Restriction map for
pFN21AE4577
(Circular) (Six-base) MAPSORT of: pFN21AE4577.seq Check: 4698 from: 1 to: 6997
pFN21AE4577 6997 bp
With 182 enzymes: SgfI *
December 3, 2014 10:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4046 4178 4046
Size: 132 6865
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4807 278
Size: 53 83 186 936 3271 2468
Fragments arranged by size:
3271 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4340 1937
Size: 2403 4594
Acc65I G'GTAC_C
Cuts at: 3486 4318 3486
Size: 832 6165
AclI AA'CG_TT
Cuts at: 1563 4296 5124 5497 1563
Size: 2733 828 373 3063
Fragments arranged by size:
3063 2733 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2918 3269 4099 5060 6444 6712 1632
Size: 1286 351 830 961 1384 268 1917
Fragments arranged by size:
1917 1384 1286 961 830 351 268
AfeI AGC'GCT
Cuts at: 2500 4106 2500
Size: 1606 5391
AflII C'TTAA_G
Cuts at: 820 1017 2384 820
Size: 197 1367 5433
Fragments arranged by size:
5433 1367 197
AflIII A'CryG_T
Cuts at: 1203 3207 3378 3545 5896 6873 1203
Size: 2004 171 167 2351 977 1327
Fragments arranged by size:
2351 2004 1327 977 171 167
AgeI A'CCGG_T
Cuts at: 5828 5828
Size: 6997
AhdI GACnn_n'nnGTC
Cuts at: 5726 5726
Size: 6997
AleI CACnn'nnGTG
Cuts at: 1166 3449 1166
Size: 2283 4714
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3887 1184
Size: 2703 4294
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2114 2146 2114
Size: 32 6965
AlwNI CAG_nnn'CTG
Cuts at: 2455 3439 3463 4156 4740 6312 2455
Size: 984 24 693 584 1572 3140
Fragments arranged by size:
3140 1572 984 693 584 24
ApaI G_GGCC'C
Cuts at: 2839 3769 2839
Size: 930 6067
ApaLI G'TGCA_C
Cuts at: 2470 5054 6210 2470
Size: 2584 1156 3257
Fragments arranged by size:
3257 2584 1156
ApoI r'AATT_y
Cuts at: 1493 2150 3809 4306 4532 4684 6793 1493
Size: 657 1659 497 226 152 2109 1697
Fragments arranged by size:
2109 1697 1659 657 497 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5551 160
Size: 872 4519 1606
Fragments arranged by size:
4519 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6997
AvaI C'yCGr_G
Cuts at: 1870 1943 2256 2267 2577 4205 4322 1870
Size: 73 313 11 310 1628 117 4545
Fragments arranged by size:
4545 1628 313 310 117 73 11
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3763 3796 3763
Size: 33 6964
BamHI G'GATC_C
Cuts at: 2212 4327 4694 2212
Size: 2115 367 4515
Fragments arranged by size:
4515 2115 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2198 3372 3486 3687 4250
Size: 325 217 627 411 1174 114 201 563
Cuts at: 4250 4318 5773 618
Size: 68 1455 1842
Fragments arranged by size:
1842 1455 1174 627 563 411 325 217
201 114 68
BanII G_rGCy'C
Cuts at: 1391 2345 2823 2839 3098 3129 3769 3804 4316
Size: 954 478 16 259 31 640 35 512
Cuts at: 4316 1391
Size: 4072
Fragments arranged by size:
4072 954 640 512 478 259 35 31
16
BbeI G_GCGC'C
Cuts at: 3376 3691 3376
Size: 315 6682
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3628 928
Size: 971 1729 4297
Fragments arranged by size:
4297 1729 971
BbvCI CC'TCA_GC
Cuts at: 2448 2448
Size: 6997
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5188 5222 1903
Size: 34 3251 34 3678
Fragments arranged by size:
3678 3251 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3791 4889 6099 3791
Size: 1098 1210 4689
Fragments arranged by size:
4689 1210 1098
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6997
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5608 136
Size: 107 122 71 631 605 3936 1525
Fragments arranged by size:
3936 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6992 6992
Size: 6997
BlpI GC'TnA_GC
Cuts at: 4414 4414
Size: 6997
Bme1580I G_kGCm'C
Cuts at: 1790 2474 2839 3769 4275 5058 6214 1790
Size: 684 365 930 506 783 1156 2573
Fragments arranged by size:
2573 1156 930 783 684 506 365
BmgBI CAC'GTC
Cuts at: 1318 2358 1318
Size: 1040 5957
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2461 3763 4144
Size: 416 53 83 399 24 1044 1302 381
Cuts at: 4144 4728 5686 442
Size: 584 958 1753
Fragments arranged by size:
1753 1302 1044 958 584 416 399 381
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6997
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2192 2255 3076 2192
Size: 63 821 6113
Fragments arranged by size:
6113 821 63
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2002 2010 2711 3089 3994 4031 4271 5657
Size: 763 8 701 378 905 37 240 1386
Cuts at: 5657 1239
Size: 2579
Fragments arranged by size:
2579 1386 905 763 701 378 240 37
8
Bpu10I CC'TnA_GC
Cuts at: 2297 2448 3671 2297
Size: 151 1223 5623
Fragments arranged by size:
5623 1223 151
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2882 5122 5987 6285 2882
Size: 2240 865 298 3594
Fragments arranged by size:
3594 2240 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2257 3938 5660 882
Size: 617 758 1681 1722 2219
Fragments arranged by size:
2219 1722 1681 758 617
BsaAI yAC'GTr
Cuts at: 493 1204 3379 3548 3892 4788 493
Size: 711 2175 169 344 896 2702
Fragments arranged by size:
2702 2175 896 711 344 169
BsaBI GATnn'nnATC
Cuts at: 6991 6991
Size: 6997
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3373 3688 4804 5186
Size: 53 83 186 936 1840 315 1116 382
Cuts at: 5186 5821 275
Size: 635 1451
Fragments arranged by size:
1840 1451 1116 936 635 382 315 186
83 53
BsaWI w'CCGG_w
Cuts at: 2145 5430 5828 6102 6249 6660 2145
Size: 3285 398 274 147 411 2482
Fragments arranged by size:
3285 2482 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2114 2144 1178
Size: 30 165 30 711 30 6031
Fragments arranged by size:
6031 711 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2539 2813 3068 3332 4139 4274 1186
Size: 1353 274 255 264 807 135 3909
Fragments arranged by size:
3909 1353 807 274 264 255 135
BseYI C'CCAG_C
Cuts at: 2831 3517 6200 6696 2831
Size: 686 2683 496 3132
Fragments arranged by size:
3132 2683 686 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2489 2876 3749 3836 4057 1163
Size: 573 753 387 873 87 221 4103
Fragments arranged by size:
4103 873 753 573 387 221 87
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2590 4447 5208 6236 1540
Size: 275 186 589 1857 761 1028 2301
Fragments arranged by size:
2301 1857 1028 761 589 275 186
BsiHKAI G_wGCw'C
Cuts at: 2474 3804 5058 5143 6214 2474
Size: 1330 1254 85 1071 3257
Fragments arranged by size:
3257 1330 1254 1071 85
BsmI GAATG_Cn'
Cuts at: 2406 4508 4601 2406
Size: 2102 93 4802
Fragments arranged by size:
4802 2102 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2345 2474 2823 2839 3098 3129 3769
Size: 399 555 129 349 16 259 31 640
Cuts at: 3769 3804 4275 4316 5058 5143 6214 1391
Size: 35 471 41 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 640 555 471 399 349
259 129 85 41 35 31 16
BspEI T'CCGG_A
Cuts at: 6660 6660
Size: 6997
BspHI T'CATG_A
Cuts at: 3579 3936 4886 6616 3579
Size: 357 950 1730 3960
Fragments arranged by size:
3960 1730 950 357
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2886 3498 3540 3615 4046 4178 4352 844
Size: 2042 612 42 75 431 132 174 3489
Fragments arranged by size:
3489 2042 612 431 174 132 75 42
BsrBI CCG'CTC
Cuts at: 1394 1823 2106 4884 1394
Size: 429 283 2778 3507
Fragments arranged by size:
3507 2778 429 283
BsrDI GCAATG_nn'
Cuts at: 65 1248 2790 3075 3085 5492 5666 65
Size: 1183 1542 285 10 2407 174 1396
Fragments arranged by size:
2407 1542 1396 1183 285 174 10
BsrFI r'CCGG_y
Cuts at: 1700 1810 5641 5828 1700
Size: 110 3831 187 2869
Fragments arranged by size:
3831 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6997
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6997
BssSI C'ACGA_G
Cuts at: 3344 5057 6069 3344
Size: 1713 1012 4272
Fragments arranged by size:
4272 1713 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2944 4156 794
Size: 2150 1212 3635
Fragments arranged by size:
3635 2150 1212
BstBI TT'CG_AA
Cuts at: 5801 5801
Size: 6997
BstEII G'GTnAC_C
Cuts at: 1184 2800 1184
Size: 1616 5381
BstXI CCAn_nnnn'nTGG
Cuts at: 2066 3823 2066
Size: 1757 5240
BstYI r'GATC_y
Cuts at: 1972 2212 2272 2884 3157 3333 3390 4327 4694
Size: 240 60 612 273 176 57 937 367
Cuts at: 4694 5079 5096 6548 6634 6646 6992 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 937 612 385 367 346 273
240 176 86 60 57 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2118 3735 3859 513
Size: 552 1053 1617 124 3651
Fragments arranged by size:
3651 1617 1053 552 124
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2494 3913 487
Size: 683 56 1268 1419 3571
Fragments arranged by size:
3571 1419 1268 683 56
BtsI GCAGTG_nn'
Cuts at: 2414 2540 2672 4513 5307 5327 2414
Size: 126 132 1841 794 20 4084
Fragments arranged by size:
4084 1841 794 132 126 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6997
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6962
DraI TTT'AAA
Cuts at: 4301 4301
Size: 6997
DraIII CAC_nnn'GTG
Cuts at: 2994 2994
Size: 6997
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6004 809
Size: 507 4688 1802
Fragments arranged by size:
4688 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2587 2649 2904
Size: 54 1425 50 275 18 757 62 255
Cuts at: 2904 3474 4444 5333 6962 8
Size: 570 970 889 1629 43
Fragments arranged by size:
1629 1425 970 889 757 570 275 255
62 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2587 4444 1537
Size: 275 775 1857 4090
Fragments arranged by size:
4090 1857 775 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2246 2516 3428 3809 4927 1353
Size: 893 270 912 381 1118 3423
Fragments arranged by size:
3423 1118 912 893 381 270
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2093 5584 5958 6104 387
Size: 1064 138 504 3491 374 146 1280
Fragments arranged by size:
3491 1280 1064 504 374 146 138
EcoICRI GAG'CTC
Cuts at: 3802 3802
Size: 6997
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2002 2010 2711 2918 3089 3269 3994
Size: 393 370 8 701 207 171 180 725
Cuts at: 3994 4031 4099 4271 5060 5657 6444 6712 1239
Size: 37 68 172 789 597 787 268 1524
Fragments arranged by size:
1524 789 787 725 701 597 393 370
268 207 180 172 171 68 37 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2366 3431 3674 2366
Size: 1065 243 5689
Fragments arranged by size:
5689 1065 243
EcoO109I rG'GnC_Cy
Cuts at: 2835 3436 3604 3707 3765 4174 2835
Size: 601 168 103 58 409 5658
Fragments arranged by size:
5658 601 409 168 103 58
EcoRI G'AATT_C
Cuts at: 4306 4684 4306
Size: 378 6619
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3196 3196
Size: 6997
FspI TGC'GCA
Cuts at: 5503 5503
Size: 6997
HaeII r_GCGC'y
Cuts at: 1678 2502 3376 3691 4108 6144 1678
Size: 824 874 315 417 2036 2531
Fragments arranged by size:
2531 2036 874 824 417 315
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2420 2452 4163 4195 4238
Size: 32 135 32 848 32 1711 32 43
Cuts at: 4238 4270 5638 5670 5712 5744 1373
Size: 32 1368 32 42 32 2626
Fragments arranged by size:
2626 1711 1368 848 135 43 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2173 3952 4341 4587 669
Size: 988 281 235 1779 389 246 3079
Fragments arranged by size:
3079 1779 988 389 281 246 235
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6997
HpaI GTT'AAC
Cuts at: 4587 4587
Size: 6997
KasI G'GCGC_C
Cuts at: 3372 3687 3372
Size: 315 6682
KpnI G_GTAC'C
Cuts at: 3490 4322 3490
Size: 832 6165
MfeI C'AATT_G
Cuts at: 4596 4596
Size: 6997
MluI A'CGCG_T
Cuts at: 6873 6873
Size: 6997
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2019 3265 4317 6111 6295 1305
Size: 714 1246 1052 1794 184 2007
Fragments arranged by size:
2007 1794 1246 1052 714 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3476 10
Size: 54 1425 343 1644 3531
Fragments arranged by size:
3531 1644 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3449 3821 4955 5314 5473 518
Size: 648 2283 372 1134 359 159 2042
Fragments arranged by size:
2283 2042 1134 648 372 359 159
MspA1I CmG'CkG
Cuts at: 1606 2783 2950 3048 3224 3355 3850 3861 3972
Size: 1177 167 98 176 131 495 11 111
Cuts at: 3972 4051 4413 5090 6238 6483 1606
Size: 79 362 677 1148 245 2120
Fragments arranged by size:
2120 1177 1148 677 495 362 245 176
167 131 111 98 79 11
NarI GG'CG_CC
Cuts at: 3373 3688 3373
Size: 315 6682
NcoI C'CATG_G
Cuts at: 513 1065 2118 3735 513
Size: 552 1053 1617 3775
Fragments arranged by size:
3775 1617 1053 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6997
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6997
NotI GC'GGCC_GC
Cuts at: 2587 4444 2587
Size: 1857 5140
NspI r_CATG'y
Cuts at: 1132 1229 2576 2756 2812 3211 3925 4355 5900
Size: 97 1347 180 56 399 714 430 1545
Cuts at: 5900 1132
Size: 2229
Fragments arranged by size:
2229 1545 1347 714 430 399 180 97
56
PasI CC'CwG_GG
Cuts at: 1348 2734 1348
Size: 1386 5611
PciI A'CATG_T
Cuts at: 3207 5896 3207
Size: 2689 4308
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2667 3483 1108
Size: 1559 816 4622
Fragments arranged by size:
4622 1559 816
PfoI T'CCnGG_A
Cuts at: 2688 3783 3840 4008 2688
Size: 1095 57 168 5677
Fragments arranged by size:
5677 1095 168 57
PmeI GTTT'AAAC
Cuts at: 4301 4301
Size: 6997
PmlI CAC'GTG
Cuts at: 3379 3548 3379
Size: 169 6828
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2114 2146 5066 5098 6579 6611 2114
Size: 32 2920 32 1481 32 2500
Fragments arranged by size:
2920 2500 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3604 4174 3604
Size: 570 6427
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6997
PsiI TTA'TAA
Cuts at: 4567 4567
Size: 6997
PspOMI G'GGCC_C
Cuts at: 2835 3765 2835
Size: 930 6067
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3521 3553 3521
Size: 32 6965
PstI C_TGCA'G
Cuts at: 830 2424 2535 2721 2883 2949 3147 3993 4349
Size: 1594 111 186 162 66 198 846 356
Cuts at: 4349 830
Size: 3478
Fragments arranged by size:
3478 1594 846 356 198 186 162 111
66
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6997
PvuII CAG'CTG
Cuts at: 2783 3048 3355 3850 3972 2783
Size: 265 307 495 122 5808
Fragments arranged by size:
5808 495 307 265 122
SacI G_AGCT'C
Cuts at: 3804 3804
Size: 6997
SacII CC_GC'GG
Cuts at: 3862 3862
Size: 6997
SalI G'TCGA_C
Cuts at: 1936 4339 1936
Size: 2403 4594
SapI GCTCTTCn'nnn_
Cuts at: 2246 3809 2246
Size: 1563 5434
SbfI CC_TGCA'GG
Cuts at: 2721 2883 4349 2721
Size: 162 1466 5369
Fragments arranged by size:
5369 1466 162
ScaI AGT'ACT
Cuts at: 5245 6957 5245
Size: 1712 5285
SfcI C'TryA_G
Cuts at: 826 1045 2420 2531 2717 2879 2945 3143 3571
Size: 219 1375 111 186 162 66 198 428
Cuts at: 3571 3654 3702 3989 4345 5480 6161 6352 826
Size: 83 48 287 356 1135 681 191 1471
Fragments arranged by size:
1471 1375 1135 681 428 356 287 219
198 191 186 162 111 83 66 48
SfoI GGC'GCC
Cuts at: 3374 3689 3374
Size: 315 6682
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6997
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6997
SmaI CCC'GGG
Cuts at: 1872 2258 2579 4324 1872
Size: 386 321 1745 4545
Fragments arranged by size:
4545 1745 386 321
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2384 2861 4205 5101 6002 6264
Size: 197 926 441 477 1344 896 901 262
Cuts at: 6264 820
Size: 1553
Fragments arranged by size:
1553 1344 926 901 896 477 441 262
197
SnaBI TAC'GTA
Cuts at: 493 4788 493
Size: 4295 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6997
SphI G_CATG'C
Cuts at: 1132 2576 2756 4355 1132
Size: 1444 180 1599 3774
Fragments arranged by size:
3774 1599 1444 180
SrfI GCCC'GGGC
Cuts at: 2579 2579
Size: 6997
SspI AAT'ATT
Cuts at: 5 52 4921 5
Size: 47 4869 2081
Fragments arranged by size:
4869 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6997
StyI C'CwwG_G
Cuts at: 513 1065 2118 2608 2910 3735 3795 4233 4436
Size: 552 1053 490 302 825 60 438 203
Cuts at: 4436 513
Size: 3074
Fragments arranged by size:
3074 1053 825 552 490 438 302 203
60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2244 5024 5041 5194 5379 5824 325
Size: 1255 664 2780 17 153 185 445 1498
Fragments arranged by size:
2780 1498 1255 664 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3771 5243 6955
Size: 274 80 33 51 1443 1794 1472 1712
Cuts at: 6955 96
Size: 138
Fragments arranged by size:
1794 1712 1472 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3537 3569 6957 6989 3537
Size: 32 3388 32 3545
Fragments arranged by size:
3545 3388 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2393 1316
Size: 1077 5920
XbaI T'CTAG_A
Cuts at: 4333 4333
Size: 6997
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4006 4006
Size: 6997
XhoI C'TCGA_G
Cuts at: 1943 4205 1943
Size: 2262 4735
XmaI C'CCGG_G
Cuts at: 1870 2256 2577 4322 1870
Size: 386 321 1745 4545
Fragments arranged by size:
4545 1745 386 321
XmnI GAAnn'nnTTC
Cuts at: 5126 5126
Size: 6997
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4805 276
Size: 53 83 186 936 3271 2468
Fragments arranged by size:
3271 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI
RsrII SanDI SexAI SfiI SwaI