Restriction map for
pFN21AE4592
(Circular) (Six-base) MAPSORT of: pFN21AE4592.seq Check: 1577 from: 1 to: 8278
pFN21AE4592 8278 bp
With 182 enzymes: SgfI *
July 6, 2012 17:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2926 3660 4051 4111 4150 5196 5202 2926
Size: 734 391 60 39 1046 6 6002
Fragments arranged by size:
6002 1046 734 391 60 39 6
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6088 278
Size: 53 83 186 936 4552 2468
Fragments arranged by size:
4552 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3284 5621 1937
Size: 1347 2337 4594
Fragments arranged by size:
4594 2337 1347
Acc65I G'GTAC_C
Cuts at: 5599 5599
Size: 8278
AclI AA'CG_TT
Cuts at: 1563 6405 6778 1563
Size: 4842 373 3063
Fragments arranged by size:
4842 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5573 6341 7725 7993 1632
Size: 3941 768 1384 268 1917
Fragments arranged by size:
3941 1917 1384 768 268
AfeI AGC'GCT
Cuts at: 5019 5462 5019
Size: 443 7835
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8081
AflIII A'CryG_T
Cuts at: 1203 5049 7177 8154 1203
Size: 3846 2128 977 1327
Fragments arranged by size:
3846 2128 1327 977
AgeI A'CCGG_T
Cuts at: 3814 7109 3814
Size: 3295 4983
AhdI GACnn_n'nnGTC
Cuts at: 2032 7007 2032
Size: 4975 3303
AleI CACnn'nnGTG
Cuts at: 1166 2840 1166
Size: 1674 6604
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8278
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4815 4847 4815
Size: 32 8246
AlwNI CAG_nnn'CTG
Cuts at: 5225 5488 6021 7593 5225
Size: 263 533 1572 5910
Fragments arranged by size:
5910 1572 533 263
ApaI G_GGCC'C
Cuts at: 2073 2468 2641 2744 2971 3053 3409 3534 3788
Size: 395 173 103 227 82 356 125 254
Cuts at: 3788 3915 4095 4113 4574 4998 2073
Size: 127 180 18 461 424 5353
Fragments arranged by size:
5353 461 424 395 356 254 227 180
173 127 125 103 82 18
ApaLI G'TGCA_C
Cuts at: 5234 6335 7491 5234
Size: 1101 1156 6021
Fragments arranged by size:
6021 1156 1101
ApoI r'AATT_y
Cuts at: 1493 5587 5813 5965 8074 1493
Size: 4094 226 152 2109 1697
Fragments arranged by size:
4094 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6832 160
Size: 872 5800 1606
Fragments arranged by size:
5800 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8278
AvaI C'yCGr_G
Cuts at: 1870 1943 2073 2175 3943 3983 4217 4744 4797
Size: 73 130 102 1768 40 234 527 53
Cuts at: 4797 5603 1870
Size: 806 4545
Fragments arranged by size:
4545 1768 806 527 234 130 102 73
53 40
AvrII C'CTAG_G
Cuts at: 2366 2366
Size: 8278
BamHI G'GATC_C
Cuts at: 4912 5280 5608 5975 4912
Size: 368 328 367 7215
Fragments arranged by size:
7215 368 367 328
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3796 3817 4228 5599 7054
Size: 325 217 627 2009 21 411 1371 1455
Cuts at: 7054 618
Size: 1842
Fragments arranged by size:
2009 1842 1455 1371 627 411 325 217
21
BanII G_rGCy'C
Cuts at: 1391 2073 2468 2604 2641 2744 2971 3053 3409
Size: 682 395 136 37 103 227 82 356
Cuts at: 3409 3534 3788 3915 3937 4073 4095 4113 4226
Size: 125 254 127 22 136 22 18 113
Cuts at: 4226 4376 4574 4752 4998 5597 1391
Size: 150 198 178 246 599 4072
Fragments arranged by size:
4072 682 599 395 356 254 246 227
198 178 150 136 136 127 125 113
103 82 37 22 22 18
BbeI G_GCGC'C
Cuts at: 3800 3800
Size: 8278
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2015 928
Size: 971 116 7191
Fragments arranged by size:
7191 971 116
BbvCI CC'TCA_GC
Cuts at: 4200 4200
Size: 8278
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6469 6503 1903
Size: 34 4532 34 3678
Fragments arranged by size:
4532 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3968 6170 7380 3968
Size: 2202 1210 4866
Fragments arranged by size:
4866 2202 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8278
BfrBI ATG'CAT
Cuts at: 2942 2942
Size: 8278
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2171 3690 3699
Size: 107 122 71 631 605 499 1519 9
Cuts at: 3699 3869 4959 6889 136
Size: 170 1090 1930 1525
Fragments arranged by size:
1930 1525 1519 1090 631 605 499 170
122 107 71 9
BglII A'GATC_T
Cuts at: 2229 8273 2229
Size: 6044 2234
BlpI GC'TnA_GC
Cuts at: 5061 5451 5695 5061
Size: 390 244 7644
Fragments arranged by size:
7644 390 244
Bme1580I G_kGCm'C
Cuts at: 1790 2073 2468 2641 2675 2744 2971 3053 3409
Size: 283 395 173 34 69 227 82 356
Cuts at: 3409 3534 3788 3915 4095 4113 4233 4574 4581
Size: 125 254 127 180 18 120 341 7
Cuts at: 4581 4626 4728 4998 5238 6339 7495 1790
Size: 45 102 270 240 1101 1156 2573
Fragments arranged by size:
2573 1156 1101 395 356 341 283 270
254 240 227 180 173 127 125 120
102 82 69 45 34 18 7
BmgBI CAC'GTC
Cuts at: 1318 2124 2397 1318
Size: 806 273 7199
Fragments arranged by size:
7199 806 273
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2397 3403 3411
Size: 416 53 83 399 24 980 1006 8
Cuts at: 3411 4568 6009 6967 442
Size: 1157 1441 958 1753
Fragments arranged by size:
1753 1441 1157 1006 980 958 416 399
83 53 24 8
BmtI G_CTAG'C
Cuts at: 1056 2550 1056
Size: 1494 6784
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2206 2248 2452 2206
Size: 42 204 8032
Fragments arranged by size:
8032 204 42
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2130 2823 3087 3564 3584 3690 4388 4632
Size: 891 693 264 477 20 106 698 244
Cuts at: 4632 5021 6938 1239
Size: 389 1917 2579
Fragments arranged by size:
2579 1917 891 698 693 477 389 264
244 106 20
Bpu10I CC'TnA_GC
Cuts at: 4169 4200 4533 4169
Size: 31 333 7914
Fragments arranged by size:
7914 333 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3729 3774 4829 6403 7268 7566 3729
Size: 45 1055 1574 865 298 4441
Fragments arranged by size:
4441 1574 1055 865 298 45
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2246 2920 3624 4205 4821 5271 6941
Size: 617 747 674 704 581 616 450 1670
Cuts at: 6941 882
Size: 2219
Fragments arranged by size:
2219 1670 747 704 674 617 616 581
450
BsaAI yAC'GTr
Cuts at: 493 1204 4723 6069 493
Size: 711 3519 1346 2702
Fragments arranged by size:
3519 2702 1346 711
BsaBI GATnn'nnATC
Cuts at: 8272 8272
Size: 8278
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3797 6085 6467 7102
Size: 53 83 186 936 2264 2288 382 635
Cuts at: 7102 275
Size: 1451
Fragments arranged by size:
2288 2264 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3814 4549 6711 7109 7383 7530 7941 3814
Size: 735 2162 398 274 147 411 4151
Fragments arranged by size:
4151 2162 735 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3046 3076 3310 3340 4815
Size: 30 165 30 1643 30 234 30 1475
Cuts at: 4815 4845 5153 5183 1178
Size: 30 308 30 4273
Fragments arranged by size:
4273 1643 1475 308 234 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2056 2240 2264 2619 3151 3169 3330 3528
Size: 870 184 24 355 532 18 161 198
Cuts at: 3528 4217 1186
Size: 689 5247
Fragments arranged by size:
5247 870 689 532 355 198 184 161
24 18
BseYI C'CCAG_C
Cuts at: 2467 3202 3221 4234 4277 5027 5290 7481 7977
Size: 735 19 1013 43 750 263 2191 496
Cuts at: 7977 2467
Size: 2768
Fragments arranged by size:
2768 2191 1013 750 735 496 263 43
19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4949 5174 5180 5315 5492 1163
Size: 573 3213 225 6 135 177 3949
Fragments arranged by size:
3949 3213 573 225 177 135 6
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5728 6489 7517 1540
Size: 275 186 3727 761 1028 2301
Fragments arranged by size:
3727 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2815 4549 5238 6339 6424 7495 2815
Size: 1734 689 1101 85 1071 3598
Fragments arranged by size:
3598 1734 1101 1071 689 85
BsiWI C'GTAC_G
Cuts at: 3921 3921
Size: 8278
BsmI GAATG_Cn'
Cuts at: 4562 5789 5882 4562
Size: 1227 93 6958
Fragments arranged by size:
6958 1227 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2468 2604 2641 2675 2744 2815
Size: 399 283 395 136 37 34 69 71
Cuts at: 2815 2971 3053 3409 3534 3788 3915 3937 4073
Size: 156 82 356 125 254 127 22 136
Cuts at: 4073 4095 4113 4226 4233 4376 4549 4574 4581
Size: 22 18 113 7 143 173 25 7
Cuts at: 4581 4626 4728 4752 4998 5238 5597 6339 6424
Size: 45 102 24 246 240 359 742 85
Cuts at: 6424 7495 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 395 359 356 283
254 246 240 173 156 143 136 136
127 125 113 102 85 82 71 69
45 37 34 25 24 22 22 18
7 7
BspEI T'CCGG_A
Cuts at: 7941 7941
Size: 8278
BspHI T'CATG_A
Cuts at: 6167 7897 6167
Size: 1730 6548
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2913 2926 3660 4051 4111 4150 5196 5202
Size: 2069 13 734 391 60 39 1046 6
Cuts at: 5202 5391 5633 844
Size: 189 242 3489
Fragments arranged by size:
3489 2069 1046 734 391 242 189 60
39 13 6
BsrBI CCG'CTC
Cuts at: 1394 1823 4468 5065 6165 1394
Size: 429 2645 597 1100 3507
Fragments arranged by size:
3507 2645 1100 597 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3215 3376 3858 5119 6773 6947 65
Size: 1183 1967 161 482 1261 1654 174 1396
Fragments arranged by size:
1967 1654 1396 1261 1183 482 174 161
BsrFI r'CCGG_y
Cuts at: 1700 1810 3814 4195 5386 6922 7109 1700
Size: 110 2004 381 1191 1536 187 2869
Fragments arranged by size:
2869 2004 1536 1191 381 187 110
BsrGI T'GTAC_A
Cuts at: 96 4776 96
Size: 4680 3598
BssHII G'CGCG_C
Cuts at: 1925 4338 4340 4439 1925
Size: 2413 2 99 5764
Fragments arranged by size:
5764 2413 99 2
BssSI C'ACGA_G
Cuts at: 4333 5333 6338 7350 4333
Size: 1000 1005 1012 5261
Fragments arranged by size:
5261 1012 1005 1000
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5225 5434 5488 5515 794
Size: 4431 209 54 27 3557
Fragments arranged by size:
4431 3557 209 54 27
BstBI TT'CG_AA
Cuts at: 7082 7082
Size: 8278
BstEII G'GTnAC_C
Cuts at: 1184 3826 1184
Size: 2642 5636
BstXI CCAn_nnnn'nTGG
Cuts at: 2842 2842
Size: 8278
BstYI r'GATC_y
Cuts at: 1972 2229 4912 5280 5608 5975 6360 6377 7829
Size: 257 2683 368 328 367 385 17 1452
Cuts at: 7829 7915 7927 8273 1972
Size: 86 12 346 1977
Fragments arranged by size:
2683 1977 1452 385 368 367 346 328
257 86 17 12
BstZ17I GTA'TAC
Cuts at: 3285 3285
Size: 8278
Bsu36I CC'TnA_GG
Cuts at: 4114 4156 5260 4114
Size: 42 1104 7132
Fragments arranged by size:
7132 1104 42
BtgI C'CryG_G
Cuts at: 513 1065 2595 3006 3852 3867 4080 513
Size: 552 1530 411 846 15 213 4711
Fragments arranged by size:
4711 1530 846 552 411 213 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4110 4568 487
Size: 683 56 2884 458 4197
Fragments arranged by size:
4197 2884 683 458 56
BtsI GCAGTG_nn'
Cuts at: 2137 4612 4743 4789 5489 5794 6588 6608 2137
Size: 2475 131 46 700 305 794 20 3807
Fragments arranged by size:
3807 2475 794 700 305 131 46 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8278
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3841 3876 529
Size: 35 3277 35 4931
Fragments arranged by size:
4931 3277 35 35
DraI TTT'AAA
Cuts at: 5582 5582
Size: 8278
DraIII CAC_nnn'GTG
Cuts at: 5515 5515
Size: 8278
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7285 809
Size: 507 5969 1802
Fragments arranged by size:
5969 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2297 3349 4383
Size: 54 1425 50 275 18 467 1052 1034
Cuts at: 4383 5725 6614 8243 8
Size: 1342 889 1629 43
Fragments arranged by size:
1629 1425 1342 1052 1034 889 467 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5725 1537
Size: 275 3913 4090
Fragments arranged by size:
4090 3913 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3100 3169 3187 3718 3724 3733 3739 3841
Size: 1747 69 18 531 6 9 6 102
Cuts at: 3841 6208 1353
Size: 2367 3423
Fragments arranged by size:
3423 2367 1747 531 102 69 18 9
6 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2350 6865 7239 7385 387
Size: 1064 138 761 4515 374 146 1280
Fragments arranged by size:
4515 1280 1064 761 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2130 2823 3087 3564 3584 3690 4388
Size: 393 498 693 264 477 20 106 698
Cuts at: 4388 4632 5021 5573 6341 6938 7725 7993 1239
Size: 244 389 552 768 597 787 268 1524
Fragments arranged by size:
1524 787 768 698 693 597 552 498
477 393 389 268 264 244 106 20
EcoNI CCTnn'n_nnAGG
Cuts at: 2459 4155 5555 2459
Size: 1696 1400 5182
Fragments arranged by size:
5182 1696 1400
EcoO109I rG'GnC_Cy
Cuts at: 2062 2070 2464 2637 2638 2705 2740 2741 2967
Size: 8 394 173 1 67 35 1 226
Cuts at: 2967 3049 3326 3383 3405 3531 3627 3692 3750
Size: 82 277 57 22 126 96 65 58
Cuts at: 3750 3784 3785 3912 3948 4011 4056 4085 4092
Size: 34 1 127 36 63 45 29 7
Cuts at: 4092 4109 4110 4637 4894 4933 4994 4995 5380
Size: 17 1 527 257 39 61 1 385
Cuts at: 5380 2062
Size: 4960
Fragments arranged by size:
4960 527 394 385 277 257 226 173
127 126 96 82 67 65 63 61
58 57 45 39 36 35 34 29
22 17 8 7 1 1 1 1
1
EcoRI G'AATT_C
Cuts at: 5587 5965 5587
Size: 378 7900
EcoRV GAT'ATC
Cuts at: 2374 2585 2374
Size: 211 8067
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4870 4870
Size: 8278
FspI TGC'GCA
Cuts at: 4658 6784 4658
Size: 2126 6152
HaeII r_GCGC'y
Cuts at: 1678 3110 3800 4176 5021 5464 5471 7425 1678
Size: 1432 690 376 845 443 7 1954 2531
Fragments arranged by size:
2531 1954 1432 845 690 443 376 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2018 2050 2426 2458 3310
Size: 32 135 32 446 32 376 32 852
Cuts at: 3310 3342 5140 5172 6919 6951 6993 7025 1373
Size: 32 1798 32 1747 32 42 32 2626
Fragments arranged by size:
2626 1798 1747 852 446 376 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2206 5622 5868 669
Size: 988 281 268 3416 246 3079
Fragments arranged by size:
3416 3079 988 281 268 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8278
HpaI GTT'AAC
Cuts at: 5868 5868
Size: 8278
KasI G'GCGC_C
Cuts at: 3796 3796
Size: 8278
KpnI G_GTAC'C
Cuts at: 5603 5603
Size: 8278
MfeI C'AATT_G
Cuts at: 5877 5877
Size: 8278
MluI A'CGCG_T
Cuts at: 8154 8154
Size: 8278
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2198 3747 7392 7576 1305
Size: 893 1549 3645 184 2007
Fragments arranged by size:
3645 2007 1549 893 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4385 10
Size: 54 1425 343 2553 3903
Fragments arranged by size:
3903 2553 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2840 6236 6595 6754 518
Size: 648 1674 3396 359 159 2042
Fragments arranged by size:
3396 2042 1674 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4793 5027 5075 5294 5425 5450 5506 5521
Size: 3187 234 48 219 131 25 56 15
Cuts at: 5521 5694 6371 7519 7764 1606
Size: 173 677 1148 245 2120
Fragments arranged by size:
3187 2120 1148 677 245 234 219 173
131 56 48 25 15
NaeI GCC'GGC
Cuts at: 4197 5388 4197
Size: 1191 7087
NarI GG'CG_CC
Cuts at: 3797 3797
Size: 8278
NcoI C'CATG_G
Cuts at: 513 1065 2595 3006 513
Size: 552 1530 411 5785
Fragments arranged by size:
5785 1530 552 411
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8278
NgoMIV G'CCGG_C
Cuts at: 4195 5386 4195
Size: 1191 7087
NheI G'CTAG_C
Cuts at: 1052 2546 1052
Size: 1494 6784
NotI GC'GGCC_GC
Cuts at: 5725 5725
Size: 8278
NsiI A_TGCA'T
Cuts at: 2944 2944
Size: 8278
NspI r_CATG'y
Cuts at: 1132 1229 5053 5495 5636 7181 1132
Size: 97 3824 442 141 1545 2229
Fragments arranged by size:
3824 2229 1545 442 141 97
PasI CC'CwG_GG
Cuts at: 1348 2066 2461 3613 3781 4052 4729 4898 5107
Size: 718 395 1152 168 271 677 169 209
Cuts at: 5107 1348
Size: 4519
Fragments arranged by size:
4519 1152 718 677 395 271 209 169
168
PciI A'CATG_T
Cuts at: 5049 7177 5049
Size: 2128 6150
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2846 2981 3007 3562 1108
Size: 1738 135 26 555 5824
Fragments arranged by size:
5824 1738 555 135 26
PfoI T'CCnGG_A
Cuts at: 3541 3541
Size: 8278
PmeI GTTT'AAAC
Cuts at: 5582 5582
Size: 8278
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4815 4847 6347 6379 7860 7892 4815
Size: 32 1500 32 1481 32 5201
Fragments arranged by size:
5201 1500 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2705 3326 3750 4011 4056 4085 4637 4894 5380
Size: 621 424 261 45 29 552 257 486
Cuts at: 5380 2705
Size: 5603
Fragments arranged by size:
5603 621 552 486 424 261 257 45
29
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8278
PsiI TTA'TAA
Cuts at: 5848 5848
Size: 8278
PspOMI G'GGCC_C
Cuts at: 2069 2464 2637 2740 2967 3049 3405 3530 3784
Size: 395 173 103 227 82 356 125 254
Cuts at: 3784 3911 4091 4109 4570 4994 2069
Size: 127 180 18 461 424 5353
Fragments arranged by size:
5353 461 424 395 356 254 227 180
173 127 125 103 82 18
PstI C_TGCA'G
Cuts at: 830 3154 4048 4947 5136 5430 5460 5630 830
Size: 2324 894 899 189 294 30 170 3478
Fragments arranged by size:
3478 2324 899 894 294 189 170 30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8278
PvuII CAG'CTG
Cuts at: 4793 5027 5075 5294 5425 5450 4793
Size: 234 48 219 131 25 7621
Fragments arranged by size:
7621 234 219 131 48 25
SalI G'TCGA_C
Cuts at: 1936 5620 1936
Size: 3684 4594
SanDI GG'GwC_CC
Cuts at: 2705 3326 4011 4894 2705
Size: 621 685 883 6089
Fragments arranged by size:
6089 883 685 621
SbfI CC_TGCA'GG
Cuts at: 4048 5630 4048
Size: 1582 6696
ScaI AGT'ACT
Cuts at: 6526 8238 6526
Size: 1712 6566
SexAI A'CCwGG_T
Cuts at: 2980 4401 2980
Size: 1421 6857
SfcI C'TryA_G
Cuts at: 826 1045 2410 3150 3679 4044 4617 4943 5132
Size: 219 1365 740 529 365 573 326 189
Cuts at: 5132 5426 5456 5542 5626 6761 7442 7633 826
Size: 294 30 86 84 1135 681 191 1471
Fragments arranged by size:
1471 1365 1135 740 681 573 529 365
326 294 219 191 189 86 84 30
SfiI GGCCn_nnn'nGGCC
Cuts at: 3699 4959 3699
Size: 1260 7018
SfoI GGC'GCC
Cuts at: 3798 3798
Size: 8278
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8278
SgrAI Cr'CCGG_yG
Cuts at: 1700 3814 1700
Size: 2114 6164
SmaI CCC'GGG
Cuts at: 1872 2075 3945 3985 4746 4799 5605 1872
Size: 203 1870 40 761 53 806 4545
Fragments arranged by size:
4545 1870 806 761 203 53 40
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3744 3789 4844 6382 7283 7545
Size: 197 926 1801 45 1055 1538 901 262
Cuts at: 7545 820
Size: 1553
Fragments arranged by size:
1801 1553 1538 1055 926 901 262 197
45
SnaBI TAC'GTA
Cuts at: 493 6069 493
Size: 5576 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8278
SphI G_CATG'C
Cuts at: 1132 5495 5636 1132
Size: 4363 141 3774
Fragments arranged by size:
4363 3774 141
SspI AAT'ATT
Cuts at: 5 52 6202 5
Size: 47 6150 2081
Fragments arranged by size:
6150 2081 47
StuI AGG'CCT
Cuts at: 1517 4119 1517
Size: 2602 5676
StyI C'CwwG_G
Cuts at: 513 1065 2366 2595 2709 3006 4260 5717 513
Size: 552 1301 229 114 297 1254 1457 3074
Fragments arranged by size:
3074 1457 1301 1254 552 297 229 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2499 2590 2667 2785 2834 3011 3438
Size: 1255 919 91 77 118 49 177 427
Cuts at: 3438 3573 6305 6322 6475 6660 7105 325
Size: 135 2732 17 153 185 445 1498
Fragments arranged by size:
2732 1498 1255 919 445 427 185 177
153 135 118 91 77 49 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4353 4776 6524
Size: 274 80 33 51 1443 2376 423 1748
Cuts at: 6524 8236 96
Size: 1712 138
Fragments arranged by size:
2376 1748 1712 1443 423 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5153 5185 8238 8270 5153
Size: 32 3053 32 5161
Fragments arranged by size:
5161 3053 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8278
XbaI T'CTAG_A
Cuts at: 5614 5614
Size: 8278
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2630 2978 2630
Size: 348 7930
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8278
XmaI C'CCGG_G
Cuts at: 1870 2073 3943 3983 4744 4797 5603 1870
Size: 203 1870 40 761 53 806 4545
Fragments arranged by size:
4545 1870 806 761 203 53 40
XmnI GAAnn'nnTTC
Cuts at: 5346 6407 5346
Size: 1061 7217
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6086 276
Size: 53 83 186 936 4552 2468
Fragments arranged by size:
4552 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI BaeI BsmBI EcoICRI FseI FspAI NruI PacI
PmlI PspXI PsrI RsrII SacI SacII SapI SrfI
SwaI