Restriction map for
pFN21AE4582
(Circular) (Six-base) MAPSORT of: pFN21AE4582.seq Check: 9239 from: 1 to: 7156
pFN21AE4582 7156 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3361 3739 4027 3361
Size: 378 288 6490
Fragments arranged by size:
6490 378 288
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4966 278
Size: 53 83 186 936 3430 2468
Fragments arranged by size:
3430 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3459 4499 1937
Size: 1522 1040 4594
Fragments arranged by size:
4594 1522 1040
Acc65I G'GTAC_C
Cuts at: 3881 4477 3881
Size: 596 6560
AclI AA'CG_TT
Cuts at: 1563 4224 5283 5656 1563
Size: 2661 1059 373 3063
Fragments arranged by size:
3063 2661 1059 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5219 6603 6871 1632
Size: 3587 1384 268 1917
Fragments arranged by size:
3587 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6959
AflIII A'CryG_T
Cuts at: 1203 6055 7032 1203
Size: 4852 977 1327
Fragments arranged by size:
4852 1327 977
AgeI A'CCGG_T
Cuts at: 5987 5987
Size: 7156
AhdI GACnn_n'nnGTC
Cuts at: 5885 5885
Size: 7156
AleI CACnn'nnGTG
Cuts at: 1166 2543 3161 1166
Size: 1377 618 5161
Fragments arranged by size:
5161 1377 618
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2069 1184
Size: 885 6271
AlwNI CAG_nnn'CTG
Cuts at: 2617 2693 3784 4042 4373 4400 4899 6471 2617
Size: 76 1091 258 331 27 499 1572 3302
Fragments arranged by size:
3302 1572 1091 499 331 258 76 27
ApaLI G'TGCA_C
Cuts at: 4337 5213 6369 4337
Size: 876 1156 5124
Fragments arranged by size:
5124 1156 876
ApoI r'AATT_y
Cuts at: 1493 2826 4465 4691 4843 6952 1493
Size: 1333 1639 226 152 2109 1697
Fragments arranged by size:
2109 1697 1639 1333 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5710 160
Size: 872 4678 1606
Fragments arranged by size:
4678 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7156
AvaI C'yCGr_G
Cuts at: 1870 1943 2250 2280 2522 2970 3045 3352 4443
Size: 73 307 30 242 448 75 307 1091
Cuts at: 4443 4481 1870
Size: 38 4545
Fragments arranged by size:
4545 1091 448 307 307 242 75 73
38 30
BamHI G'GATC_C
Cuts at: 4486 4853 4486
Size: 367 6789
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2312 2754 3050 3881 3919
Size: 325 217 627 525 442 296 831 38
Cuts at: 3919 4477 5932 618
Size: 558 1455 1842
Fragments arranged by size:
1842 1455 831 627 558 525 442 325
296 217 38
BanII G_rGCy'C
Cuts at: 1391 2140 2258 2363 3728 4475 1391
Size: 749 118 105 1365 747 4072
Fragments arranged by size:
4072 1365 749 747 118 105
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2245 4132 4171 928
Size: 971 346 1887 39 3913
Fragments arranged by size:
3913 1887 971 346 39
BbvCI CC'TCA_GC
Cuts at: 3492 3492
Size: 7156
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5347 5381 1903
Size: 34 3410 34 3678
Fragments arranged by size:
3678 3410 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2287 5048 6258 2287
Size: 2761 1210 3185
Fragments arranged by size:
3185 2761 1210
BclI T'GATC_A
Cuts at: 1548 2547 3804 1548
Size: 999 1257 4900
Fragments arranged by size:
4900 1257 999
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5767 136
Size: 107 122 71 631 605 4095 1525
Fragments arranged by size:
4095 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2730 7151 2730
Size: 4421 2735
BlpI GC'TnA_GC
Cuts at: 2357 3092 3727 4573 2357
Size: 735 635 846 4940
Fragments arranged by size:
4940 846 735 635
Bme1580I G_kGCm'C
Cuts at: 1790 2540 2759 4341 5217 6373 1790
Size: 750 219 1582 876 1156 2573
Fragments arranged by size:
2573 1582 1156 876 750 219
BmgBI CAC'GTC
Cuts at: 1318 4021 1318
Size: 2703 4453
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2194 2549 2951
Size: 416 53 83 399 24 777 355 402
Cuts at: 2951 4106 4887 5845 442
Size: 1155 781 958 1753
Fragments arranged by size:
1753 1155 958 781 777 416 402 399
355 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7156
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2061 2191 2325 3296 4422 5816 1239
Size: 822 130 134 971 1126 1394 2579
Fragments arranged by size:
2579 1394 1126 971 822 134 130
Bpu10I CC'TnA_GC
Cuts at: 3492 3492
Size: 7156
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2519 5281 6146 6444 2519
Size: 2762 865 298 3231
Fragments arranged by size:
3231 2762 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2946 3796 3890 4418 5819 882
Size: 617 1447 850 94 528 1401 2219
Fragments arranged by size:
2219 1447 1401 850 617 528 94
BsaAI yAC'GTr
Cuts at: 493 1204 2545 2776 4947 493
Size: 711 1341 231 2171 2702
Fragments arranged by size:
2702 2171 1341 711 231
BsaBI GATnn'nnATC
Cuts at: 2735 3803 7150 2735
Size: 1068 3347 2741
Fragments arranged by size:
3347 2741 1068
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4963 5345 5980 275
Size: 53 83 186 936 3430 382 635 1451
Fragments arranged by size:
3430 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5589 5987 6261 6408 6819 5589
Size: 398 274 147 411 5926
Fragments arranged by size:
5926 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2059 2089 2690 2720 2943
Size: 30 165 30 656 30 601 30 223
Cuts at: 2943 2973 3194 3224 4092 4122 1178
Size: 30 221 30 868 30 4212
Fragments arranged by size:
4212 868 656 601 223 221 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2130 2133 2444 2447 3575 1186
Size: 944 3 311 3 1128 4767
Fragments arranged by size:
4767 1128 944 311 3 3
BseYI C'CCAG_C
Cuts at: 2693 3135 4366 6359 6855 2693
Size: 442 1231 1993 496 2994
Fragments arranged by size:
2994 1993 1231 496 442
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3824 1163
Size: 573 2088 4495
Fragments arranged by size:
4495 2088 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4606 5367 6395 1540
Size: 275 186 2605 761 1028 2301
Fragments arranged by size:
2605 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2363 2391 3282 4341 5217 5302 6373 2363
Size: 28 891 1059 876 85 1071 3146
Fragments arranged by size:
3146 1071 1059 891 876 85 28
BsmI GAATG_Cn'
Cuts at: 4667 4760 4667
Size: 93 7063
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2140 2258 2363 2391 2540 2759 3282
Size: 399 350 118 105 28 149 219 523
Cuts at: 3282 3728 4341 4475 5217 5302 6373 1391
Size: 446 613 134 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 613 523 446 399 350
219 149 134 118 105 85 28
BspEI T'CCGG_A
Cuts at: 6819 6819
Size: 7156
BspHI T'CATG_A
Cuts at: 5045 6775 5045
Size: 1730 5426
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2631 3361 3739 4027 4511 844
Size: 1787 730 378 288 484 3489
Fragments arranged by size:
3489 1787 730 484 378 288
BsrBI CCG'CTC
Cuts at: 1394 1823 2860 3664 4032 5043 1394
Size: 429 1037 804 368 1011 3507
Fragments arranged by size:
3507 1037 1011 804 429 368
BsrDI GCAATG_nn'
Cuts at: 65 1248 2731 4249 4302 5651 5825 65
Size: 1183 1483 1518 53 1349 174 1396
Fragments arranged by size:
1518 1483 1396 1349 1183 174 53
BsrFI r'CCGG_y
Cuts at: 1700 1810 2268 2922 3969 5800 5987 1700
Size: 110 458 654 1047 1831 187 2869
Fragments arranged by size:
2869 1831 1047 654 458 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7156
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7156
BssSI C'ACGA_G
Cuts at: 5216 6228 5216
Size: 1012 6144
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3414 794
Size: 2620 4536
BstBI TT'CG_AA
Cuts at: 5960 5960
Size: 7156
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7156
BstXI CCAn_nnnn'nTGG
Cuts at: 3066 3751 3952 4435 3066
Size: 685 201 483 5787
Fragments arranged by size:
5787 685 483 201
BstYI r'GATC_y
Cuts at: 1972 2730 3084 3343 3781 4215 4486 4853 5238
Size: 758 354 259 438 434 271 367 385
Cuts at: 5238 5255 6707 6793 6805 7151 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 758 438 434 385 367 354
346 271 259 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2431 2887 2947 2985 3240 3691 513
Size: 552 1366 456 60 38 255 451 3978
Fragments arranged by size:
3978 1366 552 456 451 255 60 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6417
Fragments arranged by size:
6417 683 56
BtsI GCAGTG_nn'
Cuts at: 2578 3409 4113 4256 4672 5466 5486 2578
Size: 831 704 143 416 794 20 4248
Fragments arranged by size:
4248 831 794 704 416 143 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7156
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2587 2622 529
Size: 35 2023 35 5063
Fragments arranged by size:
5063 2023 35 35
DraI TTT'AAA
Cuts at: 4460 4460
Size: 7156
DraIII CAC_nnn'GTG
Cuts at: 2893 2893
Size: 7156
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3011 6163 809
Size: 507 1695 3152 1802
Fragments arranged by size:
3152 1802 1695 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2875 3127 3183
Size: 54 1425 50 275 18 1045 252 56
Cuts at: 3183 3741 4044 4114 4603 5492 7121 8
Size: 558 303 70 489 889 1629 43
Fragments arranged by size:
1629 1425 1045 889 558 489 303 275
252 70 56 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4603 1537
Size: 275 2791 4090
Fragments arranged by size:
4090 2791 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2747 3329 5086 1353
Size: 1394 582 1757 3423
Fragments arranged by size:
3423 1757 1394 582
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3202 5743 6117 6263 387
Size: 1064 138 1613 2541 374 146 1280
Fragments arranged by size:
2541 1613 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2361 2361
Size: 7156
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2061 2191 2325 3296 4422 5219 5816
Size: 393 429 130 134 971 1126 797 597
Cuts at: 5816 6603 6871 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1126 971 797 787 597 429 393
268 134 130
EcoO109I rG'GnC_Cy
Cuts at: 2189 3927 4289 2189
Size: 1738 362 5056
Fragments arranged by size:
5056 1738 362
EcoRI G'AATT_C
Cuts at: 4465 4843 4465
Size: 378 6778
FspI TGC'GCA
Cuts at: 4070 5662 4070
Size: 1592 5564
HaeII r_GCGC'y
Cuts at: 1678 6303 1678
Size: 4625 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2090 2122 2943 2975 3102
Size: 32 135 32 518 32 821 32 127
Cuts at: 3102 3134 3328 3335 3360 3367 4413 4445 5797
Size: 32 194 7 25 7 1046 32 1352
Cuts at: 5797 5829 5871 5903 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1352 1046 821 518 194 135 127
42 32 32 32 32 32 32 32
32 25 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 4500 4746 669
Size: 988 281 2562 246 3079
Fragments arranged by size:
3079 2562 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7156
HpaI GTT'AAC
Cuts at: 4746 4746
Size: 7156
KpnI G_GTAC'C
Cuts at: 3885 4481 3885
Size: 596 6560
MfeI C'AATT_G
Cuts at: 4755 4755
Size: 7156
MluI A'CGCG_T
Cuts at: 7032 7032
Size: 7156
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3630 6270 6454 1305
Size: 2325 2640 184 2007
Fragments arranged by size:
2640 2325 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3129 3185 3743 4046 4116
Size: 54 1425 343 1297 56 558 303 70
Cuts at: 4116 10
Size: 3050
Fragments arranged by size:
3050 1425 1297 558 343 303 70 56
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2543 2555 3064 3161 3749 3950 4352
Size: 648 1377 12 509 97 588 201 402
Cuts at: 4352 4433 5114 5473 5632 518
Size: 81 681 359 159 2042
Fragments arranged by size:
2042 1377 681 648 588 509 402 359
201 159 97 81 12
MspA1I CmG'CkG
Cuts at: 1606 2289 2303 2433 3523 3731 4572 5249 6397
Size: 683 14 130 1090 208 841 677 1148
Cuts at: 6397 6642 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1090 841 683 677 245 208
130 14
NaeI GCC'GGC
Cuts at: 2270 2270
Size: 7156
NcoI C'CATG_G
Cuts at: 513 1065 2985 3240 513
Size: 552 1920 255 4429
Fragments arranged by size:
4429 1920 552 255
NdeI CA'TA_TG
Cuts at: 387 2448 2938 387
Size: 2061 490 4605
Fragments arranged by size:
4605 2061 490
NgoMIV G'CCGG_C
Cuts at: 2268 2268
Size: 7156
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7156
NotI GC'GGCC_GC
Cuts at: 4603 4603
Size: 7156
NspI r_CATG'y
Cuts at: 1132 1229 2332 3613 4514 6059 1132
Size: 97 1103 1281 901 1545 2229
Fragments arranged by size:
2229 1545 1281 1103 901 97
PasI CC'CwG_GG
Cuts at: 1348 4373 1348
Size: 3025 4131
PciI A'CATG_T
Cuts at: 6055 6055
Size: 7156
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3982 4373 1108
Size: 2874 391 3891
Fragments arranged by size:
3891 2874 391
PfoI T'CCnGG_A
Cuts at: 2465 2916 2465
Size: 451 6705
PmeI GTTT'AAAC
Cuts at: 4460 4460
Size: 7156
PmlI CAC'GTG
Cuts at: 2545 2545
Size: 7156
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5225 5257 6738 6770 5225
Size: 32 1481 32 5611
Fragments arranged by size:
5611 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2189 2189
Size: 7156
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7156
PsiI TTA'TAA
Cuts at: 4726 4726
Size: 7156
PstI C_TGCA'G
Cuts at: 830 4508 830
Size: 3678 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7156
PvuII CAG'CTG
Cuts at: 2303 2303
Size: 7156
RsrII CG'GwC_CG
Cuts at: 3934 3934
Size: 7156
SacI G_AGCT'C
Cuts at: 2363 2363
Size: 7156
SacII CC_GC'GG
Cuts at: 2434 2434
Size: 7156
SalI G'TCGA_C
Cuts at: 1936 4498 1936
Size: 2562 4594
SbfI CC_TGCA'GG
Cuts at: 4508 4508
Size: 7156
ScaI AGT'ACT
Cuts at: 5404 7116 5404
Size: 1712 5444
SfcI C'TryA_G
Cuts at: 826 1045 4504 5639 6320 6511 826
Size: 219 3459 1135 681 191 1471
Fragments arranged by size:
3459 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7156
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7156
SmaI CCC'GGG
Cuts at: 1872 2252 2282 2972 4483 1872
Size: 380 30 690 1511 4545
Fragments arranged by size:
4545 1511 690 380 30
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2498 4443 5260 6161 6423 820
Size: 197 926 555 1945 817 901 262 1553
Fragments arranged by size:
1945 1553 926 901 817 555 262 197
SnaBI TAC'GTA
Cuts at: 493 4947 493
Size: 4454 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7156
SphI G_CATG'C
Cuts at: 1132 2332 4514 1132
Size: 1200 2182 3774
Fragments arranged by size:
3774 2182 1200
SspI AAT'ATT
Cuts at: 5 52 5080 5
Size: 47 5028 2081
Fragments arranged by size:
5028 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7156
StyI C'CwwG_G
Cuts at: 513 1065 2985 3240 4595 513
Size: 552 1920 255 1355 3074
Fragments arranged by size:
3074 1920 1355 552 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2855 3058 3354 3657 5183 5200 5353
Size: 1255 1275 203 296 303 1526 17 153
Cuts at: 5353 5538 5983 325
Size: 185 445 1498
Fragments arranged by size:
1526 1498 1275 1255 445 303 296 203
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3513 4129 5402
Size: 274 80 33 51 1443 1536 616 1273
Cuts at: 5402 7114 96
Size: 1712 138
Fragments arranged by size:
1712 1536 1443 1273 616 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2059 2091 4092 4124 7116 7148 2059
Size: 32 2001 32 2992 32 2067
Fragments arranged by size:
2992 2067 2001 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2957 1316
Size: 1641 5515
XbaI T'CTAG_A
Cuts at: 4492 4492
Size: 7156
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3907 3907
Size: 7156
XhoI C'TCGA_G
Cuts at: 1943 4443 1943
Size: 2500 4656
XmaI C'CCGG_G
Cuts at: 1870 2250 2280 2970 4481 1870
Size: 380 30 690 1511 4545
Fragments arranged by size:
4545 1511 690 380 30
XmnI GAAnn'nnTTC
Cuts at: 5285 5285
Size: 7156
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4964 276
Size: 53 83 186 936 3430 2468
Fragments arranged by size:
3430 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
RsrII SacI SacII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PspOMI
PspXI PsrI SanDI SapI SexAI SfiI SfoI SrfI
SwaI