Restriction map for
pFN21AE4565
(Circular) (Six-base) MAPSORT of: pFN21AE4565.seq Check: 9042 from: 1 to: 6811
pFN21AE4565 6811 bp
With 182 enzymes: SgfI *
March 28, 2012 14:16 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4621 278
Size: 53 83 186 936 3085 2468
Fragments arranged by size:
3085 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2823 3387 3503 4154 1937
Size: 886 564 116 651 4594
Fragments arranged by size:
4594 886 651 564 116
Acc65I G'GTAC_C
Cuts at: 4132 4132
Size: 6811
AclI AA'CG_TT
Cuts at: 1563 4938 5311 1563
Size: 3375 373 3063
Fragments arranged by size:
3375 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2823 4073 4874 6258 6526 1632
Size: 1191 1250 801 1384 268 1917
Fragments arranged by size:
1917 1384 1250 1191 801 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6614
AflIII A'CryG_T
Cuts at: 1203 5710 6687 1203
Size: 4507 977 1327
Fragments arranged by size:
4507 1327 977
AgeI A'CCGG_T
Cuts at: 5642 5642
Size: 6811
AhdI GACnn_n'nnGTC
Cuts at: 5540 5540
Size: 6811
AleI CACnn'nnGTG
Cuts at: 1166 2244 1166
Size: 1078 5733
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6811
AlwNI CAG_nnn'CTG
Cuts at: 3991 4554 6126 3991
Size: 563 1572 4676
Fragments arranged by size:
4676 1572 563
ApaI G_GGCC'C
Cuts at: 2281 2281
Size: 6811
ApaLI G'TGCA_C
Cuts at: 4868 6024 4868
Size: 1156 5655
ApoI r'AATT_y
Cuts at: 1493 2166 2598 2619 2887 3629 3938 4120 4346
Size: 673 432 21 268 742 309 182 226
Cuts at: 4346 4498 6607 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 742 673 432 309 268 226
182 152 21
AseI AT'TA_AT
Cuts at: 160 1032 5365 160
Size: 872 4333 1606
Fragments arranged by size:
4333 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6811
AvaI C'yCGr_G
Cuts at: 1870 1943 2036 2281 4136 1870
Size: 73 93 245 1855 4545
Fragments arranged by size:
4545 1855 245 93 73
BamHI G'GATC_C
Cuts at: 2420 2539 4141 4508 2420
Size: 119 1602 367 4723
Fragments arranged by size:
4723 1602 367 119
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4132 5587 618
Size: 325 217 627 2345 1455 1842
Fragments arranged by size:
2345 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2281 4130 1391
Size: 890 1849 4072
Fragments arranged by size:
4072 1849 890
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3369 3857 928
Size: 971 1470 488 3882
Fragments arranged by size:
3882 1470 971 488
BbvCI CC'TCA_GC
Cuts at: 3237 3237
Size: 6811
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5002 5036 1903
Size: 34 3065 34 3678
Fragments arranged by size:
3678 3065 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2523 2690 4703 5913 2523
Size: 167 2013 1210 3421
Fragments arranged by size:
3421 2013 1210 167
BclI T'GATC_A
Cuts at: 1548 3445 1548
Size: 1897 4914
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2069 5422
Size: 107 122 71 631 605 337 60 3353
Cuts at: 5422 136
Size: 1525
Fragments arranged by size:
3353 1525 631 605 337 122 107 71
60
BglII A'GATC_T
Cuts at: 2460 6806 2460
Size: 4346 2465
BlpI GC'TnA_GC
Cuts at: 4228 4228
Size: 6811
Bme1580I G_kGCm'C
Cuts at: 1790 2082 2281 4872 6028 1790
Size: 292 199 2591 1156 2573
Fragments arranged by size:
2591 2573 1156 292 199
BmgBI CAC'GTC
Cuts at: 1318 2077 1318
Size: 759 6052
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2545 4542 5500
Size: 416 53 83 399 24 1128 1997 958
Cuts at: 5500 442
Size: 1753
Fragments arranged by size:
1997 1753 1128 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6811
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4097 4097
Size: 6811
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2120 2328 2977 3519 5471 1239
Size: 881 208 649 542 1952 2579
Fragments arranged by size:
2579 1952 881 649 542 208
Bpu10I CC'TnA_GC
Cuts at: 3237 3237
Size: 6811
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4936 5801 6099 4936
Size: 865 298 5648
Fragments arranged by size:
5648 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5474 882
Size: 617 3975 2219
Fragments arranged by size:
3975 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3814 4602 493
Size: 711 2610 788 2702
Fragments arranged by size:
2702 2610 788 711
BsaBI GATnn'nnATC
Cuts at: 2720 6805 2720
Size: 4085 2726
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2235 4039 4618 5000
Size: 53 83 186 936 702 1804 579 382
Cuts at: 5000 5635 275
Size: 635 1451
Fragments arranged by size:
1804 1451 936 702 635 579 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5244 5642 5916 6063 6474 5244
Size: 398 274 147 411 5581
Fragments arranged by size:
5581 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6586
Fragments arranged by size:
6586 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6811
BseYI C'CCAG_C
Cuts at: 2997 6014 6510 2997
Size: 3017 496 3298
Fragments arranged by size:
3298 3017 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6238
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4261 5022 6050 1540
Size: 275 186 2260 761 1028 2301
Fragments arranged by size:
2301 2260 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3269 4872 4957 6028 3269
Size: 1603 85 1071 4052
Fragments arranged by size:
4052 1603 1071 85
BsmI GAATG_Cn'
Cuts at: 2566 2706 3258 4322 4415 2566
Size: 140 552 1064 93 4962
Fragments arranged by size:
4962 1064 552 140 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2082 2281 3269 4130 4872 4957 6028
Size: 399 292 199 988 861 742 85 1071
Cuts at: 6028 1391
Size: 2174
Fragments arranged by size:
2174 1071 988 861 742 399 292 199
85
BspEI T'CCGG_A
Cuts at: 6474 6474
Size: 6811
BspHI T'CATG_A
Cuts at: 2959 4700 6430 2959
Size: 1741 1730 3340
Fragments arranged by size:
3340 1741 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2136 3851 4166 844
Size: 1292 1715 315 3489
Fragments arranged by size:
3489 1715 1292 315
BsrBI CCG'CTC
Cuts at: 1394 1823 4698 1394
Size: 429 2875 3507
Fragments arranged by size:
3507 2875 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2674 3423 3843 3926 5306 5480 65
Size: 1183 1426 749 420 83 1380 174 1396
Fragments arranged by size:
1426 1396 1380 1183 749 420 174 83
BsrFI r'CCGG_y
Cuts at: 1700 1810 5455 5642 1700
Size: 110 3645 187 2869
Fragments arranged by size:
3645 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4059 96
Size: 3963 2848
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6811
BssSI C'ACGA_G
Cuts at: 2408 4871 5883 2408
Size: 2463 1012 3336
Fragments arranged by size:
3336 2463 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6811
BstBI TT'CG_AA
Cuts at: 5615 5615
Size: 6811
BstEII G'GTnAC_C
Cuts at: 1184 2753 1184
Size: 1569 5242
BstXI CCAn_nnnn'nTGG
Cuts at: 2098 3330 2098
Size: 1232 5579
BstYI r'GATC_y
Cuts at: 1972 2420 2460 2539 4099 4141 4508 4893 4910
Size: 448 40 79 1560 42 367 385 17
Cuts at: 4910 6362 6448 6460 6806 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1560 1452 448 385 367 346 86
79 42 40 17 12
BstZ17I GTA'TAC
Cuts at: 3388 3504 3388
Size: 116 6695
BtgI C'CryG_G
Cuts at: 513 1065 2004 2025 2130 2480 2585 2838 3103
Size: 552 939 21 105 350 105 253 265
Cuts at: 3103 3312 513
Size: 209 4012
Fragments arranged by size:
4012 939 552 350 265 253 209 105
105 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6072
Fragments arranged by size:
6072 683 56
BtsI GCAGTG_nn'
Cuts at: 4327 5121 5141 4327
Size: 794 20 5997
Fragments arranged by size:
5997 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6811
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2475 2510 4056 4091 529
Size: 35 1911 35 1546 35 3249
Fragments arranged by size:
3249 1911 1546 35 35 35
DraI TTT'AAA
Cuts at: 3355 4115 3355
Size: 760 6051
DraIII CAC_nnn'GTG
Cuts at: 3213 3213
Size: 6811
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5818 809
Size: 507 4502 1802
Fragments arranged by size:
4502 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2088 4258 5147
Size: 54 1425 50 275 18 258 2170 889
Cuts at: 5147 6776 8
Size: 1629 43
Fragments arranged by size:
2170 1629 1425 889 275 258 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4258 1537
Size: 275 2446 4090
Fragments arranged by size:
4090 2446 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2799 4741 1353
Size: 1446 1942 3423
Fragments arranged by size:
3423 1942 1446
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5398 5772 5918 387
Size: 1064 138 3809 374 146 1280
Fragments arranged by size:
3809 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2120 2328 2823 2977 3519 4073 4874
Size: 393 488 208 495 154 542 554 801
Cuts at: 4874 5471 6258 6526 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 801 787 597 554 542 495 488
393 268 208 154
EcoO109I rG'GnC_Cy
Cuts at: 2278 2278
Size: 6811
EcoRI G'AATT_C
Cuts at: 4120 4498 4120
Size: 378 6433
FspI TGC'GCA
Cuts at: 5317 5317
Size: 6811
HaeII r_GCGC'y
Cuts at: 1678 2057 3409 5958 1678
Size: 379 1352 2549 2531
Fragments arranged by size:
2549 2531 1352 379
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2452 2484 2947 2979 3230
Size: 32 135 32 880 32 463 32 251
Cuts at: 3230 3262 3430 3462 5452 5484 5526 5558 1373
Size: 32 168 32 1990 32 42 32 2626
Fragments arranged by size:
2626 1990 880 463 251 168 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4155 4401 669
Size: 988 281 2217 246 3079
Fragments arranged by size:
3079 2217 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2767 748
Size: 2019 4792
HpaI GTT'AAC
Cuts at: 4401 4401
Size: 6811
KpnI G_GTAC'C
Cuts at: 4136 4136
Size: 6811
MfeI C'AATT_G
Cuts at: 4410 4410
Size: 6811
MluI A'CGCG_T
Cuts at: 6687 6687
Size: 6811
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2303 3021 3898 5925 6109 1305
Size: 998 718 877 2027 184 2007
Fragments arranged by size:
2027 2007 998 877 718 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2090 10
Size: 54 1425 343 258 4731
Fragments arranged by size:
4731 1425 343 258 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2244 2553 2874 3102 4769 5128 5287
Size: 648 1078 309 321 228 1667 359 159
Cuts at: 5287 518
Size: 2042
Fragments arranged by size:
2042 1667 1078 648 359 321 309 228
159
MspA1I CmG'CkG
Cuts at: 1606 2027 2997 3968 4227 4904 6052 6297 1606
Size: 421 970 971 259 677 1148 245 2120
Fragments arranged by size:
2120 1148 971 970 677 421 259 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2838 3103 3312 513
Size: 552 939 834 265 209 4012
Fragments arranged by size:
4012 939 834 552 265 209
NdeI CA'TA_TG
Cuts at: 387 2554 387
Size: 2167 4644
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6811
NotI GC'GGCC_GC
Cuts at: 4258 4258
Size: 6811
NspI r_CATG'y
Cuts at: 1132 1229 2667 2735 4169 5714 1132
Size: 97 1438 68 1434 1545 2229
Fragments arranged by size:
2229 1545 1438 1434 97 68
PasI CC'CwG_GG
Cuts at: 1348 3280 1348
Size: 1932 4879
PciI A'CATG_T
Cuts at: 5710 5710
Size: 6811
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3109 1108
Size: 2001 4810
PfoI T'CCnGG_A
Cuts at: 2097 2097
Size: 6811
PmeI GTTT'AAAC
Cuts at: 4115 4115
Size: 6811
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4880 4912 6393 6425 4880
Size: 32 1481 32 5266
Fragments arranged by size:
5266 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6811
PsiI TTA'TAA
Cuts at: 2652 4381 2652
Size: 1729 5082
PspOMI G'GGCC_C
Cuts at: 2277 2277
Size: 6811
PstI C_TGCA'G
Cuts at: 830 4163 830
Size: 3333 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6811
PvuII CAG'CTG
Cuts at: 2997 2997
Size: 6811
SacII CC_GC'GG
Cuts at: 2028 2028
Size: 6811
SalI G'TCGA_C
Cuts at: 1936 4153 1936
Size: 2217 4594
SapI GCTCTTCn'nnn_
Cuts at: 2799 2799
Size: 6811
SbfI CC_TGCA'GG
Cuts at: 4163 4163
Size: 6811
ScaI AGT'ACT
Cuts at: 5059 6771 5059
Size: 1712 5099
SfcI C'TryA_G
Cuts at: 826 1045 4159 5294 5975 6166 826
Size: 219 3114 1135 681 191 1471
Fragments arranged by size:
3114 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6811
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6811
SmaI CCC'GGG
Cuts at: 1872 2283 4138 1872
Size: 411 1855 4545
Fragments arranged by size:
4545 1855 411
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4915 5816 6078 820
Size: 197 926 2972 901 262 1553
Fragments arranged by size:
2972 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4602 493
Size: 4109 2702
SpeI A'CTAG_T
Cuts at: 152 2444 152
Size: 2292 4519
SphI G_CATG'C
Cuts at: 1132 2735 4169 1132
Size: 1603 1434 3774
Fragments arranged by size:
3774 1603 1434
SspI AAT'ATT
Cuts at: 5 52 3143 4735 5
Size: 47 3091 1592 2081
Fragments arranged by size:
3091 2081 1592 47
StuI AGG'CCT
Cuts at: 1517 2691 3809 1517
Size: 1174 1118 4519
Fragments arranged by size:
4519 1174 1118
StyI C'CwwG_G
Cuts at: 513 1065 2004 2692 2838 3103 3312 4250 513
Size: 552 939 688 146 265 209 938 3074
Fragments arranged by size:
3074 939 938 688 552 265 209 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4838 4855 5008 5193 5638 325
Size: 1255 3258 17 153 185 445 1498
Fragments arranged by size:
3258 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2404 2671 4059
Size: 274 80 33 51 1443 427 267 1388
Cuts at: 4059 5057 6769 96
Size: 998 1712 138
Fragments arranged by size:
1712 1443 1388 998 427 274 267 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3199 3231 6771 6803 3199
Size: 32 3540 32 3207
Fragments arranged by size:
3540 3207 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6811
XbaI T'CTAG_A
Cuts at: 4147 4147
Size: 6811
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3287 3287
Size: 6811
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6811
XmaI C'CCGG_G
Cuts at: 1870 2281 4136 1870
Size: 411 1855 4545
Fragments arranged by size:
4545 1855 411
XmnI GAAnn'nnTTC
Cuts at: 2623 4940 2623
Size: 2317 4494
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4619 276
Size: 53 83 186 936 3085 2468
Fragments arranged by size:
3085 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI Bsu36I EcoICRI EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspXI PsrI RsrII SacI SanDI SexAI
SfiI SfoI SrfI SwaI