Restriction map for
pFN21AE4567
(Circular) (Six-base) MAPSORT of: pFN21AE4567.seq Check: 6237 from: 1 to: 6862
pFN21AE4567 6862 bp
With 182 enzymes: SgfI *
July 6, 2012 17:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4672 278
Size: 53 83 186 936 3136 2468
Fragments arranged by size:
3136 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3614 4205 1937
Size: 1677 591 4594
Fragments arranged by size:
4594 1677 591
Acc65I G'GTAC_C
Cuts at: 4183 4183
Size: 6862
AclI AA'CG_TT
Cuts at: 1563 4989 5362 1563
Size: 3426 373 3063
Fragments arranged by size:
3426 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2571 3582 4925 6309 6577 1632
Size: 939 1011 1343 1384 268 1917
Fragments arranged by size:
1917 1384 1343 1011 939 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6665
AflIII A'CryG_T
Cuts at: 1203 5761 6738 1203
Size: 4558 977 1327
Fragments arranged by size:
4558 1327 977
AgeI A'CCGG_T
Cuts at: 5693 5693
Size: 6862
AhdI GACnn_n'nnGTC
Cuts at: 5591 5591
Size: 6862
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6862
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4152 1184
Size: 2968 3894
AlwNI CAG_nnn'CTG
Cuts at: 2861 4605 6177 2861
Size: 1744 1572 3546
Fragments arranged by size:
3546 1744 1572
ApaLI G'TGCA_C
Cuts at: 4919 6075 4919
Size: 1156 5706
ApoI r'AATT_y
Cuts at: 1493 2289 2309 3066 3278 3316 3638 3916 4032
Size: 796 20 757 212 38 322 278 116
Cuts at: 4032 4171 4397 4549 6658 1493
Size: 139 226 152 2109 1697
Fragments arranged by size:
2109 1697 796 757 322 278 226 212
152 139 116 38 20
AseI AT'TA_AT
Cuts at: 160 1032 2397 3354 5416 160
Size: 872 1365 957 2062 1606
Fragments arranged by size:
2062 1606 1365 957 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6862
AvaI C'yCGr_G
Cuts at: 1870 1943 4187 1870
Size: 73 2244 4545
Fragments arranged by size:
4545 2244 73
BamHI G'GATC_C
Cuts at: 4192 4559 4192
Size: 367 6495
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4183 5638 618
Size: 325 217 627 2396 1455 1842
Fragments arranged by size:
2396 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4181 1391
Size: 2790 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2559 2861 3278 3878 928
Size: 971 660 302 417 600 3912
Fragments arranged by size:
3912 971 660 600 417 302
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5053 5087 1903
Size: 34 3116 34 3678
Fragments arranged by size:
3678 3116 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2891 4754 5964 2891
Size: 1863 1210 3789
Fragments arranged by size:
3789 1863 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6862
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2660 5473 136
Size: 107 122 71 631 605 988 2813 1525
Fragments arranged by size:
2813 1525 988 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6857 6857
Size: 6862
BlpI GC'TnA_GC
Cuts at: 2938 4279 2938
Size: 1341 5521
Bme1580I G_kGCm'C
Cuts at: 1790 2982 4923 6079 1790
Size: 1192 1941 1156 2573
Fragments arranged by size:
2573 1941 1192 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6862
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2293 4593 5551
Size: 416 53 83 399 24 876 2300 958
Cuts at: 5551 442
Size: 1753
Fragments arranged by size:
2300 1753 958 876 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2336 3767 1056
Size: 1280 1431 4151
Fragments arranged by size:
4151 1431 1280
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2699 2822 2987 2699
Size: 123 165 6574
Fragments arranged by size:
6574 165 123
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2433 2772 2980 3938 5522 1239
Size: 1194 339 208 958 1584 2579
Fragments arranged by size:
2579 1584 1194 958 339 208
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2487 4987 5852 6150 2487
Size: 2500 865 298 3199
Fragments arranged by size:
3199 2500 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2793 3229 5525 882
Size: 617 1294 436 2296 2219
Fragments arranged by size:
2296 2219 1294 617 436
BsaAI yAC'GTr
Cuts at: 493 1204 4653 493
Size: 711 3449 2702
Fragments arranged by size:
3449 2702 711
BsaBI GATnn'nnATC
Cuts at: 3038 6856 3038
Size: 3818 3044
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4669 5051 5686 275
Size: 53 83 186 936 3136 382 635 1451
Fragments arranged by size:
3136 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5295 5693 5967 6114 6525 5295
Size: 398 274 147 411 5632
Fragments arranged by size:
5632 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2745 2775 2901 2931 2953
Size: 30 165 30 1342 30 126 30 22
Cuts at: 2953 2983 3168 3198 3738 3768 1178
Size: 30 185 30 540 30 4272
Fragments arranged by size:
4272 1342 540 185 165 126 30 30
30 30 30 30 30 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2096 2436 2490 3108 3217 3326 3759 1186
Size: 910 340 54 618 109 109 433 4289
Fragments arranged by size:
4289 910 618 433 340 109 109 54
BseYI C'CCAG_C
Cuts at: 2072 2655 2821 3264 6065 6561 2072
Size: 583 166 443 2801 496 2373
Fragments arranged by size:
2801 2373 583 496 443 166
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2814 4148 1163
Size: 573 1078 1334 3877
Fragments arranged by size:
3877 1334 1078 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4312 5073 6101 1540
Size: 275 186 2311 761 1028 2301
Fragments arranged by size:
2311 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3610 3783 4923 5008 6079 3610
Size: 173 1140 85 1071 4393
Fragments arranged by size:
4393 1140 1071 173 85
BsmI GAATG_Cn'
Cuts at: 2514 4373 4466 2514
Size: 1859 93 4910
Fragments arranged by size:
4910 1859 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2982 3610 3783 4181 4923 5008 6079
Size: 399 1192 628 173 398 742 85 1071
Cuts at: 6079 1391
Size: 2174
Fragments arranged by size:
2174 1192 1071 742 628 399 398 173
85
BspEI T'CCGG_A
Cuts at: 6525 6525
Size: 6862
BspHI T'CATG_A
Cuts at: 3190 4751 6481 3190
Size: 1561 1730 3571
Fragments arranged by size:
3571 1730 1561
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4217 844
Size: 3373 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4749 1394
Size: 429 2926 3507
Fragments arranged by size:
3507 2926 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5357 5531 65
Size: 1183 4109 174 1396
Fragments arranged by size:
4109 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5506 5693 1700
Size: 110 3696 187 2869
Fragments arranged by size:
3696 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3401 3414 96
Size: 3305 13 3544
Fragments arranged by size:
3544 3305 13
BssHII G'CGCG_C
Cuts at: 1925 2048 1925
Size: 123 6739
BssSI C'ACGA_G
Cuts at: 4922 5934 4922
Size: 1012 5850
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6862
BstBI TT'CG_AA
Cuts at: 5666 5666
Size: 6862
BstEII G'GTnAC_C
Cuts at: 1184 2816 3131 1184
Size: 1632 315 4915
Fragments arranged by size:
4915 1632 315
BstXI CCAn_nnnn'nTGG
Cuts at: 2739 3587 2739
Size: 848 6014
BstYI r'GATC_y
Cuts at: 1972 3551 4192 4559 4944 4961 6413 6499 6511
Size: 1579 641 367 385 17 1452 86 12
Cuts at: 6511 6857 1972
Size: 346 1977
Fragments arranged by size:
1977 1579 1452 641 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3615 3615
Size: 6862
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6310
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6123
Fragments arranged by size:
6123 683 56
BtsI GCAGTG_nn'
Cuts at: 3105 4378 5172 5192 3105
Size: 1273 794 20 4775
Fragments arranged by size:
4775 1273 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6862
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2282 2317 529
Size: 35 1718 35 5074
Fragments arranged by size:
5074 1718 35 35
DraI TTT'AAA
Cuts at: 2563 3715 4166 2563
Size: 1152 451 5259
Fragments arranged by size:
5259 1152 451
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3116 5869 809
Size: 507 1800 2753 1802
Fragments arranged by size:
2753 1802 1800 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 4309 5198
Size: 54 1425 50 275 18 210 2269 889
Cuts at: 5198 6827 8
Size: 1629 43
Fragments arranged by size:
2269 1629 1425 889 275 210 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4309 1537
Size: 275 2497 4090
Fragments arranged by size:
4090 2497 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2123 4091 4792 1353
Size: 770 1968 701 3423
Fragments arranged by size:
3423 1968 770 701
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5449 5823 5969 387
Size: 1064 138 3860 374 146 1280
Fragments arranged by size:
3860 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2433 2571 2772 2980 3582 3938 4925
Size: 393 801 138 201 208 602 356 987
Cuts at: 4925 5522 6309 6577 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 987 801 787 602 597 393 356
268 208 201 138
EcoNI CCTnn'n_nnAGG
Cuts at: 2448 3459 2448
Size: 1011 5851
EcoRI G'AATT_C
Cuts at: 3316 4171 4549 3316
Size: 855 378 5629
Fragments arranged by size:
5629 855 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2239 2239
Size: 6862
FspI TGC'GCA
Cuts at: 5368 5368
Size: 6862
HaeII r_GCGC'y
Cuts at: 1678 2081 6009 1678
Size: 403 3928 2531
Fragments arranged by size:
3928 2531 403
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2745 2777 2953 2985 3070
Size: 32 135 32 1173 32 176 32 85
Cuts at: 3070 3102 5503 5535 5577 5609 1373
Size: 32 2401 32 42 32 2626
Fragments arranged by size:
2626 2401 1173 176 135 85 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2536 4206 4452 669
Size: 988 281 598 1670 246 3079
Fragments arranged by size:
3079 1670 988 598 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6862
HpaI GTT'AAC
Cuts at: 2536 4452 2536
Size: 1916 4946
KpnI G_GTAC'C
Cuts at: 4187 4187
Size: 6862
MfeI C'AATT_G
Cuts at: 2587 2892 4461 2587
Size: 305 1569 4988
Fragments arranged by size:
4988 1569 305
MluI A'CGCG_T
Cuts at: 6738 6738
Size: 6862
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2715 2720 2971 3473 3604 5976 6160 1305
Size: 1410 5 251 502 131 2372 184 2007
Fragments arranged by size:
2372 2007 1410 502 251 184 131 5
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2042 10
Size: 54 1425 343 210 4830
Fragments arranged by size:
4830 1425 343 210 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2343 4820 5179 5338 518
Size: 648 1177 2477 359 159 2042
Fragments arranged by size:
2477 2042 1177 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2659 2958 3182 3409 3645 4136 4278 4955
Size: 1053 299 224 227 236 491 142 677
Cuts at: 4955 6103 6348 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1053 677 491 299 245 236
227 224 142
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6310
NdeI CA'TA_TG
Cuts at: 387 3679 387
Size: 3292 3570
NheI G'CTAG_C
Cuts at: 1052 2332 3763 1052
Size: 1280 1431 4151
Fragments arranged by size:
4151 1431 1280
NotI GC'GGCC_GC
Cuts at: 4309 4309
Size: 6862
NspI r_CATG'y
Cuts at: 1132 1229 3532 3980 4220 5765 1132
Size: 97 2303 448 240 1545 2229
Fragments arranged by size:
2303 2229 1545 448 240 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6862
PciI A'CATG_T
Cuts at: 5761 5761
Size: 6862
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4083 1108
Size: 2975 3887
PfoI T'CCnGG_A
Cuts at: 2449 2749 3954 2449
Size: 300 1205 5357
Fragments arranged by size:
5357 1205 300
PmeI GTTT'AAAC
Cuts at: 4166 4166
Size: 6862
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4931 4963 6444 6476 4931
Size: 32 1481 32 5317
Fragments arranged by size:
5317 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6862
PsiI TTA'TAA
Cuts at: 2132 4432 2132
Size: 2300 4562
PstI C_TGCA'G
Cuts at: 830 3910 4141 4214 830
Size: 3080 231 73 3478
Fragments arranged by size:
3478 3080 231 73
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6862
PvuII CAG'CTG
Cuts at: 2659 2958 3182 4136 2659
Size: 299 224 954 5385
Fragments arranged by size:
5385 954 299 224
SalI G'TCGA_C
Cuts at: 1936 4204 1936
Size: 2268 4594
SbfI CC_TGCA'GG
Cuts at: 4214 4214
Size: 6862
ScaI AGT'ACT
Cuts at: 5110 6822 5110
Size: 1712 5150
SexAI A'CCwGG_T
Cuts at: 2812 2812
Size: 6862
SfcI C'TryA_G
Cuts at: 826 1045 3369 3807 3838 3906 4137 4210 5345
Size: 219 2324 438 31 68 231 73 1135
Cuts at: 5345 6026 6217 826
Size: 681 191 1471
Fragments arranged by size:
2324 1471 1135 681 438 231 219 191
73 68 31
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6862
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6862
SmaI CCC'GGG
Cuts at: 1872 4189 1872
Size: 2317 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2502 4966 5867 6129 820
Size: 197 926 559 2464 901 262 1553
Fragments arranged by size:
2464 1553 926 901 559 262 197
SnaBI TAC'GTA
Cuts at: 493 4653 493
Size: 4160 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6862
SphI G_CATG'C
Cuts at: 1132 3532 4220 1132
Size: 2400 688 3774
Fragments arranged by size:
3774 2400 688
SspI AAT'ATT
Cuts at: 5 52 4786 5
Size: 47 4734 2081
Fragments arranged by size:
4734 2081 47
StuI AGG'CCT
Cuts at: 1517 4082 1517
Size: 2565 4297
StyI C'CwwG_G
Cuts at: 513 1065 4077 4301 513
Size: 552 3012 224 3074
Fragments arranged by size:
3074 3012 552 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2921 3136 4889 4906 5059 5244 5689
Size: 1255 1341 215 1753 17 153 185 445
Cuts at: 5689 325
Size: 1498
Fragments arranged by size:
1753 1498 1341 1255 445 215 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3401 3414 5108
Size: 274 80 33 51 1443 1424 13 1694
Cuts at: 5108 6820 96
Size: 1712 138
Fragments arranged by size:
1712 1694 1443 1424 274 138 80 51
33 13
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2901 2933 3738 3770 6822 6854 2901
Size: 32 805 32 3052 32 2909
Fragments arranged by size:
3052 2909 805 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6862
XbaI T'CTAG_A
Cuts at: 4198 4198
Size: 6862
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2955 2955
Size: 6862
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6862
XmaI C'CCGG_G
Cuts at: 1870 4187 1870
Size: 2317 4545
XmnI GAAnn'nnTTC
Cuts at: 2627 3013 3595 4991 2627
Size: 386 582 1396 4498
Fragments arranged by size:
4498 1396 582 386
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4670 276
Size: 53 83 186 936 3136 2468
Fragments arranged by size:
3136 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI