Restriction map for
pFN21AE4570
(Circular) (Six-base) MAPSORT of: pFN21AE4570.seq Check: 9132 from: 1 to: 6901
pFN21AE4570 6901 bp
With 182 enzymes: SgfI *
March 28, 2012 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2698 2943 2698
Size: 245 6656
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4711 278
Size: 53 83 186 936 3175 2468
Fragments arranged by size:
3175 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4033 4244 1937
Size: 2096 211 4594
Fragments arranged by size:
4594 2096 211
Acc65I G'GTAC_C
Cuts at: 2391 4222 2391
Size: 1831 5070
AclI AA'CG_TT
Cuts at: 1563 5028 5401 1563
Size: 3465 373 3063
Fragments arranged by size:
3465 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3319 4964 6348 6616 1632
Size: 1687 1645 1384 268 1917
Fragments arranged by size:
1917 1687 1645 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 3626 820
Size: 197 2609 4095
Fragments arranged by size:
4095 2609 197
AflIII A'CryG_T
Cuts at: 1203 2018 5800 6777 1203
Size: 815 3782 977 1327
Fragments arranged by size:
3782 1327 977 815
AgeI A'CCGG_T
Cuts at: 5732 5732
Size: 6901
AhdI GACnn_n'nnGTC
Cuts at: 3904 5630 3904
Size: 1726 5175
AleI CACnn'nnGTG
Cuts at: 1166 2692 2925 3593 1166
Size: 1526 233 668 4474
Fragments arranged by size:
4474 1526 668 233
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2039 2375 3152 1184
Size: 855 336 777 4933
Fragments arranged by size:
4933 855 777 336
AlwNI CAG_nnn'CTG
Cuts at: 2959 3190 4644 6216 2959
Size: 231 1454 1572 3644
Fragments arranged by size:
3644 1572 1454 231
ApaI G_GGCC'C
Cuts at: 2042 3063 4150 2042
Size: 1021 1087 4793
Fragments arranged by size:
4793 1087 1021
ApaLI G'TGCA_C
Cuts at: 3601 4958 6114 3601
Size: 1357 1156 4388
Fragments arranged by size:
4388 1357 1156
ApoI r'AATT_y
Cuts at: 1493 2490 4210 4436 4588 6697 1493
Size: 997 1720 226 152 2109 1697
Fragments arranged by size:
2109 1720 1697 997 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5455 160
Size: 872 4423 1606
Fragments arranged by size:
4423 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6901
AvaI C'yCGr_G
Cuts at: 1870 1943 3218 3843 4226 1870
Size: 73 1275 625 383 4545
Fragments arranged by size:
4545 1275 625 383 73
BamHI G'GATC_C
Cuts at: 4231 4598 4231
Size: 367 6534
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2028 2391 2668 2694 3143
Size: 325 217 627 241 363 277 26 449
Cuts at: 3143 3207 3767 4222 5677 618
Size: 64 560 455 1455 1842
Fragments arranged by size:
1842 1455 627 560 455 449 363 325
277 241 217 64 26
BanII G_rGCy'C
Cuts at: 1391 2042 2058 2236 2533 2793 3063 4150 4186
Size: 651 16 178 297 260 270 1087 36
Cuts at: 4186 4220 1391
Size: 34 4072
Fragments arranged by size:
4072 1087 651 297 270 260 178 36
34 16
BbeI G_GCGC'C
Cuts at: 2032 2032
Size: 6901
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2766 3556 4081 928
Size: 971 867 790 525 3748
Fragments arranged by size:
3748 971 867 790 525
BbvCI CC'TCA_GC
Cuts at: 2271 3378 3607 2271
Size: 1107 229 5565
Fragments arranged by size:
5565 1107 229
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2070 2104 2989 3023 5092 5126 1903
Size: 34 133 34 885 34 2069 34 3678
Fragments arranged by size:
3678 2069 885 133 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2363 4793 6003 2363
Size: 2430 1210 3261
Fragments arranged by size:
3261 2430 1210
BclI T'GATC_A
Cuts at: 1548 2331 1548
Size: 783 6118
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2037 3126 3185
Size: 107 122 71 631 605 365 1089 59
Cuts at: 3185 3389 5512 136
Size: 204 2123 1525
Fragments arranged by size:
2123 1525 1089 631 605 365 204 122
107 71 59
BglII A'GATC_T
Cuts at: 3159 6896 3159
Size: 3737 3164
BlpI GC'TnA_GC
Cuts at: 4318 4318
Size: 6901
Bme1580I G_kGCm'C
Cuts at: 1790 2018 2042 2283 2671 2932 3063 3146 3210
Size: 228 24 241 388 261 131 83 64
Cuts at: 3210 3534 3605 3940 4150 4962 6118 1790
Size: 324 71 335 210 812 1156 2573
Fragments arranged by size:
2573 1156 812 388 335 324 261 241
228 210 131 83 71 64 24
BmgBI CAC'GTC
Cuts at: 1318 2109 1318
Size: 791 6110
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2674 3934 4632
Size: 416 53 83 399 24 1257 1260 698
Cuts at: 4632 5590 442
Size: 958 1753
Fragments arranged by size:
1753 1260 1257 958 698 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6901
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3106 3467 3622 3106
Size: 361 155 6385
Fragments arranged by size:
6385 361 155
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3076 3560 3803 5561 1239
Size: 1837 484 243 1758 2579
Fragments arranged by size:
2579 1837 1758 484 243
Bpu10I CC'TnA_GC
Cuts at: 2271 3378 3607 2271
Size: 1107 229 5565
Fragments arranged by size:
5565 1107 229
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3215 4058 5026 5891 6189 3215
Size: 843 968 865 298 3927
Fragments arranged by size:
3927 968 865 843 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3509 3671 3844 5564 882
Size: 617 2010 162 173 1720 2219
Fragments arranged by size:
2219 2010 1720 617 173 162
BsaAI yAC'GTr
Cuts at: 493 1204 2019 2257 2344 4692 493
Size: 711 815 238 87 2348 2702
Fragments arranged by size:
2702 2348 815 711 238 87
BsaBI GATnn'nnATC
Cuts at: 6895 6895
Size: 6901
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2029 4708 5090 5725
Size: 53 83 186 936 496 2679 382 635
Cuts at: 5725 275
Size: 1451
Fragments arranged by size:
2679 1451 936 635 496 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4026 5334 5732 6006 6153 6564 4026
Size: 1308 398 274 147 411 4363
Fragments arranged by size:
4363 1308 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2003 2033 2298 2328 1178
Size: 30 165 30 600 30 265 30 5751
Fragments arranged by size:
5751 600 265 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2891 3929 4035 1186
Size: 1705 1038 106 4052
Fragments arranged by size:
4052 1705 1038 106
BseYI C'CCAG_C
Cuts at: 2424 2616 3888 3963 4082 4164 6104 6600 2424
Size: 192 1272 75 119 82 1940 496 2725
Fragments arranged by size:
2725 1940 1272 496 192 119 82 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2417 2426 2972 3355 3852 3950 1163
Size: 573 681 9 546 383 497 98 4114
Fragments arranged by size:
4114 681 573 546 497 383 98 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2074 2140 2986 4156 4351 5112
Size: 275 186 73 66 846 1170 195 761
Cuts at: 5112 6140 1540
Size: 1028 2301
Fragments arranged by size:
2301 1170 1028 846 761 275 195 186
73 66
BsiHKAI G_wGCw'C
Cuts at: 2793 2919 3435 3605 3657 4962 5047 6118 2793
Size: 126 516 170 52 1305 85 1071 3576
Fragments arranged by size:
3576 1305 1071 516 170 126 85 52
BsmI GAATG_Cn'
Cuts at: 3173 4412 4505 3173
Size: 1239 93 5569
Fragments arranged by size:
5569 1239 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2042 2058 2236 2283 2533 2671
Size: 399 228 24 16 178 47 250 138
Cuts at: 2671 2793 2919 2932 3063 3146 3210 3435 3534
Size: 122 126 13 131 83 64 225 99
Cuts at: 3534 3605 3657 3940 4150 4186 4220 4962 5047
Size: 71 52 283 210 36 34 742 85
Cuts at: 5047 6118 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 283 250 228 225
210 178 138 131 126 122 99 85
83 71 64 52 47 36 34 24
16 13
BspEI T'CCGG_A
Cuts at: 6564 6564
Size: 6901
BspHI T'CATG_A
Cuts at: 3247 3576 4790 6520 3247
Size: 329 1214 1730 3628
Fragments arranged by size:
3628 1730 1214 329
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2127 2403 2698 2724 2943 4256 844
Size: 1283 276 295 26 219 1313 3489
Fragments arranged by size:
3489 1313 1283 295 276 219 26
BsrBI CCG'CTC
Cuts at: 1394 1823 3919 4788 1394
Size: 429 2096 869 3507
Fragments arranged by size:
3507 2096 869 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4077 5396 5570 65
Size: 1183 2829 1319 174 1396
Fragments arranged by size:
2829 1396 1319 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2100 2135 2150 2671 2703 5545 5732
Size: 110 290 35 15 521 32 2842 187
Cuts at: 5732 1700
Size: 2869
Fragments arranged by size:
2869 2842 521 290 187 110 35 32
15
BsrGI T'GTAC_A
Cuts at: 96 2859 96
Size: 2763 4138
BssHII G'CGCG_C
Cuts at: 1925 2131 1925
Size: 206 6695
BssSI C'ACGA_G
Cuts at: 4117 4961 5973 4117
Size: 844 1012 5045
Fragments arranged by size:
5045 1012 844
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6901
BstBI TT'CG_AA
Cuts at: 5705 5705
Size: 6901
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6901
BstXI CCAn_nnnn'nTGG
Cuts at: 2649 2649
Size: 6901
BstYI r'GATC_y
Cuts at: 1972 3159 3669 3948 4231 4598 4983 5000 6452
Size: 1187 510 279 283 367 385 17 1452
Cuts at: 6452 6538 6550 6896 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1187 510 385 367 346 283
279 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3257 3257
Size: 6901
BtgI C'CryG_G
Cuts at: 513 1065 2754 513
Size: 552 1689 4660
Fragments arranged by size:
4660 1689 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3471 487
Size: 683 56 2245 3917
Fragments arranged by size:
3917 2245 683 56
BtsI GCAGTG_nn'
Cuts at: 2698 3069 4417 5211 5231 2698
Size: 371 1348 794 20 4368
Fragments arranged by size:
4368 1348 794 371 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6901
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2440 2475 2536 2571 529
Size: 35 1876 35 61 35 4859
Fragments arranged by size:
4859 1876 61 35 35 35
DraI TTT'AAA
Cuts at: 4205 4205
Size: 6901
DraIII CAC_nnn'GTG
Cuts at: 2974 2974
Size: 6901
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2768 5908 809
Size: 507 1452 3140 1802
Fragments arranged by size:
3140 1802 1452 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2071 2137 2565
Size: 54 1425 50 275 18 241 66 428
Cuts at: 2565 2976 3984 4064 4153 4192 4348 5237 6866
Size: 411 1008 80 89 39 156 889 1629
Cuts at: 6866 8
Size: 43
Fragments arranged by size:
1629 1425 1008 889 428 411 275 241
156 89 80 66 54 50 43 39
18
EagI C'GGCC_G
Cuts at: 1537 1812 2071 2137 4153 4348 1537
Size: 275 259 66 2016 195 4090
Fragments arranged by size:
4090 2016 275 259 195 66
EarI CTCTTCn'nnn_
Cuts at: 1353 2373 4831 1353
Size: 1020 2458 3423
Fragments arranged by size:
3423 2458 1020
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2771 5488 5862 6008 387
Size: 1064 138 1182 2717 374 146 1280
Fragments arranged by size:
2717 1280 1182 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2791 2791
Size: 6901
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3076 3319 3560 3803 4964 5561 6348
Size: 393 1444 243 241 243 1161 597 787
Cuts at: 6348 6616 1239
Size: 268 1524
Fragments arranged by size:
1524 1444 1161 787 597 393 268 243
243 241
EcoNI CCTnn'n_nnAGG
Cuts at: 2783 3239 3686 2783
Size: 456 447 5998
Fragments arranged by size:
5998 456 447
EcoO109I rG'GnC_Cy
Cuts at: 2038 2039 2428 3271 4147 2038
Size: 1 389 843 876 4792
Fragments arranged by size:
4792 876 843 389 1
EcoRI G'AATT_C
Cuts at: 2490 4210 4588 2490
Size: 1720 378 4803
Fragments arranged by size:
4803 1720 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2209 3343 2209
Size: 1134 5767
FspI TGC'GCA
Cuts at: 3317 5407 3317
Size: 2090 4811
HaeII r_GCGC'y
Cuts at: 1678 2032 3626 6048 1678
Size: 354 1594 2422 2531
Fragments arranged by size:
2531 2422 1594 354
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2432 2464 3212 3244 3890
Size: 32 135 32 860 32 748 32 646
Cuts at: 3890 3922 5542 5574 5616 5648 1373
Size: 32 1620 32 42 32 2626
Fragments arranged by size:
2626 1620 860 748 646 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4245 4491 669
Size: 988 281 2307 246 3079
Fragments arranged by size:
3079 2307 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6901
HpaI GTT'AAC
Cuts at: 4491 4491
Size: 6901
KasI G'GCGC_C
Cuts at: 2028 2028
Size: 6901
KpnI G_GTAC'C
Cuts at: 2395 4226 2395
Size: 1831 5070
MfeI C'AATT_G
Cuts at: 4500 4500
Size: 6901
MluI A'CGCG_T
Cuts at: 6777 6777
Size: 6901
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6015 6199 1305
Size: 4710 184 2007
Fragments arranged by size:
4710 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2567 4194 10
Size: 54 1425 343 735 1627 2717
Fragments arranged by size:
2717 1627 1425 735 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2692 2925 3032 3038 3134 3593 4859
Size: 648 1526 233 107 6 96 459 1266
Cuts at: 4859 5218 5377 518
Size: 359 159 2042
Fragments arranged by size:
2042 1526 1266 648 459 359 233 159
107 96 6
MspA1I CmG'CkG
Cuts at: 1606 2076 2616 2756 2767 2874 2944 3665 4168
Size: 470 540 140 11 107 70 721 503
Cuts at: 4168 4317 4994 6142 6387 1606
Size: 149 677 1148 245 2120
Fragments arranged by size:
2120 1148 721 677 540 503 470 245
149 140 107 70 11
NaeI GCC'GGC
Cuts at: 2102 2137 2705 2102
Size: 35 568 6298
Fragments arranged by size:
6298 568 35
NarI GG'CG_CC
Cuts at: 2029 2029
Size: 6901
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6349
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6901
NgoMIV G'CCGG_C
Cuts at: 2100 2135 2703 2100
Size: 35 568 6298
Fragments arranged by size:
6298 568 35
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6901
NotI GC'GGCC_GC
Cuts at: 4348 4348
Size: 6901
NspI r_CATG'y
Cuts at: 1132 1229 3037 3089 3963 4259 5804 1132
Size: 97 1808 52 874 296 1545 2229
Fragments arranged by size:
2229 1808 1545 874 296 97 52
PasI CC'CwG_GG
Cuts at: 1348 2170 1348
Size: 822 6079
PciI A'CATG_T
Cuts at: 5800 5800
Size: 6901
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2691 1108
Size: 1583 5318
PfoI T'CCnGG_A
Cuts at: 3078 3099 3648 3672 3078
Size: 21 549 24 6307
Fragments arranged by size:
6307 549 24 21
PmeI GTTT'AAAC
Cuts at: 4205 4205
Size: 6901
PmlI CAC'GTG
Cuts at: 2019 2344 2019
Size: 325 6576
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4970 5002 6483 6515 4970
Size: 32 1481 32 5356
Fragments arranged by size:
5356 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3271 3271
Size: 6901
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6901
PsiI TTA'TAA
Cuts at: 4471 4471
Size: 6901
PspOMI G'GGCC_C
Cuts at: 2038 3059 4146 2038
Size: 1021 1087 4793
Fragments arranged by size:
4793 1087 1021
PstI C_TGCA'G
Cuts at: 830 2124 2268 3240 3408 4253 830
Size: 1294 144 972 168 845 3478
Fragments arranged by size:
3478 1294 972 845 168 144
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6901
PvuII CAG'CTG
Cuts at: 2616 2874 4168 2616
Size: 258 1294 5349
Fragments arranged by size:
5349 1294 258
SacI G_AGCT'C
Cuts at: 2793 2793
Size: 6901
SacII CC_GC'GG
Cuts at: 2757 2757
Size: 6901
SalI G'TCGA_C
Cuts at: 1936 4243 1936
Size: 2307 4594
SbfI CC_TGCA'GG
Cuts at: 2124 3408 4253 2124
Size: 1284 845 4772
Fragments arranged by size:
4772 1284 845
ScaI AGT'ACT
Cuts at: 3302 5149 6861 3302
Size: 1847 1712 3342
Fragments arranged by size:
3342 1847 1712
SexAI A'CCwGG_T
Cuts at: 2520 3770 2520
Size: 1250 5651
SfcI C'TryA_G
Cuts at: 826 1045 2120 2264 3236 3404 4123 4249 5384
Size: 219 1075 144 972 168 719 126 1135
Cuts at: 5384 6065 6256 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1075 972 719 681 219 191
168 144 126
SfoI GGC'GCC
Cuts at: 2030 2030
Size: 6901
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6901
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6901
SmaI CCC'GGG
Cuts at: 1872 3845 4228 1872
Size: 1973 383 4545
Fragments arranged by size:
4545 1973 383
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3194 3626 4037 5005 5906 6168
Size: 197 926 1251 432 411 968 901 262
Cuts at: 6168 820
Size: 1553
Fragments arranged by size:
1553 1251 968 926 901 432 411 262
197
SnaBI TAC'GTA
Cuts at: 493 4692 493
Size: 4199 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6901
SphI G_CATG'C
Cuts at: 1132 4259 1132
Size: 3127 3774
SspI AAT'ATT
Cuts at: 5 52 4825 5
Size: 47 4773 2081
Fragments arranged by size:
4773 2081 47
StuI AGG'CCT
Cuts at: 1517 2325 2625 3383 3788 1517
Size: 808 300 758 405 4630
Fragments arranged by size:
4630 808 758 405 300
StyI C'CwwG_G
Cuts at: 513 1065 4340 513
Size: 552 3275 3074
Fragments arranged by size:
3275 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2066 2486 3000 4928 4945 5098 5283
Size: 1255 486 420 514 1928 17 153 185
Cuts at: 5283 5728 325
Size: 445 1498
Fragments arranged by size:
1928 1498 1255 514 486 445 420 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2859 3300 5147
Size: 274 80 33 51 1443 882 441 1847
Cuts at: 5147 6859 96
Size: 1712 138
Fragments arranged by size:
1847 1712 1443 882 441 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2003 2035 2298 2330 3814 3846 6861 6893 2003
Size: 32 263 32 1484 32 3015 32 2011
Fragments arranged by size:
3015 2011 1484 263 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6901
XbaI T'CTAG_A
Cuts at: 4237 4237
Size: 6901
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6901
XmaI C'CCGG_G
Cuts at: 1870 3843 4226 1870
Size: 1973 383 4545
Fragments arranged by size:
4545 1973 383
XmnI GAAnn'nnTTC
Cuts at: 4133 5030 4133
Size: 897 6004
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4709 276
Size: 53 83 186 936 3175 2468
Fragments arranged by size:
3175 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I EcoRV FseI FspAI NruI NsiI PacI PspXI
PsrI RsrII SanDI SapI SfiI SrfI SwaI XcmI