Restriction map for
pFN21AE4591
(Circular) (Six-base) MAPSORT of: pFN21AE4591.seq Check: 313 from: 1 to: 8026
pFN21AE4591 8026 bp
With 182 enzymes: SgfI *
March 28, 2012 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3376 3376
Size: 8026
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3792 4180 4605 5211
Size: 53 83 186 936 2256 388 425 606
Cuts at: 5211 5836 278
Size: 625 2468
Fragments arranged by size:
2468 2256 936 625 606 425 388 186
83 53
AccI GT'mk_AC
Cuts at: 1937 2748 4104 4395 5369 1937
Size: 811 1356 291 974 4594
Fragments arranged by size:
4594 1356 974 811 291
Acc65I G'GTAC_C
Cuts at: 2604 5347 2604
Size: 2743 5283
AclI AA'CG_TT
Cuts at: 1563 6153 6526 1563
Size: 4590 373 3063
Fragments arranged by size:
4590 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2855 3563 4103 6089 7473 7741 1632
Size: 1223 708 540 1986 1384 268 1917
Fragments arranged by size:
1986 1917 1384 1223 708 540 268
AfeI AGC'GCT
Cuts at: 2262 4427 5153 5183 2262
Size: 2165 726 30 5105
Fragments arranged by size:
5105 2165 726 30
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7829
AflIII A'CryG_T
Cuts at: 1203 6925 7902 1203
Size: 5722 977 1327
Fragments arranged by size:
5722 1327 977
AgeI A'CCGG_T
Cuts at: 3185 6857 3185
Size: 3672 4354
AhdI GACnn_n'nnGTC
Cuts at: 4348 6755 4348
Size: 2407 5619
AleI CACnn'nnGTG
Cuts at: 1166 2487 3623 1166
Size: 1321 1136 5569
Fragments arranged by size:
5569 1321 1136
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2940 5000 5273 1184
Size: 1756 2060 273 3937
Fragments arranged by size:
3937 2060 1756 273
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2679 2711 4817 4849 2679
Size: 32 2106 32 5856
Fragments arranged by size:
5856 2106 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3391 3892 3982 4060 4888 5290 5769 7341 3391
Size: 501 90 78 828 402 479 1572 4076
Fragments arranged by size:
4076 1572 828 501 479 402 90 78
ApaI G_GGCC'C
Cuts at: 2037 2059 2324 2349 2478 3235 2037
Size: 22 265 25 129 757 6828
Fragments arranged by size:
6828 757 265 129 25 22
ApaLI G'TGCA_C
Cuts at: 6083 7239 6083
Size: 1156 6870
ApoI r'AATT_y
Cuts at: 1493 5335 5561 5713 7822 1493
Size: 3842 226 152 2109 1697
Fragments arranged by size:
3842 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6580 160
Size: 872 5548 1606
Fragments arranged by size:
5548 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8026
AvaI C'yCGr_G
Cuts at: 1870 1943 2036 2477 2586 3228 3875 4739 5097
Size: 73 93 441 109 642 647 864 358
Cuts at: 5097 5351 1870
Size: 254 4545
Fragments arranged by size:
4545 864 647 642 441 358 254 109
93 73
BamHI G'GATC_C
Cuts at: 5356 5723 5356
Size: 367 7659
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2041 2604 3098 4050 4118
Size: 325 217 627 254 563 494 952 68
Cuts at: 4118 5347 6802 618
Size: 1229 1455 1842
Fragments arranged by size:
1842 1455 1229 952 627 563 494 325
254 217 68
BanII G_rGCy'C
Cuts at: 1391 2037 2059 2283 2324 2349 2478 2808 3051
Size: 646 22 224 41 25 129 330 243
Cuts at: 3051 3235 3771 4920 5345 1391
Size: 184 536 1149 425 4072
Fragments arranged by size:
4072 1149 646 536 425 330 243 224
184 129 41 25 22
BbeI G_GCGC'C
Cuts at: 4054 4122 4054
Size: 68 7958
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3953 5280 928
Size: 971 2054 1327 3674
Fragments arranged by size:
3674 2054 1327 971
BbvCI CC'TCA_GC
Cuts at: 3632 4311 3632
Size: 679 7347
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4612 4646 4919 4953 6217 6251 1903
Size: 34 2675 34 273 34 1264 34 3678
Fragments arranged by size:
3678 2675 1264 273 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3682 5137 5918 7128 3682
Size: 1455 781 1210 4580
Fragments arranged by size:
4580 1455 1210 781
BclI T'GATC_A
Cuts at: 1548 3180 4779 1548
Size: 1632 1599 4795
Fragments arranged by size:
4795 1632 1599
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2344 2567 2973
Size: 107 122 71 631 605 672 223 406
Cuts at: 2973 3565 4025 4293 4375 6637 136
Size: 592 460 268 82 2262 1525
Fragments arranged by size:
2262 1525 672 631 605 592 460 406
268 223 122 107 82 71
BglII A'GATC_T
Cuts at: 3012 4149 8021 3012
Size: 1137 3872 3017
Fragments arranged by size:
3872 3017 1137
BlpI GC'TnA_GC
Cuts at: 2942 5060 5443 2942
Size: 2118 383 5525
Fragments arranged by size:
5525 2118 383
Bme1580I G_kGCm'C
Cuts at: 1790 2037 2046 2059 2324 2349 2478 2616 2717
Size: 247 9 13 265 25 129 138 101
Cuts at: 2717 3088 3235 3630 4003 6087 7243 1790
Size: 371 147 395 373 2084 1156 2573
Fragments arranged by size:
2573 2084 1156 395 373 371 265 247
147 138 129 101 25 13 9
BmgBI CAC'GTC
Cuts at: 1318 2872 2887 3178 3469 1318
Size: 1554 15 291 291 5875
Fragments arranged by size:
5875 1554 291 291 15
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2562 3902 4354
Size: 416 53 83 399 24 1145 1340 452
Cuts at: 4354 5757 6715 442
Size: 1403 958 1753
Fragments arranged by size:
1753 1403 1340 1145 958 452 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8026
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2425 5173 2425
Size: 2748 5278
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2210 2822 2882 3593 4004 4166 4400 4511
Size: 971 612 60 711 411 162 234 111
Cuts at: 4511 4862 4898 5165 6686 1239
Size: 351 36 267 1521 2579
Fragments arranged by size:
2579 1521 971 711 612 411 351 267
234 162 111 60 36
Bpu10I CC'TnA_GC
Cuts at: 2985 2991 3632 4311 2985
Size: 6 641 679 6700
Fragments arranged by size:
6700 679 641 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3227 6151 7016 7314 3227
Size: 2924 865 298 3939
Fragments arranged by size:
3939 2924 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4563 6689 882
Size: 617 3064 2126 2219
Fragments arranged by size:
3064 2219 2126 617
BsaAI yAC'GTr
Cuts at: 493 1204 4705 4777 5817 493
Size: 711 3501 72 1040 2702
Fragments arranged by size:
3501 2702 1040 711 72
BsaBI GATnn'nnATC
Cuts at: 8020 8020
Size: 8026
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3789 4051 4119 4177
Size: 53 83 186 936 2256 262 68 58
Cuts at: 4177 4461 4602 5208 5833 6215 6850 275
Size: 284 141 606 625 382 635 1451
Fragments arranged by size:
2256 1451 936 635 625 606 382 284
262 186 141 83 68 58 53
BsaWI w'CCGG_w
Cuts at: 2760 2902 3185 6459 6857 7131 7278 7689 2760
Size: 142 283 3274 398 274 147 411 3097
Fragments arranged by size:
3274 3097 411 398 283 274 147 142
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2165 2195 2606 2636 3744
Size: 30 165 30 762 30 411 30 1108
Cuts at: 3744 3774 4202 4232 4259 4289 1178
Size: 30 428 30 27 30 4915
Fragments arranged by size:
4915 1108 762 428 411 165 30 30
30 30 30 30 30 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2123 2144 2180 2183 2186 3140 3805 4438
Size: 937 21 36 3 3 954 665 633
Cuts at: 4438 5162 1186
Size: 724 4050
Fragments arranged by size:
4050 954 937 724 665 633 36 21
3 3
BseYI C'CCAG_C
Cuts at: 2323 2455 2720 2809 2813 3022 3033 3675 4641
Size: 132 265 89 4 209 11 642 966
Cuts at: 4641 4751 7229 7725 2323
Size: 110 2478 496 2624
Fragments arranged by size:
2624 2478 966 642 496 265 209 132
110 89 11 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3128 3389 3491 3867 5183 5200 1163
Size: 573 1392 261 102 376 1316 17 3989
Fragments arranged by size:
3989 1392 1316 573 376 261 102 17
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2562 2968 4471 4567 4765 5476
Size: 275 186 561 406 1503 96 198 711
Cuts at: 5476 6237 7265 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1503 1028 761 711 561 406 275
198 186 96
BsiHKAI G_wGCw'C
Cuts at: 2379 2484 2585 2808 2943 4848 4989 5061 6087
Size: 105 101 223 135 1905 141 72 1026
Cuts at: 6087 6172 7243 2379
Size: 85 1071 3162
Fragments arranged by size:
3162 1905 1071 1026 223 141 135 105
101 85 72
BsmI GAATG_Cn'
Cuts at: 5143 5537 5630 5143
Size: 394 93 7539
Fragments arranged by size:
7539 394 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2684 2684
Size: 8026
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2037 2046 2059 2283 2324 2349 2379
Size: 399 247 9 13 224 41 25 30
Cuts at: 2379 2478 2484 2585 2616 2717 2808 2943 3051
Size: 99 6 101 31 101 91 135 108
Cuts at: 3051 3088 3235 3630 3771 4003 4848 4920 4989
Size: 37 147 395 141 232 845 72 69
Cuts at: 4989 5061 5345 6087 6172 7243 1391
Size: 72 284 742 85 1071 2174
Fragments arranged by size:
2174 1071 845 742 399 395 284 247
232 224 147 141 135 108 101 101
99 91 85 72 72 69 41 37
31 30 25 13 9 6
BspEI T'CCGG_A
Cuts at: 7689 7689
Size: 8026
BspHI T'CATG_A
Cuts at: 3834 5915 7645 3834
Size: 2081 1730 4215
Fragments arranged by size:
4215 2081 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2200 2502 3141 3376 3501 3564 3648 3649
Size: 1356 302 639 235 125 63 84 1
Cuts at: 3649 3999 4416 4623 5381 844
Size: 350 417 207 758 3489
Fragments arranged by size:
3489 1356 758 639 417 350 302 235
207 125 84 63 1
BsrBI CCG'CTC
Cuts at: 1394 1823 2995 3414 3577 5913 1394
Size: 429 1172 419 163 2336 3507
Fragments arranged by size:
3507 2336 1172 429 419 163
BsrDI GCAATG_nn'
Cuts at: 65 1248 2922 6521 6695 65
Size: 1183 1674 3599 174 1396
Fragments arranged by size:
3599 1674 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2557 2963 3185 3278 3330 4688 6670
Size: 110 747 406 222 93 52 1358 1982
Cuts at: 6670 6857 1700
Size: 187 2869
Fragments arranged by size:
2869 1982 1358 747 406 222 187 110
93 52
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8026
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8026
BssSI C'ACGA_G
Cuts at: 2660 3698 4520 5201 6086 7098 2660
Size: 1038 822 681 885 1012 3588
Fragments arranged by size:
3588 1038 1012 885 822 681
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3391 3694 4479 4852 5037 5046 5290 794
Size: 2597 303 785 373 185 9 244 3530
Fragments arranged by size:
3530 2597 785 373 303 244 185 9
BstBI TT'CG_AA
Cuts at: 6830 6830
Size: 8026
BstEII G'GTnAC_C
Cuts at: 1184 2392 2489 1184
Size: 1208 97 6721
Fragments arranged by size:
6721 1208 97
BstXI CCAn_nnnn'nTGG
Cuts at: 3838 4204 4378 4801 3838
Size: 366 174 423 7063
Fragments arranged by size:
7063 423 366 174
BstYI r'GATC_y
Cuts at: 1972 2230 2757 3012 3978 4149 5356 5723 6108
Size: 258 527 255 966 171 1207 367 385
Cuts at: 6108 6125 7577 7663 7675 8021 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1207 966 527 385 367 346
258 255 171 86 17 12
BstZ17I GTA'TAC
Cuts at: 4105 4105
Size: 8026
Bsu36I CC'TnA_GG
Cuts at: 2828 4575 5316 2828
Size: 1747 741 5538
Fragments arranged by size:
5538 1747 741
BtgI C'CryG_G
Cuts at: 513 1065 2004 2059 2359 2428 2608 2643 2912
Size: 552 939 55 300 69 180 35 269
Cuts at: 2912 3600 3717 4338 513
Size: 688 117 621 4201
Fragments arranged by size:
4201 939 688 621 552 300 269 180
117 69 55 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3781 4331 487
Size: 683 56 2555 550 4182
Fragments arranged by size:
4182 2555 683 550 56
BtsI GCAGTG_nn'
Cuts at: 2858 3560 4536 4544 5180 5542 6336 6356 2858
Size: 702 976 8 636 362 794 20 4528
Fragments arranged by size:
4528 976 794 702 636 362 20 8
ClaI AT'CG_AT
Cuts at: 1554 4632 1554
Size: 3078 4948
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7991
DraI TTT'AAA
Cuts at: 5330 5330
Size: 8026
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2210 7033 809
Size: 507 894 4823 1802
Fragments arranged by size:
4823 1802 894 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2021 2093 2514
Size: 54 1425 50 275 18 191 72 421
Cuts at: 2514 2559 2965 3276 3652 4026 4524 4713 4803
Size: 45 406 311 376 374 498 189 90
Cuts at: 4803 4890 5473 6362 7991 8
Size: 87 583 889 1629 43
Fragments arranged by size:
1629 1425 889 583 498 421 406 376
374 311 275 191 189 90 87 72
54 50 45 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2559 2965 5473 1537
Size: 275 747 406 2508 4090
Fragments arranged by size:
4090 2508 747 406 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3310 4001 4448 5956 1353
Size: 1957 691 447 1508 3423
Fragments arranged by size:
3423 1957 1508 691 447
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2882 6613 6987 7133 387
Size: 1064 138 1293 3731 374 146 1280
Fragments arranged by size:
3731 1293 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2806 2806
Size: 8026
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2210 2822 2855 2882 3563 3593 4004
Size: 393 578 612 33 27 681 30 411
Cuts at: 4004 4103 4166 4400 4511 4862 4898 5165 6089
Size: 99 63 234 111 351 36 267 924
Cuts at: 6089 6686 7473 7741 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 924 787 681 612 597 578 411
393 351 268 267 234 111 99 63
36 33 30 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2687 3137 3902 4928 5201 2687
Size: 450 765 1026 273 5512
Fragments arranged by size:
5512 1026 765 450 273
EcoO109I rG'GnC_Cy
Cuts at: 2033 2065 2113 2212 2345 2346 2998 3130 3921
Size: 32 48 99 133 1 652 132 791
Cuts at: 3921 4057 4486 4735 4825 5266 2033
Size: 136 429 249 90 441 4793
Fragments arranged by size:
4793 791 652 441 429 249 136 133
132 99 90 48 32 1
EcoRI G'AATT_C
Cuts at: 5335 5713 5335
Size: 378 7648
EcoRV GAT'ATC
Cuts at: 2953 4636 2953
Size: 1683 6343
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4414 4414
Size: 8026
FseI GG_CCGG'CC
Cuts at: 2561 3282 2561
Size: 721 7305
FspI TGC'GCA
Cuts at: 2636 4502 4907 5249 6532 2636
Size: 1866 405 342 1283 4130
Fragments arranged by size:
4130 1866 1283 405 342
HaeII r_GCGC'y
Cuts at: 1678 2264 2462 2651 3068 4054 4082 4095 4122
Size: 586 198 189 417 986 28 13 27
Cuts at: 4122 4429 5155 5185 5299 7173 1678
Size: 307 726 30 114 1874 2531
Fragments arranged by size:
2531 1874 986 726 586 417 307 198
189 114 30 28 27 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2165 2197 3744 3776 3800
Size: 32 135 32 593 32 1547 32 24
Cuts at: 3800 3832 3892 3924 4259 4291 4334 4366 4592
Size: 32 60 32 335 32 43 32 226
Cuts at: 4592 4624 6667 6699 6741 6773 1373
Size: 32 2043 32 42 32 2626
Fragments arranged by size:
2626 2043 1547 593 335 226 135 60
43 42 32 32 32 32 32 32
32 32 32 32 32 24
HincII GTy'rAC
Cuts at: 669 1657 1938 2749 5370 5616 669
Size: 988 281 811 2621 246 3079
Fragments arranged by size:
3079 2621 988 811 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8026
HpaI GTT'AAC
Cuts at: 5616 5616
Size: 8026
KasI G'GCGC_C
Cuts at: 4050 4118 4050
Size: 68 7958
KpnI G_GTAC'C
Cuts at: 2608 5351 2608
Size: 2743 5283
MfeI C'AATT_G
Cuts at: 5625 5625
Size: 8026
MluI A'CGCG_T
Cuts at: 7902 7902
Size: 8026
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2280 2317 3315 4704 4800 7140 7324 1305
Size: 975 37 998 1389 96 2340 184 2007
Fragments arranged by size:
2340 2007 1389 998 975 184 96 37
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2516 3654 4028 4526 4892
Size: 54 1425 343 684 1138 374 498 366
Cuts at: 4892 10
Size: 3144
Fragments arranged by size:
3144 1425 1138 684 498 374 366 343
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2487 2642 2699 3623 3836 5136 5984
Size: 648 1321 155 57 924 213 1300 848
Cuts at: 5984 6343 6502 518
Size: 359 159 2042
Fragments arranged by size:
2042 1321 1300 924 848 648 359 213
159 155 57
MspA1I CmG'CkG
Cuts at: 1606 2813 2860 3679 4075 4204 4340 4645 4831
Size: 1207 47 819 396 129 136 305 186
Cuts at: 4831 4888 5442 6119 7267 7512 1606
Size: 57 554 677 1148 245 2120
Fragments arranged by size:
2120 1207 1148 819 677 554 396 305
245 186 136 129 57 47
NaeI GCC'GGC
Cuts at: 2559 2965 3280 3332 4690 2559
Size: 406 315 52 1358 5895
Fragments arranged by size:
5895 1358 406 315 52
NarI GG'CG_CC
Cuts at: 4051 4119 4051
Size: 68 7958
NcoI C'CATG_G
Cuts at: 513 1065 2004 2912 513
Size: 552 939 908 5627
Fragments arranged by size:
5627 939 908 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8026
NgoMIV G'CCGG_C
Cuts at: 2557 2963 3278 3330 4688 2557
Size: 406 315 52 1358 5895
Fragments arranged by size:
5895 1358 406 315 52
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8026
NotI GC'GGCC_GC
Cuts at: 5473 5473
Size: 8026
NspI r_CATG'y
Cuts at: 1132 1229 2599 3382 5248 5384 6929 1132
Size: 97 1370 783 1866 136 1545 2229
Fragments arranged by size:
2229 1866 1545 1370 783 136 97
PasI CC'CwG_GG
Cuts at: 1348 2030 4324 1348
Size: 682 2294 5050
Fragments arranged by size:
5050 2294 682
PciI A'CATG_T
Cuts at: 6925 6925
Size: 8026
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2299 2317 3448 3982 4060 1108
Size: 1191 18 1131 534 78 5074
Fragments arranged by size:
5074 1191 1131 534 78 18
PfoI T'CCnGG_A
Cuts at: 2187 2682 3297 3981 4059 2187
Size: 495 615 684 78 6154
Fragments arranged by size:
6154 684 615 495 78
PmeI GTTT'AAAC
Cuts at: 5330 5330
Size: 8026
PmlI CAC'GTG
Cuts at: 4705 4705
Size: 8026
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2747 2779 3896 3928 6095 6127 7608 7640 2747
Size: 32 1117 32 2167 32 1481 32 3133
Fragments arranged by size:
3133 2167 1481 1117 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2113 2212 2998 3130 3921 4057 4735 4825 5266
Size: 99 786 132 791 136 678 90 441
Cuts at: 5266 2113
Size: 4873
Fragments arranged by size:
4873 791 786 678 441 136 132 99
90
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8026
PsiI TTA'TAA
Cuts at: 5596 5596
Size: 8026
PspOMI G'GGCC_C
Cuts at: 2033 2055 2320 2345 2474 3231 2033
Size: 22 265 25 129 757 6828
Fragments arranged by size:
6828 757 265 129 25 22
PstI C_TGCA'G
Cuts at: 830 2157 2181 3141 3387 3498 5378 830
Size: 1327 24 960 246 111 1880 3478
Fragments arranged by size:
3478 1880 1327 960 246 111 24
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8026
PvuII CAG'CTG
Cuts at: 2813 2860 3679 4075 4204 4645 4888 2813
Size: 47 819 396 129 441 243 5951
Fragments arranged by size:
5951 819 441 396 243 129 47
SacI G_AGCT'C
Cuts at: 2808 2808
Size: 8026
SacII CC_GC'GG
Cuts at: 4341 4341
Size: 8026
SalI G'TCGA_C
Cuts at: 1936 2747 5368 1936
Size: 811 2621 4594
Fragments arranged by size:
4594 2621 811
SanDI GG'GwC_CC
Cuts at: 2998 4735 2998
Size: 1737 6289
SapI GCTCTTCn'nnn_
Cuts at: 4448 4448
Size: 8026
SbfI CC_TGCA'GG
Cuts at: 5378 5378
Size: 8026
ScaI AGT'ACT
Cuts at: 4871 6274 7986 4871
Size: 1403 1712 4911
Fragments arranged by size:
4911 1712 1403
SexAI A'CCwGG_T
Cuts at: 4530 4530
Size: 8026
SfcI C'TryA_G
Cuts at: 826 1045 2153 2177 3137 3383 3494 4237 5374
Size: 219 1108 24 960 246 111 743 1137
Cuts at: 5374 6509 7190 7381 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1137 1135 1108 960 743 681 246
219 191 111 24
SfiI GGCCn_nnn'nGGCC
Cuts at: 4025 4025
Size: 8026
SfoI GGC'GCC
Cuts at: 4052 4120 4052
Size: 68 7958
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8026
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8026
SmaI CCC'GGG
Cuts at: 1872 2038 3877 4741 5099 5353 1872
Size: 166 1839 864 358 254 4545
Fragments arranged by size:
4545 1839 864 358 254 166
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3242 6130 7031 7293 820
Size: 197 926 1299 2888 901 262 1553
Fragments arranged by size:
2888 1553 1299 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5817 493
Size: 5324 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8026
SphI G_CATG'C
Cuts at: 1132 5384 1132
Size: 4252 3774
SspI AAT'ATT
Cuts at: 5 52 5950 5
Size: 47 5898 2081
Fragments arranged by size:
5898 2081 47
StuI AGG'CCT
Cuts at: 1517 2709 3143 5321 1517
Size: 1192 434 2178 4222
Fragments arranged by size:
4222 2178 1192 434
StyI C'CwwG_G
Cuts at: 513 1065 2004 2912 3102 3342 4014 4029 4251
Size: 552 939 908 190 240 672 15 222
Cuts at: 4251 4656 4794 5465 513
Size: 405 138 671 3074
Fragments arranged by size:
3074 939 908 672 671 552 405 240
222 190 138 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2477 2913 3086 4336 4581 6053 6070
Size: 1255 897 436 173 1250 245 1472 17
Cuts at: 6070 6223 6408 6853 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1472 1255 1250 897 445 436 245
185 173 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3402 3534 4218
Size: 274 80 33 51 1443 1425 132 684
Cuts at: 4218 4869 6272 7984 96
Size: 651 1403 1712 138
Fragments arranged by size:
1712 1443 1425 1403 684 651 274 138
132 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2606 2638 2861 2893 4604 4636 7986 8018 2606
Size: 32 223 32 1711 32 3350 32 2614
Fragments arranged by size:
3350 2614 1711 223 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2783 2888 3002 4277 4766 1316
Size: 1467 105 114 1275 489 4576
Fragments arranged by size:
4576 1467 1275 489 114 105
XbaI T'CTAG_A
Cuts at: 5362 5362
Size: 8026
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3802 4021 4061 4204 4252 3802
Size: 219 40 143 48 7576
Fragments arranged by size:
7576 219 143 48 40
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8026
XmaI C'CCGG_G
Cuts at: 1870 2036 3875 4739 5097 5351 1870
Size: 166 1839 864 358 254 4545
Fragments arranged by size:
4545 1839 864 358 254 166
XmnI GAAnn'nnTTC
Cuts at: 6155 6155
Size: 8026
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3790 4178 4603 5209
Size: 53 83 186 936 2256 388 425 606
Cuts at: 5209 5834 276
Size: 625 2468
Fragments arranged by size:
2468 2256 936 625 606 425 388 186
83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI DraIII FspAI NruI
NsiI PacI PspXI PsrI RsrII SrfI SwaI