Restriction map for
pFN21AE5008
(Circular) (Six-base) MAPSORT of: pFN21AE5008.seq Check: 473 from: 1 to: 5710
pFN21AE5008 5710 bp
With 182 enzymes: SgfI *
December 8, 2014 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2752 2752
Size: 5710
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3520 278
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3053 1937
Size: 1116 4594
Acc65I G'GTAC_C
Cuts at: 3031 3031
Size: 5710
AclI AA'CG_TT
Cuts at: 1563 3837 4210 1563
Size: 2274 373 3063
Fragments arranged by size:
3063 2274 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2286 2531 2683 3773 5157 5425 1632
Size: 654 245 152 1090 1384 268 1917
Fragments arranged by size:
1917 1384 1090 654 268 245 152
AfeI AGC'GCT
Cuts at: 2426 2426
Size: 5710
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5513
AflIII A'CryG_T
Cuts at: 1203 4609 5586 1203
Size: 3406 977 1327
Fragments arranged by size:
3406 1327 977
AgeI A'CCGG_T
Cuts at: 4541 4541
Size: 5710
AhdI GACnn_n'nnGTC
Cuts at: 2197 4439 2197
Size: 2242 3468
AleI CACnn'nnGTG
Cuts at: 1166 2618 2930 1166
Size: 1452 312 3946
Fragments arranged by size:
3946 1452 312
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2948 1184
Size: 1764 3946
AlwNI CAG_nnn'CTG
Cuts at: 2509 2542 2876 3006 3453 5025 2509
Size: 33 334 130 447 1572 3194
Fragments arranged by size:
3194 1572 447 334 130 33
ApaLI G'TGCA_C
Cuts at: 3767 4923 3767
Size: 1156 4554
ApoI r'AATT_y
Cuts at: 1493 2117 3019 3245 3397 5506 1493
Size: 624 902 226 152 2109 1697
Fragments arranged by size:
2109 1697 902 624 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4264 160
Size: 872 3232 1606
Fragments arranged by size:
3232 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
AvaI C'yCGr_G
Cuts at: 1870 1943 3035 1870
Size: 73 1092 4545
Fragments arranged by size:
4545 1092 73
BamHI G'GATC_C
Cuts at: 3040 3407 3040
Size: 367 5343
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3031 4486 618
Size: 325 217 627 1244 1455 1842
Fragments arranged by size:
1842 1455 1244 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2636 3029 1391
Size: 1245 393 4072
Fragments arranged by size:
4072 1245 393
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2418 928
Size: 971 519 4220
Fragments arranged by size:
4220 971 519
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2432 2466 3901 3935 1903
Size: 34 495 34 1435 34 3678
Fragments arranged by size:
3678 1435 495 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3602 4812 3602
Size: 1210 4500
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5710
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4321 136
Size: 107 122 71 631 605 2649 1525
Fragments arranged by size:
2649 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2062 2280 5705 2062
Size: 218 3425 2067
Fragments arranged by size:
3425 2067 218
BlpI GC'TnA_GC
Cuts at: 3127 3127
Size: 5710
Bme1580I G_kGCm'C
Cuts at: 1790 2803 3771 4927 1790
Size: 1013 968 1156 2573
Fragments arranged by size:
2573 1156 1013 968
BmgBI CAC'GTC
Cuts at: 1318 2705 1318
Size: 1387 4323
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3441 4399 442
Size: 416 53 83 399 24 2024 958 1753
Fragments arranged by size:
2024 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5710
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2375 2825 4370 1239
Size: 1136 450 1545 2579
Fragments arranged by size:
2579 1545 1136 450
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2659 3835 4700 4998 2659
Size: 1176 865 298 3371
Fragments arranged by size:
3371 1176 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2184 2772 4373 882
Size: 617 685 588 1601 2219
Fragments arranged by size:
2219 1601 685 617 588
BsaAI yAC'GTr
Cuts at: 493 1204 3501 493
Size: 711 2297 2702
Fragments arranged by size:
2702 2297 711
BsaBI GATnn'nnATC
Cuts at: 5704 5704
Size: 5710
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3517 3899 4534 275
Size: 53 83 186 936 1984 382 635 1451
Fragments arranged by size:
1984 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2015 4143 4541 4815 4962 5373 2015
Size: 2128 398 274 147 411 2352
Fragments arranged by size:
2352 2128 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5485
Fragments arranged by size:
5485 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 1186
Size: 865 4845
BseYI C'CCAG_C
Cuts at: 2585 2669 2869 4913 5409 2585
Size: 84 200 2044 496 2886
Fragments arranged by size:
2886 2044 496 200 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2276 2570 2642 1163
Size: 573 540 294 72 4231
Fragments arranged by size:
4231 573 540 294 72
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3160 3921 4949 1540
Size: 275 186 1159 761 1028 2301
Fragments arranged by size:
2301 1159 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3771 3856 4927 3771
Size: 85 1071 4554
Fragments arranged by size:
4554 1071 85
BsmI GAATG_Cn'
Cuts at: 3221 3314 3221
Size: 93 5617
BsmBI CGTCTCn'nnnn_
Cuts at: 2150 2905 2150
Size: 755 4955
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2636 2803 3029 3771 3856 4927 1391
Size: 399 846 167 226 742 85 1071 2174
Fragments arranged by size:
2174 1071 846 742 399 226 167 85
BspEI T'CCGG_A
Cuts at: 2015 5373 2015
Size: 3358 2352
BspHI T'CATG_A
Cuts at: 3599 5329 3599
Size: 1730 3980
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2752 3065 844
Size: 1908 313 3489
Fragments arranged by size:
3489 1908 313
BsrBI CCG'CTC
Cuts at: 1394 1823 3597 1394
Size: 429 1774 3507
Fragments arranged by size:
3507 1774 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2652 4205 4379 65
Size: 1183 1404 1553 174 1396
Fragments arranged by size:
1553 1404 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2274 4354 4541 1700
Size: 110 464 2080 187 2869
Fragments arranged by size:
2869 2080 464 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5710
BssHII G'CGCG_C
Cuts at: 1925 2389 1925
Size: 464 5246
BssSI C'ACGA_G
Cuts at: 2834 2959 3770 4782 2834
Size: 125 811 1012 3762
Fragments arranged by size:
3762 1012 811 125
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5710
BstBI TT'CG_AA
Cuts at: 4514 4514
Size: 5710
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5710
BstXI CCAn_nnnn'nTGG
Cuts at: 2593 2593
Size: 5710
BstYI r'GATC_y
Cuts at: 1972 2062 2280 3040 3407 3792 3809 5261 5347
Size: 90 218 760 367 385 17 1452 86
Cuts at: 5347 5359 5705 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 760 385 367 346 218 90
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2628 513
Size: 552 939 624 3595
Fragments arranged by size:
3595 939 624 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2084 487
Size: 683 56 858 4113
Fragments arranged by size:
4113 858 683 56
BtsI GCAGTG_nn'
Cuts at: 3226 4020 4040 3226
Size: 794 20 4896
Fragments arranged by size:
4896 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5710
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2635 2670 529
Size: 35 2071 35 3569
Fragments arranged by size:
3569 2071 35 35
DraI TTT'AAA
Cuts at: 3014 3014
Size: 5710
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4717 809
Size: 507 3401 1802
Fragments arranged by size:
3401 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2544 2915 3157
Size: 54 1425 50 275 18 714 371 242
Cuts at: 3157 4046 5675 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 714 371 275 242 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3157 1537
Size: 275 1345 4090
Fragments arranged by size:
4090 1345 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3640 1353
Size: 2287 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2896 4297 4671 4817 387
Size: 1064 138 1307 1401 374 146 1280
Fragments arranged by size:
1401 1307 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2286 2375 2531 2683 2825 3773 4370
Size: 393 654 89 156 152 142 948 597
Cuts at: 4370 5157 5425 1239
Size: 787 268 1524
Fragments arranged by size:
1524 948 787 654 597 393 268 156
152 142 89
EcoO109I rG'GnC_Cy
Cuts at: 2574 2666 2730 2574
Size: 92 64 5554
Fragments arranged by size:
5554 92 64
EcoRI G'AATT_C
Cuts at: 2117 3019 3397 2117
Size: 902 378 4430
Fragments arranged by size:
4430 902 378
EcoRV GAT'ATC
Cuts at: 2757 2757
Size: 5710
FspI TGC'GCA
Cuts at: 4216 4216
Size: 5710
HaeII r_GCGC'y
Cuts at: 1678 2428 4857 1678
Size: 750 2429 2531
Fragments arranged by size:
2531 2429 750
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2183 2215 2389 2421 4351
Size: 32 135 32 611 32 174 32 1930
Cuts at: 4351 4383 4425 4457 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1930 611 174 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3054 3300 669
Size: 988 281 1116 246 3079
Fragments arranged by size:
3079 1116 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5710
HpaI GTT'AAC
Cuts at: 3300 3300
Size: 5710
KpnI G_GTAC'C
Cuts at: 3035 3035
Size: 5710
MfeI C'AATT_G
Cuts at: 2330 3309 2330
Size: 979 4731
MluI A'CGCG_T
Cuts at: 5586 5586
Size: 5710
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2934 4824 5008 1305
Size: 1629 1890 184 2007
Fragments arranged by size:
2007 1890 1629 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3888
Fragments arranged by size:
3888 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2399 2618 2930 3668 4027 4186 518
Size: 648 1233 219 312 738 359 159 2042
Fragments arranged by size:
2042 1233 738 648 359 312 219 159
MspA1I CmG'CkG
Cuts at: 1606 2164 2618 2970 3126 3803 4951 5196 1606
Size: 558 454 352 156 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 558 454 352 245 156
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4219
Fragments arranged by size:
4219 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5710
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5710
NotI GC'GGCC_GC
Cuts at: 3157 3157
Size: 5710
NspI r_CATG'y
Cuts at: 1132 1229 3068 4613 1132
Size: 97 1839 1545 2229
Fragments arranged by size:
2229 1839 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5710
PciI A'CATG_T
Cuts at: 4609 4609
Size: 5710
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2876 1108
Size: 1768 3942
PfoI T'CCnGG_A
Cuts at: 2121 2457 2490 2121
Size: 336 33 5341
Fragments arranged by size:
5341 336 33
PmeI GTTT'AAAC
Cuts at: 3014 3014
Size: 5710
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3779 3811 5292 5324 3779
Size: 32 1481 32 4165
Fragments arranged by size:
4165 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2574 2666 2730 2574
Size: 92 64 5554
Fragments arranged by size:
5554 92 64
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5710
PsiI TTA'TAA
Cuts at: 3280 3280
Size: 5710
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2822 2854 2822
Size: 32 5678
PstI C_TGCA'G
Cuts at: 830 3062 830
Size: 2232 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5710
PvuII CAG'CTG
Cuts at: 2164 2618 2164
Size: 454 5256
SalI G'TCGA_C
Cuts at: 1936 3052 1936
Size: 1116 4594
SanDI GG'GwC_CC
Cuts at: 2730 2730
Size: 5710
SbfI CC_TGCA'GG
Cuts at: 3062 3062
Size: 5710
ScaI AGT'ACT
Cuts at: 3958 5670 3958
Size: 1712 3998
SexAI A'CCwGG_T
Cuts at: 2990 2990
Size: 5710
SfcI C'TryA_G
Cuts at: 826 1045 3058 4193 4874 5065 826
Size: 219 2013 1135 681 191 1471
Fragments arranged by size:
2013 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5710
SmaI CCC'GGG
Cuts at: 1872 3037 1872
Size: 1165 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2674 3814 4715 4977 820
Size: 197 926 731 1140 901 262 1553
Fragments arranged by size:
1553 1140 926 901 731 262 197
SnaBI TAC'GTA
Cuts at: 493 3501 493
Size: 3008 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5710
SphI G_CATG'C
Cuts at: 1132 3068 1132
Size: 1936 3774
SspI AAT'ATT
Cuts at: 5 52 3634 5
Size: 47 3582 2081
Fragments arranged by size:
3582 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5710
StyI C'CwwG_G
Cuts at: 513 1065 2004 2289 3149 513
Size: 552 939 285 860 3074
Fragments arranged by size:
3074 939 860 552 285
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2930 3737 3754 3907 4092 4537 325
Size: 1255 1350 807 17 153 185 445 1498
Fragments arranged by size:
1498 1350 1255 807 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3956 5668 96
Size: 274 80 33 51 1443 1979 1712 138
Fragments arranged by size:
1979 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5670 5702 5670
Size: 32 5678
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5710
XbaI T'CTAG_A
Cuts at: 3046 3046
Size: 5710
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2935 2935
Size: 5710
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5710
XmaI C'CCGG_G
Cuts at: 1870 3035 1870
Size: 1165 4545
XmnI GAAnn'nnTTC
Cuts at: 2499 3839 2499
Size: 1340 4370
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3518 276
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SalI SanDI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI RsrII SacI SacII
SapI SfiI SfoI SrfI SwaI