Restriction map for
pFN21AE4561
(Circular) (Six-base) MAPSORT of: pFN21AE4561.seq Check: 1836 from: 1 to: 6760
pFN21AE4561 6760 bp
With 182 enzymes: SgfI *
March 28, 2012 14:15 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4570 278
Size: 53 83 186 936 3034 2468
Fragments arranged by size:
3034 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2391 4103 1937
Size: 454 1712 4594
Fragments arranged by size:
4594 1712 454
Acc65I G'GTAC_C
Cuts at: 4081 4081
Size: 6760
AclI AA'CG_TT
Cuts at: 1563 4887 5260 1563
Size: 3324 373 3063
Fragments arranged by size:
3324 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2561 2600 2771 2924 3029 4823 6207 6475
Size: 929 39 171 153 105 1794 1384 268
Cuts at: 6475 1632
Size: 1917
Fragments arranged by size:
1917 1794 1384 929 268 171 153 105
39
AflII C'TTAA_G
Cuts at: 820 1017 4052 820
Size: 197 3035 3528
Fragments arranged by size:
3528 3035 197
AflIII A'CryG_T
Cuts at: 1203 5659 6636 1203
Size: 4456 977 1327
Fragments arranged by size:
4456 1327 977
AgeI A'CCGG_T
Cuts at: 5591 5591
Size: 6760
AhdI GACnn_n'nnGTC
Cuts at: 5489 5489
Size: 6760
AleI CACnn'nnGTG
Cuts at: 1166 2435 3585 1166
Size: 1269 1150 4341
Fragments arranged by size:
4341 1269 1150
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2164 1184
Size: 980 5780
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2696 2728 2696
Size: 32 6728
AlwNI CAG_nnn'CTG
Cuts at: 2425 2534 2572 2776 2902 3124 3511 4503 6075
Size: 109 38 204 126 222 387 992 1572
Cuts at: 6075 2425
Size: 3110
Fragments arranged by size:
3110 1572 992 387 222 204 126 109
38
ApaLI G'TGCA_C
Cuts at: 4817 5973 4817
Size: 1156 5604
ApoI r'AATT_y
Cuts at: 1493 3228 4069 4295 4447 6556 1493
Size: 1735 841 226 152 2109 1697
Fragments arranged by size:
2109 1735 1697 841 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5314 160
Size: 872 4282 1606
Fragments arranged by size:
4282 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6760
AvaI C'yCGr_G
Cuts at: 1870 1943 2110 4085 1870
Size: 73 167 1975 4545
Fragments arranged by size:
4545 1975 167 73
BamHI G'GATC_C
Cuts at: 4090 4457 4090
Size: 367 6393
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3179 4081 5536 618
Size: 325 217 627 1392 902 1455 1842
Fragments arranged by size:
1842 1455 1392 902 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3806 3910 4079 1391
Size: 2415 104 169 4072
Fragments arranged by size:
4072 2415 169 104
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2292 928
Size: 971 393 5396
Fragments arranged by size:
5396 971 393
BbvCI CC'TCA_GC
Cuts at: 2072 2955 3808 3966 2072
Size: 883 853 158 4866
Fragments arranged by size:
4866 883 853 158
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4951 4985 1903
Size: 34 3014 34 3678
Fragments arranged by size:
3678 3014 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2584 4652 5862 2584
Size: 2068 1210 3482
Fragments arranged by size:
3482 2068 1210
BclI T'GATC_A
Cuts at: 1548 2427 1548
Size: 879 5881
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3746 5371 136
Size: 107 122 71 631 605 2074 1625 1525
Fragments arranged by size:
2074 1625 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6755 6755
Size: 6760
BlpI GC'TnA_GC
Cuts at: 2766 2883 3240 3800 4177 2766
Size: 117 357 560 377 5349
Fragments arranged by size:
5349 560 377 357 117
Bme1580I G_kGCm'C
Cuts at: 1790 4821 5977 1790
Size: 3031 1156 2573
Fragments arranged by size:
3031 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3433 1318
Size: 2115 4645
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4491 5449 442
Size: 416 53 83 399 24 3074 958 1753
Fragments arranged by size:
3074 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6760
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3018 3873 3988 5420 1239
Size: 1779 855 115 1432 2579
Fragments arranged by size:
2579 1779 1432 855 115
Bpu10I CC'TnA_GC
Cuts at: 2072 2955 3808 3966 2072
Size: 883 853 158 4866
Fragments arranged by size:
4866 883 853 158
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2621 3007 4885 5750 6048 2621
Size: 386 1878 865 298 3333
Fragments arranged by size:
3333 1878 865 386 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2453 3708 5423 882
Size: 617 954 1255 1715 2219
Fragments arranged by size:
2219 1715 1255 954 617
BsaAI yAC'GTr
Cuts at: 493 1204 3190 4551 493
Size: 711 1986 1361 2702
Fragments arranged by size:
2702 1986 1361 711
BsaBI GATnn'nnATC
Cuts at: 3219 3305 6754 3219
Size: 86 3449 3225
Fragments arranged by size:
3449 3225 86
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4567 4949 5584 275
Size: 53 83 186 936 3034 382 635 1451
Fragments arranged by size:
3034 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2466 2553 5193 5591 5865 6012 6423 2466
Size: 87 2640 398 274 147 411 2803
Fragments arranged by size:
2803 2640 411 398 274 147 87
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2348 2378 2696 2726 3443
Size: 30 165 30 945 30 318 30 717
Cuts at: 3443 3473 3871 3901 1178
Size: 30 398 30 4037
Fragments arranged by size:
4037 945 717 398 318 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 2039 2141 2471 2601 2828 2972 2975
Size: 841 12 102 330 130 227 144 3
Cuts at: 2975 3464 3606 3983 1186
Size: 489 142 377 3963
Fragments arranged by size:
3963 841 489 377 330 227 144 142
130 102 12 3
BseYI C'CCAG_C
Cuts at: 2895 3377 3860 5963 6459 2895
Size: 482 483 2103 496 3196
Fragments arranged by size:
3196 2103 496 483 482
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2039 2783 3269 1163
Size: 573 303 744 486 4654
Fragments arranged by size:
4654 744 573 486 303
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4210 4971 5999 1540
Size: 275 186 2209 761 1028 2301
Fragments arranged by size:
2301 2209 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2601 2724 2820 4821 4906 5977 2601
Size: 123 96 2001 85 1071 3384
Fragments arranged by size:
3384 2001 1071 123 96 85
BsmI GAATG_Cn'
Cuts at: 3933 4271 4364 3933
Size: 338 93 6329
Fragments arranged by size:
6329 338 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2601 2724 2820 3806 3910 4079 4821
Size: 399 811 123 96 986 104 169 742
Cuts at: 4821 4906 5977 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 986 811 742 399 169 123
104 96 85
BspEI T'CCGG_A
Cuts at: 2466 6423 2466
Size: 3957 2803
BspHI T'CATG_A
Cuts at: 3420 4649 6379 3420
Size: 1229 1730 3801
Fragments arranged by size:
3801 1730 1229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2962 4115 844
Size: 2118 1153 3489
Fragments arranged by size:
3489 2118 1153
BsrBI CCG'CTC
Cuts at: 1394 1823 2935 4647 1394
Size: 429 1112 1712 3507
Fragments arranged by size:
3507 1712 1112 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3269 5255 5429 65
Size: 1183 2021 1986 174 1396
Fragments arranged by size:
2021 1986 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3675 5404 5591 1700
Size: 110 1865 1729 187 2869
Fragments arranged by size:
2869 1865 1729 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6760
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6760
BssSI C'ACGA_G
Cuts at: 4820 5832 4820
Size: 1012 5748
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2572 2776 794
Size: 1778 204 4778
Fragments arranged by size:
4778 1778 204
BstBI TT'CG_AA
Cuts at: 5564 5564
Size: 6760
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6760
BstXI CCAn_nnnn'nTGG
Cuts at: 3385 3947 3385
Size: 562 6198
BstYI r'GATC_y
Cuts at: 1972 3306 3536 4090 4457 4842 4859 6311 6397
Size: 1334 230 554 367 385 17 1452 86
Cuts at: 6397 6409 6755 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1334 554 385 367 346 230
86 17 12
BstZ17I GTA'TAC
Cuts at: 2392 2392
Size: 6760
Bsu36I CC'TnA_GG
Cuts at: 2279 2279
Size: 6760
BtgI C'CryG_G
Cuts at: 513 1065 2004 2329 2403 3766 513
Size: 552 939 325 74 1363 3507
Fragments arranged by size:
3507 1363 939 552 325 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2880 487
Size: 683 56 1654 4367
Fragments arranged by size:
4367 1654 683 56
BtsI GCAGTG_nn'
Cuts at: 2133 3502 4276 5070 5090 2133
Size: 1369 774 794 20 3803
Fragments arranged by size:
3803 1369 794 774 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6760
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6725
DraI TTT'AAA
Cuts at: 4064 4064
Size: 6760
DraIII CAC_nnn'GTG
Cuts at: 3821 3821
Size: 6760
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5767 809
Size: 507 4451 1802
Fragments arranged by size:
4451 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2485 3129 3738
Size: 54 1425 50 275 18 655 644 609
Cuts at: 3738 4207 5096 6725 8
Size: 469 889 1629 43
Fragments arranged by size:
1629 1425 889 655 644 609 469 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4207 1537
Size: 275 2395 4090
Fragments arranged by size:
4090 2395 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3155 3208 4690 1353
Size: 1802 53 1482 3423
Fragments arranged by size:
3423 1802 1482 53
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5347 5721 5867 387
Size: 1064 138 3758 374 146 1280
Fragments arranged by size:
3758 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2561 2600 2771 2924 3018 3029 3873
Size: 393 929 39 171 153 94 11 844
Cuts at: 3873 3988 4823 5420 6207 6475 1239
Size: 115 835 597 787 268 1524
Fragments arranged by size:
1524 929 844 835 787 597 393 268
171 153 115 94 39 11
EcoNI CCTnn'n_nnAGG
Cuts at: 2057 2366 2057
Size: 309 6451
EcoO109I rG'GnC_Cy
Cuts at: 2359 3040 2359
Size: 681 6079
EcoRI G'AATT_C
Cuts at: 4069 4447 4069
Size: 378 6382
FspI TGC'GCA
Cuts at: 5266 5266
Size: 6760
HaeII r_GCGC'y
Cuts at: 1678 3283 3511 3761 5907 1678
Size: 1605 228 250 2146 2531
Fragments arranged by size:
2531 2146 1605 250 228
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2996 3028 3443 3445 3475
Size: 32 135 32 1424 32 415 2 30
Cuts at: 3475 3477 3554 3586 3869 3901 3965 3997 5401
Size: 2 77 32 283 32 64 32 1404
Cuts at: 5401 5433 5475 5507 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1424 1404 415 283 135 77 64
42 32 32 32 32 32 32 32
32 30 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3331 4104 4350 669
Size: 988 281 1393 773 246 3079
Fragments arranged by size:
3079 1393 988 773 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6760
HpaI GTT'AAC
Cuts at: 4350 4350
Size: 6760
KpnI G_GTAC'C
Cuts at: 4085 4085
Size: 6760
MfeI C'AATT_G
Cuts at: 2395 4359 2395
Size: 1964 4796
MluI A'CGCG_T
Cuts at: 6636 6636
Size: 6760
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2946 5874 6058 1305
Size: 1641 2928 184 2007
Fragments arranged by size:
2928 2007 1641 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2487 3131 3740 10
Size: 54 1425 343 655 644 609 3030
Fragments arranged by size:
3030 1425 655 644 609 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2351 2435 2522 2651 3585 4718 5077
Size: 648 1185 84 87 129 934 1133 359
Cuts at: 5077 5236 518
Size: 159 2042
Fragments arranged by size:
2042 1185 1133 934 648 359 159 129
87 84
MspA1I CmG'CkG
Cuts at: 1606 2578 2899 3061 3121 3291 3381 4176 4853
Size: 972 321 162 60 170 90 795 677
Cuts at: 4853 6001 6246 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 972 795 677 321 245 170
162 90 60
NcoI C'CATG_G
Cuts at: 513 1065 2004 2329 3766 513
Size: 552 939 325 1437 3507
Fragments arranged by size:
3507 1437 939 552 325
NdeI CA'TA_TG
Cuts at: 387 2434 387
Size: 2047 4713
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6760
NotI GC'GGCC_GC
Cuts at: 4207 4207
Size: 6760
NspI r_CATG'y
Cuts at: 1132 1229 4118 5663 1132
Size: 97 2889 1545 2229
Fragments arranged by size:
2889 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3698 1348
Size: 2350 4410
PciI A'CATG_T
Cuts at: 5659 5659
Size: 6760
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3352 3527 3601 3772 3821 1108
Size: 2244 175 74 171 49 4047
Fragments arranged by size:
4047 2244 175 171 74 49
PmeI GTTT'AAAC
Cuts at: 4064 4064
Size: 6760
PmlI CAC'GTG
Cuts at: 3190 3190
Size: 6760
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2696 2728 4829 4861 6342 6374 2696
Size: 32 2101 32 1481 32 3082
Fragments arranged by size:
3082 2101 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2359 2359
Size: 6760
PshAI GACnn'nnGTC
Cuts at: 1613 2556 1613
Size: 943 5817
PsiI TTA'TAA
Cuts at: 4330 4330
Size: 6760
PstI C_TGCA'G
Cuts at: 830 2140 2478 2577 3060 3492 3637 4112 830
Size: 1310 338 99 483 432 145 475 3478
Fragments arranged by size:
3478 1310 483 475 432 338 145 99
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6760
PvuII CAG'CTG
Cuts at: 2578 3061 3121 3381 2578
Size: 483 60 260 5957
Fragments arranged by size:
5957 483 260 60
SalI G'TCGA_C
Cuts at: 1936 4102 1936
Size: 2166 4594
SapI GCTCTTCn'nnn_
Cuts at: 3208 3208
Size: 6760
SbfI CC_TGCA'GG
Cuts at: 4112 4112
Size: 6760
ScaI AGT'ACT
Cuts at: 5008 6720 5008
Size: 1712 5048
SexAI A'CCwGG_T
Cuts at: 3817 3817
Size: 6760
SfcI C'TryA_G
Cuts at: 826 1045 2136 2474 2573 3056 3488 3633 4108
Size: 219 1091 338 99 483 432 145 475
Cuts at: 4108 5243 5924 6115 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1091 681 483 475 432 338
219 191 145 99
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6760
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6760
SmaI CCC'GGG
Cuts at: 1872 4087 1872
Size: 2215 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2636 2986 4052 4864 5765 6027
Size: 197 926 693 350 1066 812 901 262
Cuts at: 6027 820
Size: 1553
Fragments arranged by size:
1553 1066 926 901 812 693 350 262
197
SnaBI TAC'GTA
Cuts at: 493 4551 493
Size: 4058 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6760
SphI G_CATG'C
Cuts at: 1132 4118 1132
Size: 2986 3774
SspI AAT'ATT
Cuts at: 5 52 3086 4684 5
Size: 47 3034 1598 2081
Fragments arranged by size:
3034 2081 1598 47
StuI AGG'CCT
Cuts at: 1517 2984 1517
Size: 1467 5293
StyI C'CwwG_G
Cuts at: 513 1065 2004 2329 2498 2715 3132 3766 4199
Size: 552 939 325 169 217 417 634 433
Cuts at: 4199 513
Size: 3074
Fragments arranged by size:
3074 939 634 552 433 417 325 217
169
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3711 4787 4804 4957 5142 5587 325
Size: 1255 2131 1076 17 153 185 445 1498
Fragments arranged by size:
2131 1498 1255 1076 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3392 5006 6718
Size: 274 80 33 51 1443 1415 1614 1712
Cuts at: 6718 96
Size: 138
Fragments arranged by size:
1712 1614 1443 1415 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2348 2380 3188 3220 3871 3903 3919 3951 6720
Size: 32 808 32 651 32 16 32 2769
Cuts at: 6720 6752 2348
Size: 32 2356
Fragments arranged by size:
2769 2356 808 651 32 32 32 32
32 16
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6760
XbaI T'CTAG_A
Cuts at: 4096 4096
Size: 6760
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2248 2500 2248
Size: 252 6508
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6760
XmaI C'CCGG_G
Cuts at: 1870 4085 1870
Size: 2215 4545
XmnI GAAnn'nnTTC
Cuts at: 4889 4889
Size: 6760
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4568 276
Size: 53 83 186 936 3034 2468
Fragments arranged by size:
3034 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI EcoICRI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI
SfoI SrfI SwaI