Restriction map for
pFN21AE4572
(Circular) (Six-base) MAPSORT of: pFN21AE4572.seq Check: 1945 from: 1 to: 6928
pFN21AE4572 6928 bp
With 182 enzymes: SgfI *
March 28, 2012 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2558 3946 2558
Size: 1388 5540
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4738 278
Size: 53 83 186 936 3202 2468
Fragments arranged by size:
3202 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4271 1937
Size: 2334 4594
Acc65I G'GTAC_C
Cuts at: 4249 4249
Size: 6928
AclI AA'CG_TT
Cuts at: 1563 5055 5428 1563
Size: 3492 373 3063
Fragments arranged by size:
3492 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2270 3726 4049 4120 4991 6375 6643 1632
Size: 638 1456 323 71 871 1384 268 1917
Fragments arranged by size:
1917 1456 1384 871 638 323 268 71
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6731
AflIII A'CryG_T
Cuts at: 1203 2499 3727 5827 6804 1203
Size: 1296 1228 2100 977 1327
Fragments arranged by size:
2100 1327 1296 1228 977
AgeI A'CCGG_T
Cuts at: 5759 5759
Size: 6928
AhdI GACnn_n'nnGTC
Cuts at: 5657 5657
Size: 6928
AleI CACnn'nnGTG
Cuts at: 1166 3730 1166
Size: 2564 4364
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2153 1184
Size: 969 5959
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3497 3529 3497
Size: 32 6896
AlwNI CAG_nnn'CTG
Cuts at: 2044 2155 3298 4671 6243 2044
Size: 111 1143 1373 1572 2729
Fragments arranged by size:
2729 1572 1373 1143 111
ApaI G_GGCC'C
Cuts at: 3569 3569
Size: 6928
ApaLI G'TGCA_C
Cuts at: 4985 6141 4985
Size: 1156 5772
ApoI r'AATT_y
Cuts at: 1493 2655 2705 3158 3821 4237 4463 4615 6724
Size: 1162 50 453 663 416 226 152 2109
Cuts at: 6724 1493
Size: 1697
Fragments arranged by size:
2109 1697 1162 663 453 416 226 152
50
AseI AT'TA_AT
Cuts at: 160 1032 2400 3324 5482 160
Size: 872 1368 924 2158 1606
Fragments arranged by size:
2158 1606 1368 924 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6928
AvaI C'yCGr_G
Cuts at: 1870 1943 3938 4253 1870
Size: 73 1995 315 4545
Fragments arranged by size:
4545 1995 315 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3472 3505 3472
Size: 33 6895
BamHI G'GATC_C
Cuts at: 2543 2727 2751 4258 4625 2543
Size: 184 24 1507 367 4846
Fragments arranged by size:
4846 1507 367 184 24
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2336 4078 4148 4161 4249
Size: 325 217 627 549 1742 70 13 88
Cuts at: 4249 5704 618
Size: 1455 1842
Fragments arranged by size:
1842 1742 1455 627 549 325 217 88
70 13
BanII G_rGCy'C
Cuts at: 1391 2428 2976 3048 3569 3939 4247 1391
Size: 1037 548 72 521 370 308 4072
Fragments arranged by size:
4072 1037 548 521 370 308 72
BbeI G_GCGC'C
Cuts at: 4152 4152
Size: 6928
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5957
BbvCI CC'TCA_GC
Cuts at: 2162 3228 3908 2162
Size: 1066 680 5182
Fragments arranged by size:
5182 1066 680
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2997 3031 5119 5153 1903
Size: 34 1060 34 2088 34 3678
Fragments arranged by size:
3678 2088 1060 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3500 4820 6030 3500
Size: 1320 1210 4398
Fragments arranged by size:
4398 1320 1210
BclI T'GATC_A
Cuts at: 1548 2635 1548
Size: 1087 5841
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5539 136
Size: 107 122 71 631 605 3867 1525
Fragments arranged by size:
3867 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3393 6923 3393
Size: 3530 3398
BlpI GC'TnA_GC
Cuts at: 2970 3933 4345 2970
Size: 963 412 5553
Fragments arranged by size:
5553 963 412
Bme1580I G_kGCm'C
Cuts at: 1790 3569 4083 4989 6145 1790
Size: 1779 514 906 1156 2573
Fragments arranged by size:
2573 1779 1156 906 514
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6928
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2945 3462 4659
Size: 416 53 83 399 24 1528 517 1197
Cuts at: 4659 5617 442
Size: 958 1753
Fragments arranged by size:
1753 1528 1197 958 517 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6928
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2247 3289 3433 5588 1239
Size: 1008 1042 144 2155 2579
Fragments arranged by size:
2579 2155 1042 1008 144
Bpu10I CC'TnA_GC
Cuts at: 2162 3228 3908 2162
Size: 1066 680 5182
Fragments arranged by size:
5182 1066 680
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2411 2412 2960 5053 5918 6216 2411
Size: 1 548 2093 865 298 3123
Fragments arranged by size:
3123 2093 865 548 298 1
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5591 882
Size: 617 4092 2219
Fragments arranged by size:
4092 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3475 3828 4719 493
Size: 711 2271 353 891 2702
Fragments arranged by size:
2702 2271 891 711 353
BsaBI GATnn'nnATC
Cuts at: 6922 6922
Size: 6928
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4149 4735 5117 5752
Size: 53 83 186 936 2616 586 382 635
Cuts at: 5752 275
Size: 1451
Fragments arranged by size:
2616 1451 936 635 586 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2339 2709 5361 5759 6033 6180 6591 2339
Size: 370 2652 398 274 147 411 2676
Fragments arranged by size:
2676 2652 411 398 370 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3287 3317 1178
Size: 30 165 30 1884 30 4789
Fragments arranged by size:
4789 1884 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6928
BseYI C'CCAG_C
Cuts at: 2044 2375 3568 3690 6131 6627 2044
Size: 331 1193 122 2441 496 2345
Fragments arranged by size:
2441 2345 1193 496 331 122
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2078 4164 1163
Size: 573 342 2086 3927
Fragments arranged by size:
3927 2086 573 342
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4378 5139 6167 1540
Size: 275 186 2377 761 1028 2301
Fragments arranged by size:
2377 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2428 2976 3048 4989 5074 6145 2428
Size: 548 72 1941 85 1071 3211
Fragments arranged by size:
3211 1941 1071 548 85 72
BsmI GAATG_Cn'
Cuts at: 4439 4532 4439
Size: 93 6835
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2428 2976 3048 3569 3939 4083 4247
Size: 399 638 548 72 521 370 144 164
Cuts at: 4247 4989 5074 6145 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 638 548 521 399 370
164 144 85 72
BspEI T'CCGG_A
Cuts at: 6591 6591
Size: 6928
BspHI T'CATG_A
Cuts at: 2232 2985 4817 6547 2232
Size: 753 1832 1730 2613
Fragments arranged by size:
2613 1832 1730 753
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2558 3516 3946 4052 4283 844
Size: 1714 958 430 106 231 3489
Fragments arranged by size:
3489 1714 958 430 231 106
BsrBI CCG'CTC
Cuts at: 1394 1823 4815 1394
Size: 429 2992 3507
Fragments arranged by size:
3507 2992 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5423 5597 65
Size: 1183 4175 174 1396
Fragments arranged by size:
4175 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5572 5759 1700
Size: 110 3762 187 2869
Fragments arranged by size:
3762 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6928
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6928
BssSI C'ACGA_G
Cuts at: 4988 6000 4988
Size: 1012 5916
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3176 4221 794
Size: 2382 1045 3501
Fragments arranged by size:
3501 2382 1045
BstBI TT'CG_AA
Cuts at: 5732 5732
Size: 6928
BstEII G'GTnAC_C
Cuts at: 1184 4039 4121 1184
Size: 2855 82 3991
Fragments arranged by size:
3991 2855 82
BstXI CCAn_nnnn'nTGG
Cuts at: 3732 3732
Size: 6928
BstYI r'GATC_y
Cuts at: 1972 2151 2512 2543 2727 2751 3393 4258 4625
Size: 179 361 31 184 24 642 865 367
Cuts at: 4625 5010 5027 6479 6565 6577 6923 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 865 642 385 367 361 346
184 179 86 31 24 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2021 3484 3883 513
Size: 552 939 17 1463 399 3558
Fragments arranged by size:
3558 1463 939 552 399 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6189
Fragments arranged by size:
6189 683 56
BtsI GCAGTG_nn'
Cuts at: 3780 4444 5238 5258 3780
Size: 664 794 20 5450
Fragments arranged by size:
5450 794 664 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6928
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3878 3913 529
Size: 35 3314 35 3544
Fragments arranged by size:
3544 3314 35 35
DraI TTT'AAA
Cuts at: 3789 4232 3789
Size: 443 6485
DraIII CAC_nnn'GTG
Cuts at: 2022 4111 2022
Size: 2089 4839
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5935 809
Size: 507 4619 1802
Fragments arranged by size:
4619 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3722 3899 4062
Size: 54 1425 50 275 18 1892 177 163
Cuts at: 4062 4375 5264 6893 8
Size: 313 889 1629 43
Fragments arranged by size:
1892 1629 1425 889 313 275 177 163
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4375 1537
Size: 275 2563 4090
Fragments arranged by size:
4090 2563 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2018 4139 4858 1353
Size: 665 2121 719 3423
Fragments arranged by size:
3423 2121 719 665
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2666 5515 5889 6035 387
Size: 1064 138 1077 2849 374 146 1280
Fragments arranged by size:
2849 1280 1077 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2426 2974 3046 2426
Size: 548 72 6308
Fragments arranged by size:
6308 548 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2247 2270 3289 3433 3726 4049 4120
Size: 393 615 23 1019 144 293 323 71
Cuts at: 4120 4991 5588 6375 6643 1239
Size: 871 597 787 268 1524
Fragments arranged by size:
1524 1019 871 787 615 597 393 323
293 268 144 71 23
EcoNI CCTnn'n_nnAGG
Cuts at: 2573 2837 4010 2573
Size: 264 1173 5491
Fragments arranged by size:
5491 1173 264
EcoO109I rG'GnC_Cy
Cuts at: 3505 3565 3505
Size: 60 6868
EcoRI G'AATT_C
Cuts at: 2705 4237 4615 2705
Size: 1532 378 5018
Fragments arranged by size:
5018 1532 378
EcoRV GAT'ATC
Cuts at: 2413 3313 2413
Size: 900 6028
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2065 3415 2065
Size: 1350 5578
FspI TGC'GCA
Cuts at: 5434 5434
Size: 6928
HaeII r_GCGC'y
Cuts at: 1678 3915 4152 6075 1678
Size: 2237 237 1923 2531
Fragments arranged by size:
2531 2237 1923 237
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2712 2744 3671 3703 5569
Size: 32 135 32 1140 32 927 32 1866
Cuts at: 5569 5601 5643 5675 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1866 1140 927 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3742 4272 4518 669
Size: 988 281 1804 530 246 3079
Fragments arranged by size:
3079 1804 988 530 281 246
HindIII A'AGCT_T
Cuts at: 748 3926 748
Size: 3178 3750
HpaI GTT'AAC
Cuts at: 4518 4518
Size: 6928
KasI G'GCGC_C
Cuts at: 4148 4148
Size: 6928
KpnI G_GTAC'C
Cuts at: 4253 4253
Size: 6928
MfeI C'AATT_G
Cuts at: 4527 4527
Size: 6928
MluI A'CGCG_T
Cuts at: 6804 6804
Size: 6928
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2283 3523 6042 6226 1305
Size: 978 1240 2519 184 2007
Fragments arranged by size:
2519 2007 1240 978 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3901 4064 10
Size: 54 1425 343 2069 163 2874
Fragments arranged by size:
2874 2069 1425 343 163 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3324 3419 3425 3730 4076 4886 5245
Size: 648 2158 95 6 305 346 810 359
Cuts at: 5245 5404 518
Size: 159 2042
Fragments arranged by size:
2158 2042 810 648 359 346 305 159
95 6
MspA1I CmG'CkG
Cuts at: 1606 2369 4140 4344 5021 6169 6414 1606
Size: 763 1771 204 677 1148 245 2120
Fragments arranged by size:
2120 1771 1148 763 677 245 204
NarI GG'CG_CC
Cuts at: 4149 4149
Size: 6928
NcoI C'CATG_G
Cuts at: 513 1065 2004 3484 3883 513
Size: 552 939 1480 399 3558
Fragments arranged by size:
3558 1480 939 552 399
NdeI CA'TA_TG
Cuts at: 387 3817 387
Size: 3430 3498
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6928
NotI GC'GGCC_GC
Cuts at: 4375 4375
Size: 6928
NspI r_CATG'y
Cuts at: 1132 1229 2503 3124 3434 3731 3814 4027 4286
Size: 97 1274 621 310 297 83 213 259
Cuts at: 4286 5831 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 1274 621 310 297 259 213
97 83
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6928
PciI A'CATG_T
Cuts at: 2499 3727 5827 2499
Size: 1228 2100 3600
Fragments arranged by size:
3600 2100 1228
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2225 1108
Size: 1117 5811
PfoI T'CCnGG_A
Cuts at: 2154 2154
Size: 6928
PmeI GTTT'AAAC
Cuts at: 4232 4232
Size: 6928
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4997 5029 6510 6542 4997
Size: 32 1481 32 5383
Fragments arranged by size:
5383 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3505 3505
Size: 6928
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6928
PsiI TTA'TAA
Cuts at: 4498 4498
Size: 6928
PspOMI G'GGCC_C
Cuts at: 3565 3565
Size: 6928
PstI C_TGCA'G
Cuts at: 830 3513 4280 830
Size: 2683 767 3478
Fragments arranged by size:
3478 2683 767
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6928
PvuII CAG'CTG
Cuts at: 2369 4140 2369
Size: 1771 5157
SacI G_AGCT'C
Cuts at: 2428 2976 3048 2428
Size: 548 72 6308
Fragments arranged by size:
6308 548 72
SalI G'TCGA_C
Cuts at: 1936 4270 1936
Size: 2334 4594
SapI GCTCTTCn'nnn_
Cuts at: 4139 4139
Size: 6928
SbfI CC_TGCA'GG
Cuts at: 4280 4280
Size: 6928
ScaI AGT'ACT
Cuts at: 5176 6888 5176
Size: 1712 5216
SfcI C'TryA_G
Cuts at: 826 1045 2364 2680 3024 3509 3842 3917 4276
Size: 219 1319 316 344 485 333 75 359
Cuts at: 4276 5411 6092 6283 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1319 1135 681 485 359 344 333
316 219 191 75
SfoI GGC'GCC
Cuts at: 4150 4150
Size: 6928
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6928
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6928
SmaI CCC'GGG
Cuts at: 1872 3940 4255 1872
Size: 2068 315 4545
Fragments arranged by size:
4545 2068 315
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2390 2427 2975 5032 5933 6195
Size: 197 926 447 37 548 2057 901 262
Cuts at: 6195 820
Size: 1553
Fragments arranged by size:
2057 1553 926 901 548 447 262 197
37
SnaBI TAC'GTA
Cuts at: 493 4719 493
Size: 4226 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6928
SphI G_CATG'C
Cuts at: 1132 3124 3434 4286 1132
Size: 1992 310 852 3774
Fragments arranged by size:
3774 1992 852 310
SrfI GCCC'GGGC
Cuts at: 3940 3940
Size: 6928
SspI AAT'ATT
Cuts at: 5 52 2674 3463 4852 5
Size: 47 2622 789 1389 2081
Fragments arranged by size:
2622 2081 1389 789 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6928
StyI C'CwwG_G
Cuts at: 513 1065 2004 2603 2838 3203 3484 3883 4065
Size: 552 939 599 235 365 281 399 182
Cuts at: 4065 4367 513
Size: 302 3074
Fragments arranged by size:
3074 939 599 552 399 365 302 281
235 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3643 4955 4972 5125 5310 5755 325
Size: 1255 2063 1312 17 153 185 445 1498
Fragments arranged by size:
2063 1498 1312 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2928 4032 5174
Size: 274 80 33 51 1443 951 1104 1142
Cuts at: 5174 6886 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1142 1104 951 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3287 3319 6888 6920 3287
Size: 32 3569 32 3295
Fragments arranged by size:
3569 3295 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6928
XbaI T'CTAG_A
Cuts at: 4264 4264
Size: 6928
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4072 4072
Size: 6928
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6928
XmaI C'CCGG_G
Cuts at: 1870 3938 4253 1870
Size: 2068 315 4545
Fragments arranged by size:
4545 2068 315
XmnI GAAnn'nnTTC
Cuts at: 2898 5057 2898
Size: 2159 4769
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4736 276
Size: 53 83 186 936 3202 2468
Fragments arranged by size:
3202 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BfrBI BplI BsiWI BsmBI BstZ17I
Bsu36I FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI
SwaI