Restriction map for
pFN21AE5053
(Circular) (Six-base) MAPSORT of: pFN21AE5053.seq Check: 4599 from: 1 to: 5212
pFN21AE5053 5212 bp
With 182 enzymes: SgfI *
December 8, 2014 13:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3022 278
Size: 53 83 186 936 1486 2468
Fragments arranged by size:
2468 1486 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2555 1937
Size: 618 4594
Acc65I G'GTAC_C
Cuts at: 2533 2533
Size: 5212
AclI AA'CG_TT
Cuts at: 1563 3339 3712 1563
Size: 1776 373 3063
Fragments arranged by size:
3063 1776 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3275 4659 4927 1632
Size: 1643 1384 268 1917
Fragments arranged by size:
1917 1643 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5015
AflIII A'CryG_T
Cuts at: 1203 4111 5088 1203
Size: 2908 977 1327
Fragments arranged by size:
2908 1327 977
AgeI A'CCGG_T
Cuts at: 4043 4043
Size: 5212
AhdI GACnn_n'nnGTC
Cuts at: 3941 3941
Size: 5212
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5212
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5212
AlwNI CAG_nnn'CTG
Cuts at: 2155 2955 4527 2155
Size: 800 1572 2840
Fragments arranged by size:
2840 1572 800
ApaI G_GGCC'C
Cuts at: 2391 2391
Size: 5212
ApaLI G'TGCA_C
Cuts at: 2060 2114 2264 3269 4425 2060
Size: 54 150 1005 1156 2847
Fragments arranged by size:
2847 1156 1005 150 54
ApoI r'AATT_y
Cuts at: 1493 2521 2747 2899 5008 1493
Size: 1028 226 152 2109 1697
Fragments arranged by size:
2109 1697 1028 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3766 160
Size: 872 2734 1606
Fragments arranged by size:
2734 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5212
AvaI C'yCGr_G
Cuts at: 1870 1943 2301 2537 1870
Size: 73 358 236 4545
Fragments arranged by size:
4545 358 236 73
BamHI G'GATC_C
Cuts at: 2542 2909 2542
Size: 367 4845
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2011 2533 3988 618
Size: 325 217 627 224 522 1455 1842
Fragments arranged by size:
1842 1455 627 522 325 224 217
BanII G_rGCy'C
Cuts at: 1391 2283 2391 2531 1391
Size: 892 108 140 4072
Fragments arranged by size:
4072 892 140 108
BbeI G_GCGC'C
Cuts at: 2015 2015
Size: 5212
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4241
BbvCI CC'TCA_GC
Cuts at: 2370 2370
Size: 5212
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3403 3437 1903
Size: 34 1466 34 3678
Fragments arranged by size:
3678 1466 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3104 4314 3104
Size: 1210 4002
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5212
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3823 136
Size: 107 122 71 631 605 337 1814 1525
Fragments arranged by size:
1814 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5207 5207
Size: 5212
BlpI GC'TnA_GC
Cuts at: 2307 2376 2629 2307
Size: 69 253 4890
Fragments arranged by size:
4890 253 69
Bme1580I G_kGCm'C
Cuts at: 1790 2064 2118 2268 2391 3273 4429 1790
Size: 274 54 150 123 882 1156 2573
Fragments arranged by size:
2573 1156 882 274 150 123 54
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5212
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2186 2466 2943
Size: 416 53 83 399 24 769 280 477
Cuts at: 2943 3901 442
Size: 958 1753
Fragments arranged by size:
1753 958 769 477 416 399 280 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5212
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2102 2195 2468 3872 1239
Size: 863 93 273 1404 2579
Fragments arranged by size:
2579 1404 863 273 93
Bpu10I CC'TnA_GC
Cuts at: 2370 2370
Size: 5212
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3337 4202 4500 3337
Size: 865 298 4049
Fragments arranged by size:
4049 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3875 882
Size: 617 2376 2219
Fragments arranged by size:
2376 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3003 493
Size: 711 1799 2702
Fragments arranged by size:
2702 1799 711
BsaBI GATnn'nnATC
Cuts at: 5206 5206
Size: 5212
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 3019 3401 4036
Size: 53 83 186 936 479 1007 382 635
Cuts at: 4036 275
Size: 1451
Fragments arranged by size:
1451 1007 936 635 479 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3645 4043 4317 4464 4875 3645
Size: 398 274 147 411 3982
Fragments arranged by size:
3982 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4987
Fragments arranged by size:
4987 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5212
BseYI C'CCAG_C
Cuts at: 2348 4415 4911 2348
Size: 2067 496 2649
Fragments arranged by size:
2649 2067 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4639
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2662 3423 4451 1540
Size: 275 186 661 761 1028 2301
Fragments arranged by size:
2301 1028 761 661 275 186
BsiHKAI G_wGCw'C
Cuts at: 2064 2118 2268 2283 3273 3358 4429 2064
Size: 54 150 15 990 85 1071 2847
Fragments arranged by size:
2847 1071 990 150 85 54 15
BsmI GAATG_Cn'
Cuts at: 2723 2816 2723
Size: 93 5119
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2118 2268 2283 2391 2531 3273
Size: 399 274 54 150 15 108 140 742
Cuts at: 3273 3358 4429 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 274 150 140 108
85 54 15
BspEI T'CCGG_A
Cuts at: 4875 4875
Size: 5212
BspHI T'CATG_A
Cuts at: 3101 4831 3101
Size: 1730 3482
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2567 844
Size: 1723 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3099 1394
Size: 429 1276 3507
Fragments arranged by size:
3507 1276 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3707 3881 65
Size: 1183 2459 174 1396
Fragments arranged by size:
2459 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2017 2064 2334 2395 3856 4043 1700
Size: 110 207 47 270 61 1461 187 2869
Fragments arranged by size:
2869 1461 270 207 187 110 61 47
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5212
BssHII G'CGCG_C
Cuts at: 1925 2068 2158 1925
Size: 143 90 4979
Fragments arranged by size:
4979 143 90
BssSI C'ACGA_G
Cuts at: 3272 4284 3272
Size: 1012 4200
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5212
BstBI TT'CG_AA
Cuts at: 4016 4016
Size: 5212
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5212
BstYI r'GATC_y
Cuts at: 1972 2028 2542 2909 3294 3311 4763 4849 4861
Size: 56 514 367 385 17 1452 86 12
Cuts at: 4861 5207 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 514 385 367 346 86 56
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4660
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4473
Fragments arranged by size:
4473 683 56
BtsI GCAGTG_nn'
Cuts at: 2499 2728 3522 3542 2499
Size: 229 794 20 4169
Fragments arranged by size:
4169 794 229 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5212
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5177
DraI TTT'AAA
Cuts at: 2516 2516
Size: 5212
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4219 809
Size: 507 2903 1802
Fragments arranged by size:
2903 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2336 2397 2410
Size: 54 1425 50 275 18 506 61 13
Cuts at: 2410 2659 3548 5177 8
Size: 249 889 1629 43
Fragments arranged by size:
1629 1425 889 506 275 249 61 54
50 43 18 13
EagI C'GGCC_G
Cuts at: 1537 1812 2659 1537
Size: 275 847 4090
Fragments arranged by size:
4090 847 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2457 3142 1353
Size: 1104 685 3423
Fragments arranged by size:
3423 1104 685
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2117 2483 3799 4173 4319 387
Size: 1064 138 528 366 1316 374 146 1280
Fragments arranged by size:
1316 1280 1064 528 374 366 146 138
EcoICRI GAG'CTC
Cuts at: 2281 2281
Size: 5212
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2102 2195 2468 3275 3872 4659 4927
Size: 393 470 93 273 807 597 787 268
Cuts at: 4927 1239
Size: 1524
Fragments arranged by size:
1524 807 787 597 470 393 273 268
93
EcoNI CCTnn'n_nnAGG
Cuts at: 2369 2369
Size: 5212
EcoO109I rG'GnC_Cy
Cuts at: 2387 2388 2387
Size: 1 5211
EcoRI G'AATT_C
Cuts at: 2521 2899 2521
Size: 378 4834
FspI TGC'GCA
Cuts at: 3718 3718
Size: 5212
HaeII r_GCGC'y
Cuts at: 1678 2015 2134 2383 4359 1678
Size: 337 119 249 1976 2531
Fragments arranged by size:
2531 1976 337 249 119
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3853 3885 3927 3959 1373
Size: 32 135 32 2281 32 42 32 2626
Fragments arranged by size:
2626 2281 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2556 2802 669
Size: 988 281 618 246 3079
Fragments arranged by size:
3079 988 618 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5212
HpaI GTT'AAC
Cuts at: 2802 2802
Size: 5212
KasI G'GCGC_C
Cuts at: 2011 2011
Size: 5212
KpnI G_GTAC'C
Cuts at: 2537 2537
Size: 5212
MfeI C'AATT_G
Cuts at: 2811 2811
Size: 5212
MluI A'CGCG_T
Cuts at: 5088 5088
Size: 5212
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4326 4510 1305
Size: 3021 184 2007
Fragments arranged by size:
3021 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3390
Fragments arranged by size:
3390 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3170 3529 3688 518
Size: 648 2004 359 159 2042
Fragments arranged by size:
2042 2004 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2075 2155 2221 2628 3305 4453 4698 1606
Size: 469 80 66 407 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 469 407 245 80 66
NaeI GCC'GGC
Cuts at: 2019 2336 2397 2019
Size: 317 61 4834
Fragments arranged by size:
4834 317 61
NarI GG'CG_CC
Cuts at: 2012 2012
Size: 5212
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4660
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5212
NgoMIV G'CCGG_C
Cuts at: 2017 2334 2395 2017
Size: 317 61 4834
Fragments arranged by size:
4834 317 61
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5212
NotI GC'GGCC_GC
Cuts at: 2659 2659
Size: 5212
NspI r_CATG'y
Cuts at: 1132 1229 2570 4115 1132
Size: 97 1341 1545 2229
Fragments arranged by size:
2229 1545 1341 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5212
PciI A'CATG_T
Cuts at: 4111 4111
Size: 5212
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5212
PfoI T'CCnGG_A
Cuts at: 2031 2211 2031
Size: 180 5032
PmeI GTTT'AAAC
Cuts at: 2516 2516
Size: 5212
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3281 3313 4794 4826 3281
Size: 32 1481 32 3667
Fragments arranged by size:
3667 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5212
PsiI TTA'TAA
Cuts at: 2782 2782
Size: 5212
PspOMI G'GGCC_C
Cuts at: 2387 2387
Size: 5212
PstI C_TGCA'G
Cuts at: 830 2028 2256 2319 2494 2564 830
Size: 1198 228 63 175 70 3478
Fragments arranged by size:
3478 1198 228 175 70 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5212
PvuII CAG'CTG
Cuts at: 2155 2221 2155
Size: 66 5146
SacI G_AGCT'C
Cuts at: 2283 2283
Size: 5212
SalI G'TCGA_C
Cuts at: 1936 2554 1936
Size: 618 4594
SbfI CC_TGCA'GG
Cuts at: 2256 2564 2256
Size: 308 4904
ScaI AGT'ACT
Cuts at: 3460 5172 3460
Size: 1712 3500
SfcI C'TryA_G
Cuts at: 826 1045 2024 2252 2315 2490 2560 3695 4376
Size: 219 979 228 63 175 70 1135 681
Cuts at: 4376 4567 826
Size: 191 1471
Fragments arranged by size:
1471 1135 979 681 228 219 191 175
70 63
SfoI GGC'GCC
Cuts at: 2013 2013
Size: 5212
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5212
SgrAI Cr'CCGG_yG
Cuts at: 1700 2064 1700
Size: 364 4848
SmaI CCC'GGG
Cuts at: 1872 2303 2539 1872
Size: 431 236 4545
Fragments arranged by size:
4545 431 236
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3316 4217 4479 820
Size: 197 926 1373 901 262 1553
Fragments arranged by size:
1553 1373 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3003 493
Size: 2510 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5212
SphI G_CATG'C
Cuts at: 1132 2570 1132
Size: 1438 3774
SspI AAT'ATT
Cuts at: 5 52 3136 5
Size: 47 3084 2081
Fragments arranged by size:
3084 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5212
StyI C'CwwG_G
Cuts at: 513 1065 2187 2651 513
Size: 552 1122 464 3074
Fragments arranged by size:
3074 1122 552 464
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2430 3239 3256 3409 3594 4039 325
Size: 1255 850 809 17 153 185 445 1498
Fragments arranged by size:
1498 1255 850 809 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3458 5170 96
Size: 274 80 33 51 1443 1481 1712 138
Fragments arranged by size:
1712 1481 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5172 5204 5172
Size: 32 5180
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5212
XbaI T'CTAG_A
Cuts at: 2548 2548
Size: 5212
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5212
XmaI C'CCGG_G
Cuts at: 1870 2301 2537 1870
Size: 431 236 4545
Fragments arranged by size:
4545 431 236
XmnI GAAnn'nnTTC
Cuts at: 3341 3341
Size: 5212
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3020 276
Size: 53 83 186 936 1486 2468
Fragments arranged by size:
2468 1486 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI
FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI
SwaI XcmI