Restriction map for
pFN21AE5023
(Circular) (Six-base) MAPSORT of: pFN21AE5023.seq Check: 3372 from: 1 to: 6106
pFN21AE5023 6106 bp
With 182 enzymes: SgfI *
December 8, 2014 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3145 3145
Size: 6106
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3916 278
Size: 53 83 186 936 2380 2468
Fragments arranged by size:
2468 2380 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3449 1937
Size: 1512 4594
Acc65I G'GTAC_C
Cuts at: 3427 3427
Size: 6106
AclI AA'CG_TT
Cuts at: 1563 4233 4606 1563
Size: 2670 373 3063
Fragments arranged by size:
3063 2670 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4169 5553 5821 1632
Size: 2537 1384 268 1917
Fragments arranged by size:
2537 1917 1384 268
AfeI AGC'GCT
Cuts at: 2357 2357
Size: 6106
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5909
AflIII A'CryG_T
Cuts at: 1203 2796 5005 5982 1203
Size: 1593 2209 977 1327
Fragments arranged by size:
2209 1593 1327 977
AgeI A'CCGG_T
Cuts at: 4937 4937
Size: 6106
AhdI GACnn_n'nnGTC
Cuts at: 4835 4835
Size: 6106
AleI CACnn'nnGTG
Cuts at: 1166 2579 1166
Size: 1413 4693
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6106
AlwNI CAG_nnn'CTG
Cuts at: 3849 5421 3849
Size: 1572 4534
ApaI G_GGCC'C
Cuts at: 2294 3347 2294
Size: 1053 5053
ApaLI G'TGCA_C
Cuts at: 3026 4163 5319 3026
Size: 1137 1156 3813
Fragments arranged by size:
3813 1156 1137
ApoI r'AATT_y
Cuts at: 1493 3415 3641 3793 5902 1493
Size: 1922 226 152 2109 1697
Fragments arranged by size:
2109 1922 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2345 2912 2345
Size: 567 5539
AseI AT'TA_AT
Cuts at: 160 1032 4660 160
Size: 872 3628 1606
Fragments arranged by size:
3628 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6106
AvaI C'yCGr_G
Cuts at: 1870 1943 2026 2463 2521 2827 3149 3281 3431
Size: 73 83 437 58 306 322 132 150
Cuts at: 3431 1870
Size: 4545
Fragments arranged by size:
4545 437 322 306 150 132 83 73
58
BamHI G'GATC_C
Cuts at: 3436 3803 3436
Size: 367 5739
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2490 3427 4882 618
Size: 325 217 627 703 937 1455 1842
Fragments arranged by size:
1842 1455 937 703 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2294 2444 3252 3347 3425 1391
Size: 903 150 808 95 78 4072
Fragments arranged by size:
4072 903 808 150 95 78
BbeI G_GCGC'C
Cuts at: 2494 2494
Size: 6106
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5135
BbvCI CC'TCA_GC
Cuts at: 3179 3179
Size: 6106
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2524 2558 2644 2678 4297 4331 1903
Size: 34 587 34 86 34 1619 34 3678
Fragments arranged by size:
3678 1619 587 86 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3998 5208 3998
Size: 1210 4896
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6106
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2111 2252 2576
Size: 107 122 71 631 605 439 141 324
Cuts at: 2576 4717 136
Size: 2141 1525
Fragments arranged by size:
2141 1525 631 605 439 324 141 122
107 71
BglII A'GATC_T
Cuts at: 6101 6101
Size: 6106
BlpI GC'TnA_GC
Cuts at: 3167 3523 3167
Size: 356 5750
Bme1580I G_kGCm'C
Cuts at: 1790 2294 3030 3078 3347 4167 5323 1790
Size: 504 736 48 269 820 1156 2573
Fragments arranged by size:
2573 1156 820 736 504 269 48
BmgBI CAC'GTC
Cuts at: 1318 2062 2197 2503 1318
Size: 744 135 306 4921
Fragments arranged by size:
4921 744 306 135
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2338 3837 4795
Size: 416 53 83 399 24 921 1499 958
Cuts at: 4795 442
Size: 1753
Fragments arranged by size:
1753 1499 958 921 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6106
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3352 3352
Size: 6106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2372 2690 2867 3052 4766 1239
Size: 917 216 318 177 185 1714 2579
Fragments arranged by size:
2579 1714 917 318 216 185 177
Bpu10I CC'TnA_GC
Cuts at: 2594 3179 2594
Size: 585 5521
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3236 4231 5096 5394 3236
Size: 995 865 298 3948
Fragments arranged by size:
3948 995 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2666 4769 882
Size: 617 1167 2103 2219
Fragments arranged by size:
2219 2103 1167 617
BsaAI yAC'GTr
Cuts at: 493 1204 2797 2899 3897 493
Size: 711 1593 102 998 2702
Fragments arranged by size:
2702 1593 998 711 102
BsaBI GATnn'nnATC
Cuts at: 6100 6100
Size: 6106
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2491 3913 4295 4930
Size: 53 83 186 936 958 1422 382 635
Cuts at: 4930 275
Size: 1451
Fragments arranged by size:
1451 1422 958 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4539 4937 5211 5358 5769 4539
Size: 398 274 147 411 4876
Fragments arranged by size:
4876 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2663 2693 1178
Size: 30 165 30 1260 30 4591
Fragments arranged by size:
4591 1260 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2402 2405 2636 2639 1186
Size: 1216 3 231 3 4653
Fragments arranged by size:
4653 1216 231 3 3
BseYI C'CCAG_C
Cuts at: 2809 2923 2938 3046 5309 5805 2809
Size: 114 15 108 2263 496 3110
Fragments arranged by size:
3110 2263 496 114 108 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5533
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2224 2400 2485 2580 3556 4317
Size: 275 186 223 176 85 95 976 761
Cuts at: 4317 5345 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 976 761 275 223 186 176
95 85
BsiHKAI G_wGCw'C
Cuts at: 2600 2642 2895 3030 3252 4167 4252 5323 2600
Size: 42 253 135 222 915 85 1071 3383
Fragments arranged by size:
3383 1071 915 253 222 135 85 42
BsmI GAATG_Cn'
Cuts at: 3617 3710 3617
Size: 93 6013
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2294 2444 2600 2642 2895 3030 3078
Size: 399 504 150 156 42 253 135 48
Cuts at: 3078 3252 3347 3425 4167 4252 5323 1391
Size: 174 95 78 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 504 399 253 174 156
150 135 95 85 78 48 42
BspEI T'CCGG_A
Cuts at: 5769 5769
Size: 6106
BspHI T'CATG_A
Cuts at: 3995 5725 3995
Size: 1730 4376
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2178 3145 3461 844
Size: 1334 967 316 3489
Fragments arranged by size:
3489 1334 967 316
BsrBI CCG'CTC
Cuts at: 1394 1823 2629 3993 1394
Size: 429 806 1364 3507
Fragments arranged by size:
3507 1364 806 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4601 4775 65
Size: 1183 3353 174 1396
Fragments arranged by size:
3353 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2484 3286 4750 4937 1700
Size: 110 674 802 1464 187 2869
Fragments arranged by size:
2869 1464 802 674 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6106
BssHII G'CGCG_C
Cuts at: 1925 2129 2146 2263 2298 2345 2447 2449 2749
Size: 204 17 117 35 47 102 2 300
Cuts at: 2749 2751 2851 2912 2961 2963 3051 3053 3268
Size: 2 100 61 49 2 88 2 215
Cuts at: 3268 1925
Size: 4763
Fragments arranged by size:
4763 300 215 204 117 102 100 88
61 49 47 35 17 2 2 2
2
BssSI C'ACGA_G
Cuts at: 4166 5178 4166
Size: 1012 5094
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2232 794
Size: 1438 4668
BstBI TT'CG_AA
Cuts at: 4910 4910
Size: 6106
BstEII G'GTnAC_C
Cuts at: 1184 3208 1184
Size: 2024 4082
BstXI CCAn_nnnn'nTGG
Cuts at: 2845 2845
Size: 6106
BstYI r'GATC_y
Cuts at: 1972 3436 3803 4188 4205 5657 5743 5755 6101
Size: 1464 367 385 17 1452 86 12 346
Cuts at: 6101 1972
Size: 1977
Fragments arranged by size:
1977 1464 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2012 2155 2580 3003 3114 3375
Size: 552 939 8 143 425 423 111 261
Cuts at: 3375 513
Size: 3244
Fragments arranged by size:
3244 939 552 425 423 261 143 111
8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2131 487
Size: 683 56 905 4462
Fragments arranged by size:
4462 905 683 56
BtsI GCAGTG_nn'
Cuts at: 3622 4416 4436 3622
Size: 794 20 5292
Fragments arranged by size:
5292 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6106
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6071
DraI TTT'AAA
Cuts at: 3410 3410
Size: 6106
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5113 809
Size: 507 3797 1802
Fragments arranged by size:
3797 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2158 2397 2482
Size: 54 1425 50 275 18 328 239 85
Cuts at: 2482 2568 2577 3000 3033 3273 3315 3363 3553
Size: 86 9 423 33 240 42 48 190
Cuts at: 3553 4442 6071 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 423 328 275 240 239
190 86 85 54 50 48 43 42
33 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2397 2482 2577 3553 1537
Size: 275 585 85 95 976 4090
Fragments arranged by size:
4090 976 585 275 95 85
EarI CTCTTCn'nnn_
Cuts at: 1353 2546 3017 4036 1353
Size: 1193 471 1019 3423
Fragments arranged by size:
3423 1193 1019 471
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4693 5067 5213 387
Size: 1064 138 3104 374 146 1280
Fragments arranged by size:
3104 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3250 3250
Size: 6106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2372 2690 2867 3052 4169 4766
Size: 393 524 216 318 177 185 1117 597
Cuts at: 4766 5553 5821 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1117 787 597 524 393 318 268
216 185 177
EcoNI CCTnn'n_nnAGG
Cuts at: 3296 3296
Size: 6106
EcoO109I rG'GnC_Cy
Cuts at: 2046 2290 2459 3343 3344 2046
Size: 244 169 884 1 4808
Fragments arranged by size:
4808 884 244 169 1
EcoRI G'AATT_C
Cuts at: 3415 3793 3415
Size: 378 5728
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2758 2758
Size: 6106
FspI TGC'GCA
Cuts at: 4612 4612
Size: 6106
HaeII r_GCGC'y
Cuts at: 1678 2143 2258 2359 2494 2783 3011 3167 5253
Size: 465 115 101 135 289 228 156 2086
Cuts at: 5253 1678
Size: 2531
Fragments arranged by size:
2531 2086 465 289 228 156 135 115
101
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2109 2141 4747 4779 4821
Size: 32 135 32 537 32 2606 32 42
Cuts at: 4821 4853 1373
Size: 32 2626
Fragments arranged by size:
2626 2606 537 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3450 3696 669
Size: 988 281 1512 246 3079
Fragments arranged by size:
3079 1512 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6106
HpaI GTT'AAC
Cuts at: 3696 3696
Size: 6106
KasI G'GCGC_C
Cuts at: 2490 2490
Size: 6106
KpnI G_GTAC'C
Cuts at: 3431 3431
Size: 6106
MfeI C'AATT_G
Cuts at: 3705 3705
Size: 6106
MluI A'CGCG_T
Cuts at: 5982 5982
Size: 6106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5220 5404 1305
Size: 3915 184 2007
Fragments arranged by size:
3915 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3002 10
Size: 54 1425 343 1170 3114
Fragments arranged by size:
3114 1425 1170 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2072 2579 3104 4064 4423 4582 518
Size: 648 906 507 525 960 359 159 2042
Fragments arranged by size:
2042 960 906 648 525 507 359 159
MspA1I CmG'CkG
Cuts at: 1606 2157 2958 3183 3377 3522 4199 5347 5592
Size: 551 801 225 194 145 677 1148 245
Cuts at: 5592 1606
Size: 2120
Fragments arranged by size:
2120 1148 801 677 551 245 225 194
145
NaeI GCC'GGC
Cuts at: 2486 3288 2486
Size: 802 5304
NarI GG'CG_CC
Cuts at: 2491 2491
Size: 6106
NcoI C'CATG_G
Cuts at: 513 1065 2004 2012 3003 513
Size: 552 939 8 991 3616
Fragments arranged by size:
3616 991 939 552 8
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6106
NgoMIV G'CCGG_C
Cuts at: 2484 3286 2484
Size: 802 5304
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6106
NotI GC'GGCC_GC
Cuts at: 3553 3553
Size: 6106
NspI r_CATG'y
Cuts at: 1132 1229 3464 5009 1132
Size: 97 2235 1545 2229
Fragments arranged by size:
2235 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2455 1348
Size: 1107 4999
PciI A'CATG_T
Cuts at: 5005 5005
Size: 6106
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2923 3087 1108
Size: 1815 164 4127
Fragments arranged by size:
4127 1815 164
PfoI T'CCnGG_A
Cuts at: 2844 2844
Size: 6106
PmeI GTTT'AAAC
Cuts at: 3410 3410
Size: 6106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4175 4207 5688 5720 4175
Size: 32 1481 32 4561
Fragments arranged by size:
4561 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2046 2459 2046
Size: 413 5693
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6106
PsiI TTA'TAA
Cuts at: 3676 3676
Size: 6106
PspOMI G'GGCC_C
Cuts at: 2290 3343 2290
Size: 1053 5053
PstI C_TGCA'G
Cuts at: 830 2478 2724 3297 3458 830
Size: 1648 246 573 161 3478
Fragments arranged by size:
3478 1648 573 246 161
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6106
SacI G_AGCT'C
Cuts at: 3252 3252
Size: 6106
SacII CC_GC'GG
Cuts at: 2158 3378 2158
Size: 1220 4886
SalI G'TCGA_C
Cuts at: 1936 3448 1936
Size: 1512 4594
SanDI GG'GwC_CC
Cuts at: 2046 2046
Size: 6106
SapI GCTCTTCn'nnn_
Cuts at: 2546 3017 2546
Size: 471 5635
SbfI CC_TGCA'GG
Cuts at: 2724 3297 3458 2724
Size: 573 161 5372
Fragments arranged by size:
5372 573 161
ScaI AGT'ACT
Cuts at: 4354 6066 4354
Size: 1712 4394
SfcI C'TryA_G
Cuts at: 826 1045 2474 2720 3293 3454 4589 5270 5461
Size: 219 1429 246 573 161 1135 681 191
Cuts at: 5461 826
Size: 1471
Fragments arranged by size:
1471 1429 1135 681 573 246 219 191
161
SfiI GGCCn_nnn'nGGCC
Cuts at: 2576 2576
Size: 6106
SfoI GGC'GCC
Cuts at: 2492 2492
Size: 6106
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6106
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6106
SmaI CCC'GGG
Cuts at: 1872 2028 2829 3151 3433 1872
Size: 156 801 322 282 4545
Fragments arranged by size:
4545 801 322 282 156
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3251 4210 5111 5373 820
Size: 197 926 1308 959 901 262 1553
Fragments arranged by size:
1553 1308 959 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3897 493
Size: 3404 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6106
SphI G_CATG'C
Cuts at: 1132 3464 1132
Size: 2332 3774
SrfI GCCC'GGGC
Cuts at: 2829 3151 2829
Size: 322 5784
SspI AAT'ATT
Cuts at: 5 52 4030 5
Size: 47 3978 2081
Fragments arranged by size:
3978 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6106
StyI C'CwwG_G
Cuts at: 513 1065 2004 2012 3003 3545 513
Size: 552 939 8 991 542 3074
Fragments arranged by size:
3074 991 939 552 542 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3379 4133 4150 4303 4488 4933 325
Size: 1255 1799 754 17 153 185 445 1498
Fragments arranged by size:
1799 1498 1255 754 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4352 6064 96
Size: 274 80 33 51 1443 2375 1712 138
Fragments arranged by size:
2375 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2663 2695 3371 3403 6066 6098 2663
Size: 32 676 32 2663 32 2671
Fragments arranged by size:
2671 2663 676 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3131 1316
Size: 1815 4291
XbaI T'CTAG_A
Cuts at: 3442 3442
Size: 6106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3112 3112
Size: 6106
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6106
XmaI C'CCGG_G
Cuts at: 1870 2026 2827 3149 3431 1870
Size: 156 801 322 282 4545
Fragments arranged by size:
4545 801 322 282 156
XmnI GAAnn'nnTTC
Cuts at: 4235 4235
Size: 6106
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3914 276
Size: 53 83 186 936 2380 2468
Fragments arranged by size:
2468 2380 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoRV FseI FspAI NruI NsiI PacI PmlI
PspXI PsrI PvuII RsrII SexAI SwaI