(Circular) (Six-base) MAPSORT of: pFN21AE5008.seq Check: 473 from: 1 to: 5710 pFN21AE5008 5710 bp With 182 enzymes: SgfI * December 8, 2014 13:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2752 2752 Size: 5710 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3520 278 Size: 53 83 186 936 1984 2468 Fragments arranged by size: 2468 1984 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3053 1937 Size: 1116 4594 Acc65I G'GTAC_C Cuts at: 3031 3031 Size: 5710 AclI AA'CG_TT Cuts at: 1563 3837 4210 1563 Size: 2274 373 3063 Fragments arranged by size: 3063 2274 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2286 2531 2683 3773 5157 5425 1632 Size: 654 245 152 1090 1384 268 1917 Fragments arranged by size: 1917 1384 1090 654 268 245 152 AfeI AGC'GCT Cuts at: 2426 2426 Size: 5710 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5513 AflIII A'CryG_T Cuts at: 1203 4609 5586 1203 Size: 3406 977 1327 Fragments arranged by size: 3406 1327 977 AgeI A'CCGG_T Cuts at: 4541 4541 Size: 5710 AhdI GACnn_n'nnGTC Cuts at: 2197 4439 2197 Size: 2242 3468 AleI CACnn'nnGTG Cuts at: 1166 2618 2930 1166 Size: 1452 312 3946 Fragments arranged by size: 3946 1452 312 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2948 1184 Size: 1764 3946 AlwNI CAG_nnn'CTG Cuts at: 2509 2542 2876 3006 3453 5025 2509 Size: 33 334 130 447 1572 3194 Fragments arranged by size: 3194 1572 447 334 130 33 ApaLI G'TGCA_C Cuts at: 3767 4923 3767 Size: 1156 4554 ApoI r'AATT_y Cuts at: 1493 2117 3019 3245 3397 5506 1493 Size: 624 902 226 152 2109 1697 Fragments arranged by size: 2109 1697 902 624 226 152 AseI AT'TA_AT Cuts at: 160 1032 4264 160 Size: 872 3232 1606 Fragments arranged by size: 3232 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 AvaI C'yCGr_G Cuts at: 1870 1943 3035 1870 Size: 73 1092 4545 Fragments arranged by size: 4545 1092 73 BamHI G'GATC_C Cuts at: 3040 3407 3040 Size: 367 5343 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3031 4486 618 Size: 325 217 627 1244 1455 1842 Fragments arranged by size: 1842 1455 1244 627 325 217 BanII G_rGCy'C Cuts at: 1391 2636 3029 1391 Size: 1245 393 4072 Fragments arranged by size: 4072 1245 393 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2418 928 Size: 971 519 4220 Fragments arranged by size: 4220 971 519 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2432 2466 3901 3935 1903 Size: 34 495 34 1435 34 3678 Fragments arranged by size: 3678 1435 495 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3602 4812 3602 Size: 1210 4500 BclI T'GATC_A Cuts at: 1548 1548 Size: 5710 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4321 136 Size: 107 122 71 631 605 2649 1525 Fragments arranged by size: 2649 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2062 2280 5705 2062 Size: 218 3425 2067 Fragments arranged by size: 3425 2067 218 BlpI GC'TnA_GC Cuts at: 3127 3127 Size: 5710 Bme1580I G_kGCm'C Cuts at: 1790 2803 3771 4927 1790 Size: 1013 968 1156 2573 Fragments arranged by size: 2573 1156 1013 968 BmgBI CAC'GTC Cuts at: 1318 2705 1318 Size: 1387 4323 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3441 4399 442 Size: 416 53 83 399 24 2024 958 1753 Fragments arranged by size: 2024 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5710 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2375 2825 4370 1239 Size: 1136 450 1545 2579 Fragments arranged by size: 2579 1545 1136 450 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2659 3835 4700 4998 2659 Size: 1176 865 298 3371 Fragments arranged by size: 3371 1176 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2184 2772 4373 882 Size: 617 685 588 1601 2219 Fragments arranged by size: 2219 1601 685 617 588 BsaAI yAC'GTr Cuts at: 493 1204 3501 493 Size: 711 2297 2702 Fragments arranged by size: 2702 2297 711 BsaBI GATnn'nnATC Cuts at: 5704 5704 Size: 5710 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3517 3899 4534 275 Size: 53 83 186 936 1984 382 635 1451 Fragments arranged by size: 1984 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2015 4143 4541 4815 4962 5373 2015 Size: 2128 398 274 147 411 2352 Fragments arranged by size: 2352 2128 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5485 Fragments arranged by size: 5485 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2051 1186 Size: 865 4845 BseYI C'CCAG_C Cuts at: 2585 2669 2869 4913 5409 2585 Size: 84 200 2044 496 2886 Fragments arranged by size: 2886 2044 496 200 84 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2276 2570 2642 1163 Size: 573 540 294 72 4231 Fragments arranged by size: 4231 573 540 294 72 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3160 3921 4949 1540 Size: 275 186 1159 761 1028 2301 Fragments arranged by size: 2301 1159 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3771 3856 4927 3771 Size: 85 1071 4554 Fragments arranged by size: 4554 1071 85 BsmI GAATG_Cn' Cuts at: 3221 3314 3221 Size: 93 5617 BsmBI CGTCTCn'nnnn_ Cuts at: 2150 2905 2150 Size: 755 4955 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2636 2803 3029 3771 3856 4927 1391 Size: 399 846 167 226 742 85 1071 2174 Fragments arranged by size: 2174 1071 846 742 399 226 167 85 BspEI T'CCGG_A Cuts at: 2015 5373 2015 Size: 3358 2352 BspHI T'CATG_A Cuts at: 3599 5329 3599 Size: 1730 3980 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2752 3065 844 Size: 1908 313 3489 Fragments arranged by size: 3489 1908 313 BsrBI CCG'CTC Cuts at: 1394 1823 3597 1394 Size: 429 1774 3507 Fragments arranged by size: 3507 1774 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2652 4205 4379 65 Size: 1183 1404 1553 174 1396 Fragments arranged by size: 1553 1404 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2274 4354 4541 1700 Size: 110 464 2080 187 2869 Fragments arranged by size: 2869 2080 464 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5710 BssHII G'CGCG_C Cuts at: 1925 2389 1925 Size: 464 5246 BssSI C'ACGA_G Cuts at: 2834 2959 3770 4782 2834 Size: 125 811 1012 3762 Fragments arranged by size: 3762 1012 811 125 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5710 BstBI TT'CG_AA Cuts at: 4514 4514 Size: 5710 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5710 BstXI CCAn_nnnn'nTGG Cuts at: 2593 2593 Size: 5710 BstYI r'GATC_y Cuts at: 1972 2062 2280 3040 3407 3792 3809 5261 5347 Size: 90 218 760 367 385 17 1452 86 Cuts at: 5347 5359 5705 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 760 385 367 346 218 90 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2628 513 Size: 552 939 624 3595 Fragments arranged by size: 3595 939 624 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2084 487 Size: 683 56 858 4113 Fragments arranged by size: 4113 858 683 56 BtsI GCAGTG_nn' Cuts at: 3226 4020 4040 3226 Size: 794 20 4896 Fragments arranged by size: 4896 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5710 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2635 2670 529 Size: 35 2071 35 3569 Fragments arranged by size: 3569 2071 35 35 DraI TTT'AAA Cuts at: 3014 3014 Size: 5710 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4717 809 Size: 507 3401 1802 Fragments arranged by size: 3401 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2544 2915 3157 Size: 54 1425 50 275 18 714 371 242 Cuts at: 3157 4046 5675 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 714 371 275 242 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3157 1537 Size: 275 1345 4090 Fragments arranged by size: 4090 1345 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3640 1353 Size: 2287 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2896 4297 4671 4817 387 Size: 1064 138 1307 1401 374 146 1280 Fragments arranged by size: 1401 1307 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2286 2375 2531 2683 2825 3773 4370 Size: 393 654 89 156 152 142 948 597 Cuts at: 4370 5157 5425 1239 Size: 787 268 1524 Fragments arranged by size: 1524 948 787 654 597 393 268 156 152 142 89 EcoO109I rG'GnC_Cy Cuts at: 2574 2666 2730 2574 Size: 92 64 5554 Fragments arranged by size: 5554 92 64 EcoRI G'AATT_C Cuts at: 2117 3019 3397 2117 Size: 902 378 4430 Fragments arranged by size: 4430 902 378 EcoRV GAT'ATC Cuts at: 2757 2757 Size: 5710 FspI TGC'GCA Cuts at: 4216 4216 Size: 5710 HaeII r_GCGC'y Cuts at: 1678 2428 4857 1678 Size: 750 2429 2531 Fragments arranged by size: 2531 2429 750 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2183 2215 2389 2421 4351 Size: 32 135 32 611 32 174 32 1930 Cuts at: 4351 4383 4425 4457 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1930 611 174 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3054 3300 669 Size: 988 281 1116 246 3079 Fragments arranged by size: 3079 1116 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5710 HpaI GTT'AAC Cuts at: 3300 3300 Size: 5710 KpnI G_GTAC'C Cuts at: 3035 3035 Size: 5710 MfeI C'AATT_G Cuts at: 2330 3309 2330 Size: 979 4731 MluI A'CGCG_T Cuts at: 5586 5586 Size: 5710 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2934 4824 5008 1305 Size: 1629 1890 184 2007 Fragments arranged by size: 2007 1890 1629 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3888 Fragments arranged by size: 3888 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2399 2618 2930 3668 4027 4186 518 Size: 648 1233 219 312 738 359 159 2042 Fragments arranged by size: 2042 1233 738 648 359 312 219 159 MspA1I CmG'CkG Cuts at: 1606 2164 2618 2970 3126 3803 4951 5196 1606 Size: 558 454 352 156 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 558 454 352 245 156 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4219 Fragments arranged by size: 4219 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5710 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5710 NotI GC'GGCC_GC Cuts at: 3157 3157 Size: 5710 NspI r_CATG'y Cuts at: 1132 1229 3068 4613 1132 Size: 97 1839 1545 2229 Fragments arranged by size: 2229 1839 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5710 PciI A'CATG_T Cuts at: 4609 4609 Size: 5710 PflMI CCAn_nnn'nTGG Cuts at: 1108 2876 1108 Size: 1768 3942 PfoI T'CCnGG_A Cuts at: 2121 2457 2490 2121 Size: 336 33 5341 Fragments arranged by size: 5341 336 33 PmeI GTTT'AAAC Cuts at: 3014 3014 Size: 5710 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3779 3811 5292 5324 3779 Size: 32 1481 32 4165 Fragments arranged by size: 4165 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2574 2666 2730 2574 Size: 92 64 5554 Fragments arranged by size: 5554 92 64 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5710 PsiI TTA'TAA Cuts at: 3280 3280 Size: 5710 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2822 2854 2822 Size: 32 5678 PstI C_TGCA'G Cuts at: 830 3062 830 Size: 2232 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5710 PvuII CAG'CTG Cuts at: 2164 2618 2164 Size: 454 5256 SalI G'TCGA_C Cuts at: 1936 3052 1936 Size: 1116 4594 SanDI GG'GwC_CC Cuts at: 2730 2730 Size: 5710 SbfI CC_TGCA'GG Cuts at: 3062 3062 Size: 5710 ScaI AGT'ACT Cuts at: 3958 5670 3958 Size: 1712 3998 SexAI A'CCwGG_T Cuts at: 2990 2990 Size: 5710 SfcI C'TryA_G Cuts at: 826 1045 3058 4193 4874 5065 826 Size: 219 2013 1135 681 191 1471 Fragments arranged by size: 2013 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5710 SmaI CCC'GGG Cuts at: 1872 3037 1872 Size: 1165 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2674 3814 4715 4977 820 Size: 197 926 731 1140 901 262 1553 Fragments arranged by size: 1553 1140 926 901 731 262 197 SnaBI TAC'GTA Cuts at: 493 3501 493 Size: 3008 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5710 SphI G_CATG'C Cuts at: 1132 3068 1132 Size: 1936 3774 SspI AAT'ATT Cuts at: 5 52 3634 5 Size: 47 3582 2081 Fragments arranged by size: 3582 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5710 StyI C'CwwG_G Cuts at: 513 1065 2004 2289 3149 513 Size: 552 939 285 860 3074 Fragments arranged by size: 3074 939 860 552 285 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2930 3737 3754 3907 4092 4537 325 Size: 1255 1350 807 17 153 185 445 1498 Fragments arranged by size: 1498 1350 1255 807 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3956 5668 96 Size: 274 80 33 51 1443 1979 1712 138 Fragments arranged by size: 1979 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5670 5702 5670 Size: 32 5678 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5710 XbaI T'CTAG_A Cuts at: 3046 3046 Size: 5710 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2935 2935 Size: 5710 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5710 XmaI C'CCGG_G Cuts at: 1870 3035 1870 Size: 1165 4545 XmnI GAAnn'nnTTC Cuts at: 2499 3839 2499 Size: 1340 4370 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3518 276 Size: 53 83 186 936 1984 2468 Fragments arranged by size: 2468 1984 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI