Restriction map for
pFN21AE4566
(Circular) (Six-base) MAPSORT of: pFN21AE4566.seq Check: 7564 from: 1 to: 6835
pFN21AE4566 6835 bp
With 182 enzymes: SgfI *
July 6, 2012 17:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3075 4645 278
Size: 53 83 186 936 1539 1570 2468
Fragments arranged by size:
2468 1570 1539 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4122 4178 1937
Size: 2185 56 4594
Fragments arranged by size:
4594 2185 56
Acc65I G'GTAC_C
Cuts at: 4156 4156
Size: 6835
AclI AA'CG_TT
Cuts at: 1563 4962 5335 1563
Size: 3399 373 3063
Fragments arranged by size:
3399 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2000 2855 2941 2988 3776 3956 3974 4898
Size: 368 855 86 47 788 180 18 924
Cuts at: 4898 6282 6550 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 924 855 788 368 268 180
86 47 18
AflII C'TTAA_G
Cuts at: 820 1017 3119 820
Size: 197 2102 4536
Fragments arranged by size:
4536 2102 197
AflIII A'CryG_T
Cuts at: 1203 2028 4089 5734 6711 1203
Size: 825 2061 1645 977 1327
Fragments arranged by size:
2061 1645 1327 977 825
AgeI A'CCGG_T
Cuts at: 5666 5666
Size: 6835
AhdI GACnn_n'nnGTC
Cuts at: 5564 5564
Size: 6835
AleI CACnn'nnGTG
Cuts at: 1166 3489 1166
Size: 2323 4512
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4028 1184
Size: 2844 3991
AlwNI CAG_nnn'CTG
Cuts at: 4578 6150 4578
Size: 1572 5263
ApaLI G'TGCA_C
Cuts at: 4892 6048 4892
Size: 1156 5679
ApoI r'AATT_y
Cuts at: 1493 2073 4144 4370 4522 6631 1493
Size: 580 2071 226 152 2109 1697
Fragments arranged by size:
2109 2071 1697 580 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3654 5389 160
Size: 872 2622 1735 1606
Fragments arranged by size:
2622 1735 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6835
AvaI C'yCGr_G
Cuts at: 1870 1943 4160 1870
Size: 73 2217 4545
Fragments arranged by size:
4545 2217 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3148 3181 3148
Size: 33 6802
BamHI G'GATC_C
Cuts at: 2962 4165 4532 2962
Size: 1203 367 5265
Fragments arranged by size:
5265 1203 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2483 4110 4156 5611 618
Size: 325 217 627 696 1627 46 1455 1842
Fragments arranged by size:
1842 1627 1455 696 627 325 217 46
BanII G_rGCy'C
Cuts at: 1391 4154 1391
Size: 2763 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2106 928
Size: 971 207 5657
Fragments arranged by size:
5657 971 207
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5026 5060 1903
Size: 34 50 34 3005 34 3678
Fragments arranged by size:
3678 3005 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3506 4727 5937 3506
Size: 1221 1210 4404
Fragments arranged by size:
4404 1221 1210
BclI T'GATC_A
Cuts at: 1548 2943 3615 1548
Size: 1395 672 4768
Fragments arranged by size:
4768 1395 672
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5446 136
Size: 107 122 71 631 605 3774 1525
Fragments arranged by size:
3774 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6830 6830
Size: 6835
BlpI GC'TnA_GC
Cuts at: 4252 4252
Size: 6835
Bme1580I G_kGCm'C
Cuts at: 1790 4896 6052 1790
Size: 3106 1156 2573
Fragments arranged by size:
3106 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2029 3543 1318
Size: 711 1514 4610
Fragments arranged by size:
4610 1514 711
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2461 4566 5524
Size: 416 53 83 399 24 1044 2105 958
Cuts at: 5524 442
Size: 1753
Fragments arranged by size:
2105 1753 1044 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6835
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4099 4099
Size: 6835
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3198 3275 5495 1239
Size: 1959 77 2220 2579
Fragments arranged by size:
2579 2220 1959 77
Bpu10I CC'TnA_GC
Cuts at: 2997 2997
Size: 6835
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2186 4960 5825 6123 2186
Size: 2774 865 298 2898
Fragments arranged by size:
2898 2774 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2036 3697 5498 882
Size: 617 537 1661 1801 2219
Fragments arranged by size:
2219 1801 1661 617 537
BsaAI yAC'GTr
Cuts at: 493 1204 2881 4024 4626 493
Size: 711 1677 1143 602 2702
Fragments arranged by size:
2702 1677 1143 711 602
BsaBI GATnn'nnATC
Cuts at: 6829 6829
Size: 6835
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3072 4642 5024 5659
Size: 53 83 186 936 1539 1570 382 635
Cuts at: 5659 275
Size: 1451
Fragments arranged by size:
1570 1539 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5268 5666 5940 6087 6498 5268
Size: 398 274 147 411 5605
Fragments arranged by size:
5605 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6610
Fragments arranged by size:
6610 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3263 3534 3899 1186
Size: 2077 271 365 4122
Fragments arranged by size:
4122 2077 365 271
BseYI C'CCAG_C
Cuts at: 6038 6534 6038
Size: 496 6339
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6262
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4285 5046 6074 1540
Size: 275 186 2284 761 1028 2301
Fragments arranged by size:
2301 2284 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3115 3809 4896 4981 6052 3115
Size: 694 1087 85 1071 3898
Fragments arranged by size:
3898 1087 1071 694 85
BsmI GAATG_Cn'
Cuts at: 4346 4439 4346
Size: 93 6742
BsmBI CGTCTCn'nnnn_
Cuts at: 3533 3533
Size: 6835
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3115 3809 4154 4896 4981 6052 1391
Size: 399 1325 694 345 742 85 1071 2174
Fragments arranged by size:
2174 1325 1071 742 694 399 345 85
BspEI T'CCGG_A
Cuts at: 6498 6498
Size: 6835
BspHI T'CATG_A
Cuts at: 2469 4724 6454 2469
Size: 2255 1730 2850
Fragments arranged by size:
2850 2255 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4190 844
Size: 3346 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3133 4722 1394
Size: 429 1310 1589 3507
Fragments arranged by size:
3507 1589 1310 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3851 3960 5330 5504 65
Size: 1183 2603 109 1370 174 1396
Fragments arranged by size:
2603 1396 1370 1183 174 109
BsrFI r'CCGG_y
Cuts at: 1700 1810 5479 5666 1700
Size: 110 3669 187 2869
Fragments arranged by size:
3669 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2308 96
Size: 2212 4623
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6835
BssSI C'ACGA_G
Cuts at: 4895 5907 4895
Size: 1012 5823
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6835
BstBI TT'CG_AA
Cuts at: 5639 5639
Size: 6835
BstEII G'GTnAC_C
Cuts at: 1184 3277 1184
Size: 2093 4742
BstXI CCAn_nnnn'nTGG
Cuts at: 3049 3049
Size: 6835
BstYI r'GATC_y
Cuts at: 1972 2962 4165 4532 4917 4934 6386 6472 6484
Size: 990 1203 367 385 17 1452 86 12
Cuts at: 6484 6830 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1203 990 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 4042 513
Size: 552 2977 3306
Fragments arranged by size:
3306 2977 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2806 4087 487
Size: 683 56 1580 1281 3235
Fragments arranged by size:
3235 1580 1281 683 56
BtsI GCAGTG_nn'
Cuts at: 2177 3510 3912 4351 5145 5165 2177
Size: 1333 402 439 794 20 3847
Fragments arranged by size:
3847 1333 794 439 402 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6835
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6800
DraI TTT'AAA
Cuts at: 4139 4139
Size: 6835
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3935 5842 809
Size: 507 2619 1907 1802
Fragments arranged by size:
2619 1907 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2734 3507 3564
Size: 54 1425 50 275 18 904 773 57
Cuts at: 3564 3778 4282 5171 6800 8
Size: 214 504 889 1629 43
Fragments arranged by size:
1629 1425 904 889 773 504 275 214
57 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4282 1537
Size: 275 2470 4090
Fragments arranged by size:
4090 2470 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2964 4765 1353
Size: 1611 1801 3423
Fragments arranged by size:
3423 1801 1611
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5422 5796 5942 387
Size: 1064 138 3833 374 146 1280
Fragments arranged by size:
3833 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2000 2855 2941 2988 3198 3275 3776
Size: 393 368 855 86 47 210 77 501
Cuts at: 3776 3956 3974 4898 5495 6282 6550 1239
Size: 180 18 924 597 787 268 1524
Fragments arranged by size:
1524 924 855 787 597 501 393 368
268 210 180 86 77 47 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2804 2804
Size: 6835
EcoRI G'AATT_C
Cuts at: 4144 4522 4144
Size: 378 6457
FspI TGC'GCA
Cuts at: 5341 5341
Size: 6835
HaeII r_GCGC'y
Cuts at: 1678 5982 1678
Size: 4304 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3696 3728 5476 5508 5550
Size: 32 135 32 2124 32 1748 32 42
Cuts at: 5550 5582 1373
Size: 32 2626
Fragments arranged by size:
2626 2124 1748 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4123 4179 4425 669
Size: 988 281 2185 56 246 3079
Fragments arranged by size:
3079 2185 988 281 246 56
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6835
HpaI GTT'AAC
Cuts at: 4425 4425
Size: 6835
KpnI G_GTAC'C
Cuts at: 4160 4160
Size: 6835
MfeI C'AATT_G
Cuts at: 4434 4434
Size: 6835
MluI A'CGCG_T
Cuts at: 6711 6711
Size: 6835
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3066 3718 5949 6133 1305
Size: 1761 652 2231 184 2007
Fragments arranged by size:
2231 2007 1761 652 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3509 3566 10
Size: 54 1425 343 1677 57 3279
Fragments arranged by size:
3279 1677 1425 343 57 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3489 3552 4793 5152 5311 518
Size: 648 2323 63 1241 359 159 2042
Fragments arranged by size:
2323 2042 1241 648 359 159 63
MspA1I CmG'CkG
Cuts at: 1606 2526 3162 4251 4928 6076 6321 1606
Size: 920 636 1089 677 1148 245 2120
Fragments arranged by size:
2120 1148 1089 920 677 636 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6283
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6835
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6835
NotI GC'GGCC_GC
Cuts at: 4282 4282
Size: 6835
NspI r_CATG'y
Cuts at: 1132 1229 3754 4093 4193 5738 1132
Size: 97 2525 339 100 1545 2229
Fragments arranged by size:
2525 2229 1545 339 100 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6835
PciI A'CATG_T
Cuts at: 4089 5734 4089
Size: 1645 5190
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2647 1108
Size: 1539 5296
PfoI T'CCnGG_A
Cuts at: 2343 2343
Size: 6835
PmeI GTTT'AAAC
Cuts at: 4139 4139
Size: 6835
PmlI CAC'GTG
Cuts at: 4024 4024
Size: 6835
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4904 4936 6417 6449 4904
Size: 32 1481 32 5290
Fragments arranged by size:
5290 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6835
PsiI TTA'TAA
Cuts at: 4405 4405
Size: 6835
PstI C_TGCA'G
Cuts at: 830 3090 4187 830
Size: 2260 1097 3478
Fragments arranged by size:
3478 2260 1097
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6835
PvuII CAG'CTG
Cuts at: 2526 2526
Size: 6835
SalI G'TCGA_C
Cuts at: 1936 4121 4177 1936
Size: 2185 56 4594
Fragments arranged by size:
4594 2185 56
SbfI CC_TGCA'GG
Cuts at: 4187 4187
Size: 6835
ScaI AGT'ACT
Cuts at: 2988 5083 6795 2988
Size: 2095 1712 3028
Fragments arranged by size:
3028 2095 1712
SfcI C'TryA_G
Cuts at: 826 1045 2801 3086 3454 4183 5318 5999 6190
Size: 219 1756 285 368 729 1135 681 191
Cuts at: 6190 826
Size: 1471
Fragments arranged by size:
1756 1471 1135 729 681 368 285 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6835
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6835
SmaI CCC'GGG
Cuts at: 1872 4162 1872
Size: 2290 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2165 3119 4939 5840 6102 820
Size: 197 926 222 954 1820 901 262 1553
Fragments arranged by size:
1820 1553 954 926 901 262 222 197
SnaBI TAC'GTA
Cuts at: 493 4626 493
Size: 4133 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6835
SphI G_CATG'C
Cuts at: 1132 4193 1132
Size: 3061 3774
SspI AAT'ATT
Cuts at: 5 52 2417 4759 5
Size: 47 2365 2342 2081
Fragments arranged by size:
2365 2342 2081 47
StuI AGG'CCT
Cuts at: 1517 2126 3675 1517
Size: 609 1549 4677
Fragments arranged by size:
4677 1549 609
StyI C'CwwG_G
Cuts at: 513 1065 4032 4274 513
Size: 552 2967 242 3074
Fragments arranged by size:
3074 2967 552 242
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2443 2699 3463 3479 3792 4862 4879
Size: 1255 863 256 764 16 313 1070 17
Cuts at: 4879 5032 5217 5662 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1070 863 764 445 313 256
185 153 17 16
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2308 2986 3975
Size: 274 80 33 51 1443 331 678 989
Cuts at: 3975 5081 6793 96
Size: 1106 1712 138
Fragments arranged by size:
1712 1443 1106 989 678 331 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3016 3048 6795 6827 3016
Size: 32 3747 32 3024
Fragments arranged by size:
3747 3024 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6835
XbaI T'CTAG_A
Cuts at: 4171 4171
Size: 6835
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2341 3250 2341
Size: 909 5926
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6835
XmaI C'CCGG_G
Cuts at: 1870 4160 1870
Size: 2290 4545
XmnI GAAnn'nnTTC
Cuts at: 4964 4964
Size: 6835
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3073 4643 276
Size: 53 83 186 936 1539 1570 2468
Fragments arranged by size:
2468 1570 1539 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI