Restriction map for
pFN21AE5012
(Circular) (Six-base) MAPSORT of: pFN21AE5012.seq Check: 4547 from: 1 to: 7708
pFN21AE5012 7708 bp
With 182 enzymes: SgfI *
December 8, 2014 13:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5518 278
Size: 53 83 186 936 3982 2468
Fragments arranged by size:
3982 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5051 1937
Size: 3114 4594
Acc65I G'GTAC_C
Cuts at: 5029 5029
Size: 7708
AclI AA'CG_TT
Cuts at: 1563 3379 5835 6208 1563
Size: 1816 2456 373 3063
Fragments arranged by size:
3063 2456 1816 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2610 2775 3116 3703 3724 3748 3772 3820
Size: 978 165 341 587 21 24 24 48
Cuts at: 3820 3916 3940 4049 4084 4132 4276 4420 4468
Size: 96 24 109 35 48 144 144 48
Cuts at: 4468 4516 5771 7155 7423 1632
Size: 48 1255 1384 268 1917
Fragments arranged by size:
1917 1384 1255 978 587 341 268 165
144 144 109 96 48 48 48 48
35 24 24 24 21
AflII C'TTAA_G
Cuts at: 820 1017 3979 820
Size: 197 2962 4549
Fragments arranged by size:
4549 2962 197
AflIII A'CryG_T
Cuts at: 1203 2518 2831 3157 6607 7584 1203
Size: 1315 313 326 3450 977 1327
Fragments arranged by size:
3450 1327 1315 977 326 313
AgeI A'CCGG_T
Cuts at: 6539 6539
Size: 7708
AhdI GACnn_n'nnGTC
Cuts at: 2320 6437 2320
Size: 4117 3591
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7708
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7708
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2844 2876 3686 3718 2844
Size: 32 810 32 6834
Fragments arranged by size:
6834 810 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2297 3202 5451 7023 2297
Size: 905 2249 1572 2982
Fragments arranged by size:
2982 2249 1572 905
ApaI G_GGCC'C
Cuts at: 4445 4983 4445
Size: 538 7170
ApaLI G'TGCA_C
Cuts at: 5765 6921 5765
Size: 1156 6552
ApoI r'AATT_y
Cuts at: 1493 2603 2908 3220 3344 3460 4663 4824 5017
Size: 1110 305 312 124 116 1203 161 193
Cuts at: 5017 5243 5395 7504 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1203 1110 312 305 226 193
161 152 124 116
AseI AT'TA_AT
Cuts at: 160 1032 2382 2781 6262 160
Size: 872 1350 399 3481 1606
Fragments arranged by size:
3481 1606 1350 872 399
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7708
AvaI C'yCGr_G
Cuts at: 1870 1943 3944 4088 4398 5033 1870
Size: 73 2001 144 310 635 4545
Fragments arranged by size:
4545 2001 635 310 144 73
AvrII C'CTAG_G
Cuts at: 3400 3749 3845 3400
Size: 349 96 7263
Fragments arranged by size:
7263 349 96
BamHI G'GATC_C
Cuts at: 5038 5405 5038
Size: 367 7341
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3660 3792 3816 3840 4320
Size: 325 217 627 1873 132 24 24 480
Cuts at: 4320 4416 4464 5029 6484 618
Size: 96 48 565 1455 1842
Fragments arranged by size:
1873 1842 1455 627 565 480 325 217
132 96 48 24 24
BanII G_rGCy'C
Cuts at: 1391 2217 4445 4493 4628 4983 5027 1391
Size: 826 2228 48 135 355 44 4072
Fragments arranged by size:
4072 2228 826 355 135 48 44
BbeI G_GCGC'C
Cuts at: 3796 3820 4324 3796
Size: 24 504 7180
Fragments arranged by size:
7180 504 24
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2252 3678 3765 4437 4461 4557 928
Size: 971 353 1426 87 672 24 96 4079
Fragments arranged by size:
4079 1426 971 672 353 96 87 24
BbvCI CC'TCA_GC
Cuts at: 3282 3282
Size: 7708
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4181 4215 5899 5933 1903
Size: 34 2244 34 1684 34 3678
Fragments arranged by size:
3678 2244 1684 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5600 6810 5600
Size: 1210 6498
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7708
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3658 4978 6319
Size: 107 122 71 631 605 1986 1320 1341
Cuts at: 6319 136
Size: 1525
Fragments arranged by size:
1986 1525 1341 1320 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2750 4957 7703 2750
Size: 2207 2746 2755
Fragments arranged by size:
2755 2746 2207
BlpI GC'TnA_GC
Cuts at: 5125 5125
Size: 7708
Bme1580I G_kGCm'C
Cuts at: 1790 4445 4983 5769 6925 1790
Size: 2655 538 786 1156 2573
Fragments arranged by size:
2655 2573 1156 786 538
BmgBI CAC'GTC
Cuts at: 1318 2168 1318
Size: 850 6858
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2036 5439 6397
Size: 416 53 83 399 24 619 3403 958
Cuts at: 6397 442
Size: 1753
Fragments arranged by size:
3403 1753 958 619 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7708
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4200 4248 4200
Size: 48 7660
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3651 3880 3904 3917 4048 4218 6368 1239
Size: 2412 229 24 13 131 170 2150 2579
Fragments arranged by size:
2579 2412 2150 229 170 131 24 13
Bpu10I CC'TnA_GC
Cuts at: 3282 3282
Size: 7708
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3162 3625 3893 5833 6698 6996 3162
Size: 463 268 1940 865 298 3874
Fragments arranged by size:
3874 1940 865 463 298 268
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2811 3921 4219 4339 6371 882
Size: 617 1312 1110 298 120 2032 2219
Fragments arranged by size:
2219 2032 1312 1110 617 298 120
BsaAI yAC'GTr
Cuts at: 493 1204 2519 3509 5499 493
Size: 711 1315 990 1990 2702
Fragments arranged by size:
2702 1990 1315 990 711
BsaBI GATnn'nnATC
Cuts at: 7702 7702
Size: 7708
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2287 3793 3817 4321
Size: 53 83 186 936 754 1506 24 504
Cuts at: 4321 5515 5897 6532 275
Size: 1194 382 635 1451
Fragments arranged by size:
1506 1451 1194 936 754 635 504 382
186 83 53 24
BsaWI w'CCGG_w
Cuts at: 3823 4173 4221 4341 4567 6141 6539 6813 6960
Size: 350 48 120 226 1574 398 274 147
Cuts at: 6960 7371 3823
Size: 411 4160
Fragments arranged by size:
4160 1574 411 398 350 274 226 147
120 48
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7483
Fragments arranged by size:
7483 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2112 3676 3794 3866 3877 3890 3914 3962
Size: 926 1564 118 72 11 13 24 48
Cuts at: 3962 3986 4034 4058 4130 4154 4586 1186
Size: 24 48 24 72 24 432 4308
Fragments arranged by size:
4308 1564 926 432 118 72 72 48
48 24 24 24 24 13 11
BseYI C'CCAG_C
Cuts at: 2393 6911 7407 2393
Size: 4518 496 2694
Fragments arranged by size:
4518 2694 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3272 1163
Size: 573 1536 5599
Fragments arranged by size:
5599 1536 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4373 5158 5919 6947 1540
Size: 275 186 2372 785 761 1028 2301
Fragments arranged by size:
2372 2301 1028 785 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2217 4266 4290 4338 5769 5854 6925 2217
Size: 2049 24 48 1431 85 1071 3000
Fragments arranged by size:
3000 2049 1431 1071 85 48 24
BsmI GAATG_Cn'
Cuts at: 4129 5219 5312 4129
Size: 1090 93 6525
Fragments arranged by size:
6525 1090 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4024 4024
Size: 7708
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2217 4266 4290 4338 4445 4493 4628
Size: 399 427 2049 24 48 107 48 135
Cuts at: 4628 4983 5027 5769 5854 6925 1391
Size: 355 44 742 85 1071 2174
Fragments arranged by size:
2174 2049 1071 742 427 399 355 135
107 85 48 48 44 24
BspEI T'CCGG_A
Cuts at: 3823 4173 4221 4341 4567 7371 3823
Size: 350 48 120 226 2804 4160
Fragments arranged by size:
4160 2804 350 226 120 48
BspHI T'CATG_A
Cuts at: 2569 5597 7327 2569
Size: 3028 1730 2950
Fragments arranged by size:
3028 2950 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5063 844
Size: 4219 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3023 5595 1394
Size: 429 1200 2572 3507
Fragments arranged by size:
3507 2572 1200 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3102 6203 6377 65
Size: 1183 1854 3101 174 1396
Fragments arranged by size:
3101 1854 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6352 6539 1700
Size: 110 4542 187 2869
Fragments arranged by size:
4542 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7708
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7708
BssSI C'ACGA_G
Cuts at: 5768 6780 5768
Size: 1012 6696
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2168 794
Size: 1374 6334
BstBI TT'CG_AA
Cuts at: 2797 6512 2797
Size: 3715 3993
BstEII G'GTnAC_C
Cuts at: 1184 3518 1184
Size: 2334 5374
BstYI r'GATC_y
Cuts at: 1972 2294 2750 4957 5038 5405 5790 5807 7259
Size: 322 456 2207 81 367 385 17 1452
Cuts at: 7259 7345 7357 7703 1972
Size: 86 12 346 1977
Fragments arranged by size:
2207 1977 1452 456 385 367 346 322
86 81 17 12
Bsu36I CC'TnA_GG
Cuts at: 3969 4017 4137 4185 4377 4556 4725 3969
Size: 48 120 48 192 179 169 6952
Fragments arranged by size:
6952 192 179 169 120 48 48
BtgI C'CryG_G
Cuts at: 513 1065 2004 3921 4840 513
Size: 552 939 1917 919 3381
Fragments arranged by size:
3381 1917 939 919 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6969
Fragments arranged by size:
6969 683 56
BtsI GCAGTG_nn'
Cuts at: 5224 6018 6038 5224
Size: 794 20 6894
Fragments arranged by size:
6894 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7708
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7673
DraI TTT'AAA
Cuts at: 3349 4807 5012 3349
Size: 1458 205 6045
Fragments arranged by size:
6045 1458 205
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6715 809
Size: 507 5399 1802
Fragments arranged by size:
5399 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2575 3193 3890
Size: 54 1425 50 275 18 745 618 697
Cuts at: 3890 4010 4202 4274 4298 4370 5155 6044 7673
Size: 120 192 72 24 72 785 889 1629
Cuts at: 7673 8
Size: 43
Fragments arranged by size:
1629 1425 889 785 745 697 618 275
192 120 72 72 54 50 43 24
18
EagI C'GGCC_G
Cuts at: 1537 1812 4370 5155 1537
Size: 275 2558 785 4090
Fragments arranged by size:
4090 2558 785 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2275 2380 3116 4068 5638 1353
Size: 922 105 736 952 1570 3423
Fragments arranged by size:
3423 1570 952 922 736 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6295 6669 6815 387
Size: 1064 138 4706 374 146 1280
Fragments arranged by size:
4706 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2215 2215
Size: 7708
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2610 2775 3116 3651 3703 3724 3748
Size: 393 978 165 341 535 52 21 24
Cuts at: 3748 3772 3820 3880 3904 3916 3917 3940 4048
Size: 24 48 60 24 12 1 23 108
Cuts at: 4048 4049 4084 4132 4218 4276 4420 4468 4516
Size: 1 35 48 86 58 144 48 48
Cuts at: 4516 5771 6368 7155 7423 1239
Size: 1255 597 787 268 1524
Fragments arranged by size:
1524 1255 978 787 597 535 393 341
268 165 144 108 86 60 58 52
48 48 48 48 35 24 24 24
23 21 12 1 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2094 3875 2094
Size: 1781 5927
EcoO109I rG'GnC_Cy
Cuts at: 2048 3867 4441 4442 4576 4980 2048
Size: 1819 574 1 134 404 4776
Fragments arranged by size:
4776 1819 574 404 134 1
EcoRI G'AATT_C
Cuts at: 5017 5395 5017
Size: 378 7330
FspI TGC'GCA
Cuts at: 6214 6214
Size: 7708
HaeII r_GCGC'y
Cuts at: 1678 3796 3820 4324 4396 6855 1678
Size: 2118 24 504 72 2459 2531
Fragments arranged by size:
2531 2459 2118 504 72 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2178 2210 2306 2338 4780
Size: 32 135 32 606 32 96 32 2442
Cuts at: 4780 4812 6349 6381 6423 6455 1373
Size: 32 1537 32 42 32 2626
Fragments arranged by size:
2626 2442 1537 606 135 96 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2314 4998 5052 5298 669
Size: 988 281 376 2684 54 246 3079
Fragments arranged by size:
3079 2684 988 376 281 246 54
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7708
HpaI GTT'AAC
Cuts at: 4998 5298 4998
Size: 300 7408
KasI G'GCGC_C
Cuts at: 3792 3816 4320 3792
Size: 24 504 7180
Fragments arranged by size:
7180 504 24
KpnI G_GTAC'C
Cuts at: 5033 5033
Size: 7708
MfeI C'AATT_G
Cuts at: 5307 5307
Size: 7708
MluI A'CGCG_T
Cuts at: 7584 7584
Size: 7708
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3703 6822 7006 1305
Size: 2398 3119 184 2007
Fragments arranged by size:
3119 2398 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3195 10
Size: 54 1425 343 1363 4523
Fragments arranged by size:
4523 1425 1363 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2406 2571 5666 6025 6184 518
Size: 648 1240 165 3095 359 159 2042
Fragments arranged by size:
3095 2042 1240 648 359 165 159
MspA1I CmG'CkG
Cuts at: 1606 5124 5801 6949 7194 1606
Size: 3518 677 1148 245 2120
Fragments arranged by size:
3518 2120 1148 677 245
NarI GG'CG_CC
Cuts at: 3793 3817 4321 3793
Size: 24 504 7180
Fragments arranged by size:
7180 504 24
NcoI C'CATG_G
Cuts at: 513 1065 2004 4840 513
Size: 552 939 2836 3381
Fragments arranged by size:
3381 2836 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7708
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7708
NotI GC'GGCC_GC
Cuts at: 4370 5155 4370
Size: 785 6923
NspI r_CATG'y
Cuts at: 1132 1229 3161 5066 6611 1132
Size: 97 1932 1905 1545 2229
Fragments arranged by size:
2229 1932 1905 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7708
PciI A'CATG_T
Cuts at: 3157 6607 3157
Size: 3450 4258
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2297 4682 1108
Size: 1189 2385 4134
Fragments arranged by size:
4134 2385 1189
PfoI T'CCnGG_A
Cuts at: 2290 2290
Size: 7708
PmeI GTTT'AAAC
Cuts at: 5012 5012
Size: 7708
PmlI CAC'GTG
Cuts at: 3509 3509
Size: 7708
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2821 2853 5777 5809 7290 7322 2821
Size: 32 2924 32 1481 32 3207
Fragments arranged by size:
3207 2924 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4576 4576
Size: 7708
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7708
PsiI TTA'TAA
Cuts at: 5278 5278
Size: 7708
PspOMI G'GGCC_C
Cuts at: 4441 4979 4441
Size: 538 7170
PstI C_TGCA'G
Cuts at: 830 5060 830
Size: 4230 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7708
SacI G_AGCT'C
Cuts at: 2217 2217
Size: 7708
SalI G'TCGA_C
Cuts at: 1936 5050 1936
Size: 3114 4594
SapI GCTCTTCn'nnn_
Cuts at: 2380 2380
Size: 7708
SbfI CC_TGCA'GG
Cuts at: 5060 5060
Size: 7708
ScaI AGT'ACT
Cuts at: 5956 7668 5956
Size: 1712 5996
SfcI C'TryA_G
Cuts at: 826 1045 2010 2208 2250 4899 5056 6191 6872
Size: 219 965 198 42 2649 157 1135 681
Cuts at: 6872 7063 826
Size: 191 1471
Fragments arranged by size:
2649 1471 1135 965 681 219 198 191
157 42
SfiI GGCCn_nnn'nGGCC
Cuts at: 4978 4978
Size: 7708
SfoI GGC'GCC
Cuts at: 3794 3818 4322 3794
Size: 24 504 7180
Fragments arranged by size:
7180 504 24
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7708
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7708
SmaI CCC'GGG
Cuts at: 1872 5035 1872
Size: 3163 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3177 3604 3872 3979 5812 6713
Size: 197 926 1234 427 268 107 1833 901
Cuts at: 6713 6975 820
Size: 262 1553
Fragments arranged by size:
1833 1553 1234 926 901 427 268 262
197 107
SnaBI TAC'GTA
Cuts at: 493 5499 493
Size: 5006 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7708
SphI G_CATG'C
Cuts at: 1132 5066 1132
Size: 3934 3774
SspI AAT'ATT
Cuts at: 5 52 5632 5
Size: 47 5580 2081
Fragments arranged by size:
5580 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7708
StyI C'CwwG_G
Cuts at: 513 1065 2004 3400 3749 3797 3845 4840 5147
Size: 552 939 1396 349 48 48 995 307
Cuts at: 5147 513
Size: 3074
Fragments arranged by size:
3074 1396 995 939 552 349 307 48
48
SwaI ATTT'AAAT
Cuts at: 3349 3349
Size: 7708
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4434 5735 5752 5905 6090 6535 325
Size: 1255 2854 1301 17 153 185 445 1498
Fragments arranged by size:
2854 1498 1301 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5954 7666 96
Size: 274 80 33 51 1443 3977 1712 138
Fragments arranged by size:
3977 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3968 4000 7668 7700 3968
Size: 32 3668 32 3976
Fragments arranged by size:
3976 3668 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7708
XbaI T'CTAG_A
Cuts at: 3080 5044 3080
Size: 1964 5744
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7708
XmaI C'CCGG_G
Cuts at: 1870 5033 1870
Size: 3163 4545
XmnI GAAnn'nnTTC
Cuts at: 2833 5837 2833
Size: 3004 4704
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5516 276
Size: 53 83 186 936 3982 2468
Fragments arranged by size:
3982 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SacI SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI BaeI BfrBI BsiWI BstXI BstZ17I
DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PspXI PsrI PvuII RsrII SacII SanDI
SexAI SrfI XcmI