Restriction map for
pFN21AE5056
(Circular) (Six-base) MAPSORT of: pFN21AE5056.seq Check: 8375 from: 1 to: 5377
pFN21AE5056 5377 bp
With 182 enzymes: SgfI *
December 8, 2014 13:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3187 278
Size: 53 83 186 936 1651 2468
Fragments arranged by size:
2468 1651 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2461 2720 1937
Size: 524 259 4594
Fragments arranged by size:
4594 524 259
Acc65I G'GTAC_C
Cuts at: 2698 2698
Size: 5377
AclI AA'CG_TT
Cuts at: 1563 3504 3877 1563
Size: 1941 373 3063
Fragments arranged by size:
3063 1941 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2522 2531 2693 3440 4824 5092 1632
Size: 890 9 162 747 1384 268 1917
Fragments arranged by size:
1917 1384 890 747 268 162 9
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5180
AflIII A'CryG_T
Cuts at: 1203 4276 5253 1203
Size: 3073 977 1327
Fragments arranged by size:
3073 1327 977
AgeI A'CCGG_T
Cuts at: 4208 4208
Size: 5377
AhdI GACnn_n'nnGTC
Cuts at: 4106 4106
Size: 5377
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5377
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2307 1184
Size: 1123 4254
AlwNI CAG_nnn'CTG
Cuts at: 2385 3120 4692 2385
Size: 735 1572 3070
Fragments arranged by size:
3070 1572 735
ApaLI G'TGCA_C
Cuts at: 3434 4590 3434
Size: 1156 4221
ApoI r'AATT_y
Cuts at: 1493 2162 2572 2686 2912 3064 5173 1493
Size: 669 410 114 226 152 2109 1697
Fragments arranged by size:
2109 1697 669 410 226 152 114
AseI AT'TA_AT
Cuts at: 160 1032 3931 160
Size: 872 2899 1606
Fragments arranged by size:
2899 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5377
AvaI C'yCGr_G
Cuts at: 1870 1943 2262 2702 1870
Size: 73 319 440 4545
Fragments arranged by size:
4545 440 319 73
BamHI G'GATC_C
Cuts at: 2707 3074 2707
Size: 367 5010
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2698 4153 618
Size: 325 217 627 911 1455 1842
Fragments arranged by size:
1842 1455 911 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2696 1391
Size: 1305 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4406
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2243 2277 3568 3602 1903
Size: 34 306 34 1291 34 3678
Fragments arranged by size:
3678 1291 306 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2261 3269 4479 2261
Size: 1008 1210 3159
Fragments arranged by size:
3159 1210 1008
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5377
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2039 3988 136
Size: 107 122 71 631 605 367 1949 1525
Fragments arranged by size:
1949 1525 631 605 367 122 107 71
BglII A'GATC_T
Cuts at: 5372 5372
Size: 5377
BlpI GC'TnA_GC
Cuts at: 2794 2794
Size: 5377
Bme1580I G_kGCm'C
Cuts at: 1790 3438 4594 1790
Size: 1648 1156 2573
Fragments arranged by size:
2573 1648 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5377
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3108 4066 442
Size: 416 53 83 399 24 1691 958 1753
Fragments arranged by size:
1753 1691 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5377
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2062 2062
Size: 5377
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2087 2362 4037 1239
Size: 848 275 1675 2579
Fragments arranged by size:
2579 1675 848 275
Bpu10I CC'TnA_GC
Cuts at: 2420 2420
Size: 5377
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3502 4367 4665 3502
Size: 865 298 4214
Fragments arranged by size:
4214 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4040 882
Size: 617 2541 2219
Fragments arranged by size:
2541 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3168 493
Size: 711 1964 2702
Fragments arranged by size:
2702 1964 711
BsaBI GATnn'nnATC
Cuts at: 5371 5371
Size: 5377
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3184 3566 4201 275
Size: 53 83 186 936 1651 382 635 1451
Fragments arranged by size:
1651 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2172 3810 4208 4482 4629 5040 2172
Size: 1638 398 274 147 411 2509
Fragments arranged by size:
2509 1638 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5152
Fragments arranged by size:
5152 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2492 2666 1186
Size: 1306 174 3897
Fragments arranged by size:
3897 1306 174
BseYI C'CCAG_C
Cuts at: 2341 4580 5076 2341
Size: 2239 496 2642
Fragments arranged by size:
2642 2239 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2273 2409 1163
Size: 573 537 136 4131
Fragments arranged by size:
4131 573 537 136
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2827 3588 4616 1540
Size: 275 186 826 761 1028 2301
Fragments arranged by size:
2301 1028 826 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3438 3523 4594 3438
Size: 85 1071 4221
Fragments arranged by size:
4221 1071 85
BsmI GAATG_Cn'
Cuts at: 2888 2981 2888
Size: 93 5284
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2696 3438 3523 4594 1391
Size: 399 906 742 85 1071 2174
Fragments arranged by size:
2174 1071 906 742 399 85
BspEI T'CCGG_A
Cuts at: 2172 5040 2172
Size: 2868 2509
BspHI T'CATG_A
Cuts at: 2538 3266 4996 2538
Size: 728 1730 2919
Fragments arranged by size:
2919 1730 728
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2445 2732 844
Size: 1601 287 3489
Fragments arranged by size:
3489 1601 287
BsrBI CCG'CTC
Cuts at: 1394 1823 3264 1394
Size: 429 1441 3507
Fragments arranged by size:
3507 1441 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3872 4046 65
Size: 1183 2624 174 1396
Fragments arranged by size:
2624 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4021 4208 1700
Size: 110 2211 187 2869
Fragments arranged by size:
2869 2211 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5377
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5377
BssSI C'ACGA_G
Cuts at: 3437 4449 3437
Size: 1012 4365
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2396 794
Size: 1602 3775
BstBI TT'CG_AA
Cuts at: 2556 4181 2556
Size: 1625 3752
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5377
BstYI r'GATC_y
Cuts at: 1972 2062 2487 2707 3074 3459 3476 4928 5014
Size: 90 425 220 367 385 17 1452 86
Cuts at: 5014 5026 5372 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 425 385 367 346 220 90
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3886
Fragments arranged by size:
3886 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4638
Fragments arranged by size:
4638 683 56
BtsI GCAGTG_nn'
Cuts at: 2893 3687 3707 2893
Size: 794 20 4563
Fragments arranged by size:
4563 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5377
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5342
DraI TTT'AAA
Cuts at: 2161 2681 2161
Size: 520 4857
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4384 809
Size: 507 3068 1802
Fragments arranged by size:
3068 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2031 2824 3713
Size: 54 1425 50 275 18 201 793 889
Cuts at: 3713 5342 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 793 275 201 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2824 1537
Size: 275 1012 4090
Fragments arranged by size:
4090 1012 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2013 2471 2583 3307 1353
Size: 660 458 112 724 3423
Fragments arranged by size:
3423 724 660 458 112
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3964 4338 4484 387
Size: 1064 138 2375 374 146 1280
Fragments arranged by size:
2375 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 2362 2522 2531 2693 3440 4037
Size: 393 455 275 160 9 162 747 597
Cuts at: 4037 4824 5092 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 747 597 455 393 275 268
162 160 9
EcoRI G'AATT_C
Cuts at: 2686 3064 2686
Size: 378 4999
FspI TGC'GCA
Cuts at: 2534 3883 2534
Size: 1349 4028
HaeII r_GCGC'y
Cuts at: 1678 4524 1678
Size: 2846 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2582 2614 4018 4050 4092
Size: 32 135 32 1010 32 1404 32 42
Cuts at: 4092 4124 1373
Size: 32 2626
Fragments arranged by size:
2626 1404 1010 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2462 2721 2967 669
Size: 988 281 524 259 246 3079
Fragments arranged by size:
3079 988 524 281 259 246
HindIII A'AGCT_T
Cuts at: 748 2307 748
Size: 1559 3818
HpaI GTT'AAC
Cuts at: 2967 2967
Size: 5377
KpnI G_GTAC'C
Cuts at: 2702 2702
Size: 5377
MfeI C'AATT_G
Cuts at: 2976 2976
Size: 5377
MluI A'CGCG_T
Cuts at: 5253 5253
Size: 5377
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4491 4675 1305
Size: 3186 184 2007
Fragments arranged by size:
3186 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2033 10
Size: 54 1425 343 201 3354
Fragments arranged by size:
3354 1425 343 201 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2453 3335 3694 3853 518
Size: 648 1287 882 359 159 2042
Fragments arranged by size:
2042 1287 882 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2793 3470 4618 4863 1606
Size: 1187 677 1148 245 2120
Fragments arranged by size:
2120 1187 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3886
Fragments arranged by size:
3886 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5377
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5377
NotI GC'GGCC_GC
Cuts at: 2824 2824
Size: 5377
NspI r_CATG'y
Cuts at: 1132 1229 2288 2735 4280 1132
Size: 97 1059 447 1545 2229
Fragments arranged by size:
2229 1545 1059 447 97
PasI CC'CwG_GG
Cuts at: 1348 2616 1348
Size: 1268 4109
PciI A'CATG_T
Cuts at: 4276 4276
Size: 5377
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5377
PfoI T'CCnGG_A
Cuts at: 2192 2192
Size: 5377
PmeI GTTT'AAAC
Cuts at: 2681 2681
Size: 5377
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3446 3478 4959 4991 3446
Size: 32 1481 32 3832
Fragments arranged by size:
3832 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5377
PsiI TTA'TAA
Cuts at: 2947 2947
Size: 5377
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2627 2659 2627
Size: 32 5345
PstI C_TGCA'G
Cuts at: 830 2729 830
Size: 1899 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5377
SalI G'TCGA_C
Cuts at: 1936 2460 2719 1936
Size: 524 259 4594
Fragments arranged by size:
4594 524 259
SbfI CC_TGCA'GG
Cuts at: 2729 2729
Size: 5377
ScaI AGT'ACT
Cuts at: 3625 5337 3625
Size: 1712 3665
SexAI A'CCwGG_T
Cuts at: 2363 2363
Size: 5377
SfcI C'TryA_G
Cuts at: 826 1045 2725 3860 4541 4732 826
Size: 219 1680 1135 681 191 1471
Fragments arranged by size:
1680 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5377
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5377
SmaI CCC'GGG
Cuts at: 1872 2704 1872
Size: 832 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3481 4382 4644 820
Size: 197 926 1538 901 262 1553
Fragments arranged by size:
1553 1538 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3168 493
Size: 2675 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5377
SphI G_CATG'C
Cuts at: 1132 2288 2735 1132
Size: 1156 447 3774
Fragments arranged by size:
3774 1156 447
SspI AAT'ATT
Cuts at: 5 52 3301 5
Size: 47 3249 2081
Fragments arranged by size:
3249 2081 47
StuI AGG'CCT
Cuts at: 1517 2606 1517
Size: 1089 4288
StyI C'CwwG_G
Cuts at: 513 1065 2004 2601 2816 513
Size: 552 939 597 215 3074
Fragments arranged by size:
3074 939 597 552 215
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3404 3421 3574 3759 4204 325
Size: 1255 1824 17 153 185 445 1498
Fragments arranged by size:
1824 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2184 3623 5335
Size: 274 80 33 51 1443 207 1439 1712
Cuts at: 5335 96
Size: 138
Fragments arranged by size:
1712 1443 1439 274 207 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5337 5369 5337
Size: 32 5345
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5377
XbaI T'CTAG_A
Cuts at: 2713 2713
Size: 5377
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5377
XmaI C'CCGG_G
Cuts at: 1870 2702 1870
Size: 832 4545
XmnI GAAnn'nnTTC
Cuts at: 3506 3506
Size: 5377
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3185 276
Size: 53 83 186 936 1651 2468
Fragments arranged by size:
2468 1651 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI
SfiI SfoI SrfI SwaI XcmI