Restriction map for
pFN21AE5005
(Circular) (Six-base) MAPSORT of: pFN21AE5005.seq Check: 5923 from: 1 to: 6106
pFN21AE5005 6106 bp
With 182 enzymes: SgfI *
December 8, 2014 13:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3006 3916 278
Size: 53 83 186 936 1470 910 2468
Fragments arranged by size:
2468 1470 936 910 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3449 1937
Size: 1512 4594
Acc65I G'GTAC_C
Cuts at: 2184 3427 2184
Size: 1243 4863
AclI AA'CG_TT
Cuts at: 1563 4233 4606 1563
Size: 2670 373 3063
Fragments arranged by size:
3063 2670 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2510 2696 3248 4169 5553 5821 1632
Size: 878 186 552 921 1384 268 1917
Fragments arranged by size:
1917 1384 921 878 552 268 186
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5909
AflIII A'CryG_T
Cuts at: 1203 2628 2710 5005 5982 1203
Size: 1425 82 2295 977 1327
Fragments arranged by size:
2295 1425 1327 977 82
AgeI A'CCGG_T
Cuts at: 2214 4937 2214
Size: 2723 3383
AhdI GACnn_n'nnGTC
Cuts at: 4835 4835
Size: 6106
AleI CACnn'nnGTG
Cuts at: 1166 2936 1166
Size: 1770 4336
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6106
AlwNI CAG_nnn'CTG
Cuts at: 3849 5421 3849
Size: 1572 4534
ApaI G_GGCC'C
Cuts at: 3111 3111
Size: 6106
ApaLI G'TGCA_C
Cuts at: 4163 5319 4163
Size: 1156 4950
ApoI r'AATT_y
Cuts at: 1493 2812 3296 3415 3641 3793 5902 1493
Size: 1319 484 119 226 152 2109 1697
Fragments arranged by size:
2109 1697 1319 484 226 152 119
AseI AT'TA_AT
Cuts at: 160 1032 4660 160
Size: 872 3628 1606
Fragments arranged by size:
3628 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6106
AvaI C'yCGr_G
Cuts at: 1870 1943 2371 3431 1870
Size: 73 428 1060 4545
Fragments arranged by size:
4545 1060 428 73
AvrII C'CTAG_G
Cuts at: 3103 3103
Size: 6106
BamHI G'GATC_C
Cuts at: 3436 3803 3436
Size: 367 5739
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2184 3427 4882 618
Size: 325 217 627 397 1243 1455 1842
Fragments arranged by size:
1842 1455 1243 627 397 325 217
BanII G_rGCy'C
Cuts at: 1391 2234 2649 3111 3425 1391
Size: 843 415 462 314 4072
Fragments arranged by size:
4072 843 462 415 314
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5135
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4297 4331 1903
Size: 34 50 34 2276 34 3678
Fragments arranged by size:
3678 2276 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3998 5208 3998
Size: 1210 4896
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6106
BfrBI ATG'CAT
Cuts at: 2008 2008
Size: 6106
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2448 4717 136
Size: 107 122 71 631 605 776 2269 1525
Fragments arranged by size:
2269 1525 776 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2141 6101 2141
Size: 3960 2146
BlpI GC'TnA_GC
Cuts at: 3523 3523
Size: 6106
Bme1580I G_kGCm'C
Cuts at: 1790 2992 3111 4167 5323 1790
Size: 1202 119 1056 1156 2573
Fragments arranged by size:
2573 1202 1156 1056 119
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6106
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3837 4795 442
Size: 416 53 83 399 24 2420 958 1753
Fragments arranged by size:
2420 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6106
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3205 3205
Size: 6106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3028 3275 4766 1239
Size: 1789 247 1491 2579
Fragments arranged by size:
2579 1789 1491 247
Bpu10I CC'TnA_GC
Cuts at: 2051 2051
Size: 6106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2996 4231 5096 5394 2996
Size: 1235 865 298 3708
Fragments arranged by size:
3708 1235 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4769 882
Size: 617 3270 2219
Fragments arranged by size:
3270 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3262 3897 493
Size: 711 2058 635 2702
Fragments arranged by size:
2702 2058 711 635
BsaBI GATnn'nnATC
Cuts at: 6100 6100
Size: 6106
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3003 3913 4295 4930
Size: 53 83 186 936 1470 910 382 635
Cuts at: 4930 275
Size: 1451
Fragments arranged by size:
1470 1451 936 910 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2214 2307 4539 4937 5211 5358 5769 2214
Size: 93 2232 398 274 147 411 2551
Fragments arranged by size:
2551 2232 411 398 274 147 93
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5881
Fragments arranged by size:
5881 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2083 2086 2194 2359 2870 3298 3383 1186
Size: 897 3 108 165 511 428 85 3909
Fragments arranged by size:
3909 897 511 428 165 108 85 3
BseYI C'CCAG_C
Cuts at: 2980 2985 3309 5309 5805 2980
Size: 5 324 2000 496 3281
Fragments arranged by size:
3281 2000 496 324 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3284 3323 1163
Size: 573 1548 39 3946
Fragments arranged by size:
3946 1548 573 39
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3199 3556 4317 5345 1540
Size: 275 186 1198 357 761 1028 2301
Fragments arranged by size:
2301 1198 1028 761 357 275 186
BsiHKAI G_wGCw'C
Cuts at: 2550 2649 2844 2925 3081 3261 4167 4252 5323
Size: 99 195 81 156 180 906 85 1071
Cuts at: 5323 2550
Size: 3333
Fragments arranged by size:
3333 1071 906 195 180 156 99 85
81
BsmI GAATG_Cn'
Cuts at: 3021 3617 3710 3021
Size: 596 93 5417
Fragments arranged by size:
5417 596 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2234 2550 2649 2844 2925 2992 3081
Size: 399 444 316 99 195 81 67 89
Cuts at: 3081 3111 3261 3425 4167 4252 5323 1391
Size: 30 150 164 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 444 399 316 195 164
150 99 89 85 81 67 30
BspEI T'CCGG_A
Cuts at: 5769 5769
Size: 6106
BspHI T'CATG_A
Cuts at: 3995 5725 3995
Size: 1730 4376
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3461 844
Size: 2617 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2028 2227 3373 3993 1394
Size: 429 205 199 1146 620 3507
Fragments arranged by size:
3507 1146 620 429 205 199
BsrDI GCAATG_nn'
Cuts at: 65 1248 4601 4775 65
Size: 1183 3353 174 1396
Fragments arranged by size:
3353 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2214 3198 4750 4937 1700
Size: 110 404 984 1552 187 2869
Fragments arranged by size:
2869 1552 984 404 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6106
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6106
BssSI C'ACGA_G
Cuts at: 4166 5178 4166
Size: 1012 5094
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3018 794
Size: 2224 3882
BstBI TT'CG_AA
Cuts at: 4910 4910
Size: 6106
BstEII G'GTnAC_C
Cuts at: 1184 2429 1184
Size: 1245 4861
BstXI CCAn_nnnn'nTGG
Cuts at: 2938 2938
Size: 6106
BstYI r'GATC_y
Cuts at: 1972 2141 3099 3436 3803 4188 4205 5657 5743
Size: 169 958 337 367 385 17 1452 86
Cuts at: 5743 5755 6101 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 958 385 367 346 337 169
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2964 3366 2964
Size: 402 5704
BtgI C'CryG_G
Cuts at: 513 1065 2620 513
Size: 552 1555 3999
Fragments arranged by size:
3999 1555 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2620 487
Size: 683 56 1394 3973
Fragments arranged by size:
3973 1394 683 56
BtsI GCAGTG_nn'
Cuts at: 2329 2908 3622 4416 4436 2329
Size: 579 714 794 20 3999
Fragments arranged by size:
3999 794 714 579 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6106
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6071
DraI TTT'AAA
Cuts at: 3410 3410
Size: 6106
DraIII CAC_nnn'GTG
Cuts at: 2437 3085 2437
Size: 648 5458
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5113 809
Size: 507 3797 1802
Fragments arranged by size:
3797 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2940 3196 3324
Size: 54 1425 50 275 18 1110 256 128
Cuts at: 3324 3553 4442 6071 8
Size: 229 889 1629 43
Fragments arranged by size:
1629 1425 1110 889 275 256 229 128
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3196 3553 1537
Size: 275 1384 357 4090
Fragments arranged by size:
4090 1384 357 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2060 2217 3059 3224 4036 1353
Size: 707 157 842 165 812 3423
Fragments arranged by size:
3423 842 812 707 165 157
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4693 5067 5213 387
Size: 1064 138 3104 374 146 1280
Fragments arranged by size:
3104 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2647 2647
Size: 6106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2510 2696 3028 3248 3275 4169 4766
Size: 393 878 186 332 220 27 894 597
Cuts at: 4766 5553 5821 1239
Size: 787 268 1524
Fragments arranged by size:
1524 894 878 787 597 393 332 268
220 186 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2176 2176
Size: 6106
EcoO109I rG'GnC_Cy
Cuts at: 2076 3107 3166 3331 2076
Size: 1031 59 165 4851
Fragments arranged by size:
4851 1031 165 59
EcoRI G'AATT_C
Cuts at: 2812 3415 3793 2812
Size: 603 378 5125
Fragments arranged by size:
5125 603 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2680 2680
Size: 6106
FspI TGC'GCA
Cuts at: 4612 4612
Size: 6106
HaeII r_GCGC'y
Cuts at: 1678 2197 2367 5253 1678
Size: 519 170 2886 2531
Fragments arranged by size:
2886 2531 519 170
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2846 2878 3359 3391 4747
Size: 32 135 32 1274 32 481 32 1356
Cuts at: 4747 4779 4821 4853 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1356 1274 481 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3450 3696 669
Size: 988 281 1512 246 3079
Fragments arranged by size:
3079 1512 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6106
HpaI GTT'AAC
Cuts at: 3696 3696
Size: 6106
KpnI G_GTAC'C
Cuts at: 2188 3431 2188
Size: 1243 4863
MfeI C'AATT_G
Cuts at: 3705 3705
Size: 6106
MluI A'CGCG_T
Cuts at: 5982 5982
Size: 6106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5220 5404 1305
Size: 3915 184 2007
Fragments arranged by size:
3915 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2942 10
Size: 54 1425 343 1110 3174
Fragments arranged by size:
3174 1425 1110 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2215 2885 2936 4064 4423 4582 518
Size: 648 1049 670 51 1128 359 159 2042
Fragments arranged by size:
2042 1128 1049 670 648 359 159 51
MspA1I CmG'CkG
Cuts at: 1606 2770 3268 3522 4199 5347 5592 1606
Size: 1164 498 254 677 1148 245 2120
Fragments arranged by size:
2120 1164 1148 677 498 254 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5554
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6106
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6106
NotI GC'GGCC_GC
Cuts at: 3553 3553
Size: 6106
NsiI A_TGCA'T
Cuts at: 2010 2010
Size: 6106
NspI r_CATG'y
Cuts at: 1132 1229 2324 2632 2714 3241 3464 5009 1132
Size: 97 1095 308 82 527 223 1545 2229
Fragments arranged by size:
2229 1545 1095 527 308 223 97 82
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6106
PciI A'CATG_T
Cuts at: 2628 2710 5005 2628
Size: 82 2295 3729
Fragments arranged by size:
3729 2295 82
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2240 1108
Size: 1132 4974
PmeI GTTT'AAAC
Cuts at: 3410 3410
Size: 6106
PmlI CAC'GTG
Cuts at: 3262 3262
Size: 6106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4175 4207 5688 5720 4175
Size: 32 1481 32 4561
Fragments arranged by size:
4561 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2076 2076
Size: 6106
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6106
PsiI TTA'TAA
Cuts at: 3676 3676
Size: 6106
PspOMI G'GGCC_C
Cuts at: 3107 3107
Size: 6106
PstI C_TGCA'G
Cuts at: 830 3458 830
Size: 2628 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6106
PvuII CAG'CTG
Cuts at: 2770 3268 2770
Size: 498 5608
SacI G_AGCT'C
Cuts at: 2649 2649
Size: 6106
SalI G'TCGA_C
Cuts at: 1936 3448 1936
Size: 1512 4594
SapI GCTCTTCn'nnn_
Cuts at: 2217 2217
Size: 6106
SbfI CC_TGCA'GG
Cuts at: 3458 3458
Size: 6106
ScaI AGT'ACT
Cuts at: 3035 4354 6066 3035
Size: 1319 1712 3075
Fragments arranged by size:
3075 1712 1319
SexAI A'CCwGG_T
Cuts at: 2348 2433 3081 2348
Size: 85 648 5373
Fragments arranged by size:
5373 648 85
SfcI C'TryA_G
Cuts at: 826 1045 2080 3454 4589 5270 5461 826
Size: 219 1035 1374 1135 681 191 1471
Fragments arranged by size:
1471 1374 1135 1035 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6106
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6106
SmaI CCC'GGG
Cuts at: 1872 3433 1872
Size: 1561 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2371 2975 4210 5111 5373 820
Size: 197 926 428 604 1235 901 262 1553
Fragments arranged by size:
1553 1235 926 901 604 428 262 197
SnaBI TAC'GTA
Cuts at: 493 3897 493
Size: 3404 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6106
SphI G_CATG'C
Cuts at: 1132 2324 3464 1132
Size: 1192 1140 3774
Fragments arranged by size:
3774 1192 1140
SspI AAT'ATT
Cuts at: 5 52 3152 4030 5
Size: 47 3100 878 2081
Fragments arranged by size:
3100 2081 878 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6106
StyI C'CwwG_G
Cuts at: 513 1065 3103 3545 513
Size: 552 2038 442 3074
Fragments arranged by size:
3074 2038 552 442
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2136 4133 4150 4303 4488 4933 325
Size: 1255 556 1997 17 153 185 445 1498
Fragments arranged by size:
1997 1498 1255 556 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3033 4352 6064
Size: 274 80 33 51 1443 1056 1319 1712
Cuts at: 6064 96
Size: 138
Fragments arranged by size:
1712 1443 1319 1056 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3111 3143 6066 6098 3111
Size: 32 2923 32 3119
Fragments arranged by size:
3119 2923 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6106
XbaI T'CTAG_A
Cuts at: 3442 3442
Size: 6106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2884 2884
Size: 6106
XhoI C'TCGA_G
Cuts at: 1943 2371 1943
Size: 428 5678
XmaI C'CCGG_G
Cuts at: 1870 3431 1870
Size: 1561 4545
XmnI GAAnn'nnTTC
Cuts at: 2687 3152 4235 2687
Size: 465 1083 4558
Fragments arranged by size:
4558 1083 465
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3004 3914 276
Size: 53 83 186 936 1470 910 2468
Fragments arranged by size:
2468 1470 936 910 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BbvCI BsiWI
BsmBI BstZ17I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PspXI PsrI RsrII SacII
SanDI SfiI SfoI SrfI SwaI