Restriction map for
pFN21AE4578
(Circular) (Six-base) MAPSORT of: pFN21AE4578.seq Check: 3999 from: 1 to: 7027
pFN21AE4578 7027 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2882 2882
Size: 7027
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4837 278
Size: 53 83 186 936 3301 2468
Fragments arranged by size:
3301 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4370 1937
Size: 2433 4594
Acc65I G'GTAC_C
Cuts at: 4348 4348
Size: 7027
AclI AA'CG_TT
Cuts at: 1563 5154 5527 1563
Size: 3591 373 3063
Fragments arranged by size:
3591 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2908 4243 4276 5090 6474 6742 1632
Size: 1276 1335 33 814 1384 268 1917
Fragments arranged by size:
1917 1384 1335 1276 814 268 33
AfeI AGC'GCT
Cuts at: 2774 2774
Size: 7027
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6830
AflIII A'CryG_T
Cuts at: 1203 3858 4065 5926 6903 1203
Size: 2655 207 1861 977 1327
Fragments arranged by size:
2655 1861 1327 977 207
AgeI A'CCGG_T
Cuts at: 5858 5858
Size: 7027
AhdI GACnn_n'nnGTC
Cuts at: 3076 5756 3076
Size: 2680 4347
AleI CACnn'nnGTG
Cuts at: 1166 2603 3944 4109 4248 1166
Size: 1437 1341 165 139 3945
Fragments arranged by size:
3945 1437 1341 165 139
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7027
AlwNI CAG_nnn'CTG
Cuts at: 3134 3972 4770 6342 3134
Size: 838 798 1572 3819
Fragments arranged by size:
3819 1572 838 798
ApaI G_GGCC'C
Cuts at: 2364 2840 2364
Size: 476 6551
ApaLI G'TGCA_C
Cuts at: 3187 5084 6240 3187
Size: 1897 1156 3974
Fragments arranged by size:
3974 1897 1156
ApoI r'AATT_y
Cuts at: 1493 2504 2680 4336 4562 4714 6823 1493
Size: 1011 176 1656 226 152 2109 1697
Fragments arranged by size:
2109 1697 1656 1011 226 176 152
AseI AT'TA_AT
Cuts at: 160 1032 5581 160
Size: 872 4549 1606
Fragments arranged by size:
4549 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7027
AvaI C'yCGr_G
Cuts at: 1870 1943 2105 2941 3538 4352 1870
Size: 73 162 836 597 814 4545
Fragments arranged by size:
4545 836 814 597 162 73
BamHI G'GATC_C
Cuts at: 4357 4724 4357
Size: 367 6660
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2246 2996 3938 4348 5803
Size: 325 217 627 459 750 942 410 1455
Cuts at: 5803 618
Size: 1842
Fragments arranged by size:
1842 1455 942 750 627 459 410 325
217
BanII G_rGCy'C
Cuts at: 1391 2364 2840 4302 4346 1391
Size: 973 476 1462 44 4072
Fragments arranged by size:
4072 1462 973 476 44
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2914 928
Size: 971 1015 5041
Fragments arranged by size:
5041 1015 971
BbvCI CC'TCA_GC
Cuts at: 3751 3751
Size: 7027
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3242 3276 5218 5252 1903
Size: 34 1305 34 1942 34 3678
Fragments arranged by size:
3678 1942 1305 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4919 6129 4919
Size: 1210 5817
BclI T'GATC_A
Cuts at: 1548 2520 1548
Size: 972 6055
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2162 2244 3125
Size: 107 122 71 631 605 490 82 881
Cuts at: 3125 3318 3444 3600 5638 136
Size: 193 126 156 2038 1525
Fragments arranged by size:
2038 1525 881 631 605 490 193 156
126 122 107 82 71
BglII A'GATC_T
Cuts at: 2652 7022 2652
Size: 4370 2657
BlpI GC'TnA_GC
Cuts at: 4444 4444
Size: 7027
Bme1580I G_kGCm'C
Cuts at: 1790 2249 2364 2840 3191 4116 5088 6244 1790
Size: 459 115 476 351 925 972 1156 2573
Fragments arranged by size:
2573 1156 972 925 476 459 351 115
BmgBI CAC'GTC
Cuts at: 1318 2287 3586 4111 1318
Size: 969 1299 525 4234
Fragments arranged by size:
4234 1299 969 525
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2597 3155 3415
Size: 416 53 83 399 24 1180 558 260
Cuts at: 3415 4758 5716 442
Size: 1343 958 1753
Fragments arranged by size:
1753 1343 1180 958 558 416 399 260
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7027
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2594 2594
Size: 7027
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2806 3713 4140 5687 1239
Size: 1567 907 427 1547 2579
Fragments arranged by size:
2579 1567 1547 907 427
Bpu10I CC'TnA_GC
Cuts at: 2069 3751 2069
Size: 1682 5345
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5152 6017 6315 5152
Size: 865 298 5864
Fragments arranged by size:
5864 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3470 5690 882
Size: 617 1971 2220 2219
Fragments arranged by size:
2220 2219 1971 617
BsaAI yAC'GTr
Cuts at: 493 1204 2311 2620 4818 493
Size: 711 1107 309 2198 2702
Fragments arranged by size:
2702 2198 1107 711 309
BsaBI GATnn'nnATC
Cuts at: 7021 7021
Size: 7027
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2436 4834 5216 5851
Size: 53 83 186 936 903 2398 382 635
Cuts at: 5851 275
Size: 1451
Fragments arranged by size:
2398 1451 936 903 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2949 5460 5858 6132 6279 6690 2949
Size: 2511 398 274 147 411 3286
Fragments arranged by size:
3286 2511 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2252 2282 2306 2336 3467
Size: 30 165 30 849 30 24 30 1131
Cuts at: 3467 3497 1178
Size: 30 4708
Fragments arranged by size:
4708 1131 849 165 30 30 30 30
30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2329 3269 3746 3793 1186
Size: 1143 940 477 47 4420
Fragments arranged by size:
4420 1143 940 477 47
BseYI C'CCAG_C
Cuts at: 2186 2302 2430 2833 3103 3442 4059 6230 6726
Size: 116 128 403 270 339 617 2171 496
Cuts at: 6726 2186
Size: 2487
Fragments arranged by size:
2487 2171 617 496 403 339 270 128
116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2497 2782 3629 3695 1163
Size: 573 761 285 847 66 4495
Fragments arranged by size:
4495 847 761 573 285 66
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2895 3325 3853 4477 5238 6266
Size: 275 186 894 430 528 624 761 1028
Cuts at: 6266 1540
Size: 2301
Fragments arranged by size:
2301 1028 894 761 624 528 430 275
186
BsiHKAI G_wGCw'C
Cuts at: 2024 3030 3191 3539 4290 5088 5173 6244 2024
Size: 1006 161 348 751 798 85 1071 2807
Fragments arranged by size:
2807 1071 1006 798 751 348 161 85
BsmI GAATG_Cn'
Cuts at: 4538 4631 4538
Size: 93 6934
BsmBI CGTCTCn'nnnn_
Cuts at: 4262 4262
Size: 7027
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2249 2364 2840 3030 3191 3539
Size: 399 234 225 115 476 190 161 348
Cuts at: 3539 4116 4290 4302 4346 5088 5173 6244 1391
Size: 577 174 12 44 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 577 476 399 348 234
225 190 174 161 115 85 44 12
BspEI T'CCGG_A
Cuts at: 2949 6690 2949
Size: 3741 3286
BspHI T'CATG_A
Cuts at: 3519 4191 4916 6646 3519
Size: 672 725 1730 3900
Fragments arranged by size:
3900 1730 725 672
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2882 2908 3930 4315 4382 844
Size: 2038 26 1022 385 67 3489
Fragments arranged by size:
3489 2038 1022 385 67 26
BsrBI CCG'CTC
Cuts at: 1394 1823 4914 1394
Size: 429 3091 3507
Fragments arranged by size:
3507 3091 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3202 5522 5696 65
Size: 1183 1954 2320 174 1396
Fragments arranged by size:
2320 1954 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2122 2801 2890 3699 3786 5671 5858
Size: 110 312 679 89 809 87 1885 187
Cuts at: 5858 1700
Size: 2869
Fragments arranged by size:
2869 1885 809 679 312 187 110 89
87
BsrGI T'GTAC_A
Cuts at: 96 2742 4233 96
Size: 2646 1491 2890
Fragments arranged by size:
2890 2646 1491
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7027
BssSI C'ACGA_G
Cuts at: 3251 5087 6099 3251
Size: 1836 1012 4179
Fragments arranged by size:
4179 1836 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2253 794
Size: 1459 5568
BstBI TT'CG_AA
Cuts at: 5831 5831
Size: 7027
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7027
BstXI CCAn_nnnn'nTGG
Cuts at: 2390 3691 3821 4250 2390
Size: 1301 130 429 5167
Fragments arranged by size:
5167 1301 429 130
BstYI r'GATC_y
Cuts at: 1972 2652 3180 3810 3864 4357 4724 5109 5126
Size: 680 528 630 54 493 367 385 17
Cuts at: 5126 6578 6664 6676 7022 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 680 630 528 493 385 367
346 86 54 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2790 2895 3288 3423 3726 3774
Size: 552 939 786 105 393 135 303 48
Cuts at: 3774 4050 513
Size: 276 3490
Fragments arranged by size:
3490 939 786 552 393 303 276 135
105 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2271 487
Size: 683 56 1045 5243
Fragments arranged by size:
5243 1045 683 56
BtsI GCAGTG_nn'
Cuts at: 3562 3725 4543 5337 5357 3562
Size: 163 818 794 20 5232
Fragments arranged by size:
5232 818 794 163 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7027
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6992
DraI TTT'AAA
Cuts at: 4331 4331
Size: 7027
DraIII CAC_nnn'GTG
Cuts at: 2253 2253
Size: 7027
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6034 809
Size: 507 4718 1802
Fragments arranged by size:
4718 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2163 2236 2673
Size: 54 1425 50 275 18 333 73 437
Cuts at: 2673 2780 2892 3081 3322 3397 3823 3850 4216
Size: 107 112 189 241 75 426 27 366
Cuts at: 4216 4474 5363 6992 8
Size: 258 889 1629 43
Fragments arranged by size:
1629 1425 889 437 426 366 333 275
258 241 189 112 107 75 73 54
50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 2892 3322 3850 4474 1537
Size: 275 1080 430 528 624 4090
Fragments arranged by size:
4090 1080 624 528 430 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2189 4260 4957 1353
Size: 836 2071 697 3423
Fragments arranged by size:
3423 2071 836 697
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3618 5614 5988 6134 387
Size: 1064 138 2029 1996 374 146 1280
Fragments arranged by size:
2029 1996 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2806 2908 3713 4140 4243 4276 5090
Size: 393 1174 102 805 427 103 33 814
Cuts at: 5090 5687 6474 6742 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1174 814 805 787 597 427 393
268 103 102 33
EcoNI CCTnn'n_nnAGG
Cuts at: 2761 4133 2761
Size: 1372 5655
EcoO109I rG'GnC_Cy
Cuts at: 2149 2360 2837 3070 3372 2149
Size: 211 477 233 302 5804
Fragments arranged by size:
5804 477 302 233 211
EcoRI G'AATT_C
Cuts at: 2680 4336 4714 2680
Size: 1656 378 4993
Fragments arranged by size:
4993 1656 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3985 3985
Size: 7027
FspI TGC'GCA
Cuts at: 5533 5533
Size: 7027
HaeII r_GCGC'y
Cuts at: 1678 2776 6174 1678
Size: 1098 3398 2531
Fragments arranged by size:
3398 2531 1098
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2252 2284 2306 2338 2924
Size: 32 135 32 680 32 22 32 586
Cuts at: 2924 2956 3062 3094 5668 5700 5742 5774 1373
Size: 32 106 32 2574 32 42 32 2626
Fragments arranged by size:
2626 2574 680 586 135 106 42 32
32 32 32 32 32 32 32 22
HincII GTy'rAC
Cuts at: 669 1657 1938 3502 4099 4371 4617 669
Size: 988 281 1564 597 272 246 3079
Fragments arranged by size:
3079 1564 988 597 281 272 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7027
HpaI GTT'AAC
Cuts at: 4617 4617
Size: 7027
KpnI G_GTAC'C
Cuts at: 4352 4352
Size: 7027
MfeI C'AATT_G
Cuts at: 4626 4626
Size: 7027
MluI A'CGCG_T
Cuts at: 3858 6903 3858
Size: 3045 3982
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3855 4206 6141 6325 1305
Size: 2550 351 1935 184 2007
Fragments arranged by size:
2550 2007 1935 351 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2165 2238 2782 3399 3825
Size: 54 1425 343 333 73 544 617 426
Cuts at: 3825 10
Size: 3212
Fragments arranged by size:
3212 1425 617 544 426 343 333 73
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2603 3495 3819 3902 3944 4049 4109
Size: 648 1437 892 324 83 42 105 60
Cuts at: 4109 4229 4248 4985 5344 5503 518
Size: 120 19 737 359 159 2042
Fragments arranged by size:
2042 1437 892 737 648 359 324 159
120 105 83 60 42 19
MspA1I CmG'CkG
Cuts at: 1606 2037 2091 2176 2826 3442 4296 4315 4443
Size: 431 54 85 650 616 854 19 128
Cuts at: 4443 5120 6268 6513 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 854 677 650 616 431 245
128 85 54 19
NaeI GCC'GGC
Cuts at: 3788 3788
Size: 7027
NcoI C'CATG_G
Cuts at: 513 1065 2004 2790 3288 3423 3726 4050 513
Size: 552 939 786 498 135 303 324 3490
Fragments arranged by size:
3490 939 786 552 498 324 303 135
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7027
NgoMIV G'CCGG_C
Cuts at: 3786 3786
Size: 7027
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7027
NotI GC'GGCC_GC
Cuts at: 3322 4474 3322
Size: 1152 5875
NspI r_CATG'y
Cuts at: 1132 1229 2418 2704 4069 4385 5930 1132
Size: 97 1189 286 1365 316 1545 2229
Fragments arranged by size:
2229 1545 1365 1189 316 286 97
PasI CC'CwG_GG
Cuts at: 1348 2714 3598 1348
Size: 1366 884 4777
Fragments arranged by size:
4777 1366 884
PciI A'CATG_T
Cuts at: 4065 5926 4065
Size: 1861 5166
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2325 2430 3898 1108
Size: 1217 105 1468 4237
Fragments arranged by size:
4237 1468 1217 105
PfoI T'CCnGG_A
Cuts at: 2324 3471 2324
Size: 1147 5880
PmeI GTTT'AAAC
Cuts at: 4331 4331
Size: 7027
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3728 3760 5096 5128 6609 6641 3728
Size: 32 1336 32 1481 32 4114
Fragments arranged by size:
4114 1481 1336 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3070 3372 3070
Size: 302 6725
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7027
PsiI TTA'TAA
Cuts at: 4597 4597
Size: 7027
PspOMI G'GGCC_C
Cuts at: 2360 2836 2360
Size: 476 6551
PstI C_TGCA'G
Cuts at: 830 2036 3098 3720 4379 830
Size: 1206 1062 622 659 3478
Fragments arranged by size:
3478 1206 1062 659 622
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7027
PvuII CAG'CTG
Cuts at: 2037 2091 2176 2826 3442 4315 2037
Size: 54 85 650 616 873 4749
Fragments arranged by size:
4749 873 650 616 85 54
SalI G'TCGA_C
Cuts at: 1936 4369 1936
Size: 2433 4594
SanDI GG'GwC_CC
Cuts at: 3372 3372
Size: 7027
SbfI CC_TGCA'GG
Cuts at: 4379 4379
Size: 7027
ScaI AGT'ACT
Cuts at: 2111 5275 6987 2111
Size: 3164 1712 2151
Fragments arranged by size:
3164 2151 1712
SexAI A'CCwGG_T
Cuts at: 3072 3479 3651 3072
Size: 407 172 6448
Fragments arranged by size:
6448 407 172
SfcI C'TryA_G
Cuts at: 826 1045 2032 3094 3716 4375 5510 6191 6382
Size: 219 987 1062 622 659 1135 681 191
Cuts at: 6382 826
Size: 1471
Fragments arranged by size:
1471 1135 1062 987 681 659 622 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7027
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7027
SmaI CCC'GGG
Cuts at: 1872 4354 1872
Size: 2482 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2941 5131 6032 6294 820
Size: 197 926 998 2190 901 262 1553
Fragments arranged by size:
2190 1553 998 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4818 493
Size: 4325 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7027
SphI G_CATG'C
Cuts at: 1132 2418 4385 1132
Size: 1286 1967 3774
Fragments arranged by size:
3774 1967 1286
SspI AAT'ATT
Cuts at: 5 52 4951 5
Size: 47 4899 2081
Fragments arranged by size:
4899 2081 47
StuI AGG'CCT
Cuts at: 1517 2014 1517
Size: 497 6530
StyI C'CwwG_G
Cuts at: 513 1065 2004 2790 3288 3423 3726 3942 4050
Size: 552 939 786 498 135 303 216 108
Cuts at: 4050 4466 513
Size: 416 3074
Fragments arranged by size:
3074 939 786 552 498 416 303 216
135 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3779 3926 5054 5071 5224 5409 5854
Size: 1255 2199 147 1128 17 153 185 445
Cuts at: 5854 325
Size: 1498
Fragments arranged by size:
2199 1498 1255 1128 445 185 153 147
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2109 2226 2387
Size: 274 80 33 51 1443 132 117 161
Cuts at: 2387 2742 4233 5273 6985 96
Size: 355 1491 1040 1712 138
Fragments arranged by size:
1712 1491 1443 1040 355 274 161 138
132 117 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3379 3411 6987 7019 3379
Size: 32 3576 32 3387
Fragments arranged by size:
3576 3387 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3653 1316
Size: 2337 4690
XbaI T'CTAG_A
Cuts at: 4363 4363
Size: 7027
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2830 3973 2830
Size: 1143 5884
XhoI C'TCGA_G
Cuts at: 1943 2941 1943
Size: 998 6029
XmaI C'CCGG_G
Cuts at: 1870 4352 1870
Size: 2482 4545
XmnI GAAnn'nnTTC
Cuts at: 5156 5156
Size: 7027
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4835 276
Size: 53 83 186 936 3301 2468
Fragments arranged by size:
3301 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I
Bsu36I EcoICRI EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspXI PsrI RsrII SacI SacII
SapI SfiI SfoI SrfI SwaI