Restriction map for
pFN21AE4575
(Circular) (Six-base) MAPSORT of: pFN21AE4575.seq Check: 3774 from: 1 to: 6979
pFN21AE4575 6979 bp
With 182 enzymes: SgfI *
July 6, 2012 17:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4789 278
Size: 53 83 186 936 3253 2468
Fragments arranged by size:
3253 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2358 3773 4322 1937
Size: 421 1415 549 4594
Fragments arranged by size:
4594 1415 549 421
Acc65I G'GTAC_C
Cuts at: 4300 4300
Size: 6979
AclI AA'CG_TT
Cuts at: 1563 5106 5479 1563
Size: 3543 373 3063
Fragments arranged by size:
3543 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2701 3356 5042 6426 6694 1632
Size: 1069 655 1686 1384 268 1917
Fragments arranged by size:
1917 1686 1384 1069 655 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6782
AflIII A'CryG_T
Cuts at: 1203 3801 5878 6855 1203
Size: 2598 2077 977 1327
Fragments arranged by size:
2598 2077 1327 977
AgeI A'CCGG_T
Cuts at: 5810 5810
Size: 6979
AhdI GACnn_n'nnGTC
Cuts at: 5708 5708
Size: 6979
AleI CACnn'nnGTG
Cuts at: 1166 2775 3587 1166
Size: 1609 812 4558
Fragments arranged by size:
4558 1609 812
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3683 3765 1184
Size: 2499 82 4398
Fragments arranged by size:
4398 2499 82
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2468 2500 2468
Size: 32 6947
AlwNI CAG_nnn'CTG
Cuts at: 3670 3925 4722 6294 3670
Size: 255 797 1572 4355
Fragments arranged by size:
4355 1572 797 255
ApaI G_GGCC'C
Cuts at: 2088 3953 2088
Size: 1865 5114
ApaLI G'TGCA_C
Cuts at: 2768 3212 5036 6192 2768
Size: 444 1824 1156 3555
Fragments arranged by size:
3555 1824 1156 444
ApoI r'AATT_y
Cuts at: 1493 2872 3628 4288 4514 4666 6775 1493
Size: 1379 756 660 226 152 2109 1697
Fragments arranged by size:
2109 1697 1379 756 660 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5533 160
Size: 872 4501 1606
Fragments arranged by size:
4501 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6979
AvaI C'yCGr_G
Cuts at: 1870 1943 4304 1870
Size: 73 2361 4545
Fragments arranged by size:
4545 2361 73
BamHI G'GATC_C
Cuts at: 4309 4676 4309
Size: 367 6612
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4300 5755 618
Size: 325 217 627 2513 1455 1842
Fragments arranged by size:
2513 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2088 2259 3393 3953 4298 1391
Size: 697 171 1134 560 345 4072
Fragments arranged by size:
4072 1134 697 560 345 171
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3586 3648 928
Size: 971 1687 62 4259
Fragments arranged by size:
4259 1687 971 62
BbvCI CC'TCA_GC
Cuts at: 3015 3015
Size: 6979
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5170 5204 1903
Size: 34 3233 34 3678
Fragments arranged by size:
3678 3233 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4871 6081 4871
Size: 1210 5769
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6979
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3264 5590 136
Size: 107 122 71 631 605 1592 2326 1525
Fragments arranged by size:
2326 1592 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2622 3396 6974 2622
Size: 774 3578 2627
Fragments arranged by size:
3578 2627 774
BlpI GC'TnA_GC
Cuts at: 2194 2258 4396 2194
Size: 64 2138 4777
Fragments arranged by size:
4777 2138 64
Bme1580I G_kGCm'C
Cuts at: 1790 2088 2383 2592 2772 3216 3953 5040 6196
Size: 298 295 209 180 444 737 1087 1156
Cuts at: 6196 1790
Size: 2573
Fragments arranged by size:
2573 1156 1087 737 444 298 295 209
180
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6979
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2393 2680 2782
Size: 416 53 83 399 24 976 287 102
Cuts at: 2782 3015 3281 4710 5668 442
Size: 233 266 1429 958 1753
Fragments arranged by size:
1753 1429 976 958 416 399 287 266
233 102 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6979
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3140 3559 3857 3986 4163 5639 1239
Size: 1901 419 298 129 177 1476 2579
Fragments arranged by size:
2579 1901 1476 419 298 177 129
Bpu10I CC'TnA_GC
Cuts at: 3015 3356 3954 3015
Size: 341 598 6040
Fragments arranged by size:
6040 598 341
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2812 2987 4198 5104 5969 6267 2812
Size: 175 1211 906 865 298 3524
Fragments arranged by size:
3524 1211 906 865 298 175
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3421 4174 5642 882
Size: 617 1922 753 1468 2219
Fragments arranged by size:
2219 1922 1468 753 617
BsaAI yAC'GTr
Cuts at: 493 1204 2587 3235 4770 493
Size: 711 1383 648 1535 2702
Fragments arranged by size:
2702 1535 1383 711 648
BsaBI GATnn'nnATC
Cuts at: 6973 6973
Size: 6979
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3988 4786 5168 5803
Size: 53 83 186 936 2455 798 382 635
Cuts at: 5803 275
Size: 1451
Fragments arranged by size:
2455 1451 936 798 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2271 2409 5412 5810 6084 6231 6642 2271
Size: 138 3003 398 274 147 411 2608
Fragments arranged by size:
3003 2608 411 398 274 147 138
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2468 2498 3206 3236 3984
Size: 30 165 30 1065 30 708 30 748
Cuts at: 3984 4014 1178
Size: 30 4143
Fragments arranged by size:
4143 1065 748 708 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2759 3058 1186
Size: 1573 299 5107
Fragments arranged by size:
5107 1573 299
BseYI C'CCAG_C
Cuts at: 2039 2395 2877 3088 6182 6678 2039
Size: 356 482 211 3094 496 2340
Fragments arranged by size:
3094 2340 496 482 356 211
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3491 3602 1163
Size: 573 1755 111 4540
Fragments arranged by size:
4540 1755 573 111
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4429 5190 6218 1540
Size: 275 186 2428 761 1028 2301
Fragments arranged by size:
2428 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2613 2772 3216 5040 5125 6196 2613
Size: 159 444 1824 85 1071 3396
Fragments arranged by size:
3396 1824 1071 444 159 85
BsmI GAATG_Cn'
Cuts at: 2569 2584 4490 4583 2569
Size: 15 1906 93 4965
Fragments arranged by size:
4965 1906 93 15
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2088 2259 2383 2592 2613 2772 3216
Size: 399 298 171 124 209 21 159 444
Cuts at: 3216 3393 3953 4298 5040 5125 6196 1391
Size: 177 560 345 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 560 444 399 345 298
209 177 171 159 124 85 21
BspEI T'CCGG_A
Cuts at: 6642 6642
Size: 6979
BspHI T'CATG_A
Cuts at: 4868 6598 4868
Size: 1730 5249
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4334 844
Size: 3490 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2251 3222 4866 1394
Size: 429 428 971 1644 3507
Fragments arranged by size:
3507 1644 971 429 428
BsrDI GCAATG_nn'
Cuts at: 65 1248 2640 3597 5474 5648 65
Size: 1183 1392 957 1877 174 1396
Fragments arranged by size:
1877 1396 1392 1183 957 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4017 5623 5810 1700
Size: 110 2207 1606 187 2869
Fragments arranged by size:
2869 2207 1606 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6979
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6979
BssSI C'ACGA_G
Cuts at: 3456 5039 6051 3456
Size: 1583 1012 4384
Fragments arranged by size:
4384 1583 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6979
BstBI TT'CG_AA
Cuts at: 4114 5783 4114
Size: 1669 5310
BstEII G'GTnAC_C
Cuts at: 1184 2158 3483 1184
Size: 974 1325 4680
Fragments arranged by size:
4680 1325 974
BstXI CCAn_nnnn'nTGG
Cuts at: 2343 2343
Size: 6979
BstYI r'GATC_y
Cuts at: 1972 2622 2831 3396 4309 4676 5061 5078 6530
Size: 650 209 565 913 367 385 17 1452
Cuts at: 6530 6616 6628 6974 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 913 650 565 385 367 346
209 86 17 12
BstZ17I GTA'TAC
Cuts at: 3774 3774
Size: 6979
BtgI C'CryG_G
Cuts at: 513 1065 2004 2614 2688 513
Size: 552 939 610 74 4804
Fragments arranged by size:
4804 939 610 552 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3325 487
Size: 683 56 2099 4141
Fragments arranged by size:
4141 2099 683 56
BtsI GCAGTG_nn'
Cuts at: 2330 2613 2863 4495 5289 5309 2330
Size: 283 250 1632 794 20 4000
Fragments arranged by size:
4000 1632 794 283 250 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6979
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6944
DraI TTT'AAA
Cuts at: 4283 4283
Size: 6979
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2137 5986 809
Size: 507 821 3849 1802
Fragments arranged by size:
3849 1802 821 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2617 2916 3061
Size: 54 1425 50 275 18 787 299 145
Cuts at: 3061 3256 4426 5315 6944 8
Size: 195 1170 889 1629 43
Fragments arranged by size:
1629 1425 1170 889 787 299 275 195
145 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4426 1537
Size: 275 2614 4090
Fragments arranged by size:
4090 2614 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2720 2936 3726 4909 1353
Size: 1367 216 790 1183 3423
Fragments arranged by size:
3423 1367 1183 790 216
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2164 5566 5940 6086 387
Size: 1064 138 575 3402 374 146 1280
Fragments arranged by size:
3402 1280 1064 575 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2701 3140 3356 3559 3857 3986 4163
Size: 393 1069 439 216 203 298 129 177
Cuts at: 4163 5042 5639 6426 6694 1239
Size: 879 597 787 268 1524
Fragments arranged by size:
1524 1069 879 787 597 439 393 298
268 216 203 177 129
EcoO109I rG'GnC_Cy
Cuts at: 2084 3899 3949 3950 2084
Size: 1815 50 1 5113
Fragments arranged by size:
5113 1815 50 1
EcoRI G'AATT_C
Cuts at: 2872 4288 4666 2872
Size: 1416 378 5185
Fragments arranged by size:
5185 1416 378
FspI TGC'GCA
Cuts at: 5485 5485
Size: 6979
HaeII r_GCGC'y
Cuts at: 1678 6126 1678
Size: 4448 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2453 2485 5620 5652 5694
Size: 32 135 32 881 32 3135 32 42
Cuts at: 5694 5726 1373
Size: 32 2626
Fragments arranged by size:
3135 2626 881 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3817 4323 4569 669
Size: 988 281 1879 506 246 3079
Fragments arranged by size:
3079 1879 988 506 281 246
HindIII A'AGCT_T
Cuts at: 748 2191 748
Size: 1443 5536
HpaI GTT'AAC
Cuts at: 4569 4569
Size: 6979
KpnI G_GTAC'C
Cuts at: 4304 4304
Size: 6979
MfeI C'AATT_G
Cuts at: 4578 4578
Size: 6979
MluI A'CGCG_T
Cuts at: 6855 6855
Size: 6979
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3909 4177 6093 6277 1305
Size: 2604 268 1916 184 2007
Fragments arranged by size:
2604 2007 1916 268 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2619 2918 3063 10
Size: 54 1425 343 787 299 145 3926
Fragments arranged by size:
3926 1425 787 343 299 145 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2366 2775 2894 3209 3587 4937
Size: 648 843 357 409 119 315 378 1350
Cuts at: 4937 5296 5455 518
Size: 359 159 2042
Fragments arranged by size:
2042 1350 843 648 409 378 359 357
315 159 119
MspA1I CmG'CkG
Cuts at: 1606 2801 3088 3667 4150 4395 5072 6220 6465
Size: 1195 287 579 483 245 677 1148 245
Cuts at: 6465 1606
Size: 2120
Fragments arranged by size:
2120 1195 1148 677 579 483 287 245
245
NaeI GCC'GGC
Cuts at: 4019 4019
Size: 6979
NcoI C'CATG_G
Cuts at: 513 1065 2004 2688 513
Size: 552 939 684 4804
Fragments arranged by size:
4804 939 684 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6979
NgoMIV G'CCGG_C
Cuts at: 4017 4017
Size: 6979
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6979
NotI GC'GGCC_GC
Cuts at: 4426 4426
Size: 6979
NspI r_CATG'y
Cuts at: 1132 1229 3334 3805 4337 5882 1132
Size: 97 2105 471 532 1545 2229
Fragments arranged by size:
2229 2105 1545 532 471 97
PasI CC'CwG_GG
Cuts at: 1348 3157 3287 1348
Size: 1809 130 5040
Fragments arranged by size:
5040 1809 130
PciI A'CATG_T
Cuts at: 3801 5878 3801
Size: 2077 4902
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3088 1108
Size: 1980 4999
PfoI T'CCnGG_A
Cuts at: 2457 3117 4002 2457
Size: 660 885 5434
Fragments arranged by size:
5434 885 660
PmeI GTTT'AAAC
Cuts at: 4283 4283
Size: 6979
PmlI CAC'GTG
Cuts at: 2587 3235 2587
Size: 648 6331
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2468 2500 5048 5080 6561 6593 2468
Size: 32 2548 32 1481 32 2854
Fragments arranged by size:
2854 2548 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6979
PsiI TTA'TAA
Cuts at: 4549 4549
Size: 6979
PspOMI G'GGCC_C
Cuts at: 2084 3949 2084
Size: 1865 5114
PstI C_TGCA'G
Cuts at: 830 3675 3921 4331 830
Size: 2845 246 410 3478
Fragments arranged by size:
3478 2845 410 246
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6979
PvuII CAG'CTG
Cuts at: 2801 3088 3667 4150 2801
Size: 287 579 483 5630
Fragments arranged by size:
5630 579 483 287
SalI G'TCGA_C
Cuts at: 1936 4321 1936
Size: 2385 4594
SapI GCTCTTCn'nnn_
Cuts at: 2720 2720
Size: 6979
SbfI CC_TGCA'GG
Cuts at: 4331 4331
Size: 6979
ScaI AGT'ACT
Cuts at: 5227 6939 5227
Size: 1712 5267
SfcI C'TryA_G
Cuts at: 826 1045 3671 3822 3917 4327 5462 6143 6334
Size: 219 2626 151 95 410 1135 681 191
Cuts at: 6334 826
Size: 1471
Fragments arranged by size:
2626 1471 1135 681 410 219 191 151
95
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6979
SgrAI Cr'CCGG_yG
Cuts at: 1700 4017 1700
Size: 2317 4662
SmaI CCC'GGG
Cuts at: 1872 4306 1872
Size: 2434 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2827 3002 4177 5083 5984 6246
Size: 197 926 884 175 1175 906 901 262
Cuts at: 6246 820
Size: 1553
Fragments arranged by size:
1553 1175 926 906 901 884 262 197
175
SnaBI TAC'GTA
Cuts at: 493 4770 493
Size: 4277 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6979
SphI G_CATG'C
Cuts at: 1132 4337 1132
Size: 3205 3774
SspI AAT'ATT
Cuts at: 5 52 2696 4903 5
Size: 47 2644 2207 2081
Fragments arranged by size:
2644 2207 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6979
StyI C'CwwG_G
Cuts at: 513 1065 2004 2688 4418 513
Size: 552 939 684 1730 3074
Fragments arranged by size:
3074 1730 939 684 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3170 3496 5006 5023 5176 5361 5806
Size: 1255 1590 326 1510 17 153 185 445
Cuts at: 5806 325
Size: 1498
Fragments arranged by size:
1590 1510 1498 1255 445 326 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2053 2634 5225
Size: 274 80 33 51 1443 76 581 2591
Cuts at: 5225 6937 96
Size: 1712 138
Fragments arranged by size:
2591 1712 1443 581 274 138 80 76
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2404 2436 3206 3238 6939 6971 2404
Size: 32 770 32 3701 32 2412
Fragments arranged by size:
3701 2412 770 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6979
XbaI T'CTAG_A
Cuts at: 4315 4315
Size: 6979
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2371 2371
Size: 6979
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6979
XmaI C'CCGG_G
Cuts at: 1870 4304 1870
Size: 2434 4545
XmnI GAAnn'nnTTC
Cuts at: 5108 5108
Size: 6979
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4787 276
Size: 53 83 186 936 3253 2468
Fragments arranged by size:
3253 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PpuMI
PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI