Restriction map for
pFN21AE4569
(Circular) (Six-base) MAPSORT of: pFN21AE4569.seq Check: 803 from: 1 to: 6877
pFN21AE4569 6877 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2289 4687 278
Size: 53 83 186 936 753 2398 2468
Fragments arranged by size:
2468 2398 936 753 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3447 4220 1937
Size: 1510 773 4594
Fragments arranged by size:
4594 1510 773
Acc65I G'GTAC_C
Cuts at: 3073 4198 3073
Size: 1125 5752
AclI AA'CG_TT
Cuts at: 1563 5004 5377 1563
Size: 3441 373 3063
Fragments arranged by size:
3441 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3481 3498 4940 6324 6592 1632
Size: 1849 17 1442 1384 268 1917
Fragments arranged by size:
1917 1849 1442 1384 268 17
AfeI AGC'GCT
Cuts at: 2585 2585
Size: 6877
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6680
AflIII A'CryG_T
Cuts at: 1203 2115 3411 3459 5776 6753 1203
Size: 912 1296 48 2317 977 1327
Fragments arranged by size:
2317 1327 1296 977 912 48
AgeI A'CCGG_T
Cuts at: 5708 5708
Size: 6877
AhdI GACnn_n'nnGTC
Cuts at: 2557 3886 3943 5606 2557
Size: 1329 57 1663 3828
Fragments arranged by size:
3828 1663 1329 57
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6877
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6877
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2069 2101 2069
Size: 32 6845
AlwNI CAG_nnn'CTG
Cuts at: 4620 6192 4620
Size: 1572 5305
ApaI G_GGCC'C
Cuts at: 4013 4013
Size: 6877
ApaLI G'TGCA_C
Cuts at: 4934 6090 4934
Size: 1156 5721
ApoI r'AATT_y
Cuts at: 1493 4186 4412 4564 6673 1493
Size: 2693 226 152 2109 1697
Fragments arranged by size:
2693 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5431 160
Size: 872 4399 1606
Fragments arranged by size:
4399 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6877
AvaI C'yCGr_G
Cuts at: 1870 1943 2319 2344 2505 3237 3842 4202 1870
Size: 73 376 25 161 732 605 360 4545
Fragments arranged by size:
4545 732 605 376 360 161 73 25
AvrII C'CTAG_G
Cuts at: 3068 3068
Size: 6877
BamHI G'GATC_C
Cuts at: 2694 3042 4207 4574 2694
Size: 348 1165 367 4997
Fragments arranged by size:
4997 1165 367 348
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3073 3256 3364 4198 5653
Size: 325 217 627 1286 183 108 834 1455
Cuts at: 5653 618
Size: 1842
Fragments arranged by size:
1842 1455 1286 834 627 325 217 183
108
BanII G_rGCy'C
Cuts at: 1391 2513 4013 4196 1391
Size: 1122 1500 183 4072
Fragments arranged by size:
4072 1500 1122 183
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3822 4069 928
Size: 971 1923 247 3736
Fragments arranged by size:
3736 1923 971 247
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2831 2865 3413 3447 5068
Size: 34 50 34 810 34 548 34 1621
Cuts at: 5068 5102 1903
Size: 34 3678
Fragments arranged by size:
3678 1621 810 548 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4769 5979 4769
Size: 1210 5667
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6877
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2253 2538 2598
Size: 107 122 71 631 605 581 285 60
Cuts at: 2598 5488 136
Size: 2890 1525
Fragments arranged by size:
2890 1525 631 605 581 285 122 107
71 60
BglII A'GATC_T
Cuts at: 6872 6872
Size: 6877
BlpI GC'TnA_GC
Cuts at: 4294 4294
Size: 6877
Bme1580I G_kGCm'C
Cuts at: 1790 2793 3271 3659 4013 4938 6094 1790
Size: 1003 478 388 354 925 1156 2573
Fragments arranged by size:
2573 1156 1003 925 478 388 354
BmgBI CAC'GTC
Cuts at: 1318 2047 3412 3460 1318
Size: 729 1365 48 4735
Fragments arranged by size:
4735 1365 729 48
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3565 4608 5566
Size: 416 53 83 399 24 2148 1043 958
Cuts at: 5566 442
Size: 1753
Fragments arranged by size:
2148 1753 1043 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6877
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3709 5537 1239
Size: 2470 1828 2579
Fragments arranged by size:
2579 2470 1828
Bpu10I CC'TnA_GC
Cuts at: 4014 4014
Size: 6877
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3800 5002 5867 6165 3800
Size: 1202 865 298 4512
Fragments arranged by size:
4512 1202 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2453 4148 5540 882
Size: 617 954 1695 1392 2219
Fragments arranged by size:
2219 1695 1392 954 617
BsaAI yAC'GTr
Cuts at: 493 1204 3736 4668 493
Size: 711 2532 932 2702
Fragments arranged by size:
2702 2532 932 711
BsaBI GATnn'nnATC
Cuts at: 6871 6871
Size: 6877
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2286 2558 2835 4684
Size: 53 83 186 936 753 272 277 1849
Cuts at: 4684 5066 5701 275
Size: 382 635 1451
Fragments arranged by size:
1849 1451 936 753 635 382 277 272
186 83 53
BsaWI w'CCGG_w
Cuts at: 2845 3039 3466 5310 5708 5982 6129 6540 2845
Size: 194 427 1844 398 274 147 411 3182
Fragments arranged by size:
3182 1844 427 411 398 274 194 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2069 2099 2747 2777 3749
Size: 30 165 30 666 30 648 30 972
Cuts at: 3749 3779 1178
Size: 30 4276
Fragments arranged by size:
4276 972 666 648 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6877
BseYI C'CCAG_C
Cuts at: 2153 2606 2679 6080 6576 2153
Size: 453 73 3401 496 2454
Fragments arranged by size:
3401 2454 496 453 73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2263 3608 1163
Size: 573 527 1345 4432
Fragments arranged by size:
4432 1345 573 527
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2660 3004 3040 4327 5088 6116
Size: 275 186 659 344 36 1287 761 1028
Cuts at: 6116 1540
Size: 2301
Fragments arranged by size:
2301 1287 1028 761 659 344 275 186
36
BsiHKAI G_wGCw'C
Cuts at: 3567 4938 5023 6094 3567
Size: 1371 85 1071 4350
Fragments arranged by size:
4350 1371 1071 85
BsmI GAATG_Cn'
Cuts at: 4388 4481 4388
Size: 93 6784
BsmBI CGTCTCn'nnnn_
Cuts at: 2826 3213 2826
Size: 387 6490
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2513 2793 3271 3567 3659 4013 4196
Size: 399 723 280 478 296 92 354 183
Cuts at: 4196 4938 5023 6094 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 723 478 399 354 296
280 183 92 85
BspEI T'CCGG_A
Cuts at: 2845 3466 6540 2845
Size: 621 3074 3182
Fragments arranged by size:
3182 3074 621
BspHI T'CATG_A
Cuts at: 4766 6496 4766
Size: 1730 5147
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2059 2422 4122 4232 844
Size: 1215 363 1700 110 3489
Fragments arranged by size:
3489 1700 1215 363 110
BsrBI CCG'CTC
Cuts at: 1394 1823 2226 4764 1394
Size: 429 403 2538 3507
Fragments arranged by size:
3507 2538 429 403
BsrDI GCAATG_nn'
Cuts at: 65 1248 4135 5372 5546 65
Size: 1183 2887 1237 174 1396
Fragments arranged by size:
2887 1396 1237 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2144 2684 2958 3969 5521 5708 1700
Size: 110 334 540 274 1011 1552 187 2869
Fragments arranged by size:
2869 1552 1011 540 334 274 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6877
BssHII G'CGCG_C
Cuts at: 1925 2178 2229 2231 2575 1925
Size: 253 51 2 344 6227
Fragments arranged by size:
6227 344 253 51 2
BssSI C'ACGA_G
Cuts at: 2618 4937 5949 2618
Size: 2319 1012 3546
Fragments arranged by size:
3546 2319 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2047 794
Size: 1253 5624
BstBI TT'CG_AA
Cuts at: 5681 5681
Size: 6877
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6877
BstXI CCAn_nnnn'nTGG
Cuts at: 3559 3559
Size: 6877
BstYI r'GATC_y
Cuts at: 1972 2694 3042 4207 4574 4959 4976 6428 6514
Size: 722 348 1165 367 385 17 1452 86
Cuts at: 6514 6526 6872 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1165 722 385 367 348 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2250 3252 513
Size: 552 1185 1002 4138
Fragments arranged by size:
4138 1185 1002 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2661 2835 2955 3024 3120 487
Size: 683 56 1435 174 120 69 96 4244
Fragments arranged by size:
4244 1435 683 174 120 96 69 56
BtsI GCAGTG_nn'
Cuts at: 2173 2275 3627 4393 5187 5207 2173
Size: 102 1352 766 794 20 3843
Fragments arranged by size:
3843 1352 794 766 102 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6877
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6842
DraI TTT'AAA
Cuts at: 4181 4181
Size: 6877
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5884 809
Size: 507 4568 1802
Fragments arranged by size:
4568 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3001 3292 3376
Size: 54 1425 50 275 18 1171 291 84
Cuts at: 3376 3795 4023 4063 4324 5213 6842 8
Size: 419 228 40 261 889 1629 43
Fragments arranged by size:
1629 1425 1171 889 419 291 275 261
228 84 54 50 43 40 18
EagI C'GGCC_G
Cuts at: 1537 1812 3001 4324 1537
Size: 275 1189 1323 4090
Fragments arranged by size:
4090 1323 1189 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3137 4807 1353
Size: 1784 1670 3423
Fragments arranged by size:
3423 1784 1670
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3176 5464 5838 5984 387
Size: 1064 138 1587 2288 374 146 1280
Fragments arranged by size:
2288 1587 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3481 3498 3709 4940 5537 6324 6592
Size: 393 1849 17 211 1231 597 787 268
Cuts at: 6592 1239
Size: 1524
Fragments arranged by size:
1849 1524 1231 787 597 393 268 211
17
EcoO109I rG'GnC_Cy
Cuts at: 2866 3501 3839 3913 4009 4010 2866
Size: 635 338 74 96 1 5733
Fragments arranged by size:
5733 635 338 96 74 1
EcoRI G'AATT_C
Cuts at: 4186 4564 4186
Size: 378 6499
FspI TGC'GCA
Cuts at: 5383 5383
Size: 6877
HaeII r_GCGC'y
Cuts at: 1678 2039 2587 2761 6024 1678
Size: 361 548 174 3263 2531
Fragments arranged by size:
3263 2531 548 361 174
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2543 2575 3872 3904 3929
Size: 32 135 32 971 32 1297 32 25
Cuts at: 3929 3961 5518 5550 5592 5624 1373
Size: 32 1557 32 42 32 2626
Fragments arranged by size:
2626 1557 1297 971 135 42 32 32
32 32 32 32 32 25
HincII GTy'rAC
Cuts at: 669 1657 1938 2290 4221 4467 669
Size: 988 281 352 1931 246 3079
Fragments arranged by size:
3079 1931 988 352 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6877
HpaI GTT'AAC
Cuts at: 4467 4467
Size: 6877
KpnI G_GTAC'C
Cuts at: 3077 4202 3077
Size: 1125 5752
MfeI C'AATT_G
Cuts at: 4476 4476
Size: 6877
MluI A'CGCG_T
Cuts at: 2115 6753 2115
Size: 4638 2239
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3835 3911 5991 6175 1305
Size: 2530 76 2080 184 2007
Fragments arranged by size:
2530 2080 2007 184 76
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3294 10
Size: 54 1425 343 1462 3593
Fragments arranged by size:
3593 1462 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2102 2622 3992 4052 4835 5194 5353
Size: 648 936 520 1370 60 783 359 159
Cuts at: 5353 518
Size: 2042
Fragments arranged by size:
2042 1370 936 783 648 520 359 159
60
MspA1I CmG'CkG
Cuts at: 1606 2252 2328 2355 2382 2572 2893 3853 4125
Size: 646 76 27 27 190 321 960 272
Cuts at: 4125 4165 4293 4970 6118 6363 1606
Size: 40 128 677 1148 245 2120
Fragments arranged by size:
2120 1148 960 677 646 321 272 245
190 128 76 40 27 27
NaeI GCC'GGC
Cuts at: 2686 2686
Size: 6877
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6325
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6877
NgoMIV G'CCGG_C
Cuts at: 2684 2684
Size: 6877
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6877
NotI GC'GGCC_GC
Cuts at: 4324 4324
Size: 6877
NruI TCG'CGA
Cuts at: 2709 2709
Size: 6877
NspI r_CATG'y
Cuts at: 1132 1229 2627 4235 5780 1132
Size: 97 1398 1608 1545 2229
Fragments arranged by size:
2229 1608 1545 1398 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6877
PciI A'CATG_T
Cuts at: 5776 5776
Size: 6877
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2401 1108
Size: 1293 5584
PfoI T'CCnGG_A
Cuts at: 2730 2730
Size: 6877
PmeI GTTT'AAAC
Cuts at: 4181 4181
Size: 6877
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2069 2101 3644 3676 4946 4978 6459 6491 2069
Size: 32 1543 32 1270 32 1481 32 2455
Fragments arranged by size:
2455 1543 1481 1270 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2866 3913 2866
Size: 1047 5830
PshAI GACnn'nnGTC
Cuts at: 1613 3132 3246 3462 1613
Size: 1519 114 216 5028
Fragments arranged by size:
5028 1519 216 114
PsiI TTA'TAA
Cuts at: 4447 4447
Size: 6877
PspOMI G'GGCC_C
Cuts at: 4009 4009
Size: 6877
PstI C_TGCA'G
Cuts at: 830 2022 2043 3966 4229 830
Size: 1192 21 1923 263 3478
Fragments arranged by size:
3478 1923 1192 263 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6877
PvuII CAG'CTG
Cuts at: 3853 4165 3853
Size: 312 6565
SacII CC_GC'GG
Cuts at: 2253 2253
Size: 6877
SalI G'TCGA_C
Cuts at: 1936 4219 1936
Size: 2283 4594
SanDI GG'GwC_CC
Cuts at: 2866 2866
Size: 6877
SapI GCTCTTCn'nnn_
Cuts at: 3137 3137
Size: 6877
SbfI CC_TGCA'GG
Cuts at: 4229 4229
Size: 6877
ScaI AGT'ACT
Cuts at: 5125 6837 5125
Size: 1712 5165
SfcI C'TryA_G
Cuts at: 826 1045 2018 2039 3962 4225 5360 6041 6232
Size: 219 973 21 1923 263 1135 681 191
Cuts at: 6232 826
Size: 1471
Fragments arranged by size:
1923 1471 1135 973 681 263 219 191
21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6877
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6877
SmaI CCC'GGG
Cuts at: 1872 2346 2507 3844 4204 1872
Size: 474 161 1337 360 4545
Fragments arranged by size:
4545 1337 474 360 161
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3815 4981 5882 6144 820
Size: 197 926 1872 1166 901 262 1553
Fragments arranged by size:
1872 1553 1166 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4668 493
Size: 4175 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6877
SphI G_CATG'C
Cuts at: 1132 2627 4235 1132
Size: 1495 1608 3774
Fragments arranged by size:
3774 1608 1495
SspI AAT'ATT
Cuts at: 5 52 4801 5
Size: 47 4749 2081
Fragments arranged by size:
4749 2081 47
StuI AGG'CCT
Cuts at: 1517 3612 1517
Size: 2095 4782
StyI C'CwwG_G
Cuts at: 513 1065 2418 3068 4316 513
Size: 552 1353 650 1248 3074
Fragments arranged by size:
3074 1353 1248 650 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2405 3547 4904 4921 5074 5259 5704
Size: 1255 825 1142 1357 17 153 185 445
Cuts at: 5704 325
Size: 1498
Fragments arranged by size:
1498 1357 1255 1142 825 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5123 6835 96
Size: 274 80 33 51 1443 3146 1712 138
Fragments arranged by size:
3146 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2686 2718 2747 2779 6837 6869 2686
Size: 32 29 32 4058 32 2694
Fragments arranged by size:
4058 2694 32 32 32 29
Tth111I GACn'n_nGTC
Cuts at: 1316 2633 2864 3332 1316
Size: 1317 231 468 4861
Fragments arranged by size:
4861 1317 468 231
XbaI T'CTAG_A
Cuts at: 4213 4213
Size: 6877
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3733 3733
Size: 6877
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6877
XmaI C'CCGG_G
Cuts at: 1870 2344 2505 3842 4202 1870
Size: 474 161 1337 360 4545
Fragments arranged by size:
4545 1337 474 360 161
XmnI GAAnn'nnTTC
Cuts at: 3782 5006 3782
Size: 1224 5653
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2287 4685 276
Size: 53 83 186 936 753 2398 2468
Fragments arranged by size:
2468 2398 936 753 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NruI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacII SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI
BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI
FspAI KasI NarI NsiI PacI PmlI PspXI PsrI
RsrII SacI SexAI SfiI SfoI SrfI SwaI