Restriction map for
pFN21AE4564
(Circular) (Six-base) MAPSORT of: pFN21AE4564.seq Check: 2304 from: 1 to: 6811
pFN21AE4564 6811 bp
With 182 enzymes: SgfI *
January 12, 2017 12:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2809 3343 2809
Size: 534 6277
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4621 278
Size: 53 83 186 936 3085 2468
Fragments arranged by size:
3085 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2424 4154 1937
Size: 487 1730 4594
Fragments arranged by size:
4594 1730 487
Acc65I G'GTAC_C
Cuts at: 4132 4132
Size: 6811
AclI AA'CG_TT
Cuts at: 1563 4938 5311 1563
Size: 3375 373 3063
Fragments arranged by size:
3375 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4874 6258 6526 1632
Size: 3242 1384 268 1917
Fragments arranged by size:
3242 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6614
AflIII A'CryG_T
Cuts at: 1203 2371 5710 6687 1203
Size: 1168 3339 977 1327
Fragments arranged by size:
3339 1327 1168 977
AgeI A'CCGG_T
Cuts at: 5642 5642
Size: 6811
AhdI GACnn_n'nnGTC
Cuts at: 5540 5540
Size: 6811
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6811
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6811
AlwNI CAG_nnn'CTG
Cuts at: 2921 3494 4028 4554 6126 2921
Size: 573 534 526 1572 3606
Fragments arranged by size:
3606 1572 573 534 526
ApaI G_GGCC'C
Cuts at: 2283 2283
Size: 6811
ApaLI G'TGCA_C
Cuts at: 4868 6024 4868
Size: 1156 5655
ApoI r'AATT_y
Cuts at: 1493 4120 4346 4498 6607 1493
Size: 2627 226 152 2109 1697
Fragments arranged by size:
2627 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5365 160
Size: 872 4333 1606
Fragments arranged by size:
4333 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6811
AvaI C'yCGr_G
Cuts at: 1870 1943 4136 1870
Size: 73 2193 4545
Fragments arranged by size:
4545 2193 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2197 2230 2197
Size: 33 6778
BamHI G'GATC_C
Cuts at: 2888 4141 4508 2888
Size: 1253 367 5191
Fragments arranged by size:
5191 1253 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4132 5587 618
Size: 325 217 627 2345 1455 1842
Fragments arranged by size:
2345 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2283 2856 3390 4130 1391
Size: 892 573 534 740 4072
Fragments arranged by size:
4072 892 740 573 534
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5840
BbvCI CC'TCA_GC
Cuts at: 3302 3302
Size: 6811
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5002 5036 1903
Size: 34 3065 34 3678
Fragments arranged by size:
3678 3065 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3891 4703 5913 3891
Size: 812 1210 4789
Fragments arranged by size:
4789 1210 812
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6811
BfrBI ATG'CAT
Cuts at: 2318 2318
Size: 6811
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5422 136
Size: 107 122 71 631 605 3750 1525
Fragments arranged by size:
3750 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2386 6806 2386
Size: 4420 2391
BlpI GC'TnA_GC
Cuts at: 4228 4228
Size: 6811
Bme1580I G_kGCm'C
Cuts at: 1790 2283 4872 6028 1790
Size: 493 2589 1156 2573
Fragments arranged by size:
2589 2573 1156 493
BmgBI CAC'GTC
Cuts at: 1318 2374 1318
Size: 1056 5755
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2657 3215 3623
Size: 416 53 83 399 24 1240 558 408
Cuts at: 3623 3749 4542 5500 442
Size: 126 793 958 1753
Fragments arranged by size:
1753 1240 958 793 558 416 408 399
126 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6811
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2032 3851 2032
Size: 1819 4992
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2437 3505 5471 1239
Size: 1198 1068 1966 2579
Fragments arranged by size:
2579 1966 1198 1068
Bpu10I CC'TnA_GC
Cuts at: 3302 3302
Size: 6811
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4936 5801 6099 4936
Size: 865 298 5648
Fragments arranged by size:
5648 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4055 5474 882
Size: 617 2556 1419 2219
Fragments arranged by size:
2556 2219 1419 617
BsaAI yAC'GTr
Cuts at: 493 1204 2898 4602 493
Size: 711 1694 1704 2702
Fragments arranged by size:
2702 1704 1694 711
BsaBI GATnn'nnATC
Cuts at: 6805 6805
Size: 6811
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4618 5000 5635 275
Size: 53 83 186 936 3085 382 635 1451
Fragments arranged by size:
3085 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5244 5642 5916 6063 6474 5244
Size: 398 274 147 411 5581
Fragments arranged by size:
5581 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4077 4107 1178
Size: 30 165 30 2674 30 3882
Fragments arranged by size:
3882 2674 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6811
BseYI C'CCAG_C
Cuts at: 2181 4058 6014 6510 2181
Size: 1877 1956 496 2482
Fragments arranged by size:
2482 1956 1877 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2787 3321 1163
Size: 573 1051 534 4653
Fragments arranged by size:
4653 1051 573 534
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4261 5022 6050 1540
Size: 275 186 2260 761 1028 2301
Fragments arranged by size:
2301 2260 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2856 3390 4872 4957 6028 2856
Size: 534 1482 85 1071 3639
Fragments arranged by size:
3639 1482 1071 534 85
BsmI GAATG_Cn'
Cuts at: 4322 4415 4322
Size: 93 6718
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2283 2856 3390 4130 4872 4957 6028
Size: 399 493 573 534 740 742 85 1071
Cuts at: 6028 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 740 573 534 493 399
85
BspEI T'CCGG_A
Cuts at: 6474 6474
Size: 6811
BspHI T'CATG_A
Cuts at: 4077 4700 6430 4077
Size: 623 1730 4458
Fragments arranged by size:
4458 1730 623
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2809 3343 3440 4166 844
Size: 1965 534 97 726 3489
Fragments arranged by size:
3489 1965 726 534 97
BsrBI CCG'CTC
Cuts at: 1394 1823 4698 1394
Size: 429 2875 3507
Fragments arranged by size:
3507 2875 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5306 5480 65
Size: 1183 4058 174 1396
Fragments arranged by size:
4058 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5455 5642 1700
Size: 110 3645 187 2869
Fragments arranged by size:
3645 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6811
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6811
BssSI C'ACGA_G
Cuts at: 4871 5883 4871
Size: 1012 5799
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6811
BstBI TT'CG_AA
Cuts at: 5615 5615
Size: 6811
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6811
BstXI CCAn_nnnn'nTGG
Cuts at: 2473 3007 3541 2473
Size: 534 534 5743
Fragments arranged by size:
5743 534 534
BstYI r'GATC_y
Cuts at: 1972 2386 2888 4141 4508 4893 4910 6362 6448
Size: 414 502 1253 367 385 17 1452 86
Cuts at: 6448 6460 6806 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1253 502 414 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 2425 2425
Size: 6811
Bsu36I CC'TnA_GG
Cuts at: 3579 3579
Size: 6811
BtgI C'CryG_G
Cuts at: 513 1065 2004 2472 3006 3540 513
Size: 552 939 468 534 534 3784
Fragments arranged by size:
3784 939 552 534 534 468
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6072
Fragments arranged by size:
6072 683 56
BtsI GCAGTG_nn'
Cuts at: 2100 2686 3754 4327 5121 5141 2100
Size: 586 1068 573 794 20 3770
Fragments arranged by size:
3770 1068 794 586 573 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6811
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6776
DraI TTT'AAA
Cuts at: 4115 4115
Size: 6811
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5818 809
Size: 507 4502 1802
Fragments arranged by size:
4502 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2410 3457 3991
Size: 54 1425 50 275 18 580 1047 534
Cuts at: 3991 4258 5147 6776 8
Size: 267 889 1629 43
Fragments arranged by size:
1629 1425 1047 889 580 534 275 267
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4258 1537
Size: 275 2446 4090
Fragments arranged by size:
4090 2446 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4741 1353
Size: 3388 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5398 5772 5918 387
Size: 1064 138 3809 374 146 1280
Fragments arranged by size:
3809 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2854 3388 2854
Size: 534 6277
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2437 3505 4874 5471 6258 6526 1239
Size: 393 805 1068 1369 597 787 268 1524
Fragments arranged by size:
1524 1369 1068 805 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2050 2050
Size: 6811
EcoO109I rG'GnC_Cy
Cuts at: 2279 2598 3132 3170 3666 3957 2279
Size: 319 534 38 496 291 5133
Fragments arranged by size:
5133 534 496 319 291 38
EcoRI G'AATT_C
Cuts at: 4120 4498 4120
Size: 378 6433
FspI TGC'GCA
Cuts at: 5317 5317
Size: 6811
HaeII r_GCGC'y
Cuts at: 1678 5958 1678
Size: 4280 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2025 2057 2590 2622 3124
Size: 32 135 32 453 32 533 32 502
Cuts at: 3124 3156 3658 3690 5452 5484 5526 5558 1373
Size: 32 502 32 1762 32 42 32 2626
Fragments arranged by size:
2626 1762 533 502 502 453 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3208 4155 4401 669
Size: 988 281 1270 947 246 3079
Fragments arranged by size:
3079 1270 988 947 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6811
HpaI GTT'AAC
Cuts at: 4401 4401
Size: 6811
KpnI G_GTAC'C
Cuts at: 4136 4136
Size: 6811
MfeI C'AATT_G
Cuts at: 4410 4410
Size: 6811
MluI A'CGCG_T
Cuts at: 6687 6687
Size: 6811
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3024 3423 5925 6109 1305
Size: 1719 399 2502 184 2007
Fragments arranged by size:
2502 2007 1719 399 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2412 3459 10
Size: 54 1425 343 580 1047 3362
Fragments arranged by size:
3362 1425 1047 580 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2873 4769 5128 5287 518
Size: 648 1707 1896 359 159 2042
Fragments arranged by size:
2042 1896 1707 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2202 2581 3115 3420 3649 4058 4227 4904
Size: 596 379 534 305 229 409 169 677
Cuts at: 4904 6052 6297 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 596 534 409 379 305
245 229 169
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5320
Fragments arranged by size:
5320 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6811
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6811
NotI GC'GGCC_GC
Cuts at: 4258 4258
Size: 6811
NsiI A_TGCA'T
Cuts at: 2320 2320
Size: 6811
NspI r_CATG'y
Cuts at: 1132 1229 4169 5714 1132
Size: 97 2940 1545 2229
Fragments arranged by size:
2940 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2276 1348
Size: 928 5883
PciI A'CATG_T
Cuts at: 5710 5710
Size: 6811
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6811
PmeI GTTT'AAAC
Cuts at: 4115 4115
Size: 6811
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4880 4912 6393 6425 4880
Size: 32 1481 32 5266
Fragments arranged by size:
5266 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2598 3132 3170 3666 3957 2598
Size: 534 38 496 291 5452
Fragments arranged by size:
5452 534 496 291 38
PshAI GACnn'nnGTC
Cuts at: 1613 2939 3473 1613
Size: 1326 534 4951
Fragments arranged by size:
4951 1326 534
PsiI TTA'TAA
Cuts at: 4381 4381
Size: 6811
PspOMI G'GGCC_C
Cuts at: 2279 2279
Size: 6811
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3047 3079 3581 3613 3047
Size: 32 502 32 6245
Fragments arranged by size:
6245 502 32 32
PstI C_TGCA'G
Cuts at: 830 2580 3114 3648 4163 830
Size: 1750 534 534 515 3478
Fragments arranged by size:
3478 1750 534 534 515
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6811
PvuII CAG'CTG
Cuts at: 2202 2581 3115 3649 4058 2202
Size: 379 534 534 409 4955
Fragments arranged by size:
4955 534 534 409 379
SacI G_AGCT'C
Cuts at: 2856 3390 2856
Size: 534 6277
SalI G'TCGA_C
Cuts at: 1936 4153 1936
Size: 2217 4594
SbfI CC_TGCA'GG
Cuts at: 4163 4163
Size: 6811
ScaI AGT'ACT
Cuts at: 2810 5059 6771 2810
Size: 2249 1712 2850
Fragments arranged by size:
2850 2249 1712
SfcI C'TryA_G
Cuts at: 826 1045 2197 2576 3024 3110 3471 3644 4159
Size: 219 1152 379 448 86 361 173 515
Cuts at: 4159 5294 5975 6166 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1152 1135 681 515 448 379 361
219 191 173 86
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6811
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6811
SmaI CCC'GGG
Cuts at: 1872 4138 1872
Size: 2266 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4915 5816 6078 820
Size: 197 926 2972 901 262 1553
Fragments arranged by size:
2972 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4602 493
Size: 4109 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6811
SphI G_CATG'C
Cuts at: 1132 4169 1132
Size: 3037 3774
SspI AAT'ATT
Cuts at: 5 52 4735 5
Size: 47 4683 2081
Fragments arranged by size:
4683 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6811
StyI C'CwwG_G
Cuts at: 513 1065 2004 4250 513
Size: 552 939 2246 3074
Fragments arranged by size:
3074 2246 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2861 2986 4838 4855 5008 5193 5638
Size: 1255 1281 125 1852 17 153 185 445
Cuts at: 5638 325
Size: 1498
Fragments arranged by size:
1852 1498 1281 1255 445 185 153 125
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2808 5057 6769
Size: 274 80 33 51 1443 831 2249 1712
Cuts at: 6769 96
Size: 138
Fragments arranged by size:
2249 1712 1443 831 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6771 6803 6771
Size: 32 6779
Tth111I GACn'n_nGTC
Cuts at: 1316 3221 3755 1316
Size: 1905 534 4372
Fragments arranged by size:
4372 1905 534
XbaI T'CTAG_A
Cuts at: 4147 4147
Size: 6811
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2189 2189
Size: 6811
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6811
XmaI C'CCGG_G
Cuts at: 1870 4136 1870
Size: 2266 4545
XmnI GAAnn'nnTTC
Cuts at: 4940 4940
Size: 6811
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4619 276
Size: 53 83 186 936 3085 2468
Fragments arranged by size:
3085 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BsiWI BsmBI DraIII
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PspXI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI