Restriction map for
pFN21AE5006
(Circular) (Six-base) MAPSORT of: pFN21AE5006.seq Check: 8220 from: 1 to: 5668
pFN21AE5006 5668 bp
With 182 enzymes: SgfI *
December 8, 2014 13:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3478 278
Size: 53 83 186 936 1942 2468
Fragments arranged by size:
2468 1942 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3011 1937
Size: 1074 4594
Acc65I G'GTAC_C
Cuts at: 2453 2989 2453
Size: 536 5132
AclI AA'CG_TT
Cuts at: 1563 2958 3795 4168 1563
Size: 1395 837 373 3063
Fragments arranged by size:
3063 1395 837 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2642 2921 2927 3731 5115 5383 1632
Size: 1010 279 6 804 1384 268 1917
Fragments arranged by size:
1917 1384 1010 804 279 268 6
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5471
AflIII A'CryG_T
Cuts at: 1203 2367 4567 5544 1203
Size: 1164 2200 977 1327
Fragments arranged by size:
2200 1327 1164 977
AgeI A'CCGG_T
Cuts at: 4499 4499
Size: 5668
AhdI GACnn_n'nnGTC
Cuts at: 2694 4397 2694
Size: 1703 3965
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5668
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5668
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2645 2677 2645
Size: 32 5636
AlwNI CAG_nnn'CTG
Cuts at: 2015 3411 4983 2015
Size: 1396 1572 2700
Fragments arranged by size:
2700 1572 1396
ApaLI G'TGCA_C
Cuts at: 3725 4881 3725
Size: 1156 4512
ApoI r'AATT_y
Cuts at: 1493 2132 2202 2977 3203 3355 5464 1493
Size: 639 70 775 226 152 2109 1697
Fragments arranged by size:
2109 1697 775 639 226 152 70
AseI AT'TA_AT
Cuts at: 160 1032 2679 2903 4222 160
Size: 872 1647 224 1319 1606
Fragments arranged by size:
1647 1606 1319 872 224
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5668
AvaI C'yCGr_G
Cuts at: 1870 1943 2169 2993 1870
Size: 73 226 824 4545
Fragments arranged by size:
4545 824 226 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2437 2470 2437
Size: 33 5635
BamHI G'GATC_C
Cuts at: 2998 3365 2998
Size: 367 5301
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2453 2989 4444 618
Size: 325 217 627 666 536 1455 1842
Fragments arranged by size:
1842 1455 666 627 536 325 217
BanII G_rGCy'C
Cuts at: 1391 2170 2987 1391
Size: 779 817 4072
Fragments arranged by size:
4072 817 779
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2115 928
Size: 971 216 4481
Fragments arranged by size:
4481 971 216
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2675 2709 3859 3893 1903
Size: 34 738 34 1150 34 3678
Fragments arranged by size:
3678 1150 738 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3560 4770 3560
Size: 1210 4458
BclI T'GATC_A
Cuts at: 1548 2374 1548
Size: 826 4842
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4279 136
Size: 107 122 71 631 605 2607 1525
Fragments arranged by size:
2607 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2918 5663 2918
Size: 2745 2923
BlpI GC'TnA_GC
Cuts at: 3085 3085
Size: 5668
Bme1580I G_kGCm'C
Cuts at: 1790 3729 4885 1790
Size: 1939 1156 2573
Fragments arranged by size:
2573 1939 1156
BmgBI CAC'GTC
Cuts at: 1318 2368 1318
Size: 1050 4618
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3399 4357 442
Size: 416 53 83 399 24 1982 958 1753
Fragments arranged by size:
1982 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5668
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4328 1239
Size: 3089 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3793 4658 4956 3793
Size: 865 298 4505
Fragments arranged by size:
4505 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4331 882
Size: 617 2832 2219
Fragments arranged by size:
2832 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3459 493
Size: 711 2255 2702
Fragments arranged by size:
2702 2255 711
BsaBI GATnn'nnATC
Cuts at: 5662 5662
Size: 5668
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3475 3857 4492 275
Size: 53 83 186 936 1942 382 635 1451
Fragments arranged by size:
1942 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4101 4499 4773 4920 5331 4101
Size: 398 274 147 411 4438
Fragments arranged by size:
4438 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2405 2435 2645 2675 1178
Size: 30 165 30 1002 30 210 30 4171
Fragments arranged by size:
4171 1002 210 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2108 1186
Size: 922 4746
BseYI C'CCAG_C
Cuts at: 4871 5367 4871
Size: 496 5172
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5095
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3118 3879 4907 1540
Size: 275 186 1117 761 1028 2301
Fragments arranged by size:
2301 1117 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3729 3814 4885 3729
Size: 85 1071 4512
Fragments arranged by size:
4512 1071 85
BsmI GAATG_Cn'
Cuts at: 2294 3179 3272 2294
Size: 885 93 4690
Fragments arranged by size:
4690 885 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2170 2987 3729 3814 4885 1391
Size: 399 380 817 742 85 1071 2174
Fragments arranged by size:
2174 1071 817 742 399 380 85
BspEI T'CCGG_A
Cuts at: 5331 5331
Size: 5668
BspHI T'CATG_A
Cuts at: 2044 2142 3557 5287 2044
Size: 98 1415 1730 2425
Fragments arranged by size:
2425 1730 1415 98
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2938 3023 844
Size: 2094 85 3489
Fragments arranged by size:
3489 2094 85
BsrBI CCG'CTC
Cuts at: 1394 1823 3555 1394
Size: 429 1732 3507
Fragments arranged by size:
3507 1732 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2223 4163 4337 65
Size: 1183 975 1940 174 1396
Fragments arranged by size:
1940 1396 1183 975 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4312 4499 1700
Size: 110 2502 187 2869
Fragments arranged by size:
2869 2502 187 110
BsrGI T'GTAC_A
Cuts at: 96 2352 96
Size: 2256 3412
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5668
BssSI C'ACGA_G
Cuts at: 3728 4740 3728
Size: 1012 4656
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5668
BstBI TT'CG_AA
Cuts at: 4472 4472
Size: 5668
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5668
BstYI r'GATC_y
Cuts at: 1972 2011 2918 2998 3365 3750 3767 5219 5305
Size: 39 907 80 367 385 17 1452 86
Cuts at: 5305 5317 5663 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 907 385 367 346 86 80
39 17 12
Bsu36I CC'TnA_GG
Cuts at: 2016 2016
Size: 5668
BtgI C'CryG_G
Cuts at: 513 1065 2004 2183 513
Size: 552 939 179 3998
Fragments arranged by size:
3998 939 552 179
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4929
Fragments arranged by size:
4929 683 56
BtsI GCAGTG_nn'
Cuts at: 2355 3184 3978 3998 2355
Size: 829 794 20 4025
Fragments arranged by size:
4025 829 794 20
ClaI AT'CG_AT
Cuts at: 1554 2535 1554
Size: 981 4687
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5633
DraI TTT'AAA
Cuts at: 2972 2972
Size: 5668
DraIII CAC_nnn'GTG
Cuts at: 2363 2363
Size: 5668
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4675 809
Size: 507 3359 1802
Fragments arranged by size:
3359 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3115 4004 5633
Size: 54 1425 50 275 18 1285 889 1629
Cuts at: 5633 8
Size: 43
Fragments arranged by size:
1629 1425 1285 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3115 1537
Size: 275 1303 4090
Fragments arranged by size:
4090 1303 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2043 2274 3598 1353
Size: 690 231 1324 3423
Fragments arranged by size:
3423 1324 690 231
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4255 4629 4775 387
Size: 1064 138 2666 374 146 1280
Fragments arranged by size:
2666 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2642 2921 2927 3731 4328 5115 5383
Size: 393 1010 279 6 804 597 787 268
Cuts at: 5383 1239
Size: 1524
Fragments arranged by size:
1524 1010 804 787 597 393 279 268
6
EcoRI G'AATT_C
Cuts at: 2977 3355 2977
Size: 378 5290
FspI TGC'GCA
Cuts at: 4174 4174
Size: 5668
HaeII r_GCGC'y
Cuts at: 1678 4815 1678
Size: 3137 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2490 2522 2680 2712 4309
Size: 32 135 32 918 32 158 32 1597
Cuts at: 4309 4341 4383 4415 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1597 918 158 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3012 3258 669
Size: 988 281 1074 246 3079
Fragments arranged by size:
3079 1074 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5668
HpaI GTT'AAC
Cuts at: 3258 3258
Size: 5668
KpnI G_GTAC'C
Cuts at: 2457 2993 2457
Size: 536 5132
MfeI C'AATT_G
Cuts at: 3267 3267
Size: 5668
MluI A'CGCG_T
Cuts at: 5544 5544
Size: 5668
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4782 4966 1305
Size: 3477 184 2007
Fragments arranged by size:
3477 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3846
Fragments arranged by size:
3846 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2439 3626 3985 4144 518
Size: 648 1273 1187 359 159 2042
Fragments arranged by size:
2042 1273 1187 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2233 3084 3761 4909 5154 1606
Size: 627 851 677 1148 245 2120
Fragments arranged by size:
2120 1148 851 677 627 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2183 513
Size: 552 939 179 3998
Fragments arranged by size:
3998 939 552 179
NdeI CA'TA_TG
Cuts at: 387 2344 387
Size: 1957 3711
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5668
NotI GC'GGCC_GC
Cuts at: 3115 3115
Size: 5668
NspI r_CATG'y
Cuts at: 1132 1229 2825 3026 4571 1132
Size: 97 1596 201 1545 2229
Fragments arranged by size:
2229 1596 1545 201 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5668
PciI A'CATG_T
Cuts at: 4567 4567
Size: 5668
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2263 1108
Size: 1155 4513
PmeI GTTT'AAAC
Cuts at: 2972 2972
Size: 5668
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2645 2677 3737 3769 5250 5282 2645
Size: 32 1060 32 1481 32 3031
Fragments arranged by size:
3031 1481 1060 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5668
PsiI TTA'TAA
Cuts at: 3238 3238
Size: 5668
PspXI vC'TCGA_Gb
Cuts at: 2169 2169
Size: 5668
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2837 2869 2837
Size: 32 5636
PstI C_TGCA'G
Cuts at: 830 2039 2688 3020 830
Size: 1209 649 332 3478
Fragments arranged by size:
3478 1209 649 332
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5668
PvuII CAG'CTG
Cuts at: 2233 2233
Size: 5668
SalI G'TCGA_C
Cuts at: 1936 3010 1936
Size: 1074 4594
SbfI CC_TGCA'GG
Cuts at: 3020 3020
Size: 5668
ScaI AGT'ACT
Cuts at: 2402 3916 5628 2402
Size: 1514 1712 2442
Fragments arranged by size:
2442 1712 1514
SfcI C'TryA_G
Cuts at: 826 1045 2035 2289 2684 3016 4151 4832 5023
Size: 219 990 254 395 332 1135 681 191
Cuts at: 5023 826
Size: 1471
Fragments arranged by size:
1471 1135 990 681 395 332 254 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5668
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5668
SmaI CCC'GGG
Cuts at: 1872 2995 1872
Size: 1123 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2169 3772 4673 4935 820
Size: 197 926 226 1603 901 262 1553
Fragments arranged by size:
1603 1553 926 901 262 226 197
SnaBI TAC'GTA
Cuts at: 493 3459 493
Size: 2966 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5668
SphI G_CATG'C
Cuts at: 1132 3026 1132
Size: 1894 3774
SspI AAT'ATT
Cuts at: 5 52 3592 5
Size: 47 3540 2081
Fragments arranged by size:
3540 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5668
StyI C'CwwG_G
Cuts at: 513 1065 2004 2183 3107 513
Size: 552 939 179 924 3074
Fragments arranged by size:
3074 939 924 552 179
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2343 3695 3712 3865 4050 4495 325
Size: 1255 763 1352 17 153 185 445 1498
Fragments arranged by size:
1498 1352 1255 763 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2352 2400 3914
Size: 274 80 33 51 1443 375 48 1514
Cuts at: 3914 5626 96
Size: 1712 138
Fragments arranged by size:
1712 1514 1443 375 274 138 80 51
48 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2616 2648 5628 5660 2616
Size: 32 2980 32 2624
Fragments arranged by size:
2980 2624 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5668
XbaI T'CTAG_A
Cuts at: 3004 3004
Size: 5668
XhoI C'TCGA_G
Cuts at: 1943 2169 1943
Size: 226 5442
XmaI C'CCGG_G
Cuts at: 1870 2993 1870
Size: 1123 4545
XmnI GAAnn'nnTTC
Cuts at: 2583 3797 2583
Size: 1214 4454
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3476 276
Size: 53 83 186 936 1942 2468
Fragments arranged by size:
2468 1942 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspXI PsrI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI