Restriction map for
pFN21AE5003
(Circular) (Six-base) MAPSORT of: pFN21AE5003.seq Check: 6039 from: 1 to: 6106
pFN21AE5003 6106 bp
With 182 enzymes: SgfI *
December 8, 2014 13:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3916 278
Size: 53 83 186 936 2380 2468
Fragments arranged by size:
2468 2380 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2046 3449 1937
Size: 109 1403 4594
Fragments arranged by size:
4594 1403 109
Acc65I G'GTAC_C
Cuts at: 3427 3427
Size: 6106
AclI AA'CG_TT
Cuts at: 1563 4233 4606 1563
Size: 2670 373 3063
Fragments arranged by size:
3063 2670 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2189 2364 4169 5553 5821 1632
Size: 557 175 1805 1384 268 1917
Fragments arranged by size:
1917 1805 1384 557 268 175
AfeI AGC'GCT
Cuts at: 2273 2273
Size: 6106
AflII C'TTAA_G
Cuts at: 820 1017 2771 820
Size: 197 1754 4155
Fragments arranged by size:
4155 1754 197
AflIII A'CryG_T
Cuts at: 1203 2942 5005 5982 1203
Size: 1739 2063 977 1327
Fragments arranged by size:
2063 1739 1327 977
AgeI A'CCGG_T
Cuts at: 4937 4937
Size: 6106
AhdI GACnn_n'nnGTC
Cuts at: 4835 4835
Size: 6106
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6106
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6106
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2371 2403 2371
Size: 32 6074
AlwNI CAG_nnn'CTG
Cuts at: 2319 2557 2794 2878 3849 5421 2319
Size: 238 237 84 971 1572 3004
Fragments arranged by size:
3004 1572 971 238 237 84
ApaLI G'TGCA_C
Cuts at: 4163 5319 4163
Size: 1156 4950
ApoI r'AATT_y
Cuts at: 1493 2405 2471 2501 2540 2619 2927 3415 3641
Size: 912 66 30 39 79 308 488 226
Cuts at: 3641 3793 5902 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 912 488 308 226 152 79
66 39 30
AseI AT'TA_AT
Cuts at: 160 1032 4660 160
Size: 872 3628 1606
Fragments arranged by size:
3628 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6106
AvaI C'yCGr_G
Cuts at: 1870 1943 2583 3431 1870
Size: 73 640 848 4545
Fragments arranged by size:
4545 848 640 73
BamHI G'GATC_C
Cuts at: 3436 3803 3436
Size: 367 5739
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3427 4882 618
Size: 325 217 627 1640 1455 1842
Fragments arranged by size:
1842 1640 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3425 1391
Size: 2034 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2583 928
Size: 971 684 4451
Fragments arranged by size:
4451 971 684
BbvCI CC'TCA_GC
Cuts at: 2011 2011
Size: 6106
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4297 4331 1903
Size: 34 2360 34 3678
Fragments arranged by size:
3678 2360 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3998 5208 3998
Size: 1210 4896
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6106
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4717 136
Size: 107 122 71 631 605 3045 1525
Fragments arranged by size:
3045 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6101 6101
Size: 6106
BlpI GC'TnA_GC
Cuts at: 3523 3523
Size: 6106
Bme1580I G_kGCm'C
Cuts at: 1790 4167 5323 1790
Size: 2377 1156 2573
Fragments arranged by size:
2573 2377 1156
BmgBI CAC'GTC
Cuts at: 1318 3386 1318
Size: 2068 4038
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3837 4795 442
Size: 416 53 83 399 24 2420 958 1753
Fragments arranged by size:
2420 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2579 2900 3383 4766 1239
Size: 1340 321 483 1383 2579
Fragments arranged by size:
2579 1383 1340 483 321
Bpu10I CC'TnA_GC
Cuts at: 2011 2011
Size: 6106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2103 2456 4231 5096 5394 2103
Size: 353 1775 865 298 2815
Fragments arranged by size:
2815 1775 865 353 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2121 4769 882
Size: 617 622 2648 2219
Fragments arranged by size:
2648 2219 622 617
BsaAI yAC'GTr
Cuts at: 493 1204 3897 493
Size: 711 2693 2702
Fragments arranged by size:
2702 2693 711
BsaBI GATnn'nnATC
Cuts at: 6100 6100
Size: 6106
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3913 4295 4930 275
Size: 53 83 186 936 2380 382 635 1451
Fragments arranged by size:
2380 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4539 4937 5211 5358 5769 4539
Size: 398 274 147 411 4876
Fragments arranged by size:
4876 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2011 2041 2258 2288 2371
Size: 30 165 30 608 30 217 30 83
Cuts at: 2371 2401 1178
Size: 30 4883
Fragments arranged by size:
4883 608 217 165 83 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2016 1186
Size: 830 5276
BseYI C'CCAG_C
Cuts at: 2510 5309 5805 2510
Size: 2799 496 2811
Fragments arranged by size:
2811 2799 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5533
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3556 4317 5345 1540
Size: 275 186 1555 761 1028 2301
Fragments arranged by size:
2301 1555 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3094 4167 4252 5323 3094
Size: 1073 85 1071 3877
Fragments arranged by size:
3877 1073 1071 85
BsmI GAATG_Cn'
Cuts at: 3617 3710 3617
Size: 93 6013
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3094 3425 4167 4252 5323 1391
Size: 399 1304 331 742 85 1071 2174
Fragments arranged by size:
2174 1304 1071 742 399 331 85
BspEI T'CCGG_A
Cuts at: 5769 5769
Size: 6106
BspHI T'CATG_A
Cuts at: 3120 3995 5725 3120
Size: 875 1730 3501
Fragments arranged by size:
3501 1730 875
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3461 844
Size: 2617 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3993 1394
Size: 429 2170 3507
Fragments arranged by size:
3507 2170 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2407 2514 4601 4775 65
Size: 1183 1159 107 2087 174 1396
Fragments arranged by size:
2087 1396 1183 1159 174 107
BsrFI r'CCGG_y
Cuts at: 1700 1810 4750 4937 1700
Size: 110 2940 187 2869
Fragments arranged by size:
2940 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3225 96
Size: 3129 2977
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6106
BssSI C'ACGA_G
Cuts at: 4166 5178 4166
Size: 1012 5094
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6106
BstBI TT'CG_AA
Cuts at: 4910 4910
Size: 6106
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6106
BstYI r'GATC_y
Cuts at: 1972 2887 3358 3436 3803 4188 4205 5657 5743
Size: 915 471 78 367 385 17 1452 86
Cuts at: 5743 5755 6101 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 915 471 385 367 346 86
78 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5554
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5367
Fragments arranged by size:
5367 683 56
BtsI GCAGTG_nn'
Cuts at: 2216 3622 4416 4436 2216
Size: 1406 794 20 3886
Fragments arranged by size:
3886 1406 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6106
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6071
DraI TTT'AAA
Cuts at: 3410 3410
Size: 6106
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5113 809
Size: 507 3797 1802
Fragments arranged by size:
3797 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2447 3553 4442
Size: 54 1425 50 275 18 617 1106 889
Cuts at: 4442 6071 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1106 889 617 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3553 1537
Size: 275 1741 4090
Fragments arranged by size:
4090 1741 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2081 2846 4036 1353
Size: 728 765 1190 3423
Fragments arranged by size:
3423 1190 765 728
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4693 5067 5213 387
Size: 1064 138 3104 374 146 1280
Fragments arranged by size:
3104 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2189 2364 2579 2900 3383 4169 4766
Size: 393 557 175 215 321 483 786 597
Cuts at: 4766 5553 5821 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 786 597 557 483 393 321
268 215 175
EcoRI G'AATT_C
Cuts at: 2501 3415 3793 2501
Size: 914 378 4814
Fragments arranged by size:
4814 914 378
FspI TGC'GCA
Cuts at: 4612 4612
Size: 6106
HaeII r_GCGC'y
Cuts at: 1678 2275 5253 1678
Size: 597 2978 2531
Fragments arranged by size:
2978 2531 597
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2153 2185 2228 2260 2637
Size: 32 135 32 581 32 43 32 377
Cuts at: 2637 2669 4747 4779 4821 4853 1373
Size: 32 2078 32 42 32 2626
Fragments arranged by size:
2626 2078 581 377 135 43 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2139 3450 3696 669
Size: 988 281 201 1311 246 3079
Fragments arranged by size:
3079 1311 988 281 246 201
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6106
HpaI GTT'AAC
Cuts at: 2139 3696 2139
Size: 1557 4549
KpnI G_GTAC'C
Cuts at: 3431 3431
Size: 6106
MfeI C'AATT_G
Cuts at: 3705 3705
Size: 6106
MluI A'CGCG_T
Cuts at: 5982 5982
Size: 6106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2530 3425 5220 5404 1305
Size: 1225 895 1795 184 2007
Fragments arranged by size:
2007 1795 1225 895 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2449 10
Size: 54 1425 343 617 3667
Fragments arranged by size:
3667 1425 617 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2709 4064 4423 4582 518
Size: 648 1543 1355 359 159 2042
Fragments arranged by size:
2042 1543 1355 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2152 2854 3522 4199 5347 5592 1606
Size: 546 702 668 677 1148 245 2120
Fragments arranged by size:
2120 1148 702 677 668 546 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5554
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6106
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6106
NotI GC'GGCC_GC
Cuts at: 3553 3553
Size: 6106
NspI r_CATG'y
Cuts at: 1132 1229 2946 3464 5009 1132
Size: 97 1717 518 1545 2229
Fragments arranged by size:
2229 1717 1545 518 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6106
PciI A'CATG_T
Cuts at: 2942 5005 2942
Size: 2063 4043
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6106
PmeI GTTT'AAAC
Cuts at: 3410 3410
Size: 6106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2371 2403 2948 2980 4175 4207 5688 5720 2371
Size: 32 545 32 1195 32 1481 32 2757
Fragments arranged by size:
2757 1481 1195 545 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6106
PsiI TTA'TAA
Cuts at: 3676 3676
Size: 6106
PstI C_TGCA'G
Cuts at: 830 3458 830
Size: 2628 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6106
PvuII CAG'CTG
Cuts at: 2854 2854
Size: 6106
SalI G'TCGA_C
Cuts at: 1936 3448 1936
Size: 1512 4594
SbfI CC_TGCA'GG
Cuts at: 3458 3458
Size: 6106
ScaI AGT'ACT
Cuts at: 4354 6066 4354
Size: 1712 4394
SfcI C'TryA_G
Cuts at: 826 1045 3454 4589 5270 5461 826
Size: 219 2409 1135 681 191 1471
Fragments arranged by size:
2409 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6106
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6106
SmaI CCC'GGG
Cuts at: 1872 3433 1872
Size: 1561 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2118 2435 2771 4210 5111 5373
Size: 197 926 175 317 336 1439 901 262
Cuts at: 5373 820
Size: 1553
Fragments arranged by size:
1553 1439 926 901 336 317 262 197
175
SnaBI TAC'GTA
Cuts at: 493 3897 493
Size: 3404 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6106
SphI G_CATG'C
Cuts at: 1132 3464 1132
Size: 2332 3774
SspI AAT'ATT
Cuts at: 5 52 2674 2736 2988 4030 5
Size: 47 2622 62 252 1042 2081
Fragments arranged by size:
2622 2081 1042 252 62 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6106
StyI C'CwwG_G
Cuts at: 513 1065 2968 3545 513
Size: 552 1903 577 3074
Fragments arranged by size:
3074 1903 577 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4133 4150 4303 4488 4933 325
Size: 1255 2553 17 153 185 445 1498
Fragments arranged by size:
2553 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2869 3225 4352
Size: 274 80 33 51 1443 892 356 1127
Cuts at: 4352 6064 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1127 892 356 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6066 6098 6066
Size: 32 6074
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6106
XbaI T'CTAG_A
Cuts at: 3442 3442
Size: 6106
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6106
XmaI C'CCGG_G
Cuts at: 1870 3431 1870
Size: 1561 4545
XmnI GAAnn'nnTTC
Cuts at: 4235 4235
Size: 6106
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3914 276
Size: 53 83 186 936 2380 2468
Fragments arranged by size:
2468 2380 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI