Restriction map for
pFN21AE5019
(Circular) (Six-base) MAPSORT of: pFN21AE5019.seq Check: 2750 from: 1 to: 5755
pFN21AE5019 5755 bp
With 182 enzymes: SgfI *
December 8, 2014 13:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3565 278
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2766 3098 1937
Size: 829 332 4594
Fragments arranged by size:
4594 829 332
Acc65I G'GTAC_C
Cuts at: 2814 3076 2814
Size: 262 5493
AclI AA'CG_TT
Cuts at: 1563 3882 4255 1563
Size: 2319 373 3063
Fragments arranged by size:
3063 2319 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2295 2884 3818 5202 5470 1632
Size: 663 589 934 1384 268 1917
Fragments arranged by size:
1917 1384 934 663 589 268
AfeI AGC'GCT
Cuts at: 2217 2217
Size: 5755
AflII C'TTAA_G
Cuts at: 820 1017 2666 820
Size: 197 1649 3909
Fragments arranged by size:
3909 1649 197
AflIII A'CryG_T
Cuts at: 1203 4654 5631 1203
Size: 3451 977 1327
Fragments arranged by size:
3451 1327 977
AgeI A'CCGG_T
Cuts at: 4586 4586
Size: 5755
AhdI GACnn_n'nnGTC
Cuts at: 2181 4484 2181
Size: 2303 3452
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5755
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5755
AlwNI CAG_nnn'CTG
Cuts at: 2075 2609 2779 3498 5070 2075
Size: 534 170 719 1572 2760
Fragments arranged by size:
2760 1572 719 534 170
ApaLI G'TGCA_C
Cuts at: 3812 4968 3812
Size: 1156 4599
ApoI r'AATT_y
Cuts at: 1493 3064 3290 3442 5551 1493
Size: 1571 226 152 2109 1697
Fragments arranged by size:
2109 1697 1571 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4309 160
Size: 872 3277 1606
Fragments arranged by size:
3277 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
AvaI C'yCGr_G
Cuts at: 1870 1943 2807 3080 1870
Size: 73 864 273 4545
Fragments arranged by size:
4545 864 273 73
BamHI G'GATC_C
Cuts at: 3085 3452 3085
Size: 367 5388
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2107 2454 2814 3076 4531
Size: 325 217 627 320 347 360 262 1455
Cuts at: 4531 618
Size: 1842
Fragments arranged by size:
1842 1455 627 360 347 325 320 262
217
BanII G_rGCy'C
Cuts at: 1391 2099 2128 2230 3074 1391
Size: 708 29 102 844 4072
Fragments arranged by size:
4072 844 708 102 29
BbeI G_GCGC'C
Cuts at: 2458 2458
Size: 5755
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2890 928
Size: 971 991 3793
Fragments arranged by size:
3793 991 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3946 3980 1903
Size: 34 50 34 1925 34 3678
Fragments arranged by size:
3678 1925 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2790 3647 4857 2790
Size: 857 1210 3688
Fragments arranged by size:
3688 1210 857
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5755
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4366 136
Size: 107 122 71 631 605 2694 1525
Fragments arranged by size:
2694 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5750 5750
Size: 5755
BlpI GC'TnA_GC
Cuts at: 2481 3172 2481
Size: 691 5064
Bme1580I G_kGCm'C
Cuts at: 1790 2050 2846 3816 4972 1790
Size: 260 796 970 1156 2573
Fragments arranged by size:
2573 1156 970 796 260
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5755
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2789 3486 4444
Size: 416 53 83 399 24 1372 697 958
Cuts at: 4444 442
Size: 1753
Fragments arranged by size:
1753 1372 958 697 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2144 1056
Size: 1088 4667
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2274 2274
Size: 5755
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2244 2709 4415 1239
Size: 917 88 465 1706 2579
Fragments arranged by size:
2579 1706 917 465 88
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2443 2557 3880 4745 5043 2443
Size: 114 1323 865 298 3155
Fragments arranged by size:
3155 1323 865 298 114
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2748 4418 882
Size: 617 1249 1670 2219
Fragments arranged by size:
2219 1670 1249 617
BsaAI yAC'GTr
Cuts at: 493 1204 2392 2764 3546 493
Size: 711 1188 372 782 2702
Fragments arranged by size:
2702 1188 782 711 372
BsaBI GATnn'nnATC
Cuts at: 5749 5749
Size: 5755
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2455 3562 3944 4579
Size: 53 83 186 936 922 1107 382 635
Cuts at: 4579 275
Size: 1451
Fragments arranged by size:
1451 1107 936 922 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2232 4188 4586 4860 5007 5418 2232
Size: 1956 398 274 147 411 2569
Fragments arranged by size:
2569 1956 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2185 2215 1178
Size: 30 165 30 782 30 4718
Fragments arranged by size:
4718 782 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2288 2340 2464 2722 2897 2969 1186
Size: 1102 52 124 258 175 72 3972
Fragments arranged by size:
3972 1102 258 175 124 72 52
BseYI C'CCAG_C
Cuts at: 4958 5454 4958
Size: 496 5259
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2372 1163
Size: 573 636 4546
Fragments arranged by size:
4546 636 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3205 3966 4994 1540
Size: 275 186 1204 761 1028 2301
Fragments arranged by size:
2301 1204 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2062 2128 2397 2487 3816 3901 4972 2062
Size: 66 269 90 1329 85 1071 2845
Fragments arranged by size:
2845 1329 1071 269 90 85 66
BsmI GAATG_Cn'
Cuts at: 3266 3359 3266
Size: 93 5662
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2050 2062 2099 2128 2230 2397 2487
Size: 399 260 12 37 29 102 167 90
Cuts at: 2487 2846 3074 3816 3901 4972 1391
Size: 359 228 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 359 260 228 167
102 90 85 37 29 12
BspEI T'CCGG_A
Cuts at: 2232 5418 2232
Size: 3186 2569
BspHI T'CATG_A
Cuts at: 3644 5374 3644
Size: 1730 4025
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3110 844
Size: 2266 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3642 1394
Size: 429 1819 3507
Fragments arranged by size:
3507 1819 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2871 3007 4250 4424 65
Size: 1183 1623 136 1243 174 1396
Fragments arranged by size:
1623 1396 1243 1183 174 136
BsrFI r'CCGG_y
Cuts at: 1700 1810 4399 4586 1700
Size: 110 2589 187 2869
Fragments arranged by size:
2869 2589 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5755
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5755
BssSI C'ACGA_G
Cuts at: 3815 4827 3815
Size: 1012 4743
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2418 794
Size: 1624 4131
BstBI TT'CG_AA
Cuts at: 4559 4559
Size: 5755
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5755
BstXI CCAn_nnnn'nTGG
Cuts at: 2858 2858
Size: 5755
BstYI r'GATC_y
Cuts at: 1972 2427 2670 3085 3452 3837 3854 5306 5392
Size: 455 243 415 367 385 17 1452 86
Cuts at: 5392 5404 5750 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 455 415 385 367 346 243
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5203
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5016
Fragments arranged by size:
5016 683 56
BtsI GCAGTG_nn'
Cuts at: 2839 2846 3271 4065 4085 2839
Size: 7 425 794 20 4509
Fragments arranged by size:
4509 794 425 20 7
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5755
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5720
DraI TTT'AAA
Cuts at: 3059 3059
Size: 5755
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4762 809
Size: 507 3446 1802
Fragments arranged by size:
3446 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3202 4091 5720
Size: 54 1425 50 275 18 1372 889 1629
Cuts at: 5720 8
Size: 43
Fragments arranged by size:
1629 1425 1372 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3202 1537
Size: 275 1390 4090
Fragments arranged by size:
4090 1390 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3685 1353
Size: 2332 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4342 4716 4862 387
Size: 1064 138 2753 374 146 1280
Fragments arranged by size:
2753 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2126 2126
Size: 5755
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2244 2295 2709 2884 3818 4415
Size: 393 524 88 51 414 175 934 597
Cuts at: 4415 5202 5470 1239
Size: 787 268 1524
Fragments arranged by size:
1524 934 787 597 524 414 393 268
175 88 51
EcoNI CCTnn'n_nnAGG
Cuts at: 2691 2691
Size: 5755
EcoO109I rG'GnC_Cy
Cuts at: 2463 2712 2967 2463
Size: 249 255 5251
Fragments arranged by size:
5251 255 249
EcoRI G'AATT_C
Cuts at: 3064 3442 3064
Size: 378 5377
FspI TGC'GCA
Cuts at: 4261 4261
Size: 5755
HaeII r_GCGC'y
Cuts at: 1678 2219 2458 4902 1678
Size: 541 239 2444 2531
Fragments arranged by size:
2531 2444 541 239
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2167 2199 4396 4428 4470
Size: 32 135 32 595 32 2197 32 42
Cuts at: 4470 4502 1373
Size: 32 2626
Fragments arranged by size:
2626 2197 595 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3099 3345 669
Size: 988 281 1161 246 3079
Fragments arranged by size:
3079 1161 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5755
HpaI GTT'AAC
Cuts at: 3345 3345
Size: 5755
KasI G'GCGC_C
Cuts at: 2454 2454
Size: 5755
KpnI G_GTAC'C
Cuts at: 2818 3080 2818
Size: 262 5493
MfeI C'AATT_G
Cuts at: 3354 3354
Size: 5755
MluI A'CGCG_T
Cuts at: 5631 5631
Size: 5755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4869 5053 1305
Size: 3564 184 2007
Fragments arranged by size:
3564 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3933
Fragments arranged by size:
3933 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3713 4072 4231 518
Size: 648 2547 359 159 2042
Fragments arranged by size:
2547 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2821 3171 3848 4996 5241 1606
Size: 1215 350 677 1148 245 2120
Fragments arranged by size:
2120 1215 1148 677 350 245
NarI GG'CG_CC
Cuts at: 2455 2455
Size: 5755
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5203
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5755
NheI G'CTAG_C
Cuts at: 1052 2140 1052
Size: 1088 4667
NotI GC'GGCC_GC
Cuts at: 3202 3202
Size: 5755
NspI r_CATG'y
Cuts at: 1132 1229 3113 4658 1132
Size: 97 1884 1545 2229
Fragments arranged by size:
2229 1884 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5755
PciI A'CATG_T
Cuts at: 4654 4654
Size: 5755
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2267 2779 1108
Size: 1159 512 4084
Fragments arranged by size:
4084 1159 512
PfoI T'CCnGG_A
Cuts at: 2157 2772 2157
Size: 615 5140
PmeI GTTT'AAAC
Cuts at: 3059 3059
Size: 5755
PmlI CAC'GTG
Cuts at: 2392 2392
Size: 5755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3824 3856 5337 5369 3824
Size: 32 1481 32 4210
Fragments arranged by size:
4210 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2463 2712 2463
Size: 249 5506
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5755
PsiI TTA'TAA
Cuts at: 3325 3325
Size: 5755
PstI C_TGCA'G
Cuts at: 830 2582 2805 2834 3053 3107 830
Size: 1752 223 29 219 54 3478
Fragments arranged by size:
3478 1752 223 219 54 29
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5755
PvuII CAG'CTG
Cuts at: 2821 2821
Size: 5755
SacI G_AGCT'C
Cuts at: 2128 2128
Size: 5755
SalI G'TCGA_C
Cuts at: 1936 3097 1936
Size: 1161 4594
SanDI GG'GwC_CC
Cuts at: 2463 2463
Size: 5755
SbfI CC_TGCA'GG
Cuts at: 3107 3107
Size: 5755
ScaI AGT'ACT
Cuts at: 4003 5715 4003
Size: 1712 4043
SfcI C'TryA_G
Cuts at: 826 1045 2240 2317 2578 2801 2830 3049 3103
Size: 219 1195 77 261 223 29 219 54
Cuts at: 3103 4238 4919 5110 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1195 1135 681 261 223 219 219
191 77 54 29
SfoI GGC'GCC
Cuts at: 2456 2456
Size: 5755
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5755
SmaI CCC'GGG
Cuts at: 1872 3082 1872
Size: 1210 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2422 2536 2666 3859 4760 5022
Size: 197 926 479 114 130 1193 901 262
Cuts at: 5022 820
Size: 1553
Fragments arranged by size:
1553 1193 926 901 479 262 197 130
114
SnaBI TAC'GTA
Cuts at: 493 2764 3546 493
Size: 2271 782 2702
Fragments arranged by size:
2702 2271 782
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5755
SphI G_CATG'C
Cuts at: 1132 3113 1132
Size: 1981 3774
SspI AAT'ATT
Cuts at: 5 52 3679 5
Size: 47 3627 2081
Fragments arranged by size:
3627 2081 47
StuI AGG'CCT
Cuts at: 1517 3041 1517
Size: 1524 4231
StyI C'CwwG_G
Cuts at: 513 1065 2444 2458 2924 3194 513
Size: 552 1379 14 466 270 3074
Fragments arranged by size:
3074 1379 552 466 270 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2343 3782 3799 3952 4137 4582 325
Size: 1255 763 1439 17 153 185 445 1498
Fragments arranged by size:
1498 1439 1255 763 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4001 5713 96
Size: 274 80 33 51 1443 2024 1712 138
Fragments arranged by size:
2024 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5715 5747 5715
Size: 32 5723
Tth111I GACn'n_nGTC
Cuts at: 1316 2071 1316
Size: 755 5000
XbaI T'CTAG_A
Cuts at: 3091 3091
Size: 5755
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5755
XmaI C'CCGG_G
Cuts at: 1870 3080 1870
Size: 1210 4545
XmnI GAAnn'nnTTC
Cuts at: 3884 3884
Size: 5755
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3563 276
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI
PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI
SwaI XcmI