1482 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3804
Fragments arranged by size:
3804 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3584 3943 4102 518
Size: 648 2418 359 159 2042
Fragments arranged by size:
2418 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2058 2554 2811 3042 3719 4867 5112 1606
Size: 452 496 257 231 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 496 452 257 245 231
NaeI GCC'GGC
Cuts at: 2067 2229 2914 2067
Size: 162 685 4779
Fragments arranged by size:
4779 685 162
NarI GG'CG_CC
Cuts at: 2112 2234 2415 2112
Size: 122 181 5323
Fragments arranged by size:
5323 181 122
NcoI C'CATG_G
Cuts at: 513 1065 2325 513
Size: 552 1260 3814
Fragments arranged by size:
3814 1260 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5626
NgoMIV G'CCGG_C
Cuts at: 2065 2227 2912 2065
Size: 162 685 4779
Fragments arranged by size:
4779 685 162
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5626
NotI GC'GGCC_GC
Cuts at: 3073 3073
Size: 5626
NspI r_CATG'y
Cuts at: 1132 1229 2698 2782 2984 4529 1132
Size: 97 1469 84 202 1545 2229
Fragments arranged by size:
2229 1545 1469 202 97 84
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5626
PciI A'CATG_T
Cuts at: 4525 4525
Size: 5626
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2086 1108
Size: 978 4648
PmeI GTTT'AAAC
Cuts at: 2930 2930
Size: 5626
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3695 3727 5208 5240 3695
Size: 32 1481 32 4081
Fragments arranged by size:
4081 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5626
PsiI TTA'TAA
Cuts at: 3196 3196
Size: 5626
PstI C_TGCA'G
Cuts at: 830 2688 2978 830
Size: 1858 290 3478
Fragments arranged by size:
3478 1858 290
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5626
PvuII CAG'CTG
Cuts at: 2554 2554
Size: 5626
SalI G'TCGA_C
Cuts at: 1936 2968 1936
Size: 1032 4594
SbfI CC_TGCA'GG
Cuts at: 2978 2978
Size: 5626
ScaI AGT'ACT
Cuts at: 3874 5586 3874
Size: 1712 3914
SfcI C'TryA_G
Cuts at: 826 1045 2684 2974 4109 4790 4981 826
Size: 219 1639 290 1135 681 191 1471
Fragments arranged by size:
1639 1471 1135 681 290 219 191
SfoI GGC'GCC
Cuts at: 2113 2235 2416 2113
Size: 122 181 5323
Fragments arranged by size:
5323 181 122
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5626
SgrAI Cr'CCGG_yG
Cuts at: 1700 2227 2708 1700
Size: 527 481 4618
Fragments arranged by size:
4618 527 481
SmaI CCC'GGG
Cuts at: 1872 2117 2128 2953 1872
Size: 245 11 825 4545
Fragments arranged by size:
4545 825 245 11
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2840 3730 4631 4893 820
Size: 197 926 897 890 901 262 1553
Fragments arranged by size:
1553 926 901 897 890 262 197
SnaBI TAC'GTA
Cuts at: 493 3417 493
Size: 2924 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5626
SphI G_CATG'C
Cuts at: 1132 2984 1132
Size: 1852 3774
SspI AAT'ATT
Cuts at: 5 52 3550 5
Size: 47 3498 2081
Fragments arranged by size:
3498 2081 47
StuI AGG'CCT
Cuts at: 1517 2585 2663 2747 1517
Size: 1068 78 84 4396
Fragments arranged by size:
4396 1068 84 78
StyI C'CwwG_G
Cuts at: 513 1065 2085 2325 2880 3065 513
Size: 552 1020 240 555 185 3074
Fragments arranged by size:
3074 1020 555 552 240 185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2327 3653 3670 3823 4008 4453 325
Size: 1255 747 1326 17 153 185 445 1498
Fragments arranged by size:
1498 1326 1255 747 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2244 3872 5584
Size: 274 80 33 51 1443 267 1628 1712
Cuts at: 5584 96
Size: 138
Fragments arranged by size:
1712 1628 1443 274 267 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5586 5618 5586
Size: 32 5594
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5626
XbaI T'CTAG_A
Cuts at: 2962 2962
Size: 5626
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5626
XmaI C'CCGG_G
Cuts at: 1870 2115 2126 2951 1870
Size: 245 11 825 4545
Fragments arranged by size:
4545 825 245 11
XmnI GAAnn'nnTTC
Cuts at: 3755 3755
Size: 5626
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3434 276
Size: 53 83 186 936 1900 2468
Fragments arranged by size:
2468 1900 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SrfI SwaI XcmI