Restriction map for
pFN21AE4562
(Circular) (Six-base) MAPSORT of: pFN21AE4562.seq Check: 9989 from: 1 to: 6772
pFN21AE4562 6772 bp
With 182 enzymes: SgfI *
March 28, 2012 14:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3082 3800 3082
Size: 718 6054
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4582 278
Size: 53 83 186 936 3046 2468
Fragments arranged by size:
3046 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4115 1937
Size: 2178 4594
Acc65I G'GTAC_C
Cuts at: 2424 3837 4093 2424
Size: 1413 256 5103
Fragments arranged by size:
5103 1413 256
AclI AA'CG_TT
Cuts at: 1563 4899 5272 1563
Size: 3336 373 3063
Fragments arranged by size:
3336 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2708 2972 4835 6219 6487 1632
Size: 1076 264 1863 1384 268 1917
Fragments arranged by size:
1917 1863 1384 1076 268 264
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6575
AflIII A'CryG_T
Cuts at: 1203 2178 2355 3867 5671 6648 1203
Size: 975 177 1512 1804 977 1327
Fragments arranged by size:
1804 1512 1327 977 975 177
AgeI A'CCGG_T
Cuts at: 5603 5603
Size: 6772
AhdI GACnn_n'nnGTC
Cuts at: 5501 5501
Size: 6772
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6772
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2071 2949 3611 4049 1184
Size: 887 878 662 438 3907
Fragments arranged by size:
3907 887 878 662 438
AlwNI CAG_nnn'CTG
Cuts at: 3067 3171 3526 3694 3933 4515 6087 3067
Size: 104 355 168 239 582 1572 3752
Fragments arranged by size:
3752 1572 582 355 239 168 104
ApaI G_GGCC'C
Cuts at: 2797 2797
Size: 6772
ApaLI G'TGCA_C
Cuts at: 4829 5985 4829
Size: 1156 5616
ApoI r'AATT_y
Cuts at: 1493 4081 4307 4459 6568 1493
Size: 2588 226 152 2109 1697
Fragments arranged by size:
2588 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5326 160
Size: 872 4294 1606
Fragments arranged by size:
4294 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6772
AvaI C'yCGr_G
Cuts at: 1870 1943 2411 2603 3214 3600 4055 4097 1870
Size: 73 468 192 611 386 455 42 4545
Fragments arranged by size:
4545 611 468 455 386 192 73 42
BamHI G'GATC_C
Cuts at: 4102 4469 4102
Size: 367 6405
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2159 2424 3161 3511 3837
Size: 325 217 627 372 265 737 350 326
Cuts at: 3837 4093 5548 618
Size: 256 1455 1842
Fragments arranged by size:
1842 1455 737 627 372 350 326 325
265 256 217
BanII G_rGCy'C
Cuts at: 1391 2124 2601 2797 3132 3609 4056 4091 1391
Size: 733 477 196 335 477 447 35 4072
Fragments arranged by size:
4072 733 477 477 447 335 196 35
BbeI G_GCGC'C
Cuts at: 3165 3515 3165
Size: 350 6422
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5801
BbvCI CC'TCA_GC
Cuts at: 3977 3977
Size: 6772
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2551 2585 3713 3747 4963 4997 1903
Size: 34 614 34 1128 34 1216 34 3678
Fragments arranged by size:
3678 1216 1128 614 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4664 5874 4664
Size: 1210 5562
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6772
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3341 3350 5383
Size: 107 122 71 631 605 1669 9 2033
Cuts at: 5383 136
Size: 1525
Fragments arranged by size:
2033 1669 1525 631 605 122 107 71
9
BglII A'GATC_T
Cuts at: 6767 6767
Size: 6772
BlpI GC'TnA_GC
Cuts at: 2516 4189 2516
Size: 1673 5099
Bme1580I G_kGCm'C
Cuts at: 1790 2095 2797 3276 3600 4833 5989 1790
Size: 305 702 479 324 1233 1156 2573
Fragments arranged by size:
2573 1233 1156 702 479 324 305
BmgBI CAC'GTC
Cuts at: 1318 2179 2332 2356 3184 1318
Size: 861 153 24 828 4906
Fragments arranged by size:
4906 861 828 153 24
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3544 3820 4503
Size: 416 53 83 399 24 2127 276 683
Cuts at: 4503 5461 442
Size: 958 1753
Fragments arranged by size:
2127 1753 958 683 416 399 276 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6772
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2363 2450 2567 2645 2864 2966 3548 3632
Size: 1124 87 117 78 219 102 582 84
Cuts at: 3632 5432 1239
Size: 1800 2579
Fragments arranged by size:
2579 1800 1124 582 219 117 102 87
84 78
Bpu10I CC'TnA_GC
Cuts at: 3977 4022 3977
Size: 45 6727
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2183 2645 2756 3056 4897 5762 6060 2183
Size: 462 111 300 1841 865 298 2895
Fragments arranged by size:
2895 1841 865 462 300 298 111
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2702 3404 3937 5435 882
Size: 617 1203 702 533 1498 2219
Fragments arranged by size:
2219 1498 1203 702 617 533
BsaAI yAC'GTr
Cuts at: 493 1204 2701 3436 3490 4563 493
Size: 711 1497 735 54 1073 2702
Fragments arranged by size:
2702 1497 1073 735 711 54
BsaBI GATnn'nnATC
Cuts at: 3335 6766 3335
Size: 3431 3341
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3162 3512 3822 4011
Size: 53 83 186 936 1629 350 310 189
Cuts at: 4011 4579 4961 5596 275
Size: 568 382 635 1451
Fragments arranged by size:
1629 1451 936 635 568 382 350 310
189 186 83 53
BsaWI w'CCGG_w
Cuts at: 2286 5205 5603 5877 6024 6435 2286
Size: 2919 398 274 147 411 2623
Fragments arranged by size:
2919 2623 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2342 2372 3500 3530 1178
Size: 30 165 30 939 30 1128 30 4420
Fragments arranged by size:
4420 1128 939 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2120 2741 2768 3053 3170 3740 1186
Size: 934 621 27 285 117 570 4218
Fragments arranged by size:
4218 934 621 570 285 117 27
BseYI C'CCAG_C
Cuts at: 2094 2259 2446 2812 3112 5975 6471 2094
Size: 165 187 366 300 2863 496 2395
Fragments arranged by size:
2863 2395 496 366 300 187 165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2046 3110 3680 1163
Size: 573 310 1064 570 4255
Fragments arranged by size:
4255 1064 573 570 310
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2995 4222 4983 6011 1540
Size: 275 186 994 1227 761 1028 2301
Fragments arranged by size:
2301 1227 1028 994 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2060 2124 2195 3132 3609 4833 4918 5989 2060
Size: 64 71 937 477 1224 85 1071 2843
Fragments arranged by size:
2843 1224 1071 937 477 85 71 64
BsmI GAATG_Cn'
Cuts at: 4283 4376 4283
Size: 93 6679
BsmBI CGTCTCn'nnnn_
Cuts at: 3035 3035
Size: 6772
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2060 2095 2124 2195 2601 2797 3132
Size: 399 270 35 29 71 406 196 335
Cuts at: 3132 3276 3600 3609 4056 4091 4833 4918 5989
Size: 144 324 9 447 35 742 85 1071
Cuts at: 5989 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 447 406 399 335 324
270 196 144 85 71 35 35 29
9
BspEI T'CCGG_A
Cuts at: 2286 6435 2286
Size: 4149 2623
BspHI T'CATG_A
Cuts at: 4661 6391 4661
Size: 1730 5042
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2082 3075 3082 3666 3800 4027 4054 4127
Size: 1238 993 7 584 134 227 27 73
Cuts at: 4127 844
Size: 3489
Fragments arranged by size:
3489 1238 993 584 227 134 73 27
7
BsrBI CCG'CTC
Cuts at: 1394 1823 2434 3496 4659 1394
Size: 429 611 1062 1163 3507
Fragments arranged by size:
3507 1163 1062 611 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5267 5441 65
Size: 1183 4019 174 1396
Fragments arranged by size:
4019 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2742 5416 5603 1700
Size: 110 932 2674 187 2869
Fragments arranged by size:
2869 2674 932 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6772
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6772
BssSI C'ACGA_G
Cuts at: 3152 4832 5844 3152
Size: 1680 1012 4080
Fragments arranged by size:
4080 1680 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2024 3109 794
Size: 1230 1085 4457
Fragments arranged by size:
4457 1230 1085
BstBI TT'CG_AA
Cuts at: 5576 5576
Size: 6772
BstEII G'GTnAC_C
Cuts at: 1184 2221 2653 1184
Size: 1037 432 5303
Fragments arranged by size:
5303 1037 432
BstXI CCAn_nnnn'nTGG
Cuts at: 3428 3428
Size: 6772
BstYI r'GATC_y
Cuts at: 1972 3750 4102 4469 4854 4871 6323 6409 6421
Size: 1778 352 367 385 17 1452 86 12
Cuts at: 6421 6767 1972
Size: 346 1977
Fragments arranged by size:
1977 1778 1452 385 367 352 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2874 3254 3345 3861 513
Size: 552 1809 380 91 516 3424
Fragments arranged by size:
3424 1809 552 516 380 91
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3322 487
Size: 683 56 2096 3937
Fragments arranged by size:
3937 2096 683 56
BtsI GCAGTG_nn'
Cuts at: 4288 5082 5102 4288
Size: 794 20 5958
Fragments arranged by size:
5958 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6772
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6737
DraI TTT'AAA
Cuts at: 4076 4076
Size: 6772
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5779 809
Size: 507 4463 1802
Fragments arranged by size:
4463 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2208 2856 2877
Size: 54 1425 50 275 18 378 648 21
Cuts at: 2877 2992 3342 3351 3430 3765 3799 4219 5108
Size: 115 350 9 79 335 34 420 889
Cuts at: 5108 6737 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 648 420 378 350 335
275 115 79 54 50 43 34 21
18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2992 4219 1537
Size: 275 1180 1227 4090
Fragments arranged by size:
4090 1227 1180 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3656 4702 1353
Size: 2303 1046 3423
Fragments arranged by size:
3423 2303 1046
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2054 3476 5359 5733 5879 387
Size: 1064 138 465 1422 1883 374 146 1280
Fragments arranged by size:
1883 1422 1280 1064 465 374 146 138
EcoICRI GAG'CTC
Cuts at: 2122 3130 3607 2122
Size: 1008 477 5287
Fragments arranged by size:
5287 1008 477
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2363 2450 2567 2645 2708 2864 2966
Size: 393 731 87 117 78 63 156 102
Cuts at: 2966 2972 3548 3632 4835 5432 6219 6487 1239
Size: 6 576 84 1203 597 787 268 1524
Fragments arranged by size:
1524 1203 787 731 597 576 393 268
156 117 102 87 84 78 63 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2552 3071 3311 3677 4025 2552
Size: 519 240 366 348 5299
Fragments arranged by size:
5299 519 366 348 240
EcoO109I rG'GnC_Cy
Cuts at: 2083 2977 3483 3691 3760 2083
Size: 894 506 208 69 5095
Fragments arranged by size:
5095 894 506 208 69
EcoRI G'AATT_C
Cuts at: 4081 4459 4081
Size: 378 6394
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3097 3097
Size: 6772
FspI TGC'GCA
Cuts at: 5278 5278
Size: 6772
HaeII r_GCGC'y
Cuts at: 1678 2207 2974 3165 3515 3594 3972 3985 5919
Size: 529 767 191 350 79 378 13 1934
Cuts at: 5919 1678
Size: 2531
Fragments arranged by size:
2531 1934 767 529 378 350 191 79
13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2033 2065 2165 2197 3197
Size: 32 135 32 461 32 100 32 1000
Cuts at: 3197 3229 3368 3400 3500 3532 5413 5445 5487
Size: 32 139 32 100 32 1881 32 42
Cuts at: 5487 5519 1373
Size: 32 2626
Fragments arranged by size:
2626 1881 1000 461 139 135 100 100
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4116 4362 669
Size: 988 281 2178 246 3079
Fragments arranged by size:
3079 2178 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6772
HpaI GTT'AAC
Cuts at: 4362 4362
Size: 6772
KasI G'GCGC_C
Cuts at: 3161 3511 3161
Size: 350 6422
KpnI G_GTAC'C
Cuts at: 2428 3841 4097 2428
Size: 1413 256 5103
Fragments arranged by size:
5103 1413 256
MfeI C'AATT_G
Cuts at: 4371 4371
Size: 6772
MluI A'CGCG_T
Cuts at: 6648 6648
Size: 6772
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5886 6070 1305
Size: 4581 184 2007
Fragments arranged by size:
4581 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2858 2879 3353 3432 10
Size: 54 1425 343 1026 21 474 79 3350
Fragments arranged by size:
3350 1425 1026 474 343 79 54 21
MslI CAynn'nnrTG
Cuts at: 518 1166 2053 3866 4730 5089 5248 518
Size: 648 887 1813 864 359 159 2042
Fragments arranged by size:
2042 1813 887 864 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3256 3480 3556 4188 4865 6013 6258 1606
Size: 1650 224 76 632 677 1148 245 2120
Fragments arranged by size:
2120 1650 1148 677 632 245 224 76
NarI GG'CG_CC
Cuts at: 3162 3512 3162
Size: 350 6422
NcoI C'CATG_G
Cuts at: 513 1065 3345 3861 513
Size: 552 2280 516 3424
Fragments arranged by size:
3424 2280 552 516
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6772
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6772
NotI GC'GGCC_GC
Cuts at: 4219 4219
Size: 6772
NspI r_CATG'y
Cuts at: 1132 1229 2267 3016 3787 3871 4130 5675 1132
Size: 97 1038 749 771 84 259 1545 2229
Fragments arranged by size:
2229 1545 1038 771 749 259 97 84
PasI CC'CwG_GG
Cuts at: 1348 2917 1348
Size: 1569 5203
PciI A'CATG_T
Cuts at: 3867 5671 3867
Size: 1804 4968
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2875 3171 1108
Size: 1767 296 4709
Fragments arranged by size:
4709 1767 296
PfoI T'CCnGG_A
Cuts at: 2379 2583 2841 3609 2379
Size: 204 258 768 5542
Fragments arranged by size:
5542 768 258 204
PmeI GTTT'AAAC
Cuts at: 4076 4076
Size: 6772
PmlI CAC'GTG
Cuts at: 2701 3436 3490 2701
Size: 735 54 5983
Fragments arranged by size:
5983 735 54
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4841 4873 6354 6386 4841
Size: 32 1481 32 5227
Fragments arranged by size:
5227 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2083 3483 3760 2083
Size: 1400 277 5095
Fragments arranged by size:
5095 1400 277
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6772
PsiI TTA'TAA
Cuts at: 4342 4342
Size: 6772
PspOMI G'GGCC_C
Cuts at: 2793 2793
Size: 6772
PstI C_TGCA'G
Cuts at: 830 3297 3396 4038 4124 830
Size: 2467 99 642 86 3478
Fragments arranged by size:
3478 2467 642 99 86
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6772
SacI G_AGCT'C
Cuts at: 2124 3132 3609 2124
Size: 1008 477 5287
Fragments arranged by size:
5287 1008 477
SacII CC_GC'GG
Cuts at: 3257 3257
Size: 6772
SalI G'TCGA_C
Cuts at: 1936 4114 1936
Size: 2178 4594
SanDI GG'GwC_CC
Cuts at: 3483 3760 3483
Size: 277 6495
SbfI CC_TGCA'GG
Cuts at: 4124 4124
Size: 6772
ScaI AGT'ACT
Cuts at: 3080 3830 5020 6732 3080
Size: 750 1190 1712 3120
Fragments arranged by size:
3120 1712 1190 750
SexAI A'CCwGG_T
Cuts at: 2151 3030 2151
Size: 879 5893
SfcI C'TryA_G
Cuts at: 826 1045 3293 3392 4034 4120 5255 5936 6127
Size: 219 2248 99 642 86 1135 681 191
Cuts at: 6127 826
Size: 1471
Fragments arranged by size:
2248 1471 1135 681 642 219 191 99
86
SfiI GGCCn_nnn'nGGCC
Cuts at: 3350 3350
Size: 6772
SfoI GGC'GCC
Cuts at: 3163 3513 3163
Size: 350 6422
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6772
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6772
SmaI CCC'GGG
Cuts at: 1872 3602 4099 1872
Size: 1730 497 4545
Fragments arranged by size:
4545 1730 497
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2198 2660 2771 3071 4876 5777
Size: 197 926 255 462 111 300 1805 901
Cuts at: 5777 6039 820
Size: 262 1553
Fragments arranged by size:
1805 1553 926 901 462 300 262 255
197 111
SnaBI TAC'GTA
Cuts at: 493 4563 493
Size: 4070 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6772
SphI G_CATG'C
Cuts at: 1132 4130 1132
Size: 2998 3774
SspI AAT'ATT
Cuts at: 5 52 4696 5
Size: 47 4644 2081
Fragments arranged by size:
4644 2081 47
StuI AGG'CCT
Cuts at: 1517 2732 3648 1517
Size: 1215 916 4641
Fragments arranged by size:
4641 1215 916
StyI C'CwwG_G
Cuts at: 513 1065 2277 3345 3678 3754 3861 4211 513
Size: 552 1212 1068 333 76 107 350 3074
Fragments arranged by size:
3074 1212 1068 552 350 333 107 76
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2177 3526 4799 4816 4969 5154 5599
Size: 1255 597 1349 1273 17 153 185 445
Cuts at: 5599 325
Size: 1498
Fragments arranged by size:
1498 1349 1273 1255 597 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3078 3828 5018
Size: 274 80 33 51 1443 1101 750 1190
Cuts at: 5018 6730 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1190 1101 750 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6732 6764 6732
Size: 32 6740
Tth111I GACn'n_nGTC
Cuts at: 1316 2837 1316
Size: 1521 5251
XbaI T'CTAG_A
Cuts at: 4108 4108
Size: 6772
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2719 2872 3343 3752 3809 2719
Size: 153 471 409 57 5682
Fragments arranged by size:
5682 471 409 153 57
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6772
XmaI C'CCGG_G
Cuts at: 1870 3600 4097 1870
Size: 1730 497 4545
Fragments arranged by size:
4545 1730 497
XmnI GAAnn'nnTTC
Cuts at: 4901 4901
Size: 6772
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4580 276
Size: 53 83 186 936 3046 2468
Fragments arranged by size:
3046 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SacI SacII SalI SanDI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I Bsu36I DraIII EcoRV FseI FspAI NaeI NgoMIV
NruI NsiI PacI PspXI PsrI PvuII RsrII SapI
SrfI SwaI