Restriction map for
pFN21AE5021
(Circular) (Six-base) MAPSORT of: pFN21AE5021.seq Check: 8646 from: 1 to: 5995
pFN21AE5021 5995 bp
With 182 enzymes: SgfI *
December 8, 2014 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2905 2905
Size: 5995
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3805 278
Size: 53 83 186 936 2269 2468
Fragments arranged by size:
2468 2269 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3338 1937
Size: 1401 4594
Acc65I G'GTAC_C
Cuts at: 3316 3316
Size: 5995
AclI AA'CG_TT
Cuts at: 1563 4122 4495 1563
Size: 2559 373 3063
Fragments arranged by size:
3063 2559 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2224 2749 4058 5442 5710 1632
Size: 592 525 1309 1384 268 1917
Fragments arranged by size:
1917 1384 1309 592 525 268
AfeI AGC'GCT
Cuts at: 2245 2245
Size: 5995
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5798
AflIII A'CryG_T
Cuts at: 1203 4894 5871 1203
Size: 3691 977 1327
Fragments arranged by size:
3691 1327 977
AgeI A'CCGG_T
Cuts at: 4826 4826
Size: 5995
AhdI GACnn_n'nnGTC
Cuts at: 4724 4724
Size: 5995
AleI CACnn'nnGTG
Cuts at: 1166 2381 2714 1166
Size: 1215 333 4447
Fragments arranged by size:
4447 1215 333
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2585 1184
Size: 1401 4594
AlwNI CAG_nnn'CTG
Cuts at: 3738 5310 3738
Size: 1572 4423
ApaLI G'TGCA_C
Cuts at: 4052 5208 4052
Size: 1156 4839
ApoI r'AATT_y
Cuts at: 1493 3304 3530 3682 5791 1493
Size: 1811 226 152 2109 1697
Fragments arranged by size:
2109 1811 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2982 2982
Size: 5995
AseI AT'TA_AT
Cuts at: 160 1032 4549 160
Size: 872 3517 1606
Fragments arranged by size:
3517 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5995
AvaI C'yCGr_G
Cuts at: 1870 1943 2183 3097 3232 3320 1870
Size: 73 240 914 135 88 4545
Fragments arranged by size:
4545 914 240 135 88 73
BamHI G'GATC_C
Cuts at: 3325 3692 3325
Size: 367 5628
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2162 2222 2357 2375 3050
Size: 325 217 627 375 60 135 18 675
Cuts at: 3050 3316 4771 618
Size: 266 1455 1842
Fragments arranged by size:
1842 1455 675 627 375 325 266 217
135 60 18
BanII G_rGCy'C
Cuts at: 1391 2192 2829 3108 3314 1391
Size: 801 637 279 206 4072
Fragments arranged by size:
4072 801 637 279 206
BbeI G_GCGC'C
Cuts at: 2226 2361 2226
Size: 135 5860
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2164 928
Size: 971 265 4759
Fragments arranged by size:
4759 971 265
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4186 4220 1903
Size: 34 2249 34 3678
Fragments arranged by size:
3678 2249 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3887 5097 3887
Size: 1210 4785
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5995
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2306 2565 2625
Size: 107 122 71 631 605 634 259 60
Cuts at: 2625 3099 3225 4606 136
Size: 474 126 1381 1525
Fragments arranged by size:
1525 1381 634 631 605 474 259 126
122 107 71 60
BglII A'GATC_T
Cuts at: 5990 5990
Size: 5995
BlpI GC'TnA_GC
Cuts at: 2942 3412 2942
Size: 470 5525
Bme1580I G_kGCm'C
Cuts at: 1790 2378 4056 5212 1790
Size: 588 1678 1156 2573
Fragments arranged by size:
2573 1678 1156 588
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5995
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3726 4684 442
Size: 416 53 83 399 24 2309 958 1753
Fragments arranged by size:
2309 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5995
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3127 3127
Size: 5995
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2519 3274 4655 1239
Size: 1280 755 1381 2579
Fragments arranged by size:
2579 1381 1280 755
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4120 4985 5283 4120
Size: 865 298 4832
Fragments arranged by size:
4832 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3272 4658 882
Size: 617 1773 1386 2219
Fragments arranged by size:
2219 1773 1386 617
BsaAI yAC'GTr
Cuts at: 493 1204 2650 2734 3786 493
Size: 711 1446 84 1052 2702
Fragments arranged by size:
2702 1446 1052 711 84
BsaBI GATnn'nnATC
Cuts at: 5989 5989
Size: 5995
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2223 2316 2358 3078
Size: 53 83 186 936 690 93 42 720
Cuts at: 3078 3802 4184 4819 275
Size: 724 382 635 1451
Fragments arranged by size:
1451 936 724 720 690 635 382 186
93 83 53 42
BsaWI w'CCGG_w
Cuts at: 2814 4428 4826 5100 5247 5658 2814
Size: 1614 398 274 147 411 3151
Fragments arranged by size:
3151 1614 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2882 2912 1178
Size: 30 165 30 1479 30 4261
Fragments arranged by size:
4261 1479 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5995
BseYI C'CCAG_C
Cuts at: 2217 2343 2468 3250 5198 5694 2217
Size: 126 125 782 1948 496 2518
Fragments arranged by size:
2518 1948 782 496 126 125
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5422
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2116 3445 4206 5234 1540
Size: 275 186 115 1329 761 1028 2301
Fragments arranged by size:
2301 1329 1028 761 275 186 115
BsiHKAI G_wGCw'C
Cuts at: 2922 3108 4056 4141 5212 2922
Size: 186 948 85 1071 3705
Fragments arranged by size:
3705 1071 948 186 85
BsmI GAATG_Cn'
Cuts at: 3506 3599 3506
Size: 93 5902
BsmBI CGTCTCn'nnnn_
Cuts at: 3170 3170
Size: 5995
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2192 2378 2829 2922 3108 3314 4056
Size: 399 402 186 451 93 186 206 742
Cuts at: 4056 4141 5212 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 451 402 399 206 186
186 93 85
BspEI T'CCGG_A
Cuts at: 5658 5658
Size: 5995
BspHI T'CATG_A
Cuts at: 3258 3884 5614 3258
Size: 626 1730 3639
Fragments arranged by size:
3639 1730 626
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2905 3350 844
Size: 2061 445 3489
Fragments arranged by size:
3489 2061 445
BsrBI CCG'CTC
Cuts at: 1394 1823 3882 1394
Size: 429 2059 3507
Fragments arranged by size:
3507 2059 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3007 4490 4664 65
Size: 1183 1759 1483 174 1396
Fragments arranged by size:
1759 1483 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2111 2312 3267 4639 4826 1700
Size: 110 301 201 955 1372 187 2869
Fragments arranged by size:
2869 1372 955 301 201 187 110
BsrGI T'GTAC_A
Cuts at: 96 2706 96
Size: 2610 3385
BssHII G'CGCG_C
Cuts at: 1925 2213 2275 2533 2958 2960 2982 3246 1925
Size: 288 62 258 425 2 22 264 4674
Fragments arranged by size:
4674 425 288 264 258 62 22 2
BssSI C'ACGA_G
Cuts at: 4055 5067 4055
Size: 1012 4983
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3204 794
Size: 2410 3585
BstBI TT'CG_AA
Cuts at: 4799 4799
Size: 5995
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5995
BstYI r'GATC_y
Cuts at: 1972 3325 3692 4077 4094 5546 5632 5644 5990
Size: 1353 367 385 17 1452 86 12 346
Cuts at: 5990 1972
Size: 1977
Fragments arranged by size:
1977 1452 1353 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2379 2590 3060 513
Size: 552 1314 211 470 3448
Fragments arranged by size:
3448 1314 552 470 211
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2428 487
Size: 683 56 1202 4054
Fragments arranged by size:
4054 1202 683 56
BtsI GCAGTG_nn'
Cuts at: 3511 4305 4325 3511
Size: 794 20 5181
Fragments arranged by size:
5181 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5995
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5960
DraI TTT'AAA
Cuts at: 3299 3299
Size: 5995
DraIII CAC_nnn'GTG
Cuts at: 2257 2257
Size: 5995
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5002 809
Size: 507 3686 1802
Fragments arranged by size:
3686 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2113 2259 2307
Size: 54 1425 50 275 18 283 146 48
Cuts at: 2307 2700 3039 3180 3273 3442 4331 5960 8
Size: 393 339 141 93 169 889 1629 43
Fragments arranged by size:
1629 1425 889 393 339 283 275 169
146 141 93 54 50 48 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2113 3442 1537
Size: 275 301 1329 4090
Fragments arranged by size:
4090 1329 301 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2498 2855 2891 3925 1353
Size: 1145 357 36 1034 3423
Fragments arranged by size:
3423 1145 1034 357 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2216 2576 4582 4956 5102 387
Size: 1064 138 627 360 2006 374 146 1280
Fragments arranged by size:
2006 1280 1064 627 374 360 146 138
EcoICRI GAG'CTC
Cuts at: 3106 3106
Size: 5995
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2224 2519 2749 3274 4058 4655 5442
Size: 393 592 295 230 525 784 597 787
Cuts at: 5442 5710 1239
Size: 268 1524
Fragments arranged by size:
1524 787 784 597 592 525 393 295
268 230
EcoNI CCTnn'n_nnAGG
Cuts at: 2102 2102
Size: 5995
EcoO109I rG'GnC_Cy
Cuts at: 2785 2785
Size: 5995
EcoRI G'AATT_C
Cuts at: 3304 3682 3304
Size: 378 5617
FspI TGC'GCA
Cuts at: 2234 2250 2936 4501 2234
Size: 16 686 1565 3728
Fragments arranged by size:
3728 1565 686 16
HaeII r_GCGC'y
Cuts at: 1678 2138 2183 2226 2247 2361 2551 3155 3288
Size: 460 45 43 21 114 190 604 133
Cuts at: 3288 5142 1678
Size: 1854 2531
Fragments arranged by size:
2531 1854 604 460 190 133 114 45
43 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4636 4668 4710 4742 1373
Size: 32 135 32 3064 32 42 32 2626
Fragments arranged by size:
3064 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2388 3211 3339 3585 669
Size: 988 281 450 823 128 246 3079
Fragments arranged by size:
3079 988 823 450 281 246 128
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5995
HpaI GTT'AAC
Cuts at: 3585 3585
Size: 5995
KasI G'GCGC_C
Cuts at: 2222 2357 2222
Size: 135 5860
KpnI G_GTAC'C
Cuts at: 3320 3320
Size: 5995
MfeI C'AATT_G
Cuts at: 3594 3594
Size: 5995
MluI A'CGCG_T
Cuts at: 5871 5871
Size: 5995
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3153 3159 5109 5293 1305
Size: 1848 6 1950 184 2007
Fragments arranged by size:
2007 1950 1848 184 6
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4173
Fragments arranged by size:
4173 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2381 2714 3113 3953 4312 4471 518
Size: 648 1215 333 399 840 359 159 2042
Fragments arranged by size:
2042 1215 840 648 399 359 333 159
MspA1I CmG'CkG
Cuts at: 1606 2554 2565 2592 2952 3062 3169 3411 4088
Size: 948 11 27 360 110 107 242 677
Cuts at: 4088 5236 5481 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 948 677 360 245 242 110
107 27 11
NaeI GCC'GGC
Cuts at: 2314 2314
Size: 5995
NarI GG'CG_CC
Cuts at: 2223 2358 2223
Size: 135 5860
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5443
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5995
NgoMIV G'CCGG_C
Cuts at: 2312 2312
Size: 5995
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5995
NotI GC'GGCC_GC
Cuts at: 3442 3442
Size: 5995
NspI r_CATG'y
Cuts at: 1132 1229 3353 4898 1132
Size: 97 2124 1545 2229
Fragments arranged by size:
2229 2124 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5995
PciI A'CATG_T
Cuts at: 4894 4894
Size: 5995
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5995
PfoI T'CCnGG_A
Cuts at: 2496 2496
Size: 5995
PmeI GTTT'AAAC
Cuts at: 3299 3299
Size: 5995
PmlI CAC'GTG
Cuts at: 2650 2650
Size: 5995
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3215 3247 4064 4096 5577 5609 3215
Size: 32 817 32 1481 32 3601
Fragments arranged by size:
3601 1481 817 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2785 2785
Size: 5995
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5995
PsiI TTA'TAA
Cuts at: 3565 3565
Size: 5995
PstI C_TGCA'G
Cuts at: 830 3162 3347 830
Size: 2332 185 3478
Fragments arranged by size:
3478 2332 185
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5995
PvuII CAG'CTG
Cuts at: 2554 3169 2554
Size: 615 5380
SacI G_AGCT'C
Cuts at: 3108 3108
Size: 5995
SacII CC_GC'GG
Cuts at: 2593 3063 2593
Size: 470 5525
SalI G'TCGA_C
Cuts at: 1936 3337 1936
Size: 1401 4594
SapI GCTCTTCn'nnn_
Cuts at: 2891 2891
Size: 5995
SbfI CC_TGCA'GG
Cuts at: 3347 3347
Size: 5995
ScaI AGT'ACT
Cuts at: 4243 5955 4243
Size: 1712 4283
SexAI A'CCwGG_T
Cuts at: 2151 2787 2151
Size: 636 5359
SfcI C'TryA_G
Cuts at: 826 1045 3158 3343 4478 5159 5350 826
Size: 219 2113 185 1135 681 191 1471
Fragments arranged by size:
2113 1471 1135 681 219 191 185
SfiI GGCCn_nnn'nGGCC
Cuts at: 2306 2306
Size: 5995
SfoI GGC'GCC
Cuts at: 2224 2359 2224
Size: 135 5860
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5995
SgrAI Cr'CCGG_yG
Cuts at: 1700 2312 1700
Size: 612 5383
SmaI CCC'GGG
Cuts at: 1872 3322 1872
Size: 1450 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2183 4099 5000 5262 820
Size: 197 926 240 1916 901 262 1553
Fragments arranged by size:
1916 1553 926 901 262 240 197
SnaBI TAC'GTA
Cuts at: 493 3786 493
Size: 3293 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5995
SphI G_CATG'C
Cuts at: 1132 3353 1132
Size: 2221 3774
SspI AAT'ATT
Cuts at: 5 52 3919 5
Size: 47 3867 2081
Fragments arranged by size:
3867 2081 47
StuI AGG'CCT
Cuts at: 1517 2435 1517
Size: 918 5077
StyI C'CwwG_G
Cuts at: 513 1065 3434 513
Size: 552 2369 3074
Fragments arranged by size:
3074 2369 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4022 4039 4192 4377 4822 325
Size: 1255 2442 17 153 185 445 1498
Fragments arranged by size:
2442 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2706 4241 5953
Size: 274 80 33 51 1443 729 1535 1712
Cuts at: 5953 96
Size: 138
Fragments arranged by size:
1712 1535 1443 729 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5955 5987 5955
Size: 32 5963
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5995
XbaI T'CTAG_A
Cuts at: 3331 3331
Size: 5995
XhoI C'TCGA_G
Cuts at: 1943 2183 1943
Size: 240 5755
XmaI C'CCGG_G
Cuts at: 1870 3320 1870
Size: 1450 4545
XmnI GAAnn'nnTTC
Cuts at: 2435 3227 4124 2435
Size: 792 897 4306
Fragments arranged by size:
4306 897 792
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3803 276
Size: 53 83 186 936 2269 2468
Fragments arranged by size:
2468 2269 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI ApaI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI
BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI
NsiI PacI PspOMI PspXI PsrI RsrII SanDI SrfI
SwaI XcmI