Restriction map for
pFN21AE4581
(Circular) (Six-base) MAPSORT of: pFN21AE4581.seq Check: 2074 from: 1 to: 7120
pFN21AE4581 7120 bp
With 182 enzymes: SgfI *
July 6, 2012 17:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3232 3232
Size: 7120
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3792 4930 278
Size: 53 83 186 936 2256 1138 2468
Fragments arranged by size:
2468 2256 1138 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3492 4463 1937
Size: 1555 971 4594
Fragments arranged by size:
4594 1555 971
Acc65I G'GTAC_C
Cuts at: 4371 4441 4371
Size: 70 7050
AclI AA'CG_TT
Cuts at: 1563 5247 5620 1563
Size: 3684 373 3063
Fragments arranged by size:
3684 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4148 5183 6567 6835 1632
Size: 2516 1035 1384 268 1917
Fragments arranged by size:
2516 1917 1384 1035 268
AfeI AGC'GCT
Cuts at: 2381 2381
Size: 7120
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6923
AflIII A'CryG_T
Cuts at: 1203 3048 4179 6019 6996 1203
Size: 1845 1131 1840 977 1327
Fragments arranged by size:
1845 1840 1327 1131 977
AgeI A'CCGG_T
Cuts at: 5951 5951
Size: 7120
AhdI GACnn_n'nnGTC
Cuts at: 3967 5849 3967
Size: 1882 5238
AleI CACnn'nnGTG
Cuts at: 1166 3905 1166
Size: 2739 4381
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7120
AlwNI CAG_nnn'CTG
Cuts at: 2882 2971 3325 4036 4863 6435 2882
Size: 89 354 711 827 1572 3567
Fragments arranged by size:
3567 1572 827 711 354 89
ApaI G_GGCC'C
Cuts at: 4294 4294
Size: 7120
ApaLI G'TGCA_C
Cuts at: 4201 5177 6333 4201
Size: 976 1156 4988
Fragments arranged by size:
4988 1156 976
ApoI r'AATT_y
Cuts at: 1493 3019 4429 4655 4807 6916 1493
Size: 1526 1410 226 152 2109 1697
Fragments arranged by size:
2109 1697 1526 1410 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5674 160
Size: 872 4642 1606
Fragments arranged by size:
4642 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7120
AvaI C'yCGr_G
Cuts at: 1870 1943 4445 1870
Size: 73 2502 4545
Fragments arranged by size:
4545 2502 73
BamHI G'GATC_C
Cuts at: 2406 4450 4817 2406
Size: 2044 367 4709
Fragments arranged by size:
4709 2044 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2065 4116 4246 4305 4371
Size: 325 217 627 278 2051 130 59 66
Cuts at: 4371 4441 5896 618
Size: 70 1455 1842
Fragments arranged by size:
2051 1842 1455 627 325 278 217 130
70 66 59
BanII G_rGCy'C
Cuts at: 1391 2576 2914 3338 3525 4294 4404 4439 1391
Size: 1185 338 424 187 769 110 35 4072
Fragments arranged by size:
4072 1185 769 424 338 187 110 35
BbeI G_GCGC'C
Cuts at: 2069 4120 2069
Size: 2051 5069
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3127 3535 3784 928
Size: 971 1228 408 249 4264
Fragments arranged by size:
4264 1228 971 408 249
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5311 5345 1903
Size: 34 3374 34 3678
Fragments arranged by size:
3678 3374 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5012 6222 5012
Size: 1210 5910
BclI T'GATC_A
Cuts at: 1548 2688 1548
Size: 1140 5980
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3275 4024 5731
Size: 107 122 71 631 605 1603 749 1707
Cuts at: 5731 136
Size: 1525
Fragments arranged by size:
1707 1603 1525 749 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2826 7115 2826
Size: 4289 2831
BlpI GC'TnA_GC
Cuts at: 3956 4537 3956
Size: 581 6539
Bme1580I G_kGCm'C
Cuts at: 1790 3840 4205 4251 4294 5181 6337 1790
Size: 2050 365 46 43 887 1156 2573
Fragments arranged by size:
2573 2050 1156 887 365 46 43
BmgBI CAC'GTC
Cuts at: 1318 3952 1318
Size: 2634 4486
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3859 4851 5809
Size: 416 53 83 399 24 2442 992 958
Cuts at: 5809 442
Size: 1753
Fragments arranged by size:
2442 1753 992 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7120
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3400 4420 3400
Size: 1020 6100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2348 2998 4013 5780 1239
Size: 1109 650 1015 1767 2579
Fragments arranged by size:
2579 1767 1109 1015 650
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2264 2450 2660 5245 6110 6408 2264
Size: 186 210 2585 865 298 2976
Fragments arranged by size:
2976 2585 865 298 210 186
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5783 882
Size: 617 4284 2219
Fragments arranged by size:
4284 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2665 4180 4911 493
Size: 711 1461 1515 731 2702
Fragments arranged by size:
2702 1515 1461 731 711
BsaBI GATnn'nnATC
Cuts at: 7114 7114
Size: 7120
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2066 2105 3789 3939
Size: 53 83 186 936 533 39 1684 150
Cuts at: 3939 3968 4117 4143 4259 4927 5309 5944 275
Size: 29 149 26 116 668 382 635 1451
Fragments arranged by size:
1684 1451 936 668 635 533 382 186
150 149 116 83 53 39 29 26
BsaWI w'CCGG_w
Cuts at: 2361 2699 5553 5951 6225 6372 6783 2361
Size: 338 2854 398 274 147 411 2698
Fragments arranged by size:
2854 2698 411 398 338 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2855 2885 2901 2931 2936
Size: 30 165 30 1452 30 16 30 5
Cuts at: 2936 2966 1178
Size: 30 5332
Fragments arranged by size:
5332 1452 165 30 30 30 30 30
16 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2072 2345 2348 2957 4412 1186
Size: 886 273 3 609 1455 3894
Fragments arranged by size:
3894 1455 886 609 273 3
BseYI C'CCAG_C
Cuts at: 2123 2382 3666 3670 4014 4050 6323 6819 2123
Size: 259 1284 4 344 36 2273 496 2424
Fragments arranged by size:
2424 2273 1284 496 344 259 36 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3387 3830 1163
Size: 573 1651 443 4453
Fragments arranged by size:
4453 1651 573 443
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2041 2889 4570 5331 6359 1540
Size: 275 186 40 848 1681 761 1028 2301
Fragments arranged by size:
2301 1681 1028 848 761 275 186 40
BsiHKAI G_wGCw'C
Cuts at: 2568 3765 3902 4205 4404 5181 5266 6337 2568
Size: 1197 137 303 199 777 85 1071 3351
Fragments arranged by size:
3351 1197 1071 777 303 199 137 85
BsmI GAATG_Cn'
Cuts at: 4631 4724 4631
Size: 93 7027
BsmBI CGTCTCn'nnnn_
Cuts at: 2020 2875 4134 2020
Size: 855 1259 5006
Fragments arranged by size:
5006 1259 855
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2568 2576 2914 3338 3525 3765 3840
Size: 399 778 8 338 424 187 240 75
Cuts at: 3840 3902 4205 4251 4294 4404 4439 5181 5266
Size: 62 303 46 43 110 35 742 85
Cuts at: 5266 6337 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 778 742 424 399 338 303
240 187 110 85 75 62 46 43
35 8
BspEI T'CCGG_A
Cuts at: 2361 6783 2361
Size: 4422 2698
BspHI T'CATG_A
Cuts at: 3711 3765 4224 5009 6739 3711
Size: 54 459 785 1730 4092
Fragments arranged by size:
4092 1730 785 459 54
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3232 3920 4475 844
Size: 2388 688 555 3489
Fragments arranged by size:
3489 2388 688 555
BsrBI CCG'CTC
Cuts at: 1394 1823 2338 4090 4195 5007 1394
Size: 429 515 1752 105 812 3507
Fragments arranged by size:
3507 1752 812 515 429 105
BsrDI GCAATG_nn'
Cuts at: 65 1248 5615 5789 65
Size: 1183 4367 174 1396
Fragments arranged by size:
4367 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2599 3228 3579 5764 5951 1700
Size: 110 789 629 351 2185 187 2869
Fragments arranged by size:
2869 2185 789 629 351 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7120
BssHII G'CGCG_C
Cuts at: 1925 2138 1925
Size: 213 6907
BssSI C'ACGA_G
Cuts at: 2420 2567 3122 5180 6192 2420
Size: 147 555 2058 1012 3348
Fragments arranged by size:
3348 2058 1012 555 147
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2882 3835 794
Size: 2088 953 4079
Fragments arranged by size:
4079 2088 953
BstBI TT'CG_AA
Cuts at: 3211 5924 3211
Size: 2713 4407
BstEII G'GTnAC_C
Cuts at: 1184 3862 4348 1184
Size: 2678 486 3956
Fragments arranged by size:
3956 2678 486
BstXI CCAn_nnnn'nTGG
Cuts at: 3151 3757 3781 3151
Size: 606 24 6490
Fragments arranged by size:
6490 606 24
BstYI r'GATC_y
Cuts at: 1972 2250 2406 2655 2784 2826 2982 3126 3591
Size: 278 156 249 129 42 156 144 465
Cuts at: 3591 4450 4817 5202 5219 6671 6757 6769 7115
Size: 859 367 385 17 1452 86 12 346
Cuts at: 7115 1972
Size: 1977
Fragments arranged by size:
1977 1452 859 465 385 367 346 278
249 156 156 144 129 86 42 17
12
Bsu36I CC'TnA_GG
Cuts at: 3917 4121 3917
Size: 204 6916
BtgI C'CryG_G
Cuts at: 513 1065 2004 2715 2733 2916 3040 3144 3387
Size: 552 939 711 18 183 124 104 243
Cuts at: 3387 3990 4206 4268 513
Size: 603 216 62 3365
Fragments arranged by size:
3365 939 711 603 552 243 216 183
124 104 62 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3124 487
Size: 683 56 1898 4483
Fragments arranged by size:
4483 1898 683 56
BtsI GCAGTG_nn'
Cuts at: 3180 3879 4636 5430 5450 3180
Size: 699 757 794 20 4850
Fragments arranged by size:
4850 794 757 699 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7120
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7085
DraI TTT'AAA
Cuts at: 4424 4424
Size: 7120
DraIII CAC_nnn'GTG
Cuts at: 2668 4078 4312 2668
Size: 1410 234 5476
Fragments arranged by size:
5476 1410 234
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6127 809
Size: 507 4811 1802
Fragments arranged by size:
4811 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2038 2730
Size: 54 1425 50 275 18 177 31 692
Cuts at: 2730 2886 3264 3276 4567 5456 7085 8
Size: 156 378 12 1291 889 1629 43
Fragments arranged by size:
1629 1425 1291 889 692 378 275 177
156 54 50 43 31 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2038 2886 4567 1537
Size: 275 226 848 1681 4090
Fragments arranged by size:
4090 1681 848 275 226
EarI CTCTTCn'nnn_
Cuts at: 1353 3413 3440 5050 1353
Size: 2060 27 1610 3423
Fragments arranged by size:
3423 2060 1610 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2356 2529 3644 5707 6081 6227
Size: 1064 138 767 173 1115 2063 374 146
Cuts at: 6227 387
Size: 1280
Fragments arranged by size:
2063 1280 1115 1064 767 374 173 146
138
EcoICRI GAG'CTC
Cuts at: 4402 4402
Size: 7120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2348 2998 4013 4148 5183 5780 6567
Size: 393 716 650 1015 135 1035 597 787
Cuts at: 6567 6835 1239
Size: 268 1524
Fragments arranged by size:
1524 1035 1015 787 716 650 597 393
268 135
EcoNI CCTnn'n_nnAGG
Cuts at: 2330 3916 2330
Size: 1586 5534
EcoO109I rG'GnC_Cy
Cuts at: 2725 2926 3055 3802 3856 4033 4291 4360 2725
Size: 201 129 747 54 177 258 69 5485
Fragments arranged by size:
5485 747 258 201 177 129 69 54
EcoRI G'AATT_C
Cuts at: 4429 4807 4429
Size: 378 6742
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2881 4408 2881
Size: 1527 5593
FspI TGC'GCA
Cuts at: 5626 5626
Size: 7120
HaeII r_GCGC'y
Cuts at: 1678 2069 2383 4120 6267 1678
Size: 391 314 1737 2147 2531
Fragments arranged by size:
2531 2147 1737 391 314
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2855 2887 2936 2968 3953
Size: 32 135 32 1283 32 49 32 985
Cuts at: 3953 3985 5761 5793 5835 5867 1373
Size: 32 1776 32 42 32 2626
Fragments arranged by size:
2626 1776 1283 985 135 49 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2802 4464 4710 669
Size: 988 281 864 1662 246 3079
Fragments arranged by size:
3079 1662 988 864 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7120
HpaI GTT'AAC
Cuts at: 4710 4710
Size: 7120
KasI G'GCGC_C
Cuts at: 2065 4116 2065
Size: 2051 5069
KpnI G_GTAC'C
Cuts at: 4375 4445 4375
Size: 70 7050
MfeI C'AATT_G
Cuts at: 4719 4719
Size: 7120
MluI A'CGCG_T
Cuts at: 6996 6996
Size: 7120
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2152 4119 6234 6418 1305
Size: 847 1967 2115 184 2007
Fragments arranged by size:
2115 2007 1967 847 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 5121
Fragments arranged by size:
5121 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2444 3143 3149 3713 3758 3905 4005
Size: 648 1278 699 6 564 45 147 100
Cuts at: 4005 4178 5078 5437 5596 518
Size: 173 900 359 159 2042
Fragments arranged by size:
2042 1278 900 699 648 564 359 173
159 147 100 45 6
MspA1I CmG'CkG
Cuts at: 1606 2071 2110 2320 2616 2900 3352 3670 3826
Size: 465 39 210 296 284 452 318 156
Cuts at: 3826 4114 4536 5213 6361 6606 1606
Size: 288 422 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 465 452 422 318 296
288 284 245 210 156 39
NaeI GCC'GGC
Cuts at: 3581 3581
Size: 7120
NarI GG'CG_CC
Cuts at: 2066 4117 2066
Size: 2051 5069
NcoI C'CATG_G
Cuts at: 513 1065 2004 3144 3387 4206 513
Size: 552 939 1140 243 819 3427
Fragments arranged by size:
3427 1140 939 819 552 243
NdeI CA'TA_TG
Cuts at: 387 2178 387
Size: 1791 5329
NgoMIV G'CCGG_C
Cuts at: 3579 3579
Size: 7120
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7120
NotI GC'GGCC_GC
Cuts at: 4567 4567
Size: 7120
NspI r_CATG'y
Cuts at: 1132 1229 3052 3817 4004 4478 6023 1132
Size: 97 1823 765 187 474 1545 2229
Fragments arranged by size:
2229 1823 1545 765 474 187 97
PasI CC'CwG_GG
Cuts at: 1348 4294 1348
Size: 2946 4174
PciI A'CATG_T
Cuts at: 3048 6019 3048
Size: 2971 4149
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7120
PfoI T'CCnGG_A
Cuts at: 2325 2325
Size: 7120
PmeI GTTT'AAAC
Cuts at: 4424 4424
Size: 7120
PmlI CAC'GTG
Cuts at: 2665 4180 2665
Size: 1515 5605
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5189 5221 6702 6734 5189
Size: 32 1481 32 5575
Fragments arranged by size:
5575 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2926 3055 4360 2926
Size: 129 1305 5686
Fragments arranged by size:
5686 1305 129
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7120
PsiI TTA'TAA
Cuts at: 4690 4690
Size: 7120
PspOMI G'GGCC_C
Cuts at: 4290 4290
Size: 7120
PstI C_TGCA'G
Cuts at: 830 3243 3330 3357 3831 4472 830
Size: 2413 87 27 474 641 3478
Fragments arranged by size:
3478 2413 641 474 87 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7120
PvuII CAG'CTG
Cuts at: 2320 3670 3826 2320
Size: 1350 156 5614
Fragments arranged by size:
5614 1350 156
SacI G_AGCT'C
Cuts at: 4404 4404
Size: 7120
SalI G'TCGA_C
Cuts at: 1936 4462 1936
Size: 2526 4594
SapI GCTCTTCn'nnn_
Cuts at: 3413 3413
Size: 7120
SbfI CC_TGCA'GG
Cuts at: 3243 3330 4472 3243
Size: 87 1142 5891
Fragments arranged by size:
5891 1142 87
ScaI AGT'ACT
Cuts at: 2837 5368 7080 2837
Size: 2531 1712 2877
Fragments arranged by size:
2877 2531 1712
SexAI A'CCwGG_T
Cuts at: 4308 4308
Size: 7120
SfcI C'TryA_G
Cuts at: 826 1045 3239 3326 3353 3827 4468 5603 6284
Size: 219 2194 87 27 474 641 1135 681
Cuts at: 6284 6475 826
Size: 191 1471
Fragments arranged by size:
2194 1471 1135 681 641 474 219 191
87 27
SfoI GGC'GCC
Cuts at: 2067 4118 2067
Size: 2051 5069
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7120
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7120
SmaI CCC'GGG
Cuts at: 1872 4447 1872
Size: 2575 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2279 2465 2675 5224 6125 6387
Size: 197 926 336 186 210 2549 901 262
Cuts at: 6387 820
Size: 1553
Fragments arranged by size:
2549 1553 926 901 336 262 210 197
186
SnaBI TAC'GTA
Cuts at: 493 4911 493
Size: 4418 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7120
SphI G_CATG'C
Cuts at: 1132 4004 4478 1132
Size: 2872 474 3774
Fragments arranged by size:
3774 2872 474
SspI AAT'ATT
Cuts at: 5 52 5044 5
Size: 47 4992 2081
Fragments arranged by size:
4992 2081 47
StuI AGG'CCT
Cuts at: 1517 3072 1517
Size: 1555 5565
StyI C'CwwG_G
Cuts at: 513 1065 2004 2244 3144 3387 4206 4559 513
Size: 552 939 240 900 243 819 353 3074
Fragments arranged by size:
3074 939 900 819 552 353 243 240
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2909 4028 4177 5147 5164 5317 5502
Size: 1255 1329 1119 149 970 17 153 185
Cuts at: 5502 5947 325
Size: 445 1498
Fragments arranged by size:
1498 1329 1255 1119 970 445 185 153
149 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2469 2835 3378
Size: 274 80 33 51 1443 492 366 543
Cuts at: 3378 5366 7078 96
Size: 1988 1712 138
Fragments arranged by size:
1988 1712 1443 543 492 366 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2223 2255 2901 2933 7080 7112 2223
Size: 32 646 32 4147 32 2231
Fragments arranged by size:
4147 2231 646 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2867 1316
Size: 1551 5569
XbaI T'CTAG_A
Cuts at: 4456 4456
Size: 7120
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3151 4102 4375 3151
Size: 951 273 5896
Fragments arranged by size:
5896 951 273
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7120
XmaI C'CCGG_G
Cuts at: 1870 4445 1870
Size: 2575 4545
XmnI GAAnn'nnTTC
Cuts at: 2966 4133 5249 2966
Size: 1167 1116 4837
Fragments arranged by size:
4837 1167 1116
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3790 4928 276
Size: 53 83 186 936 2256 1138 2468
Fragments arranged by size:
2468 2256 1138 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI
BstZ17I EcoRV FseI FspAI NruI NsiI PacI PspXI
PsrI RsrII SacII SanDI SfiI SrfI SwaI